YGR166W / TRS65

Subunit of TRAPPII, a multimeric guanine nucleotide-exchange factor for Ypt1p; involved in intra-Golgi traffic and the retrograde pathway from the endosome to Golgi; role in cell wall beta-glucan biosynthesis and the stress response

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR166W / TRS65

Click on Significant Values for Screen Details ID:SGTC_15|Compound:0266-0086|FD-Score:6.08|P-value:1.70E-8 ID:SGTC_16|Compound:erodoxin|FD-Score:3.89|P-value:2.22E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.87|P-value:2.45E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.02|P-value:6.53E-14 ID:SGTC_77|Compound:0828-0286|FD-Score:4.91|P-value:4.51E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.80|P-value:3.08E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.04|P-value:1.36E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.90|P-value:2.14E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:15.10|P-value:1.37E-43 ID:SGTC_232|Compound:clozapine|FD-Score:5.06|P-value:2.36E-6 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:-3.97|P-value:1.11E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.92|P-value:2.15E-8 ID:SGTC_352|Compound:k048-0007|FD-Score:3.45|P-value:9.63E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:4.22|P-value:6.85E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.46|P-value:9.34E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:4.71|P-value:1.01E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.45|P-value:9.44E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.98|P-value:1.07E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.44|P-value:9.93E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:6.07|P-value:1.83E-8 ID:SGTC_616|Compound:4092-0450|FD-Score:3.84|P-value:2.70E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.87|P-value:1.56E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.33|P-value:9.30E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:5.34|P-value:6.54E-7 ID:SGTC_663|Compound:1486-0733|FD-Score:4.14|P-value:9.32E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:6.56|P-value:1.31E-9 ID:SGTC_800|Compound:3448-7362|FD-Score:6.73|P-value:4.98E-10 ID:SGTC_830|Compound:4076-0286|FD-Score:5.01|P-value:2.90E-6 ID:SGTC_887|Compound:0885-0007|FD-Score:4.77|P-value:7.92E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:5.06|P-value:2.36E-6 ID:SGTC_948|Compound:3448-0295|FD-Score:4.24|P-value:6.47E-5 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.49|P-value:8.47E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.21|P-value:1.18E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:6.59|P-value:1.11E-9 ID:SGTC_1230|Compound:0170-0365|FD-Score:5.74|P-value:9.70E-8 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.55|P-value:4.60E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:5.47|P-value:3.61E-7 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:-3.35|P-value:8.90E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.54|P-value:7.26E-4 ID:SGTC_1672|Compound:st016598|FD-Score:10.70|P-value:7.11E-23 ID:SGTC_1677|Compound:st019204|FD-Score:4.59|P-value:1.65E-5 ID:SGTC_1703|Compound:st026983|FD-Score:-3.38|P-value:8.15E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.02|P-value:1.41E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.83|P-value:2.74E-4 ID:SGTC_1754|Compound:st042065|FD-Score:16.00|P-value:8.61E-49 ID:SGTC_1760|Compound:st045453|FD-Score:6.39|P-value:3.29E-9 ID:SGTC_1766|Compound:st045414|FD-Score:3.56|P-value:6.80E-4 ID:SGTC_1775|Compound:st081588|FD-Score:-3.70|P-value:2.84E-4 ID:SGTC_1797|Compound:st048695|FD-Score:4.81|P-value:6.89E-6 ID:SGTC_1800|Compound:st050079|FD-Score:8.22|P-value:3.74E-14 ID:SGTC_1801|Compound:st050089|FD-Score:11.50|P-value:7.97E-26 ID:SGTC_1803|Compound:st050068|FD-Score:5.60|P-value:1.97E-7 ID:SGTC_1812|Compound:st052057|FD-Score:3.51|P-value:7.83E-4 ID:SGTC_1818|Compound:st052136|FD-Score:14.50|P-value:4.01E-40 ID:SGTC_1989|Compound:st071868|FD-Score:3.68|P-value:4.49E-4 ID:SGTC_1997|Compound:st073024|FD-Score:-3.44|P-value:6.72E-4 ID:SGTC_1999|Compound:st074831|FD-Score:4.29|P-value:5.28E-5 ID:SGTC_2006|Compound:4011487|FD-Score:10.10|P-value:2.57E-20 ID:SGTC_2007|Compound:4011539|FD-Score:6.58|P-value:1.18E-9 ID:SGTC_2009|Compound:4012297|FD-Score:5.31|P-value:7.67E-7 ID:SGTC_2289|Compound:7946404|FD-Score:14.50|P-value:2.68E-40 ID:SGTC_2399|Compound:5884133|FD-Score:3.88|P-value:2.33E-4 ID:SGTC_2429|Compound:5902844|FD-Score:-3.74|P-value:2.48E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-4.19|P-value:4.87E-5 ID:SGTC_2442|Compound:5158906|FD-Score:-4.29|P-value:3.33E-5 ID:SGTC_2464|Compound:5717415|FD-Score:-4.53|P-value:1.26E-5 ID:SGTC_2523|Compound:spermine|FD-Score:4.88|P-value:5.00E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.53|P-value:4.92E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.17|P-value:8.31E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-6.81|P-value:1.54E-10 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-4.94|P-value:2.23E-6 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-4.49|P-value:1.52E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-4.12|P-value:6.45E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:3.58|P-value:6.34E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.49|P-value:5.67E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.64|P-value:5.20E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.75|P-value:3.51E-19 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.65|P-value:5.10E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-4.65|P-value:7.80E-6 ID:SGTC_2696|Compound:st077691|FD-Score:-5.85|P-value:3.04E-8 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.48|P-value:8.64E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.42|P-value:3.26E-5 ID:SGTC_2896|Compound:9052656|FD-Score:12.00|P-value:5.27E-28 ID:SGTC_3054|Compound:9094397|FD-Score:4.14|P-value:9.37E-5 ID:SGTC_3152|Compound:9099253|FD-Score:7.07|P-value:6.62E-11 ID:SGTC_3266|Compound:9136860|FD-Score:-4.53|P-value:1.28E-5 ID:SGTC_15|Compound:0266-0086|FD-Score:6.08|P-value:1.70E-8 ID:SGTC_16|Compound:erodoxin|FD-Score:3.89|P-value:2.22E-4 ID:SGTC_43|Compound:0570-0105|FD-Score:3.87|P-value:2.45E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-8.02|P-value:6.53E-14 ID:SGTC_77|Compound:0828-0286|FD-Score:4.91|P-value:4.51E-6 ID:SGTC_117|Compound:lucigenin|FD-Score:3.80|P-value:3.08E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.04|P-value:1.36E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.90|P-value:2.14E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:15.10|P-value:1.37E-43 ID:SGTC_232|Compound:clozapine|FD-Score:5.06|P-value:2.36E-6 ID:SGTC_241|Compound:5hr heat shock (37°C) + cantharidin|FD-Score:-3.97|P-value:1.11E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-5.92|P-value:2.15E-8 ID:SGTC_352|Compound:k048-0007|FD-Score:3.45|P-value:9.63E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:4.22|P-value:6.85E-5 ID:SGTC_390|Compound:dec-rvkr-cmk|FD-Score:3.46|P-value:9.34E-4 ID:SGTC_439|Compound:amiodarone|FD-Score:4.71|P-value:1.01E-5 ID:SGTC_449|Compound:xestospongin c|FD-Score:3.45|P-value:9.44E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-3.98|P-value:1.07E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.44|P-value:9.93E-4 ID:SGTC_568|Compound:1193-0097|FD-Score:6.07|P-value:1.83E-8 ID:SGTC_616|Compound:4092-0450|FD-Score:3.84|P-value:2.70E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.87|P-value:1.56E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.33|P-value:9.30E-4 ID:SGTC_649|Compound:1123-0311|FD-Score:5.34|P-value:6.54E-7 ID:SGTC_663|Compound:1486-0733|FD-Score:4.14|P-value:9.32E-5 ID:SGTC_708|Compound:3448-5400|FD-Score:6.56|P-value:1.31E-9 ID:SGTC_800|Compound:3448-7362|FD-Score:6.73|P-value:4.98E-10 ID:SGTC_830|Compound:4076-0286|FD-Score:5.01|P-value:2.90E-6 ID:SGTC_887|Compound:0885-0007|FD-Score:4.77|P-value:7.92E-6 ID:SGTC_913|Compound:3487-1245|FD-Score:5.06|P-value:2.36E-6 ID:SGTC_948|Compound:3448-0295|FD-Score:4.24|P-value:6.47E-5 ID:SGTC_1020|Compound:4335-0961|FD-Score:3.49|P-value:8.47E-4 ID:SGTC_1026|Compound:k007-1105|FD-Score:5.21|P-value:1.18E-6 ID:SGTC_1161|Compound:k007-0610|FD-Score:6.59|P-value:1.11E-9 ID:SGTC_1230|Compound:0170-0365|FD-Score:5.74|P-value:9.70E-8 ID:SGTC_1247|Compound:0448-0043|FD-Score:-3.55|P-value:4.60E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:5.47|P-value:3.61E-7 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:-3.35|P-value:8.90E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.54|P-value:7.26E-4 ID:SGTC_1672|Compound:st016598|FD-Score:10.70|P-value:7.11E-23 ID:SGTC_1677|Compound:st019204|FD-Score:4.59|P-value:1.65E-5 ID:SGTC_1703|Compound:st026983|FD-Score:-3.38|P-value:8.15E-4 ID:SGTC_1712|Compound:st032288|FD-Score:4.02|P-value:1.41E-4 ID:SGTC_1750|Compound:st081859|FD-Score:3.83|P-value:2.74E-4 ID:SGTC_1754|Compound:st042065|FD-Score:16.00|P-value:8.61E-49 ID:SGTC_1760|Compound:st045453|FD-Score:6.39|P-value:3.29E-9 ID:SGTC_1766|Compound:st045414|FD-Score:3.56|P-value:6.80E-4 ID:SGTC_1775|Compound:st081588|FD-Score:-3.70|P-value:2.84E-4 ID:SGTC_1797|Compound:st048695|FD-Score:4.81|P-value:6.89E-6 ID:SGTC_1800|Compound:st050079|FD-Score:8.22|P-value:3.74E-14 ID:SGTC_1801|Compound:st050089|FD-Score:11.50|P-value:7.97E-26 ID:SGTC_1803|Compound:st050068|FD-Score:5.60|P-value:1.97E-7 ID:SGTC_1812|Compound:st052057|FD-Score:3.51|P-value:7.83E-4 ID:SGTC_1818|Compound:st052136|FD-Score:14.50|P-value:4.01E-40 ID:SGTC_1989|Compound:st071868|FD-Score:3.68|P-value:4.49E-4 ID:SGTC_1997|Compound:st073024|FD-Score:-3.44|P-value:6.72E-4 ID:SGTC_1999|Compound:st074831|FD-Score:4.29|P-value:5.28E-5 ID:SGTC_2006|Compound:4011487|FD-Score:10.10|P-value:2.57E-20 ID:SGTC_2007|Compound:4011539|FD-Score:6.58|P-value:1.18E-9 ID:SGTC_2009|Compound:4012297|FD-Score:5.31|P-value:7.67E-7 ID:SGTC_2289|Compound:7946404|FD-Score:14.50|P-value:2.68E-40 ID:SGTC_2399|Compound:5884133|FD-Score:3.88|P-value:2.33E-4 ID:SGTC_2429|Compound:5902844|FD-Score:-3.74|P-value:2.48E-4 ID:SGTC_2438|Compound:5259590|FD-Score:-4.19|P-value:4.87E-5 ID:SGTC_2442|Compound:5158906|FD-Score:-4.29|P-value:3.33E-5 ID:SGTC_2464|Compound:5717415|FD-Score:-4.53|P-value:1.26E-5 ID:SGTC_2523|Compound:spermine|FD-Score:4.88|P-value:5.00E-6 ID:SGTC_2569|Compound:tetrandrine|FD-Score:-3.53|P-value:4.92E-4 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.17|P-value:8.31E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:-6.81|P-value:1.54E-10 ID:SGTC_2594|Compound:a-mangostin|FD-Score:-4.94|P-value:2.23E-6 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-4.49|P-value:1.52E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:-4.12|P-value:6.45E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:3.58|P-value:6.34E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:-3.49|P-value:5.67E-4 ID:SGTC_2687|Compound:chenodiol|FD-Score:3.64|P-value:5.20E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:9.75|P-value:3.51E-19 ID:SGTC_2689|Compound:tacrolimus|FD-Score:3.65|P-value:5.10E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-4.65|P-value:7.80E-6 ID:SGTC_2696|Compound:st077691|FD-Score:-5.85|P-value:3.04E-8 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.48|P-value:8.64E-4 ID:SGTC_2890|Compound:9046729|FD-Score:4.42|P-value:3.26E-5 ID:SGTC_2896|Compound:9052656|FD-Score:12.00|P-value:5.27E-28 ID:SGTC_3054|Compound:9094397|FD-Score:4.14|P-value:9.37E-5 ID:SGTC_3152|Compound:9099253|FD-Score:7.07|P-value:6.62E-11 ID:SGTC_3266|Compound:9136860|FD-Score:-4.53|P-value:1.28E-5

Top fitness defect scores for YGR166W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1754 st042065 16.00 16.1 µM 8.61E-49 NEO1-PIK1 8 4.02E-58
2 SGTC_221 trifluoperazine 15.10 10.3 µM 1.37E-43 NEO1-PIK1 23 6.53E-52
3 SGTC_2289 7946404 14.50 165.1 µM 2.68E-40 2 5.61E-48
4 SGTC_1818 st052136 14.50 56.3 µM 4.01E-40 NEO1-PIK1 1 9.07E-48
5 SGTC_2896 9052656 12.00 71.4 µM 5.27E-28 endomembrane recycling 6 2.74E-33
6 SGTC_1801 st050089 11.50 28.4 µM 7.97E-26 NEO1-PIK1 10 1.10E-30
7 SGTC_1672 st016598 10.70 6.5 µM 7.11E-23 endomembrane recycling 7 3.71E-27
8 SGTC_2006 4011487 10.10 200.0 µM 2.57E-20 endomembrane recycling 19 4.24E-24
9 SGTC_2688 kanamycin b 9.75 96.7 µM 3.51E-19 calcium & mitochondrial duress 28 9.66E-23
10 SGTC_1800 st050079 8.22 29.1 µM 3.74E-14 NEO1-PIK1 9 9.94E-17
11 SGTC_3152 9099253 7.07 49.5 µM 6.62E-11 NEO1-PIK1 41 7.65E-13
12 SGTC_800 3448-7362 6.73 155.0 µM 4.98E-10 translation 31 8.56E-12
13 SGTC_1161 k007-0610 6.59 110.0 µM 1.11E-9 ERAD & cell cycle 45 2.24E-11
14 SGTC_2007 4011539 6.58 71.8 µM 1.18E-9 cell wall signaling 21 2.41E-11
15 SGTC_708 3448-5400 6.56 146.0 µM 1.31E-9 7 2.72E-11
16 SGTC_1760 st045453 6.39 10.4 µM 3.29E-9 NEO1-PIK1 77 8.19E-11
17 SGTC_15 0266-0086 6.08 27.1 µM 1.70E-8 endomembrane recycling 19 5.83E-10
18 SGTC_568 1193-0097 6.07 7.5 µM 1.83E-8 ERAD & cell cycle 59 6.37E-10
19 SGTC_1230 0170-0365 5.74 3.8 µM 9.70E-8 endomembrane recycling 24 4.70E-9
20 SGTC_1803 st050068 5.60 52.4 µM 1.97E-7 NEO1-PIK1 94 1.09E-8

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.457 2.08E-172 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.431 3.72E-152 YOR115C TRS33 hom One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.428 1.40E-149 YML037C_p YML037C_p hom Putative protein of unknown function with some characteristics of a transcriptional activator; may be a target of Dbf2p-Mob1p kinase; GFP-fusion protein co-localizes with clathrin-coated vesicles; YML037C is not an essential gene
0.406 2.29E-133 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p
0.403 3.66E-131 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.388 9.54E-121 YEL048C TCA17 hom Subunit of TRAPPII, a multimeric GEF involved in intra-Golgi and endosome-to-Golgi transport; promotes association of TRAPPII-specific subunits with the core complex; sedlin related; human Sedlin mutations cause SEDT, a skeletal disorder
0.374 3.69E-112 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.369 8.98E-109 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.364 2.00E-105 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.359 1.05E-102 YGL199C_d YGL199C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W
0.351 1.14E-97 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.349 9.43E-97 YHR108W GGA2 hom Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin
0.338 1.34E-90 YLR057W MNL2 hom Putative mannosidase involved in ER-associated protein degradation; localizes to the endoplasmic reticulum; sequence similarity with seven-hairpin glycosidase (GH47) family members, such as Mns1p and Mnl1p, that hydrolyze 1,2-linked alpha-D-mannose residues; non-essential gene
0.328 1.01E-84 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.327 2.07E-84 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases