YGR175C / ERG1

Squalene epoxidase, catalyzes the epoxidation of squalene to 2,3-oxidosqualene; plays an essential role in the ergosterol-biosynthesis pathway and is the specific target of the antifungal drug terbinafine

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR175C / ERG1

Click on Significant Values for Screen Details ID:SGTC_57|Compound:miconazole|FD-Score:4.53|P-value:1.63E-6 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.88|P-value:4.68E-5 ID:SGTC_106|Compound:0092-0003|FD-Score:6.66|P-value:4.57E-12 ID:SGTC_109|Compound:0132-0036|FD-Score:3.89|P-value:3.08E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.01|P-value:2.19E-7 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.27|P-value:5.06E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.72|P-value:6.40E-5 ID:SGTC_276|Compound:colchicine|FD-Score:4.11|P-value:1.20E-5 ID:SGTC_378|Compound:3970-0959|FD-Score:3.01|P-value:9.48E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.88|P-value:4.62E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.48|P-value:2.37E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:4.71|P-value:6.58E-7 ID:SGTC_728|Compound:3030-5151|FD-Score:3.80|P-value:4.63E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:-3.50|P-value:2.16E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:3.06|P-value:8.02E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:-5.07|P-value:1.57E-7 ID:SGTC_803|Compound:4488-1585|FD-Score:4.20|P-value:7.97E-6 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.03|P-value:8.89E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:6.85|P-value:1.11E-12 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.05|P-value:1.56E-5 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.10|P-value:6.87E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.24|P-value:4.09E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:-4.43|P-value:3.96E-6 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.70|P-value:6.86E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.52|P-value:1.44E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.35|P-value:2.71E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.60|P-value:1.05E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.08|P-value:7.45E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-3.24|P-value:5.76E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:-3.10|P-value:9.35E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.35|P-value:2.74E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.34|P-value:2.90E-4 ID:SGTC_1767|Compound:st044992|FD-Score:3.32|P-value:3.06E-4 ID:SGTC_1802|Compound:st050112|FD-Score:3.63|P-value:9.22E-5 ID:SGTC_1805|Compound:st050013|FD-Score:3.24|P-value:4.12E-4 ID:SGTC_1808|Compound:st050063|FD-Score:-3.54|P-value:1.87E-4 ID:SGTC_1815|Compound:st051655|FD-Score:4.38|P-value:3.34E-6 ID:SGTC_2052|Compound:5265182|FD-Score:4.03|P-value:1.71E-5 ID:SGTC_2063|Compound:5246145|FD-Score:3.16|P-value:5.63E-4 ID:SGTC_2151|Compound:5563267|FD-Score:4.32|P-value:4.50E-6 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.27|P-value:3.73E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.80|P-value:6.53E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.28|P-value:3.63E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.56|P-value:1.20E-4 ID:SGTC_2327|Compound:6189656|FD-Score:6.97|P-value:4.83E-13 ID:SGTC_2402|Compound:5553605|FD-Score:3.75|P-value:5.51E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.17|P-value:5.30E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.44|P-value:3.82E-6 ID:SGTC_2439|Compound:5492030|FD-Score:4.73|P-value:5.95E-7 ID:SGTC_2441|Compound:5730946|FD-Score:-3.12|P-value:8.78E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.67|P-value:7.75E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.65|P-value:1.22E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.01|P-value:9.41E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.47|P-value:2.42E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:11.20|P-value:1.73E-30 ID:SGTC_2776|Compound:terbinafine|FD-Score:23.70|P-value:1.77E-129 ID:SGTC_2964|Compound:9083345|FD-Score:-3.35|P-value:3.79E-4 ID:SGTC_2967|Compound:9089538|FD-Score:4.82|P-value:3.74E-7 ID:SGTC_3062|Compound:9077494|FD-Score:3.86|P-value:3.59E-5 ID:SGTC_3137|Compound:9097352|FD-Score:4.61|P-value:1.06E-6 ID:SGTC_3299|Compound:9121982|FD-Score:4.46|P-value:2.25E-6 ID:SGTC_3302|Compound:9125395|FD-Score:3.75|P-value:5.53E-5 ID:SGTC_3308|Compound:9128332|FD-Score:4.80|P-value:4.11E-7 ID:SGTC_57|Compound:miconazole|FD-Score:4.53|P-value:1.63E-6 ID:SGTC_71|Compound:0417-1750|FD-Score:-3.88|P-value:4.68E-5 ID:SGTC_106|Compound:0092-0003|FD-Score:6.66|P-value:4.57E-12 ID:SGTC_109|Compound:0132-0036|FD-Score:3.89|P-value:3.08E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-5.01|P-value:2.19E-7 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.27|P-value:5.06E-4 ID:SGTC_154|Compound:3389-1638|FD-Score:3.72|P-value:6.40E-5 ID:SGTC_276|Compound:colchicine|FD-Score:4.11|P-value:1.20E-5 ID:SGTC_378|Compound:3970-0959|FD-Score:3.01|P-value:9.48E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.88|P-value:4.62E-5 ID:SGTC_630|Compound:4356-0227|FD-Score:-3.48|P-value:2.37E-4 ID:SGTC_689|Compound:0833-0039|FD-Score:4.71|P-value:6.58E-7 ID:SGTC_728|Compound:3030-5151|FD-Score:3.80|P-value:4.63E-5 ID:SGTC_737|Compound:1950-0117|FD-Score:-3.50|P-value:2.16E-4 ID:SGTC_751|Compound:0833-0568|FD-Score:3.06|P-value:8.02E-4 ID:SGTC_800|Compound:3448-7362|FD-Score:-5.07|P-value:1.57E-7 ID:SGTC_803|Compound:4488-1585|FD-Score:4.20|P-value:7.97E-6 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.03|P-value:8.89E-4 ID:SGTC_1089|Compound:terbinafine|FD-Score:6.85|P-value:1.11E-12 ID:SGTC_1107|Compound:amlodipine|FD-Score:4.05|P-value:1.56E-5 ID:SGTC_1144|Compound:3486-0183|FD-Score:3.10|P-value:6.87E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:3.24|P-value:4.09E-4 ID:SGTC_1192|Compound:1488-0095|FD-Score:-4.43|P-value:3.96E-6 ID:SGTC_1203|Compound:3992-3522|FD-Score:3.70|P-value:6.86E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.52|P-value:1.44E-4 ID:SGTC_1259|Compound:0671-0132|FD-Score:3.35|P-value:2.71E-4 ID:SGTC_1314|Compound:1222-0010|FD-Score:3.60|P-value:1.05E-4 ID:SGTC_1418|Compound:3966-3099|FD-Score:3.08|P-value:7.45E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:-3.24|P-value:5.76E-4 ID:SGTC_1472|Compound:k841-0093|FD-Score:-3.10|P-value:9.35E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.35|P-value:2.74E-4 ID:SGTC_1758|Compound:st042942|FD-Score:3.34|P-value:2.90E-4 ID:SGTC_1767|Compound:st044992|FD-Score:3.32|P-value:3.06E-4 ID:SGTC_1802|Compound:st050112|FD-Score:3.63|P-value:9.22E-5 ID:SGTC_1805|Compound:st050013|FD-Score:3.24|P-value:4.12E-4 ID:SGTC_1808|Compound:st050063|FD-Score:-3.54|P-value:1.87E-4 ID:SGTC_1815|Compound:st051655|FD-Score:4.38|P-value:3.34E-6 ID:SGTC_2052|Compound:5265182|FD-Score:4.03|P-value:1.71E-5 ID:SGTC_2063|Compound:5246145|FD-Score:3.16|P-value:5.63E-4 ID:SGTC_2151|Compound:5563267|FD-Score:4.32|P-value:4.50E-6 ID:SGTC_2230|Compound:bromochlorosalicylanilide|FD-Score:3.27|P-value:3.73E-4 ID:SGTC_2241|Compound:6772625|FD-Score:-3.80|P-value:6.53E-5 ID:SGTC_2246|Compound:7293507|FD-Score:3.28|P-value:3.63E-4 ID:SGTC_2302|Compound:7771753|FD-Score:3.56|P-value:1.20E-4 ID:SGTC_2327|Compound:6189656|FD-Score:6.97|P-value:4.83E-13 ID:SGTC_2402|Compound:5553605|FD-Score:3.75|P-value:5.51E-5 ID:SGTC_2410|Compound:aloe-emodin-8-o-glycoside|FD-Score:3.17|P-value:5.30E-4 ID:SGTC_2415|Compound:st077333|FD-Score:-4.44|P-value:3.82E-6 ID:SGTC_2439|Compound:5492030|FD-Score:4.73|P-value:5.95E-7 ID:SGTC_2441|Compound:5730946|FD-Score:-3.12|P-value:8.78E-4 ID:SGTC_2595|Compound:hexamethylquercetagetin|FD-Score:3.67|P-value:7.75E-5 ID:SGTC_2601|Compound:securinine|FD-Score:-3.65|P-value:1.22E-4 ID:SGTC_2634|Compound:4'-methoxyflavone|FD-Score:3.01|P-value:9.41E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:-3.47|P-value:2.42E-4 ID:SGTC_2736|Compound:butenafine|FD-Score:11.20|P-value:1.73E-30 ID:SGTC_2776|Compound:terbinafine|FD-Score:23.70|P-value:1.77E-129 ID:SGTC_2964|Compound:9083345|FD-Score:-3.35|P-value:3.79E-4 ID:SGTC_2967|Compound:9089538|FD-Score:4.82|P-value:3.74E-7 ID:SGTC_3062|Compound:9077494|FD-Score:3.86|P-value:3.59E-5 ID:SGTC_3137|Compound:9097352|FD-Score:4.61|P-value:1.06E-6 ID:SGTC_3299|Compound:9121982|FD-Score:4.46|P-value:2.25E-6 ID:SGTC_3302|Compound:9125395|FD-Score:3.75|P-value:5.53E-5 ID:SGTC_3308|Compound:9128332|FD-Score:4.80|P-value:4.11E-7

Top fitness defect scores for YGR175C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2776 terbinafine 23.70 19.9 µM 1.77E-129 cell wall signaling 1 1.41E-124 17.60
2 SGTC_2736 butenafine 11.20 14.7 µM 1.73E-30 1 2.81E-29 4.14
3 SGTC_2327 6189656 6.97 200.0 µM 4.83E-13 1 1.64E-12 1.05
4 SGTC_1089 terbinafine 6.85 2.2 µM 1.11E-12 1 3.63E-12 0.95
5 SGTC_106 0092-0003 6.66 11.6 µM 4.57E-12 copper-dependent oxidative stress 3 1.41E-11 1.55
6 SGTC_2967 9089538 4.82 49.9 µM 3.74E-7 7 7.27E-7 0.12
7 SGTC_3308 9128332 4.80 17.6 µM 4.11E-7 plasma membrane duress 5 7.97E-7 0.29
8 SGTC_2439 5492030 4.73 7.2 µM 5.95E-7 2 1.13E-6 0.28
9 SGTC_689 0833-0039 4.71 98.0 µM 6.58E-7 ergosterol depletion effects on membrane 3 1.25E-6 0.15
10 SGTC_3137 9097352 4.61 49.5 µM 1.06E-6 2 1.97E-6 0.28
11 SGTC_57 miconazole 4.53 162.5 nM 1.63E-6 ergosterol depletion effects on membrane 21 2.98E-6 0.13
12 SGTC_3299 9121982 4.46 43.1 µM 2.25E-6 5 4.06E-6 0.26
13 SGTC_1815 st051655 4.38 58.2 µM 3.34E-6 TSC3-RPN4 11 5.92E-6 0.08
14 SGTC_2151 5563267 4.32 85.4 µM 4.50E-6 14 7.89E-6 0.11
15 SGTC_803 4488-1585 4.20 147.0 µM 7.97E-6 3 1.36E-5 0.37
16 SGTC_276 colchicine 4.11 750.0 µM 1.20E-5 2 2.02E-5 0.41
17 SGTC_1107 amlodipine 4.05 47.0 µM 1.56E-5 5 2.59E-5 0.17
18 SGTC_2052 5265182 4.03 7.1 µM 1.71E-5 6 2.82E-5 0.08
19 SGTC_109 0132-0036 3.89 33.7 µM 3.08E-5 3 4.97E-5 0.39
20 SGTC_3062 9077494 3.86 49.5 µM 3.59E-5 mitochondrial response to ROS 8 5.75E-5 0.14

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.339 8.82E-91 YGR176W_d YGR176W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.182 1.71E-26 YER083C GET2 hom Subunit of the GET complex; involved in insertion of proteins into the ER membrane; required for the retrieval of HDEL proteins from the Golgi to the ER in an ERD2 dependent fashion and for meiotic nuclear division
0.140 4.18E-16 YNL288W CAF40 hom Evolutionarily conserved subunit of the CCR4-NOT complex involved in controlling mRNA initiation, elongation and degradation; binds Cdc39p
0.119 4.34E-12 YKL026C GPX1 hom Phospholipid hydroperoxide glutathione peroxidase induced by glucose starvation that protects cells from phospholipid hydroperoxides and nonphospholipid peroxides during oxidative stress
0.118 6.30E-12 YGR279C SCW4 hom Cell wall protein with similarity to glucanases; scw4 scw10 double mutants exhibit defects in mating
0.117 1.14E-11 YKL217W JEN1 hom Monocarboxylate/proton symporter of the plasma membrane; transport activity is dependent on the pH gradient across the membrane; mediates high-affinity uptake of carbon sources lactate, pyuvate, and acetate, and also of the micronutrient selenite, whose structure mimics that of monocarboxylates; expression and localization are tightly regulated, with transcription repression, mRNA degradation, and protein endocytosis and degradation all occurring in the presence of glucose
0.115 3.21E-11 YHR030C SLT2 hom Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.113 5.45E-11 YBR036C CSG2 hom Endoplasmic reticulum membrane protein, required for mannosylation of inositolphosphorylceramide and for growth at high calcium concentrations; protein abundance increases in response to DNA replication stress
0.108 3.26E-10 YAL009W SPO7 hom Putative regulatory subunit of Nem1p-Spo7p phosphatase holoenzyme, regulates nuclear growth by controlling phospholipid biosynthesis, required for normal nuclear envelope morphology, premeiotic replication, and sporulation
0.108 3.30E-10 YPL057C SUR1 hom Mannosylinositol phosphorylceramide (MIPC) synthase catalytic subunit; forms a complex with regulatory subunit Csg2p; function in sphingolipid biosynthesis is overlapping with that of Csh1p; SUR1 has a paralog, CSH1, that arose from the whole genome duplication
0.104 1.36E-9 YLL058W_p YLL058W_p hom Putative protein of unknown function with similarity to Str2p, which is a cystathionine gamma-synthase important in sulfur metabolism; YLL058W is not an essential gene
0.103 1.90E-9 YHR117W TOM71 hom Mitochondrial outer membrane protein; probable minor component of the TOM (translocase of outer membrane) complex responsible for recognition and import of mitochondrially directed proteins; TOM71 has a paralog, TOM70, that arose from the whole genome duplication
0.100 6.54E-9 YML049C RSE1 het Protein involved in pre-mRNA splicing; component of the pre-spliceosome; associates with U2 snRNA; involved in ER to Golgi transport
0.099 8.41E-9 YCR092C MSH3 hom Mismatch repair protein, forms dimers with Msh2p that mediate repair of insertion or deletion mutations and removal of nonhomologous DNA ends, contains a PCNA (Pol30p) binding motif required for genome stability
0.098 1.13E-8 YGL026C TRP5 hom Tryptophan synthase, catalyzes the last step of tryptophan biosynthesis; regulated by the general control system of amino acid biosynthesis