YGR185C / TYS1

Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR185C / TYS1

Click on Significant Values for Screen Details ID:SGTC_485|Compound:niflumic acid|FD-Score:2.95|P-value:1.33E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:-3.15|P-value:7.98E-5 ID:SGTC_548|Compound:1436-0017|FD-Score:3.99|P-value:4.61E-7 ID:SGTC_646|Compound:4092-0839|FD-Score:4.33|P-value:5.10E-8 ID:SGTC_670|Compound:4204-0025|FD-Score:2.81|P-value:2.56E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.68|P-value:6.93E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:2.52|P-value:8.78E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:2.58|P-value:6.99E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.77|P-value:3.06E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:3.26|P-value:2.80E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:2.77|P-value:3.01E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-5.33|P-value:6.76E-11 ID:SGTC_916|Compound:4466-0038|FD-Score:3.02|P-value:9.51E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-7.67|P-value:9.81E-21 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.00|P-value:1.70E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.53|P-value:1.30E-11 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.06|P-value:1.27E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:4.01|P-value:4.01E-7 ID:SGTC_1212|Compound:0520-0165|FD-Score:2.70|P-value:4.19E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:-2.86|P-value:3.11E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-2.99|P-value:1.77E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.56|P-value:7.49E-4 ID:SGTC_2117|Compound:5521686|FD-Score:-2.60|P-value:9.72E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-2.89|P-value:2.81E-4 ID:SGTC_2245|Compound:6637752|FD-Score:8.48|P-value:1.67E-25 ID:SGTC_2309|Compound:7664088|FD-Score:2.97|P-value:1.18E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.77|P-value:2.23E-9 ID:SGTC_2318|Compound:6029776|FD-Score:6.08|P-value:4.54E-14 ID:SGTC_2321|Compound:7390090|FD-Score:3.62|P-value:4.05E-6 ID:SGTC_2324|Compound:6149169|FD-Score:3.90|P-value:7.93E-7 ID:SGTC_2433|Compound:5607874|FD-Score:-2.83|P-value:3.67E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.00|P-value:1.68E-4 ID:SGTC_2570|Compound:licareol|FD-Score:-2.76|P-value:4.92E-4 ID:SGTC_2918|Compound:7977171|FD-Score:2.72|P-value:3.70E-4 ID:SGTC_3168|Compound:9103419|FD-Score:2.51|P-value:9.27E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.24|P-value:3.16E-5 ID:SGTC_485|Compound:niflumic acid|FD-Score:2.95|P-value:1.33E-4 ID:SGTC_544|Compound:k015-0020|FD-Score:-3.15|P-value:7.98E-5 ID:SGTC_548|Compound:1436-0017|FD-Score:3.99|P-value:4.61E-7 ID:SGTC_646|Compound:4092-0839|FD-Score:4.33|P-value:5.10E-8 ID:SGTC_670|Compound:4204-0025|FD-Score:2.81|P-value:2.56E-4 ID:SGTC_750|Compound:4073-0104|FD-Score:-2.68|P-value:6.93E-4 ID:SGTC_762|Compound:1165-0429|FD-Score:2.52|P-value:8.78E-4 ID:SGTC_766|Compound:1391-0721|FD-Score:2.58|P-value:6.99E-4 ID:SGTC_875|Compound:0709-0210|FD-Score:2.77|P-value:3.06E-4 ID:SGTC_884|Compound:0851-0618|FD-Score:3.26|P-value:2.80E-5 ID:SGTC_889|Compound:0919-1524|FD-Score:2.77|P-value:3.01E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:-5.33|P-value:6.76E-11 ID:SGTC_916|Compound:4466-0038|FD-Score:3.02|P-value:9.51E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-7.67|P-value:9.81E-21 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.00|P-value:1.70E-4 ID:SGTC_1107|Compound:amlodipine|FD-Score:-5.53|P-value:1.30E-11 ID:SGTC_1109|Compound:tnp00067|FD-Score:-3.06|P-value:1.27E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:4.01|P-value:4.01E-7 ID:SGTC_1212|Compound:0520-0165|FD-Score:2.70|P-value:4.19E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:-2.86|P-value:3.11E-4 ID:SGTC_1724|Compound:st037281|FD-Score:-2.99|P-value:1.77E-4 ID:SGTC_2115|Compound:tiliquinol|FD-Score:2.56|P-value:7.49E-4 ID:SGTC_2117|Compound:5521686|FD-Score:-2.60|P-value:9.72E-4 ID:SGTC_2238|Compound:6671620|FD-Score:-2.89|P-value:2.81E-4 ID:SGTC_2245|Compound:6637752|FD-Score:8.48|P-value:1.67E-25 ID:SGTC_2309|Compound:7664088|FD-Score:2.97|P-value:1.18E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.77|P-value:2.23E-9 ID:SGTC_2318|Compound:6029776|FD-Score:6.08|P-value:4.54E-14 ID:SGTC_2321|Compound:7390090|FD-Score:3.62|P-value:4.05E-6 ID:SGTC_2324|Compound:6149169|FD-Score:3.90|P-value:7.93E-7 ID:SGTC_2433|Compound:5607874|FD-Score:-2.83|P-value:3.67E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.00|P-value:1.68E-4 ID:SGTC_2570|Compound:licareol|FD-Score:-2.76|P-value:4.92E-4 ID:SGTC_2918|Compound:7977171|FD-Score:2.72|P-value:3.70E-4 ID:SGTC_3168|Compound:9103419|FD-Score:2.51|P-value:9.27E-4 ID:SGTC_3328|Compound:9140624|FD-Score:3.24|P-value:3.16E-5

Top fitness defect scores for YGR185C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2245 6637752 8.48 200.0 µM 1.67E-25 1 1.15E-17 3.87
2 SGTC_2318 6029776 6.08 164.0 µM 4.54E-14 1 6.07E-10 2.14
3 SGTC_2316 5844804 4.77 197.4 µM 2.23E-9 9 9.05E-7 0.32
4 SGTC_646 4092-0839 4.33 27.3 µM 5.10E-8 plasma membrane duress 6 7.56E-6 0.09
5 SGTC_1136 pergolide 4.01 1.1 µM 4.01E-7 14 3.07E-5 0.01
6 SGTC_548 1436-0017 3.99 110.0 µM 4.61E-7 TSC3-RPN4 7 3.37E-5 0.26
7 SGTC_2324 6149169 3.90 200.0 µM 7.93E-7 5 4.87E-5 0.18
8 SGTC_2321 7390090 3.62 138.8 µM 4.05E-6 8 1.48E-4 0.11
9 SGTC_884 0851-0618 3.26 334.0 µM 2.80E-5 11 5.51E-4 0.01
10 SGTC_3328 9140624 3.24 29.0 µM 3.16E-5 10 5.99E-4 0.14
11 SGTC_916 4466-0038 3.02 42.0 nM 9.51E-5 cell wall 44 0.00127 0.03
12 SGTC_2309 7664088 2.97 163.9 µM 1.18E-4 21 0.00148 9.07E-4
13 SGTC_485 niflumic acid 2.95 177.0 µM 1.33E-4 10 0.00160 0.03
14 SGTC_670 4204-0025 2.81 119.0 µM 2.56E-4 copper-dependent oxidative stress 8 0.00250 0.00
15 SGTC_889 0919-1524 2.77 82.7 µM 3.01E-4 11 0.00280 0.07
16 SGTC_875 0709-0210 2.77 25.7 µM 3.06E-4 fatty acid desaturase (OLE1) 29 0.00282 0.05
17 SGTC_2918 7977171 2.72 22.1 µM 3.70E-4 16 0.00322 0.00
18 SGTC_1212 0520-0165 2.70 585.0 µM 4.19E-4 27 0.00351 0.04
19 SGTC_766 1391-0721 2.58 126.0 µM 6.99E-4 27 0.00498 0.07
20 SGTC_2115 tiliquinol 2.56 12.8 µM 7.49E-4 25 0.00522 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.204 5.50E-33 YPL121C MEI5 hom Meiosis specific protein involved in DMC1-dependent meiotic recombination, forms heterodimer with Sae3p; proposed to be an assembly factor for Dmc1p
0.186 1.43E-27 YER011W TIR1 hom Cell wall mannoprotein of the Srp1p/Tip1p family of serine-alanine-rich proteins; expression is downregulated at acidic pH and induced by cold shock and anaerobiosis; abundance is increased in cells cultured without shaking
0.176 1.04E-24 YER064C VHR2 hom Non-essential nuclear protein; null mutation has global effects on transcription; VHR2 has a paralog, VHR1, that arose from the whole genome duplication; relative distribution to the nucleus increases upon DNA replication stress
0.171 2.76E-23 YGR216C GPI1 het Membrane protein involved in the synthesis of N-acetylglucosaminyl phosphatidylinositol (GlcNAc-PI), the first intermediate in the synthesis of glycosylphosphatidylinositol (GPI) anchors; human and mouse GPI1p are functional homologs
0.168 1.56E-22 YML083C YML083C hom Protein of unknown function; transcriptionally regulated by Upc2p via an upstream sterol response element; strong increase in transcript abundance during anaerobic growth compared to aerobic growth; cells deleted for YML083C do not exhibit growth defects in anerobic or anaerobic conditions
0.160 9.20E-21 YDR122W KIN1 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin2p
0.159 1.82E-20 YDL060W TSR1 het Protein required for processing of 20S pre-rRNA in the cytoplasm; associates with pre-40S ribosomal particles; inhibits the premature association of 60S subunits with assembling 40S subunits in the cytoplasm; similar to Bms1p; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.157 8.37E-20 YDR428C BNA7 hom Formylkynurenine formamidase, involved in the de novo biosynthesis of NAD from tryptophan via kynurenine
0.154 2.65E-19 YGR149W_p YGR149W_p hom Putative protein of unknown function; predicted to be an integal membrane protein
0.154 2.97E-19 YDR499W LCD1 het Essential protein required for the DNA integrity checkpoint pathways; interacts physically with Mec1p; putative homolog of S. pombe Rad26 and human ATRIP; forms nuclear foci upon DNA replication stress
0.150 2.54E-18 YNL042W BOP3 hom Protein of unknown function, potential Cdc28p substrate; overproduction confers resistance to methylmercury
0.144 4.36E-17 YOR298C-A MBF1 hom Transcriptional coactivator; bridges the DNA-binding region of Gcn4p and TATA-binding protein Spt15p; suppressor of frameshift mutations; protein abundance increases in response to DNA replication stress
0.144 4.44E-17 YLL035W GRC3 het Polynucleotide kinase present on rDNA that is required for efficient transcription termination by RNA polymerase I; required for cell growth; mRNA is cell-cycle regulated
0.144 5.29E-17 YLR323C CWC24 het Spliceosome assembly factor, essential for the first step of splicing; component of the pre-catalytic spliceosome complex containing Cef1p; has similarity to S. pombe Cwf24p
0.143 9.15E-17 YDL086W_p YDL086W_p hom Putative carboxymethylenebutenolidase; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; YDL086W is not an essential gene