YGR186W / TFG1

TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR186W / TFG1

Click on Significant Values for Screen Details ID:SGTC_173|Compound:1273-0061|FD-Score:3.37|P-value:5.59E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.70|P-value:1.68E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.35|P-value:4.72E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.25|P-value:8.20E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.99|P-value:6.39E-7 ID:SGTC_669|Compound:0108-0021|FD-Score:3.64|P-value:2.11E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.26|P-value:8.11E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:3.45|P-value:4.14E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:3.91|P-value:7.60E-5 ID:SGTC_934|Compound:2950-0684|FD-Score:-4.68|P-value:2.01E-6 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.73|P-value:1.19E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.15|P-value:9.25E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.51|P-value:3.33E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.28|P-value:1.65E-5 ID:SGTC_1377|Compound:2944-0135|FD-Score:-3.21|P-value:7.55E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.59|P-value:2.57E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.60|P-value:2.44E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.59|P-value:2.00E-4 ID:SGTC_1903|Compound:st060211|FD-Score:-3.44|P-value:3.47E-4 ID:SGTC_1911|Compound:st060208|FD-Score:4.57|P-value:4.56E-6 ID:SGTC_1940|Compound:st075990|FD-Score:3.21|P-value:9.63E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.31|P-value:6.83E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.26|P-value:8.03E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-3.66|P-value:1.53E-4 ID:SGTC_2397|Compound:5161689|FD-Score:4.09|P-value:3.59E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.54|P-value:3.04E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.95|P-value:4.87E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:3.24|P-value:8.49E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.26|P-value:8.11E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-3.60|P-value:1.90E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.93|P-value:6.88E-5 ID:SGTC_3136|Compound:9095672|FD-Score:3.70|P-value:1.66E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.87|P-value:8.89E-5 ID:SGTC_3143|Compound:9086658|FD-Score:6.54|P-value:9.69E-11 ID:SGTC_3217|Compound:9129673|FD-Score:3.41|P-value:4.72E-4 ID:SGTC_3245|Compound:9134821|FD-Score:4.00|P-value:5.25E-5 ID:SGTC_3321|Compound:9137793|FD-Score:3.50|P-value:3.47E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.50|P-value:3.47E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.55|P-value:2.90E-4 ID:SGTC_173|Compound:1273-0061|FD-Score:3.37|P-value:5.59E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:3.70|P-value:1.68E-4 ID:SGTC_415|Compound:dibenzyl ketone|FD-Score:-3.35|P-value:4.72E-4 ID:SGTC_443|Compound:clozapine|FD-Score:3.25|P-value:8.20E-4 ID:SGTC_525|Compound:k781-2675|FD-Score:4.99|P-value:6.39E-7 ID:SGTC_669|Compound:0108-0021|FD-Score:3.64|P-value:2.11E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.26|P-value:8.11E-4 ID:SGTC_928|Compound:2764-0428|FD-Score:3.45|P-value:4.14E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:3.91|P-value:7.60E-5 ID:SGTC_934|Compound:2950-0684|FD-Score:-4.68|P-value:2.01E-6 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.73|P-value:1.19E-4 ID:SGTC_1022|Compound:4477-0945|FD-Score:-3.15|P-value:9.25E-4 ID:SGTC_1074|Compound:carmofur|FD-Score:3.51|P-value:3.33E-4 ID:SGTC_1286|Compound:0929-0070|FD-Score:4.28|P-value:1.65E-5 ID:SGTC_1377|Compound:2944-0135|FD-Score:-3.21|P-value:7.55E-4 ID:SGTC_1628|Compound:st004252|FD-Score:3.59|P-value:2.57E-4 ID:SGTC_1712|Compound:st032288|FD-Score:3.60|P-value:2.44E-4 ID:SGTC_1894|Compound:3',6'-dihydroxyflavone|FD-Score:-3.59|P-value:2.00E-4 ID:SGTC_1903|Compound:st060211|FD-Score:-3.44|P-value:3.47E-4 ID:SGTC_1911|Compound:st060208|FD-Score:4.57|P-value:4.56E-6 ID:SGTC_1940|Compound:st075990|FD-Score:3.21|P-value:9.63E-4 ID:SGTC_1942|Compound:st076330|FD-Score:3.31|P-value:6.83E-4 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:3.26|P-value:8.03E-4 ID:SGTC_1984|Compound:st072354|FD-Score:-3.66|P-value:1.53E-4 ID:SGTC_2397|Compound:5161689|FD-Score:4.09|P-value:3.59E-5 ID:SGTC_2418|Compound:pentamidine|FD-Score:3.54|P-value:3.04E-4 ID:SGTC_2754|Compound:granisetron|FD-Score:-3.95|P-value:4.87E-5 ID:SGTC_2755|Compound:lomerizine|FD-Score:3.24|P-value:8.49E-4 ID:SGTC_2842|Compound:9000675|FD-Score:3.26|P-value:8.11E-4 ID:SGTC_2946|Compound:9065502|FD-Score:-3.60|P-value:1.90E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.93|P-value:6.88E-5 ID:SGTC_3136|Compound:9095672|FD-Score:3.70|P-value:1.66E-4 ID:SGTC_3137|Compound:9097352|FD-Score:3.87|P-value:8.89E-5 ID:SGTC_3143|Compound:9086658|FD-Score:6.54|P-value:9.69E-11 ID:SGTC_3217|Compound:9129673|FD-Score:3.41|P-value:4.72E-4 ID:SGTC_3245|Compound:9134821|FD-Score:4.00|P-value:5.25E-5 ID:SGTC_3321|Compound:9137793|FD-Score:3.50|P-value:3.47E-4 ID:SGTC_3330|Compound:9141424|FD-Score:3.50|P-value:3.47E-4 ID:SGTC_3350|Compound:9150499|FD-Score:3.55|P-value:2.90E-4

Top fitness defect scores for YGR186W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_3143 9086658 6.54 49.5 µM 9.69E-11 3 2.98E-11 0.78
2 SGTC_525 k781-2675 4.99 26.7 µM 6.39E-7 redox potentiating 32 3.07E-7 0.15
3 SGTC_1911 st060208 4.57 74.3 µM 4.56E-6 6 2.42E-6 0.41
4 SGTC_1286 0929-0070 4.28 131.0 µM 1.65E-5 3 9.38E-6 0.05
5 SGTC_2397 5161689 4.09 200.0 µM 3.59E-5 5 2.12E-5 0.14
6 SGTC_3245 9134821 4.00 49.5 µM 5.25E-5 2 3.16E-5 0.16
7 SGTC_3126 9127922 3.93 49.5 µM 6.88E-5 fatty acid desaturase (OLE1) 31 4.21E-5 0.05
8 SGTC_930 2810-3379 3.91 265.0 µM 7.60E-5 4 4.67E-5 0.04
9 SGTC_3137 9097352 3.87 49.5 µM 8.89E-5 7 5.51E-5 0.28
10 SGTC_3136 9095672 3.70 49.5 µM 1.66E-4 6 1.06E-4 0.02
11 SGTC_178 4092-0806 3.70 87.5 µM 1.68E-4 ergosterol depletion effects on membrane 21 1.07E-4 0.08
12 SGTC_669 0108-0021 3.64 26.2 µM 2.11E-4 fatty acid desaturase (OLE1) 17 1.37E-4 0.27
13 SGTC_1712 st032288 3.60 53.4 µM 2.44E-4 calcium & mitochondrial duress 41 1.60E-4 0.03
14 SGTC_1628 st004252 3.59 77.7 µM 2.57E-4 5 1.68E-4 0.02
15 SGTC_3350 9150499 3.55 3.6 µM 2.90E-4 cell wall 18 1.91E-4 0.05
16 SGTC_2418 pentamidine 3.54 31.7 µM 3.04E-4 mitochondrial processes 21 2.01E-4 0.13
17 SGTC_1074 carmofur 3.51 3.8 µM 3.33E-4 exosome 36 2.21E-4 0.08
18 SGTC_3321 9137793 3.50 26.8 µM 3.47E-4 cell wall 21 2.31E-4 0.13
19 SGTC_3330 9141424 3.50 71.4 µM 3.47E-4 amide catabolism 5 2.31E-4 0.01
20 SGTC_928 2764-0428 3.45 58.7 µM 4.14E-4 cell wall 28 2.77E-4 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.158 2.66E-20 YKL021C MAK11 het Protein involved in an early, nucleolar step of 60S ribosomal subunit biogenesis; essential for cell growth and replication of killer M1 dsRNA virus; contains four beta-transducin repeats
0.103 2.23E-9 YKR018C YKR018C hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus; protein abundance increases in response to DNA replication stress; YKR018C has a paralog, IML2, that arose from the whole genome duplication
0.101 3.96E-9 YPR015C_p YPR015C_p hom Putative protein of unknown function; overexpression causes a cell cycle delay or arrest
0.098 1.21E-8 YEL061C CIN8 hom Kinesin motor protein involved in mitotic spindle assembly and chromosome segregation
0.098 1.45E-8 YDL167C NRP1 hom Putative RNA binding protein of unknown function; localizes to stress granules induced by glucose deprivation; predicted to be involved in ribosome biogenesis
0.095 3.80E-8 YDR368W YPR1 hom NADPH-dependent aldo-keto reductase; reduces multiple substrates including 2-methylbutyraldehyde and D,L-glyceraldehyde, expression is induced by osmotic and oxidative stress; functionally redundant with other aldo-keto reductases; protein abundance increases in response to DNA replication stress; YPR1 has a paralog, GCY1, that arose from the whole genome duplication
0.090 1.68E-7 YDR361C BCP1 het Essential protein involved in nuclear export of Mss4p, which is a lipid kinase that generates phosphatidylinositol 4,5-biphosphate and plays a role in actin cytoskeleton organization and vesicular transport
0.089 2.37E-7 YMR244C-A COA6 hom Protein involved in cytochrome c oxidase assembly; also required for efficient formation of respiratory supercomplexes comprised of Complexes III and IV; localizes to the mitochondrial intermembrane space; has a human homolog; transcription is induced in response to the DNA-damaging agent MMS; protein abundance increases in response to DNA replication stress
0.086 6.34E-7 YDR407C TRS120 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; involved in endoplasmic reticulum (ER) to Golgi membrane traffic
0.083 1.37E-6 YML128C MSC1 hom Protein of unknown function; mutant is defective in directing meiotic recombination events to homologous chromatids; the authentic, non-tagged protein is detected in highly purified mitochondria and is phosphorylated
0.082 1.81E-6 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.081 2.34E-6 YMR273C ZDS1 hom Protein with a role in regulating Swe1p-dependent polarized growth; involved in maintaining Cdc55p in the cytoplasm where it promotes mitotic entry; involved in mitotic exit through Cdc14p regulation; interacts with silencing proteins at telomeres; has a role in Bcy1p localization; implicated in mRNA nuclear export; ZDS1 has a paralog, ZDS2, that arose from the whole genome duplication
0.081 2.56E-6 YMR001C-A_p YMR001C-A_p hom Putative protein of unknown function
0.079 4.99E-6 YMR043W MCM1 het Transcription factor involved in cell-type-specific transcription and pheromone response; plays a central role in the formation of both repressor and activator complexes
0.078 6.40E-6 YDL196W_d YDL196W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31