YGR206W / MVB12

ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR206W / MVB12

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-10.20|P-value:3.44E-25 ID:SGTC_59|Compound:0330-0160|FD-Score:-4.10|P-value:1.76E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:-3.20|P-value:6.43E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.08|P-value:8.15E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:5.15|P-value:7.63E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.71|P-value:7.73E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:4.62|P-value:1.24E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.27|P-value:4.22E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:5.33|P-value:2.80E-8 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.14|P-value:7.95E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.58|P-value:1.32E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:5.09|P-value:1.11E-7 ID:SGTC_379|Compound:3970-0972|FD-Score:3.11|P-value:7.39E-4 ID:SGTC_393|Compound:acivicin|FD-Score:4.63|P-value:1.21E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.39|P-value:3.91E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:3.79|P-value:5.63E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.66|P-value:9.27E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:-4.05|P-value:2.16E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.86|P-value:4.11E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:-3.70|P-value:9.47E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:3.04|P-value:9.42E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.42|P-value:2.37E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.41|P-value:2.53E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:5.13|P-value:8.78E-8 ID:SGTC_1093|Compound:fluphenazine|FD-Score:-3.41|P-value:2.98E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.52|P-value:1.65E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.08|P-value:8.39E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:7.41|P-value:2.33E-14 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.29|P-value:3.87E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.52|P-value:1.64E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-3.07|P-value:9.93E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.30|P-value:3.73E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:7.26|P-value:7.87E-14 ID:SGTC_1642|Compound:st009582|FD-Score:4.41|P-value:3.47E-6 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.38|P-value:4.01E-6 ID:SGTC_1800|Compound:st050079|FD-Score:3.13|P-value:6.84E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.06|P-value:1.73E-5 ID:SGTC_1814|Compound:st052110|FD-Score:-3.72|P-value:8.70E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.09|P-value:8.06E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.15|P-value:1.15E-5 ID:SGTC_1902|Compound:st060212|FD-Score:-3.19|P-value:6.67E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.17|P-value:6.04E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.29|P-value:3.92E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.26|P-value:4.33E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-3.63|P-value:1.28E-4 ID:SGTC_2021|Compound:4019055|FD-Score:10.40|P-value:1.82E-26 ID:SGTC_2138|Compound:5575593|FD-Score:-3.19|P-value:6.53E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.20|P-value:5.42E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.15|P-value:1.17E-5 ID:SGTC_2438|Compound:5259590|FD-Score:-3.34|P-value:3.83E-4 ID:SGTC_2442|Compound:5158906|FD-Score:-3.37|P-value:3.37E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.15|P-value:6.44E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.29|P-value:3.95E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.39|P-value:3.81E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.56|P-value:1.21E-11 ID:SGTC_2701|Compound:st077767|FD-Score:-3.86|P-value:4.91E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.26|P-value:6.94E-6 ID:SGTC_2794|Compound:7785382|FD-Score:3.39|P-value:2.73E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.96|P-value:2.68E-5 ID:SGTC_3021|Compound:9087658|FD-Score:3.26|P-value:4.40E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.55|P-value:1.47E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.80|P-value:4.95E-7 ID:SGTC_58|Compound:0957-0393|FD-Score:-10.20|P-value:3.44E-25 ID:SGTC_59|Compound:0330-0160|FD-Score:-4.10|P-value:1.76E-5 ID:SGTC_87|Compound:0100-0052|FD-Score:-3.20|P-value:6.43E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.08|P-value:8.15E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:5.15|P-value:7.63E-8 ID:SGTC_168|Compound:3970-0862|FD-Score:3.71|P-value:7.73E-5 ID:SGTC_212|Compound:3807-4682|FD-Score:4.62|P-value:1.24E-6 ID:SGTC_214|Compound:4373-3496|FD-Score:3.27|P-value:4.22E-4 ID:SGTC_255|Compound:tunicamycin|FD-Score:5.33|P-value:2.80E-8 ID:SGTC_309|Compound:k408-0051|FD-Score:-3.14|P-value:7.95E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:3.58|P-value:1.32E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:5.09|P-value:1.11E-7 ID:SGTC_379|Compound:3970-0972|FD-Score:3.11|P-value:7.39E-4 ID:SGTC_393|Compound:acivicin|FD-Score:4.63|P-value:1.21E-6 ID:SGTC_514|Compound:4466-0038|FD-Score:4.39|P-value:3.91E-6 ID:SGTC_518|Compound:1021-0119|FD-Score:3.79|P-value:5.63E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:3.66|P-value:9.27E-5 ID:SGTC_667|Compound:1498-1044|FD-Score:-4.05|P-value:2.16E-5 ID:SGTC_671|Compound:0560-0003|FD-Score:3.86|P-value:4.11E-5 ID:SGTC_821|Compound:1114-0266|FD-Score:-3.70|P-value:9.47E-5 ID:SGTC_916|Compound:4466-0038|FD-Score:3.04|P-value:9.42E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.42|P-value:2.37E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.41|P-value:2.53E-4 ID:SGTC_978|Compound:1416-0512|FD-Score:5.13|P-value:8.78E-8 ID:SGTC_1093|Compound:fluphenazine|FD-Score:-3.41|P-value:2.98E-4 ID:SGTC_1110|Compound:3-hydroxy-3'-methoxyflavone|FD-Score:3.52|P-value:1.65E-4 ID:SGTC_1113|Compound:artemisinin|FD-Score:3.08|P-value:8.39E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:7.41|P-value:2.33E-14 ID:SGTC_1220|Compound:1838-0075|FD-Score:3.29|P-value:3.87E-4 ID:SGTC_1230|Compound:0170-0365|FD-Score:3.52|P-value:1.64E-4 ID:SGTC_1331|Compound:1472-0049|FD-Score:-3.07|P-value:9.93E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.30|P-value:3.73E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:7.26|P-value:7.87E-14 ID:SGTC_1642|Compound:st009582|FD-Score:4.41|P-value:3.47E-6 ID:SGTC_1789|Compound:tunicamycin|FD-Score:4.38|P-value:4.01E-6 ID:SGTC_1800|Compound:st050079|FD-Score:3.13|P-value:6.84E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.06|P-value:1.73E-5 ID:SGTC_1814|Compound:st052110|FD-Score:-3.72|P-value:8.70E-5 ID:SGTC_1820|Compound:st053765|FD-Score:3.09|P-value:8.06E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:4.15|P-value:1.15E-5 ID:SGTC_1902|Compound:st060212|FD-Score:-3.19|P-value:6.67E-4 ID:SGTC_1908|Compound:st060353|FD-Score:3.17|P-value:6.04E-4 ID:SGTC_1989|Compound:st071868|FD-Score:3.29|P-value:3.92E-4 ID:SGTC_1997|Compound:st073024|FD-Score:3.26|P-value:4.33E-4 ID:SGTC_2017|Compound:5132927|FD-Score:-3.63|P-value:1.28E-4 ID:SGTC_2021|Compound:4019055|FD-Score:10.40|P-value:1.82E-26 ID:SGTC_2138|Compound:5575593|FD-Score:-3.19|P-value:6.53E-4 ID:SGTC_2249|Compound:haloperidol|FD-Score:3.20|P-value:5.42E-4 ID:SGTC_2322|Compound:7413319|FD-Score:4.15|P-value:1.17E-5 ID:SGTC_2438|Compound:5259590|FD-Score:-3.34|P-value:3.83E-4 ID:SGTC_2442|Compound:5158906|FD-Score:-3.37|P-value:3.37E-4 ID:SGTC_2481|Compound:5478948|FD-Score:3.15|P-value:6.44E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:3.29|P-value:3.95E-4 ID:SGTC_2619|Compound:obliquin|FD-Score:4.39|P-value:3.81E-6 ID:SGTC_2688|Compound:kanamycin b|FD-Score:6.56|P-value:1.21E-11 ID:SGTC_2701|Compound:st077767|FD-Score:-3.86|P-value:4.91E-5 ID:SGTC_2753|Compound:fluvastatin|FD-Score:4.26|P-value:6.94E-6 ID:SGTC_2794|Compound:7785382|FD-Score:3.39|P-value:2.73E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.96|P-value:2.68E-5 ID:SGTC_3021|Compound:9087658|FD-Score:3.26|P-value:4.40E-4 ID:SGTC_3162|Compound:9099653|FD-Score:3.55|P-value:1.47E-4 ID:SGTC_3308|Compound:9128332|FD-Score:4.80|P-value:4.95E-7

Top fitness defect scores for YGR206W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2021 4019055 10.40 173.0 µM 1.82E-26 endomembrane recycling 8 1.09E-25
2 SGTC_1203 3992-3522 7.41 103.0 µM 2.33E-14 4 6.12E-14
3 SGTC_1495 4469-0029 7.26 237.0 µM 7.87E-14 35 1.99E-13
4 SGTC_2688 kanamycin b 6.56 96.7 µM 1.21E-11 calcium & mitochondrial duress 124 2.62E-11
5 SGTC_255 tunicamycin 5.33 200.5 nM 2.80E-8 unfolded protein response 39 4.82E-8
6 SGTC_159 3970-0959 5.15 50.0 µM 7.63E-8 37 1.27E-7
7 SGTC_978 1416-0512 5.13 87.4 µM 8.78E-8 redox potentiating 11 1.46E-7
8 SGTC_372 0155-0182 5.09 15.9 µM 1.11E-7 mitochondrial response to ROS 13 1.82E-7
9 SGTC_3308 9128332 4.80 17.6 µM 4.95E-7 plasma membrane duress 51 7.79E-7
10 SGTC_393 acivicin 4.63 6.2 µM 1.21E-6 15 1.85E-6
11 SGTC_212 3807-4682 4.62 38.8 µM 1.24E-6 tubulin folding & SWR complex 44 1.90E-6
12 SGTC_1642 st009582 4.41 77.7 µM 3.47E-6 RPP1 & pyrimidine depletion 9 5.14E-6
13 SGTC_2619 obliquin 4.39 87.9 µM 3.81E-6 cell wall 124 5.63E-6
14 SGTC_514 4466-0038 4.39 2.6 nM 3.91E-6 cell wall 129 5.78E-6
15 SGTC_1789 tunicamycin 4.38 25.0 nM 4.01E-6 unfolded protein response 36 5.91E-6
16 SGTC_2753 fluvastatin 4.26 17.1 µM 6.94E-6 azole & statin 25 1.01E-5
17 SGTC_1844 rapamycin 4.15 4.0 nM 1.15E-5 147 1.64E-5
18 SGTC_2322 7413319 4.15 38.2 µM 1.17E-5 ERAD & cell cycle 142 1.67E-5
19 SGTC_1803 st050068 4.06 52.4 µM 1.73E-5 NEO1-PIK1 171 2.44E-5
20 SGTC_2937 9044753 3.96 11.3 µM 2.68E-5 60S ribosome export 31 3.73E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.297 4.29E-69 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.272 5.22E-58 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.263 4.47E-54 YLR119W SRN2 hom Component of the ESCRT-I complex, which is involved in ubiquitin-dependent sorting of proteins into the endosome; suppressor of rna1-1 mutation; may be involved in RNA export from nucleus
0.253 3.87E-50 YDR486C VPS60 hom Cytoplasmic and vacuolar membrane protein involved in late endosome to vacuole transport; required for normal filament maturation during pseudohyphal growth; may function in targeting cargo proteins for degradation; interacts with Vta1p
0.238 1.44E-44 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.213 7.59E-36 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.213 1.07E-35 YNL294C RIM21 hom pH sensor molecule, component of the RIM101 pathway; has a role in cell wall construction and alkaline pH response; is glycosylated and phosphorylated; interacts with Dfg16p and Rim9p to form a pH-sensing complex; localization to the plasma membrane is dependent on Dfg16p and Rim9p; has similarity to A. nidulans PalH
0.209 1.90E-34 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.207 1.04E-33 YJL094C KHA1 hom Putative K+/H+ antiporter with a probable role in intracellular cation homeostasis, localized to Golgi vesicles and detected in highly purified mitochondria in high-throughput studies
0.207 9.26E-34 YNR005C_d YNR005C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.205 4.31E-33 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.203 1.21E-32 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.198 3.77E-31 YOR089C VPS21 hom Rab family GTPase; required for endocytic transport and for sorting of vacuolar hydrolases; localized in endocytic intermediates; detected in mitochondria; geranylgeranylation required for membrane association; mammalian Rab5 homolog; protein abundance increases in response to DNA replication stress; VPS21 has a paralog, YPT53, that arose from the whole genome duplication
0.197 8.89E-31 YKR035W-A DID2 hom Class E protein of the vacuolar protein-sorting (Vps) pathway; binds Vps4p and directs it to dissociate ESCRT-III complexes; forms a functional and physical complex with Ist1p; human ortholog may be altered in breast tumors
0.193 2.15E-29 YHL002W HSE1 hom Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes