YGR221C / TOS2

Protein involved in localization of Cdc24p to the site of bud growth; may act as a membrane anchor; localizes to the bud neck and bud tip; potentially phosphorylated by Cdc28p

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR221C / TOS2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.72|P-value:1.12E-9 ID:SGTC_138|Compound:0850-0259|FD-Score:5.04|P-value:6.68E-8 ID:SGTC_169|Compound:k015-0017|FD-Score:3.53|P-value:1.14E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:-4.22|P-value:5.07E-6 ID:SGTC_295|Compound:k015-0027|FD-Score:3.15|P-value:4.93E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:5.75|P-value:9.35E-10 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:5.38|P-value:9.24E-9 ID:SGTC_655|Compound:k060-0012|FD-Score:3.66|P-value:6.41E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.09|P-value:6.20E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.27|P-value:3.19E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.16|P-value:4.79E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.07|P-value:6.58E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.00|P-value:8.49E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.35|P-value:2.31E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.17|P-value:4.59E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.04|P-value:1.19E-5 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.04|P-value:7.46E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.01|P-value:8.22E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.21|P-value:4.01E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.42|P-value:1.74E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:6.11|P-value:8.57E-11 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:4.48|P-value:1.44E-6 ID:SGTC_1689|Compound:st020265|FD-Score:3.92|P-value:2.05E-5 ID:SGTC_1696|Compound:st025591|FD-Score:3.10|P-value:6.04E-4 ID:SGTC_1708|Compound:st033234|FD-Score:-3.20|P-value:4.16E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.36|P-value:2.20E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.06|P-value:1.09E-5 ID:SGTC_1791|Compound:st048313|FD-Score:3.01|P-value:8.29E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.00|P-value:8.54E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.87|P-value:2.66E-5 ID:SGTC_2308|Compound:7584454|FD-Score:3.83|P-value:3.15E-5 ID:SGTC_2314|Compound:7727897|FD-Score:3.04|P-value:7.36E-4 ID:SGTC_2319|Compound:6033012|FD-Score:4.33|P-value:3.02E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-7.27|P-value:1.55E-14 ID:SGTC_2324|Compound:6149169|FD-Score:-3.81|P-value:3.46E-5 ID:SGTC_2385|Compound:9074692|FD-Score:3.84|P-value:3.01E-5 ID:SGTC_2387|Compound:9077560|FD-Score:3.01|P-value:8.40E-4 ID:SGTC_2395|Compound:5646831|FD-Score:-3.57|P-value:9.39E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.38|P-value:2.05E-4 ID:SGTC_2475|Compound:5812728|FD-Score:3.60|P-value:8.23E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.06|P-value:1.10E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.38|P-value:2.06E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.59|P-value:8.86E-5 ID:SGTC_2788|Compound:5282734|FD-Score:4.42|P-value:1.88E-6 ID:SGTC_2809|Compound:7964133|FD-Score:3.04|P-value:7.50E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.02|P-value:8.03E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.14|P-value:5.17E-4 ID:SGTC_2844|Compound:9010103|FD-Score:4.22|P-value:5.18E-6 ID:SGTC_2849|Compound:9019321|FD-Score:3.32|P-value:2.60E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.12|P-value:5.49E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.30|P-value:2.82E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.14|P-value:5.17E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.03|P-value:7.66E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.99|P-value:1.55E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.20|P-value:4.08E-4 ID:SGTC_3000|Compound:9075062|FD-Score:3.95|P-value:1.83E-5 ID:SGTC_3213|Compound:9129884|FD-Score:3.48|P-value:1.38E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.30|P-value:3.54E-6 ID:SGTC_3283|Compound:9109332|FD-Score:-3.31|P-value:2.68E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.22|P-value:3.82E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.79|P-value:3.67E-5 ID:SGTC_3328|Compound:9140624|FD-Score:6.56|P-value:3.59E-12 ID:SGTC_58|Compound:0957-0393|FD-Score:5.72|P-value:1.12E-9 ID:SGTC_138|Compound:0850-0259|FD-Score:5.04|P-value:6.68E-8 ID:SGTC_169|Compound:k015-0017|FD-Score:3.53|P-value:1.14E-4 ID:SGTC_272|Compound:doxorubicin|FD-Score:-4.22|P-value:5.07E-6 ID:SGTC_295|Compound:k015-0027|FD-Score:3.15|P-value:4.93E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:5.75|P-value:9.35E-10 ID:SGTC_423|Compound:5-fluorouracil|FD-Score:5.38|P-value:9.24E-9 ID:SGTC_655|Compound:k060-0012|FD-Score:3.66|P-value:6.41E-5 ID:SGTC_751|Compound:0833-0568|FD-Score:3.09|P-value:6.20E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:-3.27|P-value:3.19E-4 ID:SGTC_869|Compound:0564-0080|FD-Score:3.16|P-value:4.79E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:-3.07|P-value:6.58E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:-3.00|P-value:8.49E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:3.35|P-value:2.31E-4 ID:SGTC_1094|Compound:duloxetine|FD-Score:3.17|P-value:4.59E-4 ID:SGTC_1121|Compound:fusaric acid|FD-Score:4.04|P-value:1.19E-5 ID:SGTC_1295|Compound:1013-0063|FD-Score:3.04|P-value:7.46E-4 ID:SGTC_1347|Compound:1493-0251|FD-Score:3.01|P-value:8.22E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:3.21|P-value:4.01E-4 ID:SGTC_1400|Compound:3771-8998|FD-Score:3.42|P-value:1.74E-4 ID:SGTC_1601|Compound:4'-hydroxyflavanone|FD-Score:6.11|P-value:8.57E-11 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:4.48|P-value:1.44E-6 ID:SGTC_1689|Compound:st020265|FD-Score:3.92|P-value:2.05E-5 ID:SGTC_1696|Compound:st025591|FD-Score:3.10|P-value:6.04E-4 ID:SGTC_1708|Compound:st033234|FD-Score:-3.20|P-value:4.16E-4 ID:SGTC_1740|Compound:st038286|FD-Score:3.36|P-value:2.20E-4 ID:SGTC_1746|Compound:st038117|FD-Score:4.06|P-value:1.09E-5 ID:SGTC_1791|Compound:st048313|FD-Score:3.01|P-value:8.29E-4 ID:SGTC_1945|Compound:st075991|FD-Score:-3.00|P-value:8.54E-4 ID:SGTC_2133|Compound:5320837|FD-Score:3.87|P-value:2.66E-5 ID:SGTC_2308|Compound:7584454|FD-Score:3.83|P-value:3.15E-5 ID:SGTC_2314|Compound:7727897|FD-Score:3.04|P-value:7.36E-4 ID:SGTC_2319|Compound:6033012|FD-Score:4.33|P-value:3.02E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-7.27|P-value:1.55E-14 ID:SGTC_2324|Compound:6149169|FD-Score:-3.81|P-value:3.46E-5 ID:SGTC_2385|Compound:9074692|FD-Score:3.84|P-value:3.01E-5 ID:SGTC_2387|Compound:9077560|FD-Score:3.01|P-value:8.40E-4 ID:SGTC_2395|Compound:5646831|FD-Score:-3.57|P-value:9.39E-5 ID:SGTC_2465|Compound:5809242|FD-Score:3.38|P-value:2.05E-4 ID:SGTC_2475|Compound:5812728|FD-Score:3.60|P-value:8.23E-5 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.06|P-value:1.10E-5 ID:SGTC_2591|Compound:skatole|FD-Score:3.38|P-value:2.06E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.59|P-value:8.86E-5 ID:SGTC_2788|Compound:5282734|FD-Score:4.42|P-value:1.88E-6 ID:SGTC_2809|Compound:7964133|FD-Score:3.04|P-value:7.50E-4 ID:SGTC_2810|Compound:7959871|FD-Score:3.02|P-value:8.03E-4 ID:SGTC_2815|Compound:7968870|FD-Score:3.14|P-value:5.17E-4 ID:SGTC_2844|Compound:9010103|FD-Score:4.22|P-value:5.18E-6 ID:SGTC_2849|Compound:9019321|FD-Score:3.32|P-value:2.60E-4 ID:SGTC_2912|Compound:7964298|FD-Score:3.12|P-value:5.49E-4 ID:SGTC_2913|Compound:7966556|FD-Score:3.30|P-value:2.82E-4 ID:SGTC_2944|Compound:9067346|FD-Score:-3.14|P-value:5.17E-4 ID:SGTC_2952|Compound:9073731|FD-Score:3.03|P-value:7.66E-4 ID:SGTC_2956|Compound:9076196|FD-Score:3.99|P-value:1.55E-5 ID:SGTC_2975|Compound:9084847|FD-Score:3.20|P-value:4.08E-4 ID:SGTC_3000|Compound:9075062|FD-Score:3.95|P-value:1.83E-5 ID:SGTC_3213|Compound:9129884|FD-Score:3.48|P-value:1.38E-4 ID:SGTC_3221|Compound:9128209|FD-Score:4.30|P-value:3.54E-6 ID:SGTC_3283|Compound:9109332|FD-Score:-3.31|P-value:2.68E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.22|P-value:3.82E-4 ID:SGTC_3326|Compound:9140064|FD-Score:-3.79|P-value:3.67E-5 ID:SGTC_3328|Compound:9140624|FD-Score:6.56|P-value:3.59E-12

Top fitness defect scores for YGR221C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3328 9140624 6.56 29.0 µM 3.59E-12 2 2.75E-11
2 SGTC_1601 4'-hydroxyflavanone 6.11 55.5 µM 8.57E-11 RPP1 & pyrimidine depletion 10 5.04E-10
3 SGTC_304 1310-0298 5.75 97.0 µM 9.35E-10 cell wall 22 4.51E-9
4 SGTC_58 0957-0393 5.72 9.0 µM 1.12E-9 cell wall 228 5.31E-9
5 SGTC_423 5-fluorouracil 5.38 457.0 µM 9.24E-9 exosome 4 3.69E-8
6 SGTC_138 0850-0259 5.04 40.9 µM 6.68E-8 cell wall 144 2.27E-7
7 SGTC_1680 diphenhydramine 4.48 100.0 µM 1.44E-6 NEO1 18 3.81E-6
8 SGTC_2788 5282734 4.42 71.4 µM 1.88E-6 28 4.86E-6
9 SGTC_2319 6033012 4.33 152.6 µM 3.02E-6 tubulin folding & SWR complex 26 7.50E-6
10 SGTC_3221 9128209 4.30 49.5 µM 3.54E-6 8 8.68E-6
11 SGTC_2844 9010103 4.22 26.0 µM 5.18E-6 25 1.23E-5
12 SGTC_1746 st038117 4.06 42.0 µM 1.09E-5 29 2.43E-5
13 SGTC_2582 agaric acid 4.06 100.0 µM 1.10E-5 redox potentiating 302 2.46E-5
14 SGTC_1121 fusaric acid 4.04 630.5 nM 1.19E-5 30 2.64E-5
15 SGTC_2956 9076196 3.99 2.5 µM 1.55E-5 14 3.37E-5
16 SGTC_3000 9075062 3.95 71.4 µM 1.83E-5 18 3.92E-5
17 SGTC_1689 st020265 3.92 53.5 µM 2.05E-5 16 4.36E-5
18 SGTC_2133 5320837 3.87 200.0 µM 2.66E-5 ERG2 25 5.53E-5
19 SGTC_2385 9074692 3.84 200.0 µM 3.01E-5 45 6.21E-5
20 SGTC_2308 7584454 3.83 197.6 µM 3.15E-5 20 6.47E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.221 3.01E-38 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.193 1.91E-29 YJR142W_p YJR142W_p hom Putative protein of unknown function; GST fusion protein is a Dbf2p-Mob1p phosphorylation target in a proteome chip analysis; synthetic lethal with PH085 deletion; plays a role in restricting Ty1 transposition; nudix hydrolase family member
0.187 7.02E-28 YKL076C_d PSY1_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 69% of ORF overlaps the uncharacterized ORF YKL075C
0.186 1.55E-27 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.186 1.59E-27 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.176 7.18E-25 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.172 9.11E-24 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.171 2.11E-23 YML117W NAB6 hom Putative RNA-binding protein that associates with mRNAs encoding cell wall proteins in high-throughput studies; deletion mutants display increased sensitivity to some cell wall disrupting agents; expression negatively regulated by cAMP
0.171 2.50E-23 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.168 1.32E-22 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.167 2.34E-22 YDL203C ACK1 hom Protein that functions upstream of Pkc1p in the cell wall integrity pathway; GFP-fusion protein expression is induced in response to the DNA-damaging agent MMS; non-tagged Ack1p is detected in purified mitochondria
0.167 2.64E-22 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.165 5.53E-22 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.165 9.50E-22 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.161 7.98E-21 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication