YGR225W / AMA1

Activator of meiotic anaphase promoting complex (APC/C); Cdc20p family member; required for initiation of spore wall assembly; required for Clb1p degradation during meiosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR225W / AMA1

Click on Significant Values for Screen Details ID:SGTC_39|Compound:0986-0283|FD-Score:-3.63|P-value:8.88E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.23|P-value:6.47E-6 ID:SGTC_316|Compound:k060-0003|FD-Score:3.98|P-value:2.01E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.28|P-value:3.46E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:3.71|P-value:6.68E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:3.85|P-value:3.69E-5 ID:SGTC_443|Compound:clozapine|FD-Score:-3.01|P-value:9.46E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.57|P-value:1.15E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.22|P-value:4.45E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:3.03|P-value:8.84E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.10|P-value:6.90E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.13|P-value:6.25E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.46|P-value:2.01E-6 ID:SGTC_1251|Compound:0490-5699|FD-Score:4.29|P-value:4.98E-6 ID:SGTC_1386|Compound:3228-0095|FD-Score:5.09|P-value:7.84E-8 ID:SGTC_1397|Compound:3532-0190|FD-Score:-3.46|P-value:1.75E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:5.00|P-value:1.27E-7 ID:SGTC_1558|Compound:st069300|FD-Score:-3.74|P-value:5.59E-5 ID:SGTC_1740|Compound:st038286|FD-Score:4.03|P-value:1.61E-5 ID:SGTC_1840|Compound:st055363|FD-Score:3.96|P-value:2.20E-5 ID:SGTC_1917|Compound:st066507|FD-Score:3.05|P-value:8.25E-4 ID:SGTC_2396|Compound:5342950|FD-Score:-3.30|P-value:3.24E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.38|P-value:2.43E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.30|P-value:3.38E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.54|P-value:1.34E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.23|P-value:6.45E-6 ID:SGTC_2785|Compound:7756463|FD-Score:-4.40|P-value:2.70E-6 ID:SGTC_2787|Compound:5284540|FD-Score:4.06|P-value:1.39E-5 ID:SGTC_2892|Compound:9047358|FD-Score:3.33|P-value:3.03E-4 ID:SGTC_2919|Compound:7978188|FD-Score:-3.02|P-value:9.06E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.83|P-value:3.97E-5 ID:SGTC_3053|Compound:9095903|FD-Score:3.72|P-value:6.19E-5 ID:SGTC_3285|Compound:9112818|FD-Score:3.11|P-value:6.77E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.10|P-value:7.09E-4 ID:SGTC_39|Compound:0986-0283|FD-Score:-3.63|P-value:8.88E-5 ID:SGTC_269|Compound:exalamide|FD-Score:4.23|P-value:6.47E-6 ID:SGTC_316|Compound:k060-0003|FD-Score:3.98|P-value:2.01E-5 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.28|P-value:3.46E-4 ID:SGTC_348|Compound:k064-0012|FD-Score:3.71|P-value:6.68E-5 ID:SGTC_438|Compound:sphingosine|FD-Score:3.85|P-value:3.69E-5 ID:SGTC_443|Compound:clozapine|FD-Score:-3.01|P-value:9.46E-4 ID:SGTC_526|Compound:4469-0568|FD-Score:3.57|P-value:1.15E-4 ID:SGTC_732|Compound:3970-1727|FD-Score:3.22|P-value:4.45E-4 ID:SGTC_970|Compound:1315-0089|FD-Score:3.03|P-value:8.84E-4 ID:SGTC_1050|Compound:3232-1156|FD-Score:3.10|P-value:6.90E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:3.13|P-value:6.25E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-4.46|P-value:2.01E-6 ID:SGTC_1251|Compound:0490-5699|FD-Score:4.29|P-value:4.98E-6 ID:SGTC_1386|Compound:3228-0095|FD-Score:5.09|P-value:7.84E-8 ID:SGTC_1397|Compound:3532-0190|FD-Score:-3.46|P-value:1.75E-4 ID:SGTC_1496|Compound:4469-0713|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:5.00|P-value:1.27E-7 ID:SGTC_1558|Compound:st069300|FD-Score:-3.74|P-value:5.59E-5 ID:SGTC_1740|Compound:st038286|FD-Score:4.03|P-value:1.61E-5 ID:SGTC_1840|Compound:st055363|FD-Score:3.96|P-value:2.20E-5 ID:SGTC_1917|Compound:st066507|FD-Score:3.05|P-value:8.25E-4 ID:SGTC_2396|Compound:5342950|FD-Score:-3.30|P-value:3.24E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:-3.38|P-value:2.43E-4 ID:SGTC_2541|Compound:muurolladie-3-one|FD-Score:3.30|P-value:3.38E-4 ID:SGTC_2553|Compound:4-methylesculetin|FD-Score:3.54|P-value:1.34E-4 ID:SGTC_2563|Compound:tomatine|FD-Score:4.23|P-value:6.45E-6 ID:SGTC_2785|Compound:7756463|FD-Score:-4.40|P-value:2.70E-6 ID:SGTC_2787|Compound:5284540|FD-Score:4.06|P-value:1.39E-5 ID:SGTC_2892|Compound:9047358|FD-Score:3.33|P-value:3.03E-4 ID:SGTC_2919|Compound:7978188|FD-Score:-3.02|P-value:9.06E-4 ID:SGTC_3006|Compound:9075424|FD-Score:3.83|P-value:3.97E-5 ID:SGTC_3053|Compound:9095903|FD-Score:3.72|P-value:6.19E-5 ID:SGTC_3285|Compound:9112818|FD-Score:3.11|P-value:6.77E-4 ID:SGTC_3335|Compound:9143315|FD-Score:3.10|P-value:7.09E-4

Top fitness defect scores for YGR225W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1386 3228-0095 5.09 33.8 µM 7.84E-8 4 1.83E-7
2 SGTC_1545 lovastatin 5.00 49.4 µM 1.27E-7 7 2.88E-7
3 SGTC_1251 0490-5699 4.29 265.0 µM 4.98E-6 26 9.13E-6
4 SGTC_2563 tomatine 4.23 8.5 µM 6.45E-6 34 1.16E-5
5 SGTC_269 exalamide 4.23 29.0 µM 6.47E-6 ergosterol biosynthesis 17 1.17E-5
6 SGTC_2787 5284540 4.06 71.4 µM 1.39E-5 20 2.40E-5
7 SGTC_1740 st038286 4.03 13.2 µM 1.61E-5 25 2.76E-5
8 SGTC_316 k060-0003 3.98 58.6 µM 2.01E-5 16 3.40E-5
9 SGTC_1840 st055363 3.96 71.0 µM 2.20E-5 10 3.70E-5
10 SGTC_438 sphingosine 3.85 6.7 µM 3.69E-5 24 6.02E-5
11 SGTC_3006 9075424 3.83 71.4 µM 3.97E-5 13 6.46E-5
12 SGTC_3053 9095903 3.72 49.5 µM 6.19E-5 Golgi 5 9.83E-5
13 SGTC_348 k064-0012 3.71 10.7 µM 6.68E-5 60S ribosome export 18 1.06E-4
14 SGTC_526 4469-0568 3.57 170.0 µM 1.15E-4 azole & statin 46 1.77E-4
15 SGTC_2553 4-methylesculetin 3.54 85.1 µM 1.34E-4 36 2.04E-4
16 SGTC_2892 9047358 3.33 58.4 µM 3.03E-4 22 4.39E-4
17 SGTC_1496 4469-0713 3.32 57.4 µM 3.12E-4 39 4.52E-4
18 SGTC_2541 muurolladie-3-one 3.30 83.1 µM 3.38E-4 excess fatty acid 55 4.88E-4
19 SGTC_732 3970-1727 3.22 52.7 µM 4.45E-4 22 6.31E-4
20 SGTC_1105 epirubicin 3.13 18.1 µM 6.25E-4 anthracycline transcription coupled DNA repair 53 8.70E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.148 6.73E-18 YDR294C DPL1 hom Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
0.107 5.34E-10 YDR044W HEM13 het Coproporphyrinogen III oxidase, an oxygen requiring enzyme that catalyzes the sixth step in the heme biosynthetic pathway; transcription is repressed by oxygen and heme (via Rox1p and Hap1p)
0.095 3.52E-8 YDR321W ASP1 hom Cytosolic L-asparaginase, involved in asparagine catabolism; catalyzes hydrolysis of L-asparagine to aspartic acid and ammonia, has an important role in therapy of acute lymphoblastic leukemia; synthesized constitutively
0.094 4.34E-8 YNR032C-A HUB1 hom Ubiquitin-like protein modifier; promotes alternative splicing of SRC1 pre-mRNA; binds non-covalently to the HIND domain of Snu66, may function in modification of Sph1p and Hbt1p, functionally complemented by the human or S. pombe ortholog; mechanism of Hub1p adduct formation not yet clear
0.094 5.43E-8 YDR036C EHD3 hom 3-hydroxyisobutyryl-CoA hydrolase, member of a family of enoyl-CoA hydratase/isomerases; non-tagged protein is detected in highly purified mitochondria in high-throughput studies; phosphorylated; mutation affects fluid-phase endocytosis
0.094 5.64E-8 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.087 3.88E-7 YLR377C FBP1 hom Fructose-1,6-bisphosphatase, key regulatory enzyme in the gluconeogenesis pathway, required for glucose metabolism; undergoes either proteasome-mediated or autophagy-mediated degradation depending on growth conditions; interacts with Vid30p
0.084 1.27E-6 YAL060W BDH1 hom NAD-dependent (R,R)-butanediol dehydrogenase, catalyzes oxidation of (R,R)-2,3-butanediol to (3R)-acetoin, oxidation of meso-butanediol to (3S)-acetoin, and reduction of acetoin; enhances use of 2,3-butanediol as an aerobic carbon source
0.080 3.23E-6 YNL020C ARK1 hom Serine/threonine protein kinase; involved in regulation of the cortical actin cytoskeleton; involved in control of endocytosis; ARK1 has a paralog, PRK1, that arose from the whole genome duplication
0.079 4.42E-6 YLR241W_p YLR241W_p hom Putative protein of unknown function, may be involved in detoxification
0.078 6.48E-6 YNL260C LTO1 het Essential protein that forms a complex with Rli1p and Yae1p; ortholog of human ORAOV1, which is overexpressed in solid tumors; inviability of null mutant under standard conditions is complemented by overexpression of ORAOV1; essential for growth under standard (aerobic) conditions but not under anaerobic conditions; may have a role in protection of ribosomal assembly and function from damage due to reactive oxygen species
0.077 8.90E-6 YDR192C NUP42 hom FG-nucleoporin component of central core of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes directly to nucleocytoplasmic transport and maintenance of the NPC permeability barrier and is involved in gene tethering at the nuclear periphery; interacts with Gle1p
0.077 9.04E-6 YMR107W SPG4 hom Protein required for survival at high temperature during stationary phase; not required for growth on nonfermentable carbon sources
0.076 9.84E-6 YJL020C BBC1 hom Protein possibly involved in assembly of actin patches; interacts with an actin assembly factor Las17p and with the SH3 domains of Type I myosins Myo3p and Myo5p; localized predominantly to cortical actin patches
0.076 1.03E-5 YPL070W MUK1 hom Cytoplasmic protein of unknown function containing a Vps9 domain; computational analysis of large-scale protein-protein interaction data suggests a possible role in transcriptional regulation