YGR227W / DIE2

Dolichyl-phosphoglucose-dependent alpha-1,2 glucosyltransferase of the ER, functions in the pathway that synthesizes the dolichol-linked oligosaccharide precursor for N-linked protein glycosylation, has a role in regulation of ITR1 and INO1

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR227W / DIE2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:-3.41|P-value:3.01E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.65|P-value:1.17E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:3.50|P-value:2.15E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.33|P-value:4.00E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.23|P-value:5.81E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.48|P-value:3.28E-6 ID:SGTC_195|Compound:4234-0266|FD-Score:3.29|P-value:4.61E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.20|P-value:6.30E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.10|P-value:9.10E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.27|P-value:4.93E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.56|P-value:1.69E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.10|P-value:9.06E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.95|P-value:3.54E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.37|P-value:3.41E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.18|P-value:6.82E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.42|P-value:4.34E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:6.19|P-value:2.33E-10 ID:SGTC_593|Compound:1203-0218|FD-Score:3.11|P-value:8.60E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.62|P-value:1.32E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.48|P-value:2.32E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.77|P-value:7.49E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.11|P-value:8.70E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.65|P-value:1.45E-6 ID:SGTC_1101|Compound:ebselen|FD-Score:4.47|P-value:3.34E-6 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.70|P-value:9.69E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.20|P-value:6.31E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.46|P-value:3.47E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.08|P-value:9.78E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.24|P-value:5.54E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.15|P-value:7.59E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.57|P-value:1.62E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.44|P-value:2.68E-4 ID:SGTC_1608|Compound:st001473|FD-Score:3.09|P-value:9.40E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.87|P-value:4.85E-5 ID:SGTC_1614|Compound:st002383|FD-Score:3.10|P-value:8.95E-4 ID:SGTC_1665|Compound:st014639|FD-Score:3.41|P-value:3.02E-4 ID:SGTC_1670|Compound:st016595|FD-Score:3.45|P-value:2.52E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.91|P-value:4.11E-5 ID:SGTC_1822|Compound:st052468|FD-Score:3.80|P-value:6.46E-5 ID:SGTC_2009|Compound:4012297|FD-Score:3.80|P-value:6.60E-5 ID:SGTC_2021|Compound:4019055|FD-Score:4.40|P-value:4.80E-6 ID:SGTC_2028|Compound:5153890|FD-Score:3.12|P-value:8.41E-4 ID:SGTC_2050|Compound:5263056|FD-Score:5.43|P-value:2.35E-8 ID:SGTC_2052|Compound:5265182|FD-Score:5.19|P-value:8.61E-8 ID:SGTC_2057|Compound:5236571|FD-Score:3.14|P-value:7.97E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.18|P-value:6.85E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.38|P-value:3.37E-4 ID:SGTC_2437|Compound:5741531|FD-Score:4.78|P-value:7.25E-7 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.22|P-value:5.87E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.60|P-value:1.46E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.16|P-value:7.21E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.11|P-value:8.62E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-4.50|P-value:2.89E-6 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.28|P-value:4.75E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.36|P-value:3.43E-8 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.97|P-value:3.23E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.50|P-value:2.10E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.40|P-value:3.12E-4 ID:SGTC_2801|Compound:7907800|FD-Score:8.65|P-value:1.47E-18 ID:SGTC_2887|Compound:9044784|FD-Score:3.26|P-value:5.14E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-3.63|P-value:1.27E-4 ID:SGTC_3195|Compound:9113082|FD-Score:3.84|P-value:5.53E-5 ID:SGTC_3196|Compound:9111891|FD-Score:-3.56|P-value:1.70E-4 ID:SGTC_3199|Compound:9111318|FD-Score:3.18|P-value:6.91E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-3.41|P-value:3.01E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.65|P-value:1.17E-4 ID:SGTC_88|Compound:0180-0332|FD-Score:3.50|P-value:2.15E-4 ID:SGTC_98|Compound:0074-0023|FD-Score:3.33|P-value:4.00E-4 ID:SGTC_120|Compound:rhodamine 6G|FD-Score:3.23|P-value:5.81E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:4.48|P-value:3.28E-6 ID:SGTC_195|Compound:4234-0266|FD-Score:3.29|P-value:4.61E-4 ID:SGTC_249|Compound:wiskostatin|FD-Score:3.20|P-value:6.30E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.10|P-value:9.10E-4 ID:SGTC_319|Compound:1348-1596|FD-Score:3.27|P-value:4.93E-4 ID:SGTC_345|Compound:1362-0200|FD-Score:3.56|P-value:1.69E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.10|P-value:9.06E-4 ID:SGTC_493|Compound:zm226600|FD-Score:3.95|P-value:3.54E-5 ID:SGTC_495|Compound:decylubiquinone|FD-Score:3.37|P-value:3.41E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.18|P-value:6.82E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:-4.42|P-value:4.34E-6 ID:SGTC_577|Compound:r071-0003|FD-Score:6.19|P-value:2.33E-10 ID:SGTC_593|Compound:1203-0218|FD-Score:3.11|P-value:8.60E-4 ID:SGTC_679|Compound:0422-0091|FD-Score:3.62|P-value:1.32E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.48|P-value:2.32E-4 ID:SGTC_749|Compound:1000-0129|FD-Score:3.77|P-value:7.49E-5 ID:SGTC_858|Compound:0438-0351|FD-Score:3.11|P-value:8.70E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:4.65|P-value:1.45E-6 ID:SGTC_1101|Compound:ebselen|FD-Score:4.47|P-value:3.34E-6 ID:SGTC_1111|Compound:tnp00062|FD-Score:-3.70|P-value:9.69E-5 ID:SGTC_1289|Compound:0976-0073|FD-Score:3.20|P-value:6.31E-4 ID:SGTC_1357|Compound:1503-1303|FD-Score:4.46|P-value:3.47E-6 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.08|P-value:9.78E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.24|P-value:5.54E-4 ID:SGTC_1528|Compound:arabinosylhypoxanthine|FD-Score:3.15|P-value:7.59E-4 ID:SGTC_1573|Compound:oleylamine|FD-Score:3.57|P-value:1.62E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:-3.44|P-value:2.68E-4 ID:SGTC_1608|Compound:st001473|FD-Score:3.09|P-value:9.40E-4 ID:SGTC_1611|Compound:st001856|FD-Score:3.87|P-value:4.85E-5 ID:SGTC_1614|Compound:st002383|FD-Score:3.10|P-value:8.95E-4 ID:SGTC_1665|Compound:st014639|FD-Score:3.41|P-value:3.02E-4 ID:SGTC_1670|Compound:st016595|FD-Score:3.45|P-value:2.52E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.91|P-value:4.11E-5 ID:SGTC_1822|Compound:st052468|FD-Score:3.80|P-value:6.46E-5 ID:SGTC_2009|Compound:4012297|FD-Score:3.80|P-value:6.60E-5 ID:SGTC_2021|Compound:4019055|FD-Score:4.40|P-value:4.80E-6 ID:SGTC_2028|Compound:5153890|FD-Score:3.12|P-value:8.41E-4 ID:SGTC_2050|Compound:5263056|FD-Score:5.43|P-value:2.35E-8 ID:SGTC_2052|Compound:5265182|FD-Score:5.19|P-value:8.61E-8 ID:SGTC_2057|Compound:5236571|FD-Score:3.14|P-value:7.97E-4 ID:SGTC_2154|Compound:5570129|FD-Score:3.18|P-value:6.85E-4 ID:SGTC_2313|Compound:7726885|FD-Score:-3.38|P-value:3.37E-4 ID:SGTC_2437|Compound:5741531|FD-Score:4.78|P-value:7.25E-7 ID:SGTC_2500|Compound:5-alpha-androstan-3,17-dione|FD-Score:3.22|P-value:5.87E-4 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.60|P-value:1.46E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.16|P-value:7.21E-4 ID:SGTC_2582|Compound:agaric acid|FD-Score:-3.11|P-value:8.62E-4 ID:SGTC_2601|Compound:securinine|FD-Score:-4.50|P-value:2.89E-6 ID:SGTC_2660|Compound:2',4'-dihydroxychalcone|FD-Score:3.28|P-value:4.75E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:5.36|P-value:3.43E-8 ID:SGTC_2753|Compound:fluvastatin|FD-Score:3.97|P-value:3.23E-5 ID:SGTC_2776|Compound:terbinafine|FD-Score:3.50|P-value:2.10E-4 ID:SGTC_2796|Compound:7949634|FD-Score:3.40|P-value:3.12E-4 ID:SGTC_2801|Compound:7907800|FD-Score:8.65|P-value:1.47E-18 ID:SGTC_2887|Compound:9044784|FD-Score:3.26|P-value:5.14E-4 ID:SGTC_3066|Compound:9117245|FD-Score:-3.63|P-value:1.27E-4 ID:SGTC_3195|Compound:9113082|FD-Score:3.84|P-value:5.53E-5 ID:SGTC_3196|Compound:9111891|FD-Score:-3.56|P-value:1.70E-4 ID:SGTC_3199|Compound:9111318|FD-Score:3.18|P-value:6.91E-4

Top fitness defect scores for YGR227W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2801 7907800 8.65 81.8 µM 1.47E-18 1 2.50E-18
2 SGTC_577 r071-0003 6.19 38.6 µM 2.33E-10 fatty acid desaturase (OLE1) 13 3.06E-10
3 SGTC_2050 5263056 5.43 171.0 µM 2.35E-8 fatty acid desaturase (OLE1) 17 2.90E-8
4 SGTC_2688 kanamycin b 5.36 96.7 µM 3.43E-8 calcium & mitochondrial duress 212 4.21E-8
5 SGTC_2052 5265182 5.19 7.1 µM 8.61E-8 7 1.05E-7
6 SGTC_2437 5741531 4.78 144.2 µM 7.25E-7 22 8.56E-7
7 SGTC_964 1287-0107 4.65 19.4 µM 1.45E-6 cell wall 21 1.70E-6
8 SGTC_137 0438-0332 4.48 85.4 µM 3.28E-6 ubiquinone biosynthesis & proteasome 95 3.79E-6
9 SGTC_1101 ebselen 4.47 5.3 µM 3.34E-6 13 3.87E-6
10 SGTC_1357 1503-1303 4.46 39.5 µM 3.47E-6 17 4.01E-6
11 SGTC_2021 4019055 4.40 173.0 µM 4.80E-6 endomembrane recycling 98 5.52E-6
12 SGTC_2753 fluvastatin 3.97 17.1 µM 3.23E-5 azole & statin 37 3.63E-5
13 SGTC_493 zm226600 3.95 134.0 µM 3.54E-5 mitochondrial processes 16 3.97E-5
14 SGTC_1773 st048542 3.91 73.6 µM 4.11E-5 43 4.60E-5
15 SGTC_1611 st001856 3.87 99.4 µM 4.85E-5 20 5.41E-5
16 SGTC_3195 9113082 3.84 49.5 µM 5.53E-5 20 6.16E-5
17 SGTC_1822 st052468 3.80 42.0 µM 6.46E-5 14 7.18E-5
18 SGTC_2009 4012297 3.80 12.1 µM 6.60E-5 cell wall signaling 66 7.34E-5
19 SGTC_749 1000-0129 3.77 128.0 µM 7.49E-5 amide catabolism 23 8.31E-5
20 SGTC_65 0798-0905 3.65 35.0 µM 1.17E-4 calcium & mitochondrial duress 105 1.29E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.154 2.65E-19 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.139 5.30E-16 YBL082C ALG3 hom Dolichol-P-Man dependent alpha(1-3) mannosyltransferase, involved in the synthesis of dolichol-linked oligosaccharide donor for N-linked glycosylation of proteins
0.133 1.05E-14 YDR246W-A_p YDR246W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.129 6.96E-14 YDR156W RPA14 hom RNA polymerase I subunit A14
0.127 1.95E-13 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.123 7.63E-13 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.122 1.07E-12 YDR500C RPL37B hom Ribosomal 60S subunit protein L37B; protein abundance increases in response to DNA replication stress; homologous to mammalian ribosomal protein L37, no bacterial homolog; RPL37B has a paralog, RPL37A, that arose from the whole genome duplication
0.121 2.24E-12 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication
0.119 4.99E-12 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.118 6.26E-12 YOR216C RUD3 hom Golgi matrix protein involved in the structural organization of the cis-Golgi; interacts genetically with COG3 and USO1
0.117 1.10E-11 YNL219C ALG9 hom Mannosyltransferase, involved in N-linked glycosylation; catalyzes both the transfer of seventh mannose residue on B-arm and ninth mannose residue on the C-arm from Dol-P-Man to lipid-linked oligosaccharides; mutation of the human ortholog causes type 1 congenital disorders of glycosylation
0.116 1.77E-11 YML019W OST6 hom Subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; similar to and partially functionally redundant with Ost3p
0.114 3.52E-11 YGL079W_p KXD1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; GFP-fusion protein localizes to the endosome
0.108 4.05E-10 YBL083C_d YBL083C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps verified ORF ALG3
0.105 9.20E-10 YPL033C SRL4 hom Protein of unknown function; involved in regulation of dNTP production; null mutant suppresses the lethality of lcd1 and rad53 mutations; expression is induced by Kar4p