YGR240C / PFK1

Alpha subunit of heterooctameric phosphofructokinase involved in glycolysis, indispensable for anaerobic growth, activated by fructose-2,6-bisphosphate and AMP, mutation inhibits glucose induction of cell cycle-related genes

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR240C / PFK1

Click on Significant Values for Screen Details ID:SGTC_70|Compound:0141-0289|FD-Score:6.14|P-value:5.18E-8 ID:SGTC_109|Compound:0132-0036|FD-Score:4.85|P-value:1.31E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:3.96|P-value:3.06E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:4.04|P-value:2.34E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.60|P-value:9.27E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-4.34|P-value:8.32E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:3.95|P-value:3.10E-4 ID:SGTC_487|Compound:nimodipine|FD-Score:3.74|P-value:5.97E-4 ID:SGTC_489|Compound:paxilline|FD-Score:5.00|P-value:7.47E-6 ID:SGTC_491|Compound:tetrandrine|FD-Score:4.50|P-value:4.78E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:4.24|P-value:1.20E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.90|P-value:3.67E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:-3.59|P-value:9.19E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:4.31|P-value:9.54E-5 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.59|P-value:9.17E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:4.59|P-value:3.49E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:4.35|P-value:8.14E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:3.83|P-value:4.61E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.69|P-value:7.06E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:4.31|P-value:9.59E-5 ID:SGTC_948|Compound:3448-0295|FD-Score:5.88|P-value:1.74E-7 ID:SGTC_972|Compound:1319-0219|FD-Score:3.99|P-value:2.73E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.25|P-value:1.17E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:9.89|P-value:5.28E-18 ID:SGTC_1197|Compound:1601-0112|FD-Score:5.68|P-value:4.24E-7 ID:SGTC_1284|Compound:0929-0043|FD-Score:4.04|P-value:2.34E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:5.18|P-value:3.67E-6 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.17|P-value:1.52E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:4.67|P-value:2.64E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:6.98|P-value:7.35E-10 ID:SGTC_1505|Compound:k297-0037|FD-Score:4.11|P-value:1.86E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.60|P-value:9.05E-4 ID:SGTC_1825|Compound:st052143|FD-Score:5.00|P-value:7.41E-6 ID:SGTC_1900|Compound:st060200|FD-Score:7.95|P-value:2.83E-12 ID:SGTC_1936|Compound:st074407|FD-Score:-3.92|P-value:3.34E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-4.41|P-value:6.48E-5 ID:SGTC_2188|Compound:6126565|FD-Score:4.28|P-value:1.04E-4 ID:SGTC_2193|Compound:5925765|FD-Score:3.78|P-value:5.25E-4 ID:SGTC_2196|Compound:6573537|FD-Score:-4.22|P-value:1.26E-4 ID:SGTC_2214|Compound:7179297|FD-Score:5.30|P-value:2.19E-6 ID:SGTC_2226|Compound:7298446|FD-Score:5.02|P-value:6.90E-6 ID:SGTC_2321|Compound:7390090|FD-Score:5.99|P-value:1.04E-7 ID:SGTC_2339|Compound:9000433|FD-Score:4.43|P-value:6.17E-5 ID:SGTC_2396|Compound:5342950|FD-Score:4.20|P-value:1.36E-4 ID:SGTC_2478|Compound:5763493|FD-Score:4.05|P-value:2.27E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.82|P-value:4.77E-4 ID:SGTC_2902|Compound:5787006|FD-Score:-4.11|P-value:1.77E-4 ID:SGTC_2938|Compound:9059746|FD-Score:5.51|P-value:9.05E-7 ID:SGTC_3016|Compound:9080866|FD-Score:5.20|P-value:3.36E-6 ID:SGTC_3074|Compound:9116018|FD-Score:6.14|P-value:5.22E-8 ID:SGTC_70|Compound:0141-0289|FD-Score:6.14|P-value:5.18E-8 ID:SGTC_109|Compound:0132-0036|FD-Score:4.85|P-value:1.31E-5 ID:SGTC_119|Compound:0121-0043|FD-Score:3.96|P-value:3.06E-4 ID:SGTC_331|Compound:k048-0133|FD-Score:4.04|P-value:2.34E-4 ID:SGTC_422|Compound:cantharidin|FD-Score:3.60|P-value:9.27E-4 ID:SGTC_424|Compound:splitomicin|FD-Score:-4.34|P-value:8.32E-5 ID:SGTC_435|Compound:c-PAF|FD-Score:3.95|P-value:3.10E-4 ID:SGTC_487|Compound:nimodipine|FD-Score:3.74|P-value:5.97E-4 ID:SGTC_489|Compound:paxilline|FD-Score:5.00|P-value:7.47E-6 ID:SGTC_491|Compound:tetrandrine|FD-Score:4.50|P-value:4.78E-5 ID:SGTC_530|Compound:4358-1714|FD-Score:4.24|P-value:1.20E-4 ID:SGTC_542|Compound:3381-0244|FD-Score:3.90|P-value:3.67E-4 ID:SGTC_607|Compound:4491-0986|FD-Score:-3.59|P-value:9.19E-4 ID:SGTC_666|Compound:0272-0154|FD-Score:4.31|P-value:9.54E-5 ID:SGTC_674|Compound:0498-0004|FD-Score:-3.59|P-value:9.17E-4 ID:SGTC_756|Compound:0508-1999|FD-Score:4.59|P-value:3.49E-5 ID:SGTC_801|Compound:0866-0028|FD-Score:4.35|P-value:8.14E-5 ID:SGTC_860|Compound:0443-0016|FD-Score:3.83|P-value:4.61E-4 ID:SGTC_877|Compound:0771-0151|FD-Score:3.69|P-value:7.06E-4 ID:SGTC_913|Compound:3487-1245|FD-Score:4.31|P-value:9.59E-5 ID:SGTC_948|Compound:3448-0295|FD-Score:5.88|P-value:1.74E-7 ID:SGTC_972|Compound:1319-0219|FD-Score:3.99|P-value:2.73E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.25|P-value:1.17E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:9.89|P-value:5.28E-18 ID:SGTC_1197|Compound:1601-0112|FD-Score:5.68|P-value:4.24E-7 ID:SGTC_1284|Compound:0929-0043|FD-Score:4.04|P-value:2.34E-4 ID:SGTC_1368|Compound:1852-0141|FD-Score:5.18|P-value:3.67E-6 ID:SGTC_1386|Compound:3228-0095|FD-Score:4.17|P-value:1.52E-4 ID:SGTC_1445|Compound:4239-0156|FD-Score:4.67|P-value:2.64E-5 ID:SGTC_1458|Compound:k015-0034|FD-Score:6.98|P-value:7.35E-10 ID:SGTC_1505|Compound:k297-0037|FD-Score:4.11|P-value:1.86E-4 ID:SGTC_1593|Compound:justicidin b . 9-(1,3-benzodioxol-5-yl)- 6,7-dimethoxynaphtho[2,3-c]furan-1(3h)-one|FD-Score:3.60|P-value:9.05E-4 ID:SGTC_1825|Compound:st052143|FD-Score:5.00|P-value:7.41E-6 ID:SGTC_1900|Compound:st060200|FD-Score:7.95|P-value:2.83E-12 ID:SGTC_1936|Compound:st074407|FD-Score:-3.92|P-value:3.34E-4 ID:SGTC_2033|Compound:5192550|FD-Score:-4.41|P-value:6.48E-5 ID:SGTC_2188|Compound:6126565|FD-Score:4.28|P-value:1.04E-4 ID:SGTC_2193|Compound:5925765|FD-Score:3.78|P-value:5.25E-4 ID:SGTC_2196|Compound:6573537|FD-Score:-4.22|P-value:1.26E-4 ID:SGTC_2214|Compound:7179297|FD-Score:5.30|P-value:2.19E-6 ID:SGTC_2226|Compound:7298446|FD-Score:5.02|P-value:6.90E-6 ID:SGTC_2321|Compound:7390090|FD-Score:5.99|P-value:1.04E-7 ID:SGTC_2339|Compound:9000433|FD-Score:4.43|P-value:6.17E-5 ID:SGTC_2396|Compound:5342950|FD-Score:4.20|P-value:1.36E-4 ID:SGTC_2478|Compound:5763493|FD-Score:4.05|P-value:2.27E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:3.82|P-value:4.77E-4 ID:SGTC_2902|Compound:5787006|FD-Score:-4.11|P-value:1.77E-4 ID:SGTC_2938|Compound:9059746|FD-Score:5.51|P-value:9.05E-7 ID:SGTC_3016|Compound:9080866|FD-Score:5.20|P-value:3.36E-6 ID:SGTC_3074|Compound:9116018|FD-Score:6.14|P-value:5.22E-8

Top fitness defect scores for YGR240C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1166 k206-0078 9.89 225.0 µM 5.28E-18 1 2.39E-23
2 SGTC_1900 st060200 7.95 72.8 µM 2.83E-12 2 9.37E-16
3 SGTC_1458 k015-0034 6.98 6.3 µM 7.35E-10 1 1.47E-12
4 SGTC_70 0141-0289 6.14 43.3 µM 5.18E-8 mitochondrial stress 78 4.10E-10
5 SGTC_3074 9116018 6.14 49.5 µM 5.22E-8 RNA processing & uracil transport 4 4.14E-10
6 SGTC_2321 7390090 5.99 138.8 µM 1.04E-7 6 1.03E-9
7 SGTC_948 3448-0295 5.88 75.3 µM 1.74E-7 5 2.03E-9
8 SGTC_1197 1601-0112 5.68 267.0 µM 4.24E-7 2 6.58E-9
9 SGTC_2938 9059746 5.51 27.3 µM 9.05E-7 fatty acid desaturase (OLE1) 16 1.79E-8
10 SGTC_2214 7179297 5.30 48.3 µM 2.19E-6 superoxide 4 5.74E-8
11 SGTC_3016 9080866 5.20 49.5 µM 3.36E-6 7 1.01E-7
12 SGTC_1368 1852-0141 5.18 284.0 µM 3.67E-6 ERG2 20 1.13E-7
13 SGTC_2226 7298446 5.02 200.0 µM 6.90E-6 2 2.60E-7
14 SGTC_1825 st052143 5.00 62.6 µM 7.41E-6 6 2.86E-7
15 SGTC_489 paxilline 5.00 115.0 µM 7.47E-6 PDR1 5 2.89E-7
16 SGTC_109 0132-0036 4.85 33.7 µM 1.31E-5 17 6.05E-7
17 SGTC_1445 4239-0156 4.67 97.7 µM 2.64E-5 12 1.52E-6
18 SGTC_756 0508-1999 4.59 28.3 µM 3.49E-5 RPP1 & pyrimidine depletion 12 2.19E-6
19 SGTC_491 tetrandrine 4.50 80.3 µM 4.78E-5 7 3.32E-6
20 SGTC_2339 9000433 4.43 200.0 µM 6.17E-5 22 4.65E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.109 2.65E-10 YEL042W GDA1 hom Guanosine diphosphatase located in the Golgi, involved in the transport of GDP-mannose into the Golgi lumen by converting GDP to GMP after mannose is transferred its substrate
0.091 1.32E-7 YJL143W TIM17 het Essential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); with Tim23p, contributes to the architecture and function of the import channel; may link the import motor to the core TIM23 complex
0.087 4.91E-7 YHR155W YSP1 hom Mitochondrial protein; potential role in promoting mitochondrial fragmentation during programmed cell death in response to high levels of alpha-factor mating pheromone or the drug amiodarone; YSP1 has a paralog, SIP3, that arose from the whole genome duplication
0.084 1.21E-6 YFL041W-A_p YFL041W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.077 8.39E-6 YDR414C ERD1 hom Predicted membrane protein required for the retention of lumenal endoplasmic reticulum proteins; mutants secrete the endogenous ER protein, BiP (Kar2p)
0.076 1.03E-5 YLR070C XYL2 hom Xylitol dehydrogenase, converts xylitol to D-xylulose; expression induced by xylose, even though this pentose sugar is not well utilized by S. cerevisiae; null mutant has cell wall defect
0.074 1.90E-5 YOR109W INP53 hom Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.073 2.22E-5 YNL039W BDP1 het Essential subunit of RNA polymerase III transcription factor (TFIIIB), which is involved in transcription of genes encoding tRNAs, 5S rRNA, U6 snRNA, and other small RNAs
0.070 4.56E-5 YDR065W RRG1 hom Protein of unknown function, required for vacuolar acidification and mitochondrial genome maintenance; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.070 4.73E-5 YLR342W FKS1 hom Catalytic subunit of 1,3-beta-D-glucan synthase, functionally redundant with alternate catalytic subunit Gsc2p; binds to regulatory subunit Rho1p; involved in cell wall synthesis and maintenance; localizes to sites of cell wall remodeling
0.066 1.25E-4 YOR055W_d YOR055W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.065 1.51E-4 YLL040C VPS13 hom Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.065 1.70E-4 YDR529C QCR7 hom Subunit 7 of the ubiquinol cytochrome-c reductase complex, which is a component of the mitochondrial inner membrane electron transport chain; oriented facing the mitochondrial matrix; N-terminus appears to play a role in complex assembly
0.065 1.84E-4 YNL257C SIP3 hom Transcription cofactor; acts through interaction with DNA-bound Snf1p; C-terminal region has a putative leucine zipper motif; potential Cdc28p substrate; SIP3 has a paralog, YSP1, that arose from the whole genome duplication
0.064 2.04E-4 YOR021C SFM1 hom SPOUT methyltransferase; catalyzes omega-monomethylation of Rps3p on Arg-146; not an essential gene; predicted to be involved in rRNA processing and ribosome biogenesis and in biopolymer catabolism