YGR245C / SDA1

Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR245C / SDA1

Click on Significant Values for Screen Details ID:SGTC_40|Compound:0327-0103|FD-Score:3.73|P-value:2.65E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-4.17|P-value:3.97E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-10.60|P-value:7.76E-24 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.40|P-value:7.85E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.34|P-value:9.46E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:6.33|P-value:1.72E-9 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.58|P-value:3.51E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.46|P-value:6.54E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.84|P-value:1.75E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.37|P-value:8.66E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.35|P-value:9.23E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.73|P-value:2.04E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.45|P-value:6.80E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.44|P-value:7.02E-4 ID:SGTC_2081|Compound:5214970|FD-Score:5.06|P-value:1.21E-6 ID:SGTC_2425|Compound:5628681|FD-Score:3.41|P-value:7.65E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.70|P-value:2.90E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-4.61|P-value:6.40E-6 ID:SGTC_2794|Compound:7785382|FD-Score:-3.49|P-value:4.72E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-7.31|P-value:2.73E-12 ID:SGTC_2803|Compound:7707263|FD-Score:-4.21|P-value:3.37E-5 ID:SGTC_2979|Compound:9012552|FD-Score:4.04|P-value:8.38E-5 ID:SGTC_2984|Compound:9022109|FD-Score:3.64|P-value:3.56E-4 ID:SGTC_40|Compound:0327-0103|FD-Score:3.73|P-value:2.65E-4 ID:SGTC_366|Compound:0181-0206|FD-Score:-4.17|P-value:3.97E-5 ID:SGTC_372|Compound:0155-0182|FD-Score:-10.60|P-value:7.76E-24 ID:SGTC_409|Compound:arachidonic acid|FD-Score:3.40|P-value:7.85E-4 ID:SGTC_562|Compound:3772-2733|FD-Score:3.34|P-value:9.46E-4 ID:SGTC_664|Compound:1222-0054|FD-Score:6.33|P-value:1.72E-9 ID:SGTC_665|Compound:3914-0008|FD-Score:-3.58|P-value:3.51E-4 ID:SGTC_1298|Compound:1079-1278|FD-Score:3.46|P-value:6.54E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.84|P-value:1.75E-4 ID:SGTC_1321|Compound:1326-2144|FD-Score:3.37|P-value:8.66E-4 ID:SGTC_1427|Compound:4048-4900|FD-Score:3.35|P-value:9.23E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-3.73|P-value:2.04E-4 ID:SGTC_1564|Compound:D-Arginine|FD-Score:3.45|P-value:6.80E-4 ID:SGTC_1975|Compound:st070967|FD-Score:3.44|P-value:7.02E-4 ID:SGTC_2081|Compound:5214970|FD-Score:5.06|P-value:1.21E-6 ID:SGTC_2425|Compound:5628681|FD-Score:3.41|P-value:7.65E-4 ID:SGTC_2528|Compound:xanthyletin|FD-Score:3.70|P-value:2.90E-4 ID:SGTC_2693|Compound:st077581|FD-Score:-4.61|P-value:6.40E-6 ID:SGTC_2794|Compound:7785382|FD-Score:-3.49|P-value:4.72E-4 ID:SGTC_2797|Compound:7859098|FD-Score:-7.31|P-value:2.73E-12 ID:SGTC_2803|Compound:7707263|FD-Score:-4.21|P-value:3.37E-5 ID:SGTC_2979|Compound:9012552|FD-Score:4.04|P-value:8.38E-5 ID:SGTC_2984|Compound:9022109|FD-Score:3.64|P-value:3.56E-4

Top fitness defect scores for YGR245C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_664 1222-0054 6.33 14.9 µM 1.72E-9 sphingolipid biosynthesis & PDR1 3 1.23E-10 0.89
2 SGTC_2081 5214970 5.06 32.3 µM 1.21E-6 1 2.14E-7 1.04
3 SGTC_2979 9012552 4.04 45.5 µM 8.38E-5 1 2.63E-5 0.69
4 SGTC_1305 1181-0477 3.84 144.0 µM 1.75E-4 4 6.08E-5 0.26
5 SGTC_40 0327-0103 3.73 19.8 µM 2.65E-4 6 9.71E-5 0.12
6 SGTC_2528 xanthyletin 3.70 44.8 µM 2.90E-4 7 1.08E-4 0.34
7 SGTC_2984 9022109 3.64 39.0 µM 3.56E-4 4 1.36E-4 0.07
8 SGTC_1298 1079-1278 3.46 263.0 µM 6.54E-4 DNA damage response 8 2.71E-4 0.14
9 SGTC_1564 D-Arginine 3.45 115.0 µM 6.80E-4 11 2.84E-4 0.07
10 SGTC_1975 st070967 3.44 30.8 µM 7.02E-4 16 2.94E-4 0.01
11 SGTC_2425 5628681 3.41 7.8 µM 7.65E-4 9 3.24E-4 0.34
12 SGTC_409 arachidonic acid 3.40 100.0 µM 7.85E-4 5 3.34E-4 0.18
13 SGTC_1321 1326-2144 3.37 215.0 µM 8.66E-4 7 3.73E-4 0.02
14 SGTC_1427 4048-4900 3.35 550.0 nM 9.23E-4 4 4.01E-4 0.04
15 SGTC_562 3772-2733 3.34 112.0 µM 9.46E-4 ERG2 18 4.12E-4 0.01
16 SGTC_2535 4',4'-dimethoxydalbergione 3.29 51.2 µM 0.00111 DNA damage response 7 4.96E-4 0.23
17 SGTC_2007 4011539 3.28 71.8 µM 0.00117 cell wall signaling 13 5.26E-4 0.21
18 SGTC_3000 9075062 3.25 71.4 µM 0.00126 11 5.70E-4 0.04
19 SGTC_747 3448-8087 3.24 38.7 µM 0.00130 12 5.94E-4 0.32
20 SGTC_1024 4507-0395 3.22 26.0 µM 0.00137 TSC3-RPN4 17 6.30E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.205 2.96E-33 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.168 9.26E-23 YPL231W FAS2 het Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
0.154 3.09E-19 YLR167W RPS31 het Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
0.152 7.37E-19 YHR164C DNA2 het Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress
0.143 9.22E-17 YLR101C_d YLR101C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified, essential ORF ERG27/YLR100W
0.142 1.20E-16 YOL026C MIM1 het Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex
0.142 1.27E-16 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.136 2.95E-15 YEL019C MMS21 het SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
0.135 4.05E-15 YML065W ORC1 het Largest subunit of the origin recognition complex, which directs DNA replication by binding to replication origins and is also involved in transcriptional silencing; exhibits ATPase activity
0.133 9.46E-15 YOL097C WRS1 het Cytoplasmic tryptophanyl-tRNA synthetase, aminoacylates tryptophanyl-tRNA
0.127 1.41E-13 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.125 3.50E-13 YKL033W TTI1 het Subunit of the ASTRA complex, involved in chromatin remodeling; telomere length regulator involved in the stability or biogenesis of PIKKs such as TORC1; similar to S. pombe Tti1p; detected in highly purified mitochondria in high-throughput studies
0.125 4.44E-13 YGL044C RNA15 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.122 1.41E-12 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.122 1.55E-12 YOR370C MRS6 het Rab escort protein; forms a complex with the Ras-like small GTPase Ypt1p that is required for the prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p); sequence similarity to mammalian choroideraemia gene; relative distribution to the nucleus increases upon DNA replication stress