YGR246C / BRF1

TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR246C / BRF1

Click on Significant Values for Screen Details ID:SGTC_64|Compound:000l-0018|FD-Score:-3.20|P-value:2.54E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-5.05|P-value:1.66E-8 ID:SGTC_162|Compound:k030-0015|FD-Score:2.97|P-value:4.57E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.34|P-value:1.06E-6 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.28|P-value:1.77E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.25|P-value:1.47E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:2.96|P-value:4.68E-4 ID:SGTC_324|Compound:1315-0088|FD-Score:2.83|P-value:7.88E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:2.96|P-value:4.77E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.71|P-value:2.59E-5 ID:SGTC_740|Compound:k014-0057|FD-Score:3.54|P-value:4.08E-5 ID:SGTC_804|Compound:1595-0054|FD-Score:3.49|P-value:5.08E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:2.84|P-value:7.70E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.16|P-value:2.95E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.84|P-value:1.41E-5 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.04|P-value:3.40E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:-2.92|P-value:7.60E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.35|P-value:1.31E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.48|P-value:5.40E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.34|P-value:1.06E-6 ID:SGTC_2060|Compound:5238662|FD-Score:-4.59|P-value:2.68E-7 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:3.20|P-value:1.76E-4 ID:SGTC_2174|Compound:5790901|FD-Score:-3.01|P-value:5.35E-4 ID:SGTC_2176|Compound:5809774|FD-Score:-2.87|P-value:9.04E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.56|P-value:5.18E-5 ID:SGTC_2259|Compound:7964009|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.95|P-value:5.43E-6 ID:SGTC_2343|Compound:7990716|FD-Score:4.42|P-value:4.55E-7 ID:SGTC_2346|Compound:9031566|FD-Score:-3.69|P-value:2.84E-5 ID:SGTC_2348|Compound:9022543|FD-Score:-2.91|P-value:7.73E-4 ID:SGTC_2357|Compound:9067248|FD-Score:4.44|P-value:3.99E-7 ID:SGTC_2395|Compound:5646831|FD-Score:3.07|P-value:3.03E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.16|P-value:2.92E-4 ID:SGTC_2437|Compound:5741531|FD-Score:2.80|P-value:8.86E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:4.15|P-value:1.98E-6 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:2.85|P-value:7.26E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.48|P-value:5.27E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:3.67|P-value:2.23E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:2.82|P-value:8.25E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:3.89|P-value:7.52E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.10|P-value:3.82E-6 ID:SGTC_2693|Compound:st077581|FD-Score:-4.24|P-value:1.85E-6 ID:SGTC_2832|Compound:7992762|FD-Score:3.16|P-value:2.14E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.10|P-value:3.80E-4 ID:SGTC_3192|Compound:9110362|FD-Score:3.06|P-value:3.23E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-3.84|P-value:1.43E-5 ID:SGTC_3263|Compound:9137018|FD-Score:-2.91|P-value:7.79E-4 ID:SGTC_3274|Compound:9138298|FD-Score:3.22|P-value:1.66E-4 ID:SGTC_3281|Compound:9140613|FD-Score:-3.34|P-value:1.37E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-2.93|P-value:7.22E-4 ID:SGTC_3304|Compound:9125678|FD-Score:2.97|P-value:4.51E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:-3.20|P-value:2.54E-4 ID:SGTC_73|Compound:0327-0325|FD-Score:-5.05|P-value:1.66E-8 ID:SGTC_162|Compound:k030-0015|FD-Score:2.97|P-value:4.57E-4 ID:SGTC_166|Compound:k072-0058|FD-Score:-4.34|P-value:1.06E-6 ID:SGTC_167|Compound:3672-0051|FD-Score:-3.28|P-value:1.77E-4 ID:SGTC_169|Compound:k015-0017|FD-Score:3.25|P-value:1.47E-4 ID:SGTC_181|Compound:1447-1644|FD-Score:2.96|P-value:4.68E-4 ID:SGTC_324|Compound:1315-0088|FD-Score:2.83|P-value:7.88E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:2.96|P-value:4.77E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.71|P-value:2.59E-5 ID:SGTC_740|Compound:k014-0057|FD-Score:3.54|P-value:4.08E-5 ID:SGTC_804|Compound:1595-0054|FD-Score:3.49|P-value:5.08E-5 ID:SGTC_809|Compound:3998-0021|FD-Score:2.84|P-value:7.70E-4 ID:SGTC_1018|Compound:4259-0013|FD-Score:-3.16|P-value:2.95E-4 ID:SGTC_1249|Compound:0481-0015|FD-Score:-3.84|P-value:1.41E-5 ID:SGTC_1323|Compound:1348-1351|FD-Score:3.04|P-value:3.40E-4 ID:SGTC_1338|Compound:1486-1223|FD-Score:-2.92|P-value:7.60E-4 ID:SGTC_1789|Compound:tunicamycin|FD-Score:-3.35|P-value:1.31E-4 ID:SGTC_1890|Compound:3',4',7',8'-tetrahydroxyflavone|FD-Score:3.48|P-value:5.40E-5 ID:SGTC_2054|Compound:5233173|FD-Score:-4.34|P-value:1.06E-6 ID:SGTC_2060|Compound:5238662|FD-Score:-4.59|P-value:2.68E-7 ID:SGTC_2091|Compound:4-phenylthiadiazole|FD-Score:3.20|P-value:1.76E-4 ID:SGTC_2174|Compound:5790901|FD-Score:-3.01|P-value:5.35E-4 ID:SGTC_2176|Compound:5809774|FD-Score:-2.87|P-value:9.04E-4 ID:SGTC_2199|Compound:6590217|FD-Score:-3.56|P-value:5.18E-5 ID:SGTC_2259|Compound:7964009|FD-Score:3.19|P-value:1.87E-4 ID:SGTC_2342|Compound:7990637|FD-Score:3.95|P-value:5.43E-6 ID:SGTC_2343|Compound:7990716|FD-Score:4.42|P-value:4.55E-7 ID:SGTC_2346|Compound:9031566|FD-Score:-3.69|P-value:2.84E-5 ID:SGTC_2348|Compound:9022543|FD-Score:-2.91|P-value:7.73E-4 ID:SGTC_2357|Compound:9067248|FD-Score:4.44|P-value:3.99E-7 ID:SGTC_2395|Compound:5646831|FD-Score:3.07|P-value:3.03E-4 ID:SGTC_2424|Compound:5537928|FD-Score:-3.16|P-value:2.92E-4 ID:SGTC_2437|Compound:5741531|FD-Score:2.80|P-value:8.86E-4 ID:SGTC_2596|Compound:sinensetin|FD-Score:4.15|P-value:1.98E-6 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:2.85|P-value:7.26E-4 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.48|P-value:5.27E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:3.67|P-value:2.23E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:2.82|P-value:8.25E-4 ID:SGTC_2684|Compound:capsaicin|FD-Score:3.89|P-value:7.52E-6 ID:SGTC_2687|Compound:chenodiol|FD-Score:-4.10|P-value:3.82E-6 ID:SGTC_2693|Compound:st077581|FD-Score:-4.24|P-value:1.85E-6 ID:SGTC_2832|Compound:7992762|FD-Score:3.16|P-value:2.14E-4 ID:SGTC_2935|Compound:9038016|FD-Score:-3.10|P-value:3.80E-4 ID:SGTC_3192|Compound:9110362|FD-Score:3.06|P-value:3.23E-4 ID:SGTC_3208|Compound:9114115|FD-Score:-3.84|P-value:1.43E-5 ID:SGTC_3263|Compound:9137018|FD-Score:-2.91|P-value:7.79E-4 ID:SGTC_3274|Compound:9138298|FD-Score:3.22|P-value:1.66E-4 ID:SGTC_3281|Compound:9140613|FD-Score:-3.34|P-value:1.37E-4 ID:SGTC_3283|Compound:9109332|FD-Score:-2.93|P-value:7.22E-4 ID:SGTC_3304|Compound:9125678|FD-Score:2.97|P-value:4.51E-4

Top fitness defect scores for YGR246C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2357 9067248 4.44 200.0 µM 3.99E-7 2 4.47E-6 0.59
2 SGTC_2343 7990716 4.42 14.8 µM 4.55E-7 2 4.97E-6 0.46
3 SGTC_2596 sinensetin 4.15 100.0 µM 1.98E-6 3 1.67E-5 0.15
4 SGTC_2342 7990637 3.95 118.9 µM 5.43E-6 RNA processing & uracil transport 7 3.84E-5 0.23
5 SGTC_2684 capsaicin 3.89 100.0 µM 7.52E-6 6 5.02E-5 0.23
6 SGTC_2620 irigenin 3.67 100.0 µM 2.23E-5 19 1.23E-4 0.10
7 SGTC_740 k014-0057 3.54 261.0 µM 4.08E-5 2 2.02E-4 0.02
8 SGTC_804 1595-0054 3.49 20.9 µM 5.08E-5 sphingolipid biosynthesis & PDR1 7 2.42E-4 0.25
9 SGTC_2618 avocadyne 3.48 43.4 µM 5.27E-5 5 2.50E-4 0.14
10 SGTC_1890 3',4',7',8'-tetrahydroxyflavone 3.48 14.9 µM 5.40E-5 11 2.55E-4 0.05
11 SGTC_169 k015-0017 3.25 16.0 µM 1.47E-4 8 5.83E-4 0.19
12 SGTC_3274 9138298 3.22 49.5 µM 1.66E-4 11 6.44E-4 0.12
13 SGTC_2091 4-phenylthiadiazole 3.20 200.0 µM 1.76E-4 4 6.77E-4 0.01
14 SGTC_2259 7964009 3.19 200.0 µM 1.87E-4 superoxide 10 7.12E-4 0.07
15 SGTC_2832 7992762 3.16 52.0 µM 2.14E-4 13 7.92E-4 0.01
16 SGTC_2395 5646831 3.07 180.2 µM 3.03E-4 9 0.00106 0.01
17 SGTC_3192 9110362 3.06 49.5 µM 3.23E-4 mitochondrial processes 16 0.00112 0.11
18 SGTC_1323 1348-1351 3.04 11.2 µM 3.40E-4 6 0.00116 0.08
19 SGTC_3304 9125678 2.97 46.1 µM 4.51E-4 38 0.00147 0.10
20 SGTC_162 k030-0015 2.97 37.8 µM 4.57E-4 iron homeostasis 11 0.00149 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.143 1.06E-16 YKL164C PIR1 hom O-glycosylated protein required for cell wall stability; attached to the cell wall via beta-1,3-glucan; mediates mitochondrial translocation of Apn1p; expression regulated by the cell integrity pathway and by Swi5p during the cell cycle; PIR1 has a paralog, YJL160C, that arose from the whole genome duplication
0.136 2.53E-15 YDL008W APC11 het Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
0.111 1.42E-10 YGL249W ZIP2 hom Meiosis-specific protein; involved in normal synaptonemal complex formation and pairing between homologous chromosomes during meiosis; relocalizes from mitochondrion to cytoplasm upon DNA replication stress
0.103 1.86E-9 YMR017W SPO20 hom Meiosis-specific subunit of the t-SNARE complex, required for prospore membrane formation during sporulation; similar to but not functionally redundant with Sec9p; SNAP-25 homolog
0.103 1.93E-9 YCR102W-A_d YCR102W-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.098 1.33E-8 YFL063W_d YFL063W_d hom Dubious open reading frame, based on available experimental and comparative sequence data
0.097 1.64E-8 YGR276C RNH70 hom 3'-5' exoribonuclease; required for maturation of 3' ends of 5S rRNA and tRNA-Arg3 from dicistronic transcripts
0.097 1.78E-8 YGR068C ART5 hom Protein proposed to regulate the endocytosis of plasma membrane proteins by recruiting the ubiquitin ligase Rsp5p to its target in the plasma membrane
0.097 2.16E-8 YDR048C_d YDR048C_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.095 3.21E-8 YPR012W_d YPR012W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPR012W is not an essential gene
0.095 3.36E-8 YDR469W SDC1 hom Subunit of the COMPASS (Set1C) complex, which methylates lysine 4 of histone H3 and is required in chromatin silencing at telomeres; contains a Dpy-30 domain that mediates interaction with Bre2p; similar to C. elegans and human DPY-30
0.092 9.06E-8 YDR434W GPI17 het Transmembrane protein subunit of the glycosylphosphatidylinositol transamidase complex that adds GPIs to newly synthesized proteins; human PIG-S homolog
0.091 1.29E-7 YER171W RAD3 het 5' to 3' DNA helicase; involved in nucleotide excision repair and transcription; subunit of RNA polII initiation factor TFIIH and of Nucleotide Excision Repair Factor 3 (NEF3); homolog of human XPD protein; mutant has aneuploidy tolerance; protein abundance increases in response to DNA replication stress
0.091 1.29E-7 YDR390C UBA2 het Subunit of a heterodimeric nuclear SUMO activating enzyme (E1) with Aos1p; activates Smt3p (SUMO) before its conjugation to proteins (sumoylation), which may play a role in protein targeting; essential for viability
0.090 2.08E-7 YEL040W UTR2 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar to and functionally redundant with Crh1; glycosylphosphatidylinositol (GPI)-anchored protein localized to bud neck