YGR252W / GCN5

Acetyltransferase, modifies N-terminal lysines on histones H2B and H3; acetylates Rsc4p, a subunit of the RSC chromatin-remodeling complex, altering replication stress tolerance; catalytic subunit of the ADA and SAGA histone acetyltransferase complexes; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; greater involvement in repression of RNAPII-dependent transcription than in activation

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR252W / GCN5

Click on Significant Values for Screen Details ID:SGTC_102|Compound:gallobenzophenone|FD-Score:5.69|P-value:1.96E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:4.36|P-value:8.01E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:5.70|P-value:1.89E-5 ID:SGTC_201|Compound:4262-0159|FD-Score:4.77|P-value:2.81E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:6.29|P-value:2.68E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.37|P-value:6.38E-12 ID:SGTC_268|Compound:camptothecin|FD-Score:4.41|P-value:7.22E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.46|P-value:6.32E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:8.06|P-value:2.87E-9 ID:SGTC_562|Compound:3772-2733|FD-Score:5.71|P-value:1.82E-5 ID:SGTC_657|Compound:3534-0529|FD-Score:-4.58|P-value:4.67E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:4.57|P-value:4.77E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:4.83|P-value:2.41E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:8.31|P-value:9.54E-10 ID:SGTC_868|Compound:0527-0199|FD-Score:6.02|P-value:6.75E-6 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.64|P-value:2.35E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:4.64|P-value:4.02E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:-5.11|P-value:1.13E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.60|P-value:4.46E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.44|P-value:6.66E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:5.87|P-value:1.09E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:5.27|P-value:7.02E-5 ID:SGTC_1178|Compound:0988-0033|FD-Score:6.25|P-value:3.17E-6 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.58|P-value:4.66E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.93|P-value:1.85E-4 ID:SGTC_1547|Compound:harmine|FD-Score:6.46|P-value:1.49E-6 ID:SGTC_1621|Compound:st003705|FD-Score:4.28|P-value:9.83E-4 ID:SGTC_1627|Compound:st003713|FD-Score:6.74|P-value:5.55E-7 ID:SGTC_1634|Compound:st006576|FD-Score:-5.76|P-value:1.59E-5 ID:SGTC_1638|Compound:st008370|FD-Score:12.20|P-value:6.28E-19 ID:SGTC_1665|Compound:st014639|FD-Score:4.37|P-value:7.90E-4 ID:SGTC_1780|Compound:st038448|FD-Score:-6.25|P-value:3.16E-6 ID:SGTC_1781|Compound:st038725|FD-Score:9.02|P-value:3.55E-11 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.15|P-value:1.01E-4 ID:SGTC_1881|Compound:5652484|FD-Score:4.58|P-value:4.59E-4 ID:SGTC_1983|Compound:st071813|FD-Score:4.53|P-value:5.24E-4 ID:SGTC_2036|Compound:5160441|FD-Score:6.74|P-value:5.48E-7 ID:SGTC_2147|Compound:5545932|FD-Score:5.68|P-value:2.01E-5 ID:SGTC_2220|Compound:6782180|FD-Score:5.49|P-value:3.56E-5 ID:SGTC_2290|Compound:7904846|FD-Score:5.18|P-value:9.04E-5 ID:SGTC_2292|Compound:7671323|FD-Score:6.05|P-value:6.09E-6 ID:SGTC_2297|Compound:7971671|FD-Score:6.77|P-value:4.85E-7 ID:SGTC_2302|Compound:7771753|FD-Score:-5.70|P-value:1.90E-5 ID:SGTC_2323|Compound:6148992|FD-Score:4.37|P-value:7.96E-4 ID:SGTC_2351|Compound:9026858|FD-Score:5.07|P-value:1.22E-4 ID:SGTC_2397|Compound:5161689|FD-Score:5.08|P-value:1.20E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.39|P-value:7.49E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:5.33|P-value:5.74E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.49|P-value:5.81E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:5.88|P-value:1.07E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:4.74|P-value:3.06E-4 ID:SGTC_2838|Compound:9002687|FD-Score:8.08|P-value:2.55E-9 ID:SGTC_2856|Compound:9022925|FD-Score:-6.67|P-value:7.14E-7 ID:SGTC_2858|Compound:9025781|FD-Score:6.25|P-value:3.07E-6 ID:SGTC_3308|Compound:9128332|FD-Score:5.82|P-value:1.28E-5 ID:SGTC_102|Compound:gallobenzophenone|FD-Score:5.69|P-value:1.96E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:4.36|P-value:8.01E-4 ID:SGTC_178|Compound:4092-0806|FD-Score:5.70|P-value:1.89E-5 ID:SGTC_201|Compound:4262-0159|FD-Score:4.77|P-value:2.81E-4 ID:SGTC_218|Compound:alverine citrate|FD-Score:6.29|P-value:2.68E-6 ID:SGTC_221|Compound:trifluoperazine|FD-Score:9.37|P-value:6.38E-12 ID:SGTC_268|Compound:camptothecin|FD-Score:4.41|P-value:7.22E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.46|P-value:6.32E-4 ID:SGTC_515|Compound:4092-1064|FD-Score:8.06|P-value:2.87E-9 ID:SGTC_562|Compound:3772-2733|FD-Score:5.71|P-value:1.82E-5 ID:SGTC_657|Compound:3534-0529|FD-Score:-4.58|P-value:4.67E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:4.57|P-value:4.77E-4 ID:SGTC_695|Compound:3505-6186|FD-Score:4.83|P-value:2.41E-4 ID:SGTC_706|Compound:4262-0057|FD-Score:8.31|P-value:9.54E-10 ID:SGTC_868|Compound:0527-0199|FD-Score:6.02|P-value:6.75E-6 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.64|P-value:2.35E-5 ID:SGTC_971|Compound:1315-0384|FD-Score:4.64|P-value:4.02E-4 ID:SGTC_985|Compound:1493-0307|FD-Score:-5.11|P-value:1.13E-4 ID:SGTC_1002|Compound:4049-0206|FD-Score:4.60|P-value:4.46E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.44|P-value:6.66E-4 ID:SGTC_1082|Compound:camptothecin|FD-Score:5.87|P-value:1.09E-5 ID:SGTC_1167|Compound:k781-0610|FD-Score:5.27|P-value:7.02E-5 ID:SGTC_1178|Compound:0988-0033|FD-Score:6.25|P-value:3.17E-6 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.58|P-value:4.66E-4 ID:SGTC_1372|Compound:2752-0194|FD-Score:4.93|P-value:1.85E-4 ID:SGTC_1547|Compound:harmine|FD-Score:6.46|P-value:1.49E-6 ID:SGTC_1621|Compound:st003705|FD-Score:4.28|P-value:9.83E-4 ID:SGTC_1627|Compound:st003713|FD-Score:6.74|P-value:5.55E-7 ID:SGTC_1634|Compound:st006576|FD-Score:-5.76|P-value:1.59E-5 ID:SGTC_1638|Compound:st008370|FD-Score:12.20|P-value:6.28E-19 ID:SGTC_1665|Compound:st014639|FD-Score:4.37|P-value:7.90E-4 ID:SGTC_1780|Compound:st038448|FD-Score:-6.25|P-value:3.16E-6 ID:SGTC_1781|Compound:st038725|FD-Score:9.02|P-value:3.55E-11 ID:SGTC_1844|Compound:rapamycin|FD-Score:-5.15|P-value:1.01E-4 ID:SGTC_1881|Compound:5652484|FD-Score:4.58|P-value:4.59E-4 ID:SGTC_1983|Compound:st071813|FD-Score:4.53|P-value:5.24E-4 ID:SGTC_2036|Compound:5160441|FD-Score:6.74|P-value:5.48E-7 ID:SGTC_2147|Compound:5545932|FD-Score:5.68|P-value:2.01E-5 ID:SGTC_2220|Compound:6782180|FD-Score:5.49|P-value:3.56E-5 ID:SGTC_2290|Compound:7904846|FD-Score:5.18|P-value:9.04E-5 ID:SGTC_2292|Compound:7671323|FD-Score:6.05|P-value:6.09E-6 ID:SGTC_2297|Compound:7971671|FD-Score:6.77|P-value:4.85E-7 ID:SGTC_2302|Compound:7771753|FD-Score:-5.70|P-value:1.90E-5 ID:SGTC_2323|Compound:6148992|FD-Score:4.37|P-value:7.96E-4 ID:SGTC_2351|Compound:9026858|FD-Score:5.07|P-value:1.22E-4 ID:SGTC_2397|Compound:5161689|FD-Score:5.08|P-value:1.20E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:4.39|P-value:7.49E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:5.33|P-value:5.74E-5 ID:SGTC_2552|Compound:juarezic acid|FD-Score:4.49|P-value:5.81E-4 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:5.88|P-value:1.07E-5 ID:SGTC_2579|Compound:2',5'-dihydroxy-4-methoxychalcone|FD-Score:4.74|P-value:3.06E-4 ID:SGTC_2838|Compound:9002687|FD-Score:8.08|P-value:2.55E-9 ID:SGTC_2856|Compound:9022925|FD-Score:-6.67|P-value:7.14E-7 ID:SGTC_2858|Compound:9025781|FD-Score:6.25|P-value:3.07E-6 ID:SGTC_3308|Compound:9128332|FD-Score:5.82|P-value:1.28E-5

Top fitness defect scores for YGR252W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1638 st008370 12.20 60.6 µM 6.28E-19 ergosterol biosynthesis 1 1.80E-34
2 SGTC_221 trifluoperazine 9.37 10.3 µM 6.38E-12 NEO1-PIK1 68 3.71E-21
3 SGTC_1781 st038725 9.02 66.3 µM 3.55E-11 NEO1 24 9.62E-20
4 SGTC_706 4262-0057 8.31 46.9 µM 9.54E-10 fatty acid desaturase (OLE1) 5 4.88E-17
5 SGTC_2838 9002687 8.08 71.4 µM 2.55E-9 fatty acid desaturase (OLE1) 4 3.12E-16
6 SGTC_515 4092-1064 8.06 102.0 µM 2.87E-9 plasma membrane duress 18 3.92E-16
7 SGTC_2297 7971671 6.77 152.3 µM 4.85E-7 RNA processing & uracil transport 4 6.23E-12
8 SGTC_2036 5160441 6.74 105.0 µM 5.48E-7 NEO1 36 7.84E-12
9 SGTC_1627 st003713 6.74 25.6 µM 5.55E-7 calcium & mitochondrial duress 21 8.04E-12
10 SGTC_1547 harmine 6.46 94.2 µM 1.49E-6 ergosterol biosynthesis 15 5.16E-11
11 SGTC_218 alverine citrate 6.29 93.8 µM 2.68E-6 fatty acid desaturase (OLE1) 12 1.55E-10
12 SGTC_2858 9025781 6.25 9.0 µM 3.07E-6 sphingolipid biosynthesis & PDR1 42 1.99E-10
13 SGTC_1178 0988-0033 6.25 191.0 µM 3.17E-6 3 2.12E-10
14 SGTC_2292 7671323 6.05 168.1 µM 6.09E-6 RNA processing & uracil transport 8 7.20E-10
15 SGTC_868 0527-0199 6.02 213.0 µM 6.75E-6 fatty acid desaturase (OLE1) 6 8.76E-10
16 SGTC_2562 3',7'-dimethoxyflavone 5.88 64.9 µM 1.07E-5 4 2.07E-9
17 SGTC_1082 camptothecin 5.87 424.9 nM 1.09E-5 DNA damage response 20 2.16E-9
18 SGTC_3308 9128332 5.82 17.6 µM 1.28E-5 plasma membrane duress 39 2.89E-9
19 SGTC_562 3772-2733 5.71 112.0 µM 1.82E-5 ERG2 35 5.61E-9
20 SGTC_178 4092-0806 5.70 87.5 µM 1.89E-5 ergosterol depletion effects on membrane 46 6.00E-9

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.210 8.72E-35 YDL226C GCS1 hom ADP-ribosylation factor GTPase activating protein (ARF GAP), involved in ER-Golgi transport; shares functional similarity with Glo3p
0.202 4.55E-32 YJL207C LAA1 hom AP-1 accessory protein; colocalizes with clathrin to the late-Golgi apparatus; involved in TGN-endosome transport; physically interacts with AP-1; similar to the mammalian p200; may interact with ribosomes; YJL207C is a non-essential gene
0.201 4.82E-32 YDR176W NGG1 hom Transcriptional regulator involved in glucose repression of Gal4p-regulated genes; component of transcriptional adaptor and histone acetyltransferase complexes, the ADA complex, the SAGA complex, and the SLIK complex
0.198 4.13E-31 YER031C YPT31 hom Rab family GTPase; involved in the exocytic pathway; mediates intra-Golgi traffic or the budding of post-Golgi vesicles from the trans-Golgi; YPT31 has a paralog, YPT32, that arose from the whole genome duplication
0.192 3.13E-29 YJL204C RCY1 hom F-box protein involved in recycling plasma membrane proteins internalized by endocytosis; localized to sites of polarized growth
0.188 6.19E-28 YFL034W_p YFL034W_p hom Putative integral membrane protein that interacts with Rpp0p, which is a component of the ribosomal stalk
0.183 1.34E-26 YCR094W CDC50 hom Endosomal protein that interacts with phospholipid flippase Drs2p; interaction with Cdc50p is essential for Drs2p catalytic activity; mutations affect cell polarity and polarized growth; similar to Lem3p; CDC50 has a paralog, YNR048W, that arose from the whole genome duplication
0.177 5.63E-25 YJR060W CBF1 hom Dual function helix-loop-helix protein; binds the motif CACRTG present at several sites including MET gene promoters and centromere DNA element I (CDEI); affects nucleosome positioning at this motif; associates with other transcription factors such as Met4p and Isw1p to mediate transcriptional activation or repression; associates with kinetochore proteins and required for efficient chromosome segregation; protein abundance increases in response to DNA replication stress
0.173 4.76E-24 YMR071C TVP18 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; may interact with ribosomes, based on co-purification experiments
0.168 9.48E-23 YOR070C GYP1 hom Cis-golgi GTPase-activating protein (GAP) for the Rab family members Ypt1p (in vivo) and for Ypt1p, Sec4p, Ypt7p, and Ypt51p (in vitro); involved in vesicle docking and fusion
0.159 2.56E-20 YKL135C APL2 hom Beta-adaptin subunit of the clathrin-associated protein (AP-1) complex; binds clathrin; involved in clathrin-dependent Golgi protein sorting; protein abundance increases in response to DNA replication stress
0.157 6.99E-20 YKL212W SAC1 hom Phosphatidylinositol phosphate (PtdInsP) phosphatase; involved in hydrolysis of PtdIns[4]P in the early and medial Golgi; regulated by interaction with Vps74p; ER localized transmembrane protein which cycles through the Golgi; involved in protein trafficking and processing, secretion, and cell wall maintenance; regulates sphingolipid biosynthesis through the modulation of PtdIns(4)P metabolism
0.155 1.48E-19 YML007C-A_p YML007C-A_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria
0.155 2.02E-19 YLR171W_d YLR171W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.152 8.72E-19 YDL196W_d YDL196W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; open reading frame is located in promoter region of essential gene SEC31