YGR253C / PUP2

Alpha 5 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit zeta

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR253C / PUP2

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.91|P-value:4.78E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:-4.06|P-value:2.65E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:7.27|P-value:3.89E-10 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.99|P-value:3.36E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.56|P-value:5.86E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:4.22|P-value:1.83E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.73|P-value:8.24E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:4.18|P-value:2.11E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:5.90|P-value:3.00E-7 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.69|P-value:3.73E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.94|P-value:4.41E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:3.68|P-value:9.42E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.73|P-value:3.19E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:6.86|P-value:3.32E-9 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.09|P-value:2.42E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:3.90|P-value:5.01E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.86|P-value:4.99E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.63|P-value:9.76E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:6.06|P-value:1.51E-7 ID:SGTC_878|Compound:0828-0289|FD-Score:5.48|P-value:1.78E-6 ID:SGTC_880|Compound:0833-0487|FD-Score:4.30|P-value:1.42E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:3.87|P-value:5.43E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:-3.62|P-value:9.94E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:3.70|P-value:9.08E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.53|P-value:5.68E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.86|P-value:4.88E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:7.83|P-value:1.69E-11 ID:SGTC_1594|Compound:piperine|FD-Score:3.74|P-value:8.05E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:18.90|P-value:2.45E-58 ID:SGTC_1712|Compound:st032288|FD-Score:5.21|P-value:5.26E-6 ID:SGTC_1848|Compound:st056003|FD-Score:3.82|P-value:6.29E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.87|P-value:1.92E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.51|P-value:1.03E-10 ID:SGTC_2320|Compound:7373003|FD-Score:-4.30|P-value:1.24E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.74|P-value:7.09E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.59|P-value:4.57E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.16|P-value:2.25E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-4.32|P-value:1.14E-4 ID:SGTC_2907|Compound:7721985|FD-Score:3.92|P-value:4.72E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.31|P-value:1.18E-4 ID:SGTC_3214|Compound:9129694|FD-Score:-5.09|P-value:7.15E-6 ID:SGTC_3219|Compound:9131112|FD-Score:-6.43|P-value:2.24E-8 ID:SGTC_3241|Compound:9133421|FD-Score:4.17|P-value:2.18E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.91|P-value:4.78E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:-4.06|P-value:2.65E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:7.27|P-value:3.89E-10 ID:SGTC_116|Compound:0155-0182|FD-Score:-3.99|P-value:3.36E-4 ID:SGTC_122|Compound:0236-0016|FD-Score:4.56|P-value:5.86E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:4.22|P-value:1.83E-4 ID:SGTC_138|Compound:0850-0259|FD-Score:3.73|P-value:8.24E-4 ID:SGTC_187|Compound:1488-2104|FD-Score:4.18|P-value:2.11E-4 ID:SGTC_248|Compound:streptozotocin|FD-Score:5.90|P-value:3.00E-7 ID:SGTC_260|Compound:nsc-65238|FD-Score:4.69|P-value:3.73E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.94|P-value:4.41E-4 ID:SGTC_294|Compound:k038-0071|FD-Score:3.68|P-value:9.42E-4 ID:SGTC_312|Compound:4092-0653|FD-Score:4.73|P-value:3.19E-5 ID:SGTC_528|Compound:3492-0019|FD-Score:6.86|P-value:3.32E-9 ID:SGTC_547|Compound:1120-0019|FD-Score:-4.09|P-value:2.42E-4 ID:SGTC_560|Compound:2332-1943|FD-Score:3.90|P-value:5.01E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.86|P-value:4.99E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.63|P-value:9.76E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:6.06|P-value:1.51E-7 ID:SGTC_878|Compound:0828-0289|FD-Score:5.48|P-value:1.78E-6 ID:SGTC_880|Compound:0833-0487|FD-Score:4.30|P-value:1.42E-4 ID:SGTC_930|Compound:2810-3379|FD-Score:3.87|P-value:5.43E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:-3.62|P-value:9.94E-4 ID:SGTC_980|Compound:1473-0395|FD-Score:3.70|P-value:9.08E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:-4.53|P-value:5.68E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-3.86|P-value:4.88E-4 ID:SGTC_1557|Compound:sumaresinolic acid|FD-Score:7.83|P-value:1.69E-11 ID:SGTC_1594|Compound:piperine|FD-Score:3.74|P-value:8.05E-4 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:18.90|P-value:2.45E-58 ID:SGTC_1712|Compound:st032288|FD-Score:5.21|P-value:5.26E-6 ID:SGTC_1848|Compound:st056003|FD-Score:3.82|P-value:6.29E-4 ID:SGTC_1994|Compound:st072947|FD-Score:4.87|P-value:1.92E-5 ID:SGTC_2250|Compound:alverine citrate|FD-Score:7.51|P-value:1.03E-10 ID:SGTC_2320|Compound:7373003|FD-Score:-4.30|P-value:1.24E-4 ID:SGTC_2576|Compound:guaiol|FD-Score:-3.74|P-value:7.09E-4 ID:SGTC_2766|Compound:oltipraz|FD-Score:-4.59|P-value:4.57E-5 ID:SGTC_2778|Compound:tioconazole|FD-Score:4.16|P-value:2.25E-4 ID:SGTC_2831|Compound:7999036|FD-Score:-4.32|P-value:1.14E-4 ID:SGTC_2907|Compound:7721985|FD-Score:3.92|P-value:4.72E-4 ID:SGTC_2965|Compound:9082772|FD-Score:-4.31|P-value:1.18E-4 ID:SGTC_3214|Compound:9129694|FD-Score:-5.09|P-value:7.15E-6 ID:SGTC_3219|Compound:9131112|FD-Score:-6.43|P-value:2.24E-8 ID:SGTC_3241|Compound:9133421|FD-Score:4.17|P-value:2.18E-4

Top fitness defect scores for YGR253C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1597 7-benzyloxygramine 18.90 47.5 µM 2.45E-58 calcium & mitochondrial duress 2 2.29E-80 1.77
2 SGTC_1557 sumaresinolic acid 7.83 42.3 µM 1.69E-11 2 2.37E-15 2.53
3 SGTC_2250 alverine citrate 7.51 130.0 µM 1.03E-10 14 2.90E-14 0.08
4 SGTC_64 000l-0018 7.27 272.1 µM 3.89E-10 ubiquinone biosynthesis & proteasome 23 1.82E-13 0.08
5 SGTC_528 3492-0019 6.86 172.0 µM 3.32E-9 calcium & mitochondrial duress 6 3.53E-12 0.78
6 SGTC_837 0091-0265 6.06 12.5 µM 1.51E-7 ubiquinone biosynthesis & proteasome 2 6.92E-10 0.64
7 SGTC_248 streptozotocin 5.90 5.2 mM 3.00E-7 DNA damage response 10 1.78E-9 0.26
8 SGTC_878 0828-0289 5.48 13.7 µM 1.78E-6 13 2.08E-8 0.48
9 SGTC_1712 st032288 5.21 53.4 µM 5.26E-6 calcium & mitochondrial duress 22 9.24E-8 0.07
10 SGTC_1994 st072947 4.87 40.9 µM 1.92E-5 3 5.49E-7 0.31
11 SGTC_312 4092-0653 4.73 6.8 µM 3.19E-5 ergosterol depletion effects on membrane 24 1.10E-6 0.05
12 SGTC_260 nsc-65238 4.69 4.0 µM 3.73E-5 endomembrane recycling 2 1.37E-6 0.54
13 SGTC_122 0236-0016 4.56 402.4 µM 5.86E-5 RNA processing & uracil transport 12 2.55E-6 0.27
14 SGTC_880 0833-0487 4.30 87.2 µM 1.42E-4 mitochondrial processes 1 8.59E-6 0.73
15 SGTC_137 0438-0332 4.22 85.4 µM 1.83E-4 ubiquinone biosynthesis & proteasome 36 1.22E-5 0.01
16 SGTC_187 1488-2104 4.18 32.7 µM 2.11E-4 calcium & mitochondrial duress 7 1.48E-5 0.17
17 SGTC_3241 9133421 4.17 49.5 µM 2.18E-4 PDR1 1 1.55E-5 1.16
18 SGTC_2778 tioconazole 4.16 65.1 nM 2.25E-4 ergosterol depletion effects on membrane 25 1.61E-5 0.04
19 SGTC_263 alizarin 3.94 107.9 µM 4.41E-4 iron homeostasis 10 4.06E-5 0.09
20 SGTC_2907 7721985 3.92 69.9 µM 4.72E-4 6 4.45E-5 0.07

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.469 2.22E-183 YER094C PUP3 het Beta 3 subunit of the 20S proteasome involved in ubiquitin-dependent catabolism; human homolog is subunit C10
0.437 4.14E-156 YGL011C SCL1 het Alpha 1 subunit of the 20S proteasome involved in the degradation of ubiquitinated substrates; 20S proteasome is the core complex of the 26S proteasome; essential for growth; detected in the mitochondria
0.434 7.54E-154 YMR314W PRE5 het Alpha 6 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.403 4.58E-131 YOR362C PRE10 het Alpha 7 subunit of the 20S proteasome; protein abundance increases in response to DNA replication stress
0.388 1.16E-120 YER012W PRE1 het Beta 4 subunit of the 20S proteasome; localizes to the nucleus throughout the cell cycle
0.383 1.18E-117 YOL038W PRE6 het Alpha 4 subunit of the 20S proteasome; may replace alpha 3 subunit (Pre9p) under stress conditions to create a more active proteasomal isoform; GFP-fusion protein relocates from cytosol to the mitochondrial surface upon oxidative stress
0.364 1.80E-105 YBL041W PRE7 het Beta 6 subunit of the 20S proteasome
0.337 1.43E-89 YML092C PRE8 het Alpha 2 subunit of the 20S proteasome
0.331 1.31E-86 YFR050C PRE4 het Beta 7 subunit of the 20S proteasome
0.331 4.00E-86 YJL165C HAL5 hom Putative protein kinase; overexpression increases sodium and lithium tolerance, whereas gene disruption increases cation and low pH sensitivity and impairs potassium uptake, suggesting a role in regulation of Trk1p and/or Trk2p transporters; HAL5 has a paralog, KKQ8, that arose from the whole genome duplication
0.328 9.38E-85 YJL001W PRE3 het Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides
0.293 3.02E-67 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.273 1.56E-58 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.252 1.44E-49 YLR021W IRC25 hom Component of a heterodimeric Poc4p-Irc25p chaperone involved in assembly of alpha subunits into the 20S proteasome; may regulate formation of proteasome isoforms with alternative subunits under different conditions
0.235 3.81E-43 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane