YGR254W / ENO1

Enolase I, a phosphopyruvate hydratase; catalyzes the conversion of 2-phosphoglycerate to phosphoenolpyruvate during glycolysis and the reverse reaction during gluconeogenesis; expression is repressed in response to glucose; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YGR254W / ENO1

Click on Significant Values for Screen Details ID:SGTC_321|Compound:4534-3890|FD-Score:3.27|P-value:3.16E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.11|P-value:5.76E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.49|P-value:1.34E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.05|P-value:8.24E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:4.31|P-value:3.30E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.08|P-value:6.32E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-3.18|P-value:5.08E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.07|P-value:6.60E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.09|P-value:6.21E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:-4.05|P-value:1.38E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.17|P-value:5.34E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.06|P-value:7.87E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.20|P-value:4.01E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.35|P-value:2.64E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:5.72|P-value:1.23E-9 ID:SGTC_1530|Compound:rhein|FD-Score:3.90|P-value:2.26E-5 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:3.49|P-value:1.34E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:-3.16|P-value:5.58E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.78|P-value:3.92E-5 ID:SGTC_1719|Compound:st032227|FD-Score:-3.53|P-value:1.32E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.02|P-value:7.87E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.06|P-value:7.88E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.23|P-value:3.63E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-5.93|P-value:4.11E-10 ID:SGTC_2275|Compound:7947463|FD-Score:5.01|P-value:8.30E-8 ID:SGTC_2279|Compound:7948423|FD-Score:3.86|P-value:2.79E-5 ID:SGTC_2332|Compound:7982454|FD-Score:-3.90|P-value:2.71E-5 ID:SGTC_2335|Compound:9018011|FD-Score:3.04|P-value:7.28E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.32|P-value:2.58E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-3.01|P-value:9.51E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.76|P-value:4.18E-5 ID:SGTC_2414|Compound:st077232|FD-Score:3.02|P-value:8.00E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.72|P-value:4.95E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.90|P-value:2.81E-5 ID:SGTC_2427|Compound:5545055|FD-Score:-4.79|P-value:3.55E-7 ID:SGTC_2487|Compound:5918373|FD-Score:2.99|P-value:8.77E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.14|P-value:5.98E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:4.17|P-value:6.49E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-4.07|P-value:1.26E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.20|P-value:4.79E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.23|P-value:5.99E-6 ID:SGTC_2865|Compound:9030313|FD-Score:-4.24|P-value:5.68E-6 ID:SGTC_2990|Compound:9074105|FD-Score:-3.10|P-value:6.90E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_3016|Compound:9080866|FD-Score:4.42|P-value:1.98E-6 ID:SGTC_3099|Compound:9120367|FD-Score:-3.23|P-value:4.26E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.28|P-value:3.52E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.10|P-value:5.95E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-3.04|P-value:8.62E-4 ID:SGTC_3195|Compound:9113082|FD-Score:3.05|P-value:7.11E-4 ID:SGTC_321|Compound:4534-3890|FD-Score:3.27|P-value:3.16E-4 ID:SGTC_358|Compound:0075-0058|FD-Score:3.11|P-value:5.76E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.49|P-value:1.34E-4 ID:SGTC_413|Compound:propylparaben|FD-Score:-3.05|P-value:8.24E-4 ID:SGTC_418|Compound:lividomycin a|FD-Score:4.31|P-value:3.30E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:3.08|P-value:6.32E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:-3.18|P-value:5.08E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.07|P-value:6.60E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:3.09|P-value:6.21E-4 ID:SGTC_1011|Compound:4112-3381|FD-Score:-4.05|P-value:1.38E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:-3.17|P-value:5.34E-4 ID:SGTC_1409|Compound:3909-8004|FD-Score:-3.06|P-value:7.87E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.20|P-value:4.01E-4 ID:SGTC_1517|Compound:deoxycholic acid methyl ester|FD-Score:-3.35|P-value:2.64E-4 ID:SGTC_1525|Compound:8-methoxypsoralen|FD-Score:5.72|P-value:1.23E-9 ID:SGTC_1530|Compound:rhein|FD-Score:3.90|P-value:2.26E-5 ID:SGTC_1586|Compound:deoxycorticosterone acetate|FD-Score:3.49|P-value:1.34E-4 ID:SGTC_1595|Compound:6-ethoxy-3(4'-hydroxyphenyl)-4-methylcoumarin|FD-Score:-3.16|P-value:5.58E-4 ID:SGTC_1717|Compound:st033143|FD-Score:3.78|P-value:3.92E-5 ID:SGTC_1719|Compound:st032227|FD-Score:-3.53|P-value:1.32E-4 ID:SGTC_1725|Compound:st036284|FD-Score:3.02|P-value:7.87E-4 ID:SGTC_1943|Compound:st076331|FD-Score:-3.06|P-value:7.88E-4 ID:SGTC_2126|Compound:5251174|FD-Score:3.23|P-value:3.63E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-5.93|P-value:4.11E-10 ID:SGTC_2275|Compound:7947463|FD-Score:5.01|P-value:8.30E-8 ID:SGTC_2279|Compound:7948423|FD-Score:3.86|P-value:2.79E-5 ID:SGTC_2332|Compound:7982454|FD-Score:-3.90|P-value:2.71E-5 ID:SGTC_2335|Compound:9018011|FD-Score:3.04|P-value:7.28E-4 ID:SGTC_2336|Compound:9001819|FD-Score:3.32|P-value:2.58E-4 ID:SGTC_2339|Compound:9000433|FD-Score:-3.01|P-value:9.51E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:3.76|P-value:4.18E-5 ID:SGTC_2414|Compound:st077232|FD-Score:3.02|P-value:8.00E-4 ID:SGTC_2415|Compound:st077333|FD-Score:3.72|P-value:4.95E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:-3.90|P-value:2.81E-5 ID:SGTC_2427|Compound:5545055|FD-Score:-4.79|P-value:3.55E-7 ID:SGTC_2487|Compound:5918373|FD-Score:2.99|P-value:8.77E-4 ID:SGTC_2513|Compound:baicalein|FD-Score:-3.14|P-value:5.98E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:4.17|P-value:6.49E-6 ID:SGTC_2577|Compound:2',4'-dihydroxy-4-methoxychalcone|FD-Score:-4.07|P-value:1.26E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:-3.20|P-value:4.79E-4 ID:SGTC_2626|Compound:ethyl orsellinate|FD-Score:-4.23|P-value:5.99E-6 ID:SGTC_2865|Compound:9030313|FD-Score:-4.24|P-value:5.68E-6 ID:SGTC_2990|Compound:9074105|FD-Score:-3.10|P-value:6.90E-4 ID:SGTC_2991|Compound:9074669|FD-Score:3.15|P-value:5.00E-4 ID:SGTC_3016|Compound:9080866|FD-Score:4.42|P-value:1.98E-6 ID:SGTC_3099|Compound:9120367|FD-Score:-3.23|P-value:4.26E-4 ID:SGTC_3126|Compound:9127922|FD-Score:-3.28|P-value:3.52E-4 ID:SGTC_3135|Compound:9127742|FD-Score:3.10|P-value:5.95E-4 ID:SGTC_3138|Compound:9097216|FD-Score:-3.04|P-value:8.62E-4 ID:SGTC_3195|Compound:9113082|FD-Score:3.05|P-value:7.11E-4

Top fitness defect scores for YGR254W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1525 8-methoxypsoralen 5.72 92.5 µM 1.23E-9 DNA damage response 12 5.43E-9
2 SGTC_2275 7947463 5.01 200.0 µM 8.30E-8 iron homeostasis 22 2.65E-7
3 SGTC_3016 9080866 4.42 49.5 µM 1.98E-6 18 4.97E-6
4 SGTC_418 lividomycin a 4.31 100.0 µM 3.30E-6 7 7.98E-6
5 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 4.17 63.6 µM 6.49E-6 34 1.49E-5
6 SGTC_1530 rhein 3.90 70.4 µM 2.26E-5 20 4.73E-5
7 SGTC_2279 7948423 3.86 32.9 µM 2.79E-5 15 5.74E-5
8 SGTC_1717 st033143 3.78 58.0 µM 3.92E-5 46 7.88E-5
9 SGTC_2405 ticlatone 3.76 3.5 µM 4.18E-5 72 8.36E-5
10 SGTC_2415 st077333 3.72 57.7 µM 4.95E-5 57 9.77E-5
11 SGTC_1586 deoxycorticosterone acetate 3.49 53.7 µM 1.34E-4 24 2.45E-4
12 SGTC_397 arbidol 3.49 50.0 µM 1.34E-4 cell wall 34 2.46E-4
13 SGTC_2336 9001819 3.32 198.7 µM 2.58E-4 heme biosynthesis & mitochondrial translocase 78 4.49E-4
14 SGTC_321 4534-3890 3.27 65.3 µM 3.16E-4 RPP1 & pyrimidine depletion 17 5.43E-4
15 SGTC_2126 5251174 3.23 33.9 µM 3.63E-4 71 6.16E-4
16 SGTC_1510 1123-0200 3.20 71.5 µM 4.01E-4 77 6.76E-4
17 SGTC_2991 9074669 3.15 71.4 µM 5.00E-4 azole & statin 111 8.30E-4
18 SGTC_358 0075-0058 3.11 9.4 µM 5.76E-4 118 9.46E-4
19 SGTC_3135 9127742 3.10 49.5 µM 5.95E-4 49 9.75E-4
20 SGTC_551 k048-0028 3.09 34.9 µM 6.21E-4 54 0.00101

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.127 1.75E-13 YOR282W_d YOR282W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps essential, verified gene PLP2/YOR281C
0.123 7.07E-13 YGR161W-C_p YGR161W-C_p hom Putative protein of unknown function; identified by sequence comparison with hemiascomycetous yeast species
0.105 1.05E-9 YLR401C DUS3 hom Dihydrouridine synthase; member of a widespread family of conserved proteins including Smm1p, Dus1p, and Dus4p; contains a consensus oleate response element (ORE) in its promoter region; forms nuclear foci upon DNA replication stress
0.102 3.32E-9 YOL083W ATG34 hom Receptor protein involved in selective autophagy during starvation; specifically involved in the transport of cargo protein alpha-mannosidase (Ams1p); Atg19p paralog
0.102 3.63E-9 YAL046C AIM1 hom Protein involved in mitochondrial function or organization; null mutant displays elevated frequency of mitochondrial genome loss
0.100 6.96E-9 YHR065C RRP3 het Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity
0.099 8.56E-9 YLR390W-A CCW14 hom Covalently linked cell wall glycoprotein, present in the inner layer of the cell wall
0.098 1.36E-8 YEL020C_p YEL020C_p hom Protein of unknown function with low sequence identity to Pdc1p; mRNA identified as translated by ribosome profiling data
0.094 5.73E-8 YGL255W ZRT1 hom High-affinity zinc transporter of the plasma membrane, responsible for the majority of zinc uptake; transcription is induced under low-zinc conditions by the Zap1p transcription factor
0.089 2.45E-7 YHL007C STE20 hom Cdc42p-activated signal transducing kinase of the PAK (p21-activated kinase) family; involved in pheromone response, pseudohyphal/invasive growth, vacuole inheritance, down-regulation of sterol uptake; GBB motif binds Ste4p
0.089 2.67E-7 YKL046C DCW1 hom Putative mannosidase, GPI-anchored membrane protein required for cell wall biosynthesis in bud formation;homologous to Dfg5p
0.088 2.86E-7 YER067W RGI1 hom Protein of unknown function; involved in energy metabolism under respiratory conditions; protein abundance is increased upon intracellular iron depletion; protein abundance increases in response to DNA replication stress
0.084 1.03E-6 YMR105C PGM2 hom Phosphoglucomutase; catalyzes the conversion from glucose-1-phosphate to glucose-6-phosphate, which is a key step in hexose metabolism; functions as the acceptor for a Glc-phosphotransferase; protein abundance increases in response to DNA replication stress
0.081 2.96E-6 YER093C-A AIM11 hom Protein of unknown function; null mutant is viable but shows increased loss of mitochondrial genome and synthetic interaction with prohibitin (phb1); contains an intron; YER093C-A has a paralog, YBL059W, that arose from the whole genome duplication
0.080 3.49E-6 YKL090W CUE2 hom Protein of unknown function; has two CUE domains that bind ubiquitin, which may facilitate intramolecular monoubiquitination