YGR264C / MES1

Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR264C / MES1

Click on Significant Values for Screen Details ID:SGTC_67|Compound:0335-0847|FD-Score:-3.56|P-value:2.53E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.21|P-value:6.95E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.19|P-value:9.41E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:3.66|P-value:1.36E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:4.03|P-value:3.06E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.73|P-value:1.35E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:5.30|P-value:7.22E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.14|P-value:8.71E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.42|P-value:3.37E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-4.06|P-value:3.63E-5 ID:SGTC_1630|Compound:st005209|FD-Score:-3.41|P-value:4.42E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-3.83|P-value:9.28E-5 ID:SGTC_1642|Compound:st009582|FD-Score:-3.24|P-value:7.83E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.51|P-value:2.38E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.85|P-value:6.52E-5 ID:SGTC_1820|Compound:st053765|FD-Score:6.82|P-value:7.30E-12 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.33|P-value:5.69E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.53|P-value:2.20E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-6.26|P-value:4.22E-10 ID:SGTC_2317|Compound:5986382|FD-Score:3.59|P-value:1.77E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.25|P-value:6.07E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.63|P-value:2.11E-6 ID:SGTC_2415|Compound:st077333|FD-Score:3.42|P-value:3.36E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-4.02|P-value:4.34E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.10|P-value:2.28E-5 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.86|P-value:6.25E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.51|P-value:3.07E-4 ID:SGTC_2731|Compound:amoxapine|FD-Score:4.82|P-value:8.49E-7 ID:SGTC_2911|Compound:7942208|FD-Score:-4.53|P-value:4.69E-6 ID:SGTC_2947|Compound:9072639|FD-Score:-4.71|P-value:1.99E-6 ID:SGTC_2949|Compound:9072542|FD-Score:-4.15|P-value:2.51E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-3.95|P-value:5.66E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.54|P-value:2.77E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-3.43|P-value:4.09E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.59|P-value:2.32E-4 ID:SGTC_3257|Compound:9139206|FD-Score:5.67|P-value:9.65E-9 ID:SGTC_3283|Compound:9109332|FD-Score:-3.29|P-value:6.61E-4 ID:SGTC_3293|Compound:9120939|FD-Score:3.34|P-value:4.40E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:-3.56|P-value:2.53E-4 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:3.21|P-value:6.95E-4 ID:SGTC_519|Compound:k048-0011|FD-Score:-3.19|P-value:9.41E-4 ID:SGTC_521|Compound:r062-0005|FD-Score:3.66|P-value:1.36E-4 ID:SGTC_693|Compound:4161-2696|FD-Score:4.03|P-value:3.06E-5 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.73|P-value:1.35E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:5.30|P-value:7.22E-8 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.14|P-value:8.71E-4 ID:SGTC_1180|Compound:1193-0155|FD-Score:3.42|P-value:3.37E-4 ID:SGTC_1523|Compound:st012844|FD-Score:-4.06|P-value:3.63E-5 ID:SGTC_1630|Compound:st005209|FD-Score:-3.41|P-value:4.42E-4 ID:SGTC_1633|Compound:st005664|FD-Score:-3.83|P-value:9.28E-5 ID:SGTC_1642|Compound:st009582|FD-Score:-3.24|P-value:7.83E-4 ID:SGTC_1680|Compound:diphenhydramine|FD-Score:3.51|P-value:2.38E-4 ID:SGTC_1814|Compound:st052110|FD-Score:3.85|P-value:6.52E-5 ID:SGTC_1820|Compound:st053765|FD-Score:6.82|P-value:7.30E-12 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:-3.33|P-value:5.69E-4 ID:SGTC_2259|Compound:7964009|FD-Score:3.53|P-value:2.20E-4 ID:SGTC_2282|Compound:7944268|FD-Score:-6.26|P-value:4.22E-10 ID:SGTC_2317|Compound:5986382|FD-Score:3.59|P-value:1.77E-4 ID:SGTC_2337|Compound:9009472|FD-Score:3.25|P-value:6.07E-4 ID:SGTC_2406|Compound:ethylparaben|FD-Score:4.63|P-value:2.11E-6 ID:SGTC_2415|Compound:st077333|FD-Score:3.42|P-value:3.36E-4 ID:SGTC_2433|Compound:5607874|FD-Score:-4.02|P-value:4.34E-5 ID:SGTC_2609|Compound:2,3,4-trihydroxy-4'-ethoxybenzophenone|FD-Score:4.10|P-value:2.28E-5 ID:SGTC_2629|Compound:bisabolol|FD-Score:3.86|P-value:6.25E-5 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-3.51|P-value:3.07E-4 ID:SGTC_2731|Compound:amoxapine|FD-Score:4.82|P-value:8.49E-7 ID:SGTC_2911|Compound:7942208|FD-Score:-4.53|P-value:4.69E-6 ID:SGTC_2947|Compound:9072639|FD-Score:-4.71|P-value:1.99E-6 ID:SGTC_2949|Compound:9072542|FD-Score:-4.15|P-value:2.51E-5 ID:SGTC_2951|Compound:9072950|FD-Score:-3.95|P-value:5.66E-5 ID:SGTC_2993|Compound:9054257|FD-Score:-3.54|P-value:2.77E-4 ID:SGTC_3140|Compound:9094243|FD-Score:-3.43|P-value:4.09E-4 ID:SGTC_3170|Compound:9103054|FD-Score:-3.59|P-value:2.32E-4 ID:SGTC_3257|Compound:9139206|FD-Score:5.67|P-value:9.65E-9 ID:SGTC_3283|Compound:9109332|FD-Score:-3.29|P-value:6.61E-4 ID:SGTC_3293|Compound:9120939|FD-Score:3.34|P-value:4.40E-4

Top fitness defect scores for YGR264C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1820 st053765 6.82 18.6 µM 7.30E-12 RPP1 & pyrimidine depletion 2 4.67E-12 1.21
2 SGTC_3257 9139206 5.67 33.0 µM 9.65E-9 1 7.31E-9 0.83
3 SGTC_1013 4112-3890 5.30 241.0 µM 7.22E-8 TSC3-RPN4 2 5.72E-8 0.54
4 SGTC_2731 amoxapine 4.82 43.4 µM 8.49E-7 1 7.10E-7 1.02
5 SGTC_2406 ethylparaben 4.63 405.7 µM 2.11E-6 7 1.80E-6 0.49
6 SGTC_2609 2,3,4-trihydroxy-4'-ethoxybenzophenone 4.10 70.0 µM 2.28E-5 redox potentiating 8 2.04E-5 0.03
7 SGTC_693 4161-2696 4.03 114.0 µM 3.06E-5 3 2.76E-5 0.13
8 SGTC_2629 bisabolol 3.86 100.0 µM 6.25E-5 9 5.71E-5 0.05
9 SGTC_1814 st052110 3.85 31.5 µM 6.52E-5 RNA processing & uracil transport 9 5.96E-5 0.10
10 SGTC_521 r062-0005 3.66 13.8 µM 1.36E-4 3 1.26E-4 0.04
11 SGTC_2317 5986382 3.59 200.0 µM 1.77E-4 7 1.65E-4 0.09
12 SGTC_2259 7964009 3.53 200.0 µM 2.20E-4 superoxide 7 2.06E-4 0.14
13 SGTC_1680 diphenhydramine 3.51 100.0 µM 2.38E-4 NEO1 19 2.24E-4 0.16
14 SGTC_2415 st077333 3.42 57.7 µM 3.36E-4 21 3.17E-4 0.03
15 SGTC_1180 1193-0155 3.42 285.0 µM 3.37E-4 5 3.18E-4 0.14
16 SGTC_3293 9120939 3.34 62.0 µM 4.40E-4 16 4.17E-4 0.03
17 SGTC_2337 9009472 3.25 200.0 µM 6.07E-4 16 5.80E-4 0.04
18 SGTC_506 n9-isopropylolomoucine 3.21 153.0 µM 6.95E-4 6 6.65E-4 0.08
19 SGTC_1130 3454-2858 3.14 15.9 µM 8.71E-4 NEO1 21 8.36E-4 0.07
20 SGTC_1170 0310-0127 3.08 64.8 µM 0.00107 25 0.00103 0.03

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.124 4.81E-13 YLR026C SED5 het cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.119 4.99E-12 YAL034C-B_d YAL034C-B_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.104 1.57E-9 YPR025C CCL1 het Cyclin associated with protein kinase Kin28p, which is the TFIIH-associated carboxy-terminal domain (CTD) kinase involved in transcription initiation at RNA polymerase II promoters
0.099 7.69E-9 YDL008W APC11 het Catalytic core subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition; contains a RING-H2 domain that is required for activity
0.091 1.22E-7 YDR471W RPL27B hom Ribosomal 60S subunit protein L27B; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27B has a paralog, RPL27A, that arose from the whole genome duplication
0.091 1.35E-7 YNL203C_d YNL203C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.089 2.15E-7 YNL097C-B_p YNL097C-B_p hom Putative protein of unknown function
0.088 2.96E-7 YDL012C YDL012C hom Tail-anchored plasma membrane protein with a conserved CYSTM module; possibly involved in response to stress; may contribute to non-homologous end-joining (NHEJ) based on ydl012c htz1 double null phenotype; YDL012C has a paralog, YBR016W, that arose from the whole genome duplication
0.087 4.18E-7 YPL218W SAR1 het GTPase, GTP-binding protein of the ARF family, component of COPII coat of vesicles; required for transport vesicle formation during ER to Golgi protein transport
0.084 1.19E-6 YKL179C COY1 hom Golgi membrane protein with similarity to mammalian CASP; genetic interactions with GOS1 (encoding a Golgi snare protein) suggest a role in Golgi function
0.083 1.54E-6 YBR274W CHK1 hom Serine/threonine kinase and DNA damage checkpoint effector, mediates cell cycle arrest via phosphorylation of Pds1p; phosphorylated by checkpoint signal transducer Mec1p; homolog of S. pombe and mammalian Chk1 checkpoint kinase
0.082 2.12E-6 YLR100W ERG27 het 3-keto sterol reductase, catalyzes the last of three steps required to remove two C-4 methyl groups from an intermediate in ergosterol biosynthesis; mutants are sterol auxotrophs
0.079 5.13E-6 YGL108C YGL108C hom Protein of unknown function, predicted to be palmitoylated; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery; protein abundance increases in response to DNA replication stress
0.077 7.02E-6 YNR071C_p YNR071C_p hom Putative aldose 1-epimerase
0.075 1.35E-5 YKL220C FRE2 hom Ferric reductase and cupric reductase, reduces siderophore-bound iron and oxidized copper prior to uptake by transporters; expression induced by low iron levels but not by low copper levels