YGR274C / TAF1

TFIID subunit (145 kDa), involved in RNA polymerase II transcription initiation; possesses in vitro histone acetyltransferase activity but its role in vivo appears to be minor; involved in promoter binding and G1/S progression

Zygosity: Heterozygous strain
fixedexpanded
Profile for YGR274C / TAF1

Click on Significant Values for Screen Details ID:SGTC_162|Compound:k030-0015|FD-Score:3.33|P-value:6.11E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.69|P-value:1.87E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:4.54|P-value:5.27E-6 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.14|P-value:3.34E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:-4.03|P-value:5.11E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.39|P-value:4.92E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.88|P-value:8.27E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.14|P-value:2.91E-5 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.49|P-value:3.51E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.34|P-value:5.95E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.23|P-value:8.48E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.26|P-value:7.85E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.64|P-value:2.02E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.55|P-value:2.83E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.72|P-value:1.55E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.53|P-value:3.08E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.37|P-value:5.38E-4 ID:SGTC_3067|Compound:9117431|FD-Score:4.04|P-value:4.48E-5 ID:SGTC_3076|Compound:9116187|FD-Score:-3.36|P-value:6.14E-4 ID:SGTC_162|Compound:k030-0015|FD-Score:3.33|P-value:6.11E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-3.69|P-value:1.87E-4 ID:SGTC_363|Compound:0455-0021|FD-Score:4.54|P-value:5.27E-6 ID:SGTC_371|Compound:0195-0006|FD-Score:-4.14|P-value:3.34E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:-4.03|P-value:5.11E-5 ID:SGTC_1100|Compound:amiodarone|FD-Score:3.39|P-value:4.92E-4 ID:SGTC_1189|Compound:1431-2119|FD-Score:3.88|P-value:8.27E-5 ID:SGTC_1214|Compound:0358-0031|FD-Score:4.14|P-value:2.91E-5 ID:SGTC_1437|Compound:4092-1038|FD-Score:3.49|P-value:3.51E-4 ID:SGTC_1752|Compound:st044819|FD-Score:3.34|P-value:5.95E-4 ID:SGTC_1782|Compound:st039423|FD-Score:3.23|P-value:8.48E-4 ID:SGTC_2012|Compound:4023501|FD-Score:3.26|P-value:7.85E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.64|P-value:2.02E-4 ID:SGTC_2425|Compound:5628681|FD-Score:3.55|P-value:2.83E-4 ID:SGTC_2453|Compound:5731132|FD-Score:3.72|P-value:1.55E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.53|P-value:3.08E-4 ID:SGTC_2572|Compound:astragaloside iv|FD-Score:3.37|P-value:5.38E-4 ID:SGTC_3067|Compound:9117431|FD-Score:4.04|P-value:4.48E-5 ID:SGTC_3076|Compound:9116187|FD-Score:-3.36|P-value:6.14E-4

Top fitness defect scores for YGR274C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_363 0455-0021 4.54 3.1 µM 5.27E-6 azole & statin 15 2.78E-6 0.30
2 SGTC_1214 0358-0031 4.14 63.7 µM 2.91E-5 5 1.71E-5 0.10
3 SGTC_3067 9117431 4.04 49.5 µM 4.48E-5 7 2.71E-5 0.08
4 SGTC_1189 1431-2119 3.88 7.3 µM 8.27E-5 10 5.19E-5 0.03
5 SGTC_2453 5731132 3.72 22.2 µM 1.55E-4 6 1.01E-4 0.01
6 SGTC_2424 5537928 3.64 59.2 µM 2.02E-4 11 1.34E-4 0.01
7 SGTC_2425 5628681 3.55 7.8 µM 2.83E-4 8 1.92E-4 0.14
8 SGTC_2566 crystal violet carbinol 3.53 226.0 nM 3.08E-4 26 2.09E-4 0.03
9 SGTC_1437 4092-1038 3.49 11.6 µM 3.51E-4 7 2.41E-4 0.23
10 SGTC_1100 amiodarone 3.39 5.1 µM 4.92E-4 6 3.45E-4 0.23
11 SGTC_2572 astragaloside iv 3.37 100.0 µM 5.38E-4 13 3.79E-4 0.07
12 SGTC_1752 st044819 3.34 83.0 µM 5.95E-4 19 4.21E-4 0.01
13 SGTC_162 k030-0015 3.33 37.8 µM 6.11E-4 iron homeostasis 6 4.33E-4 0.02
14 SGTC_2012 4023501 3.26 200.0 µM 7.85E-4 20 5.66E-4 0.07
15 SGTC_1782 st039423 3.23 80.6 µM 8.48E-4 8 6.14E-4 0.20
16 SGTC_1957 st077035 3.16 62.4 µM 0.00107 4 7.84E-4 0.18
17 SGTC_1464 k208-0027 3.14 110.0 µM 0.00113 11 8.31E-4 0.17
18 SGTC_1852 st056254 3.10 31.4 µM 0.00130 5 9.67E-4 0.22
19 SGTC_1070 indatraline 3.02 8.8 µM 0.00169 60S ribosome export 15 0.00128 0.03
20 SGTC_1619 st003326 3.02 88.7 µM 0.00170 60S ribosome export 10 0.00128 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.129 7.02E-14 YKR034W DAL80 hom Negative regulator of genes in multiple nitrogen degradation pathways; expression is regulated by nitrogen levels and by Gln3p; member of the GATA-binding family, forms homodimers and heterodimers with Gzf3p; DAL80 has a paralog, GZF3, that arose from the whole genome duplication
0.119 4.76E-12 YGL262W_p YGL262W_p hom Putative protein of unknown function; null mutant displays elevated sensitivity to expression of a mutant huntingtin fragment or of alpha-synuclein; YGL262W is not an essential gene
0.117 1.15E-11 YOL150C_d YOL150C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.114 4.04E-11 YPL061W ALD6 hom Cytosolic aldehyde dehydrogenase, activated by Mg2+ and utilizes NADP+ as the preferred coenzyme; required for conversion of acetaldehyde to acetate; constitutively expressed; locates to the mitochondrial outer surface upon oxidative stress
0.112 7.84E-11 YGR170W PSD2 hom Phosphatidylserine decarboxylase of the Golgi and vacuolar membranes, converts phosphatidylserine to phosphatidylethanolamine
0.104 1.52E-9 YKL104C GFA1 het Glutamine-fructose-6-phosphate amidotransferase; catalyzes the formation of glucosamine-6-P and glutamate from fructose-6-P and glutamine in the first step of chitin biosynthesis; GFA1 has a paralogous region, comprising ORFs YMR084W-YMR085W, that arose from the whole genome duplication
0.104 1.66E-9 YBR121C GRS1 het Cytoplasmic and mitochondrial glycyl-tRNA synthase; ligates glycine to the cognate anticodon-bearing tRNA; transcription termination factor that may interact with the 3'-end of pre-mRNA to promote 3'-end formation; GRS1 has a paralog, GRS2, that arose from the whole genome duplication
0.103 2.37E-9 YHR130C_d YHR130C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.103 2.51E-9 YLR364W GRX8 hom Glutaredoxin that employs a dithiol mechanism of catalysis; monomeric; activity is low and null mutation does not affect sensitivity to oxidative stress; GFP-fusion protein localizes to the cytoplasm; expression strongly induced by arsenic
0.091 1.33E-7 YPL062W_d YPL062W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YPL062W is not an essential gene; homozygous diploid mutant shows a decrease in glycogen accumulation
0.087 4.69E-7 YGR004W PEX31 hom Peroxisomal integral membrane protein, involved in negative regulation of peroxisome size; partially functionally redundant with Pex30p and Pex32p; probably acts at a step downstream of steps mediated by Pex28p and Pex29p
0.083 1.41E-6 YHR126C_p ANS1_p hom Putative GPI protein; transcription dependent upon Azf1p
0.082 2.22E-6 YJR019C TES1 hom Peroxisomal acyl-CoA thioesterase likely to be involved in fatty acid oxidation rather than fatty acid synthesis; conserved protein also found in human peroxisomes; TES1 mRNA levels increase during growth on fatty acids
0.082 2.28E-6 YER053C-A YER053C-A hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the endoplasmic reticulum; protein abundance increases in response to DNA replication stress
0.081 2.47E-6 YAR050W FLO1 hom Lectin-like protein involved in flocculation, cell wall protein that binds to mannose chains on the surface of other cells, confers floc-forming ability that is chymotrypsin sensitive and heat resistant; similar to Flo5p