YHL002W / HSE1

Subunit of the endosomal Vps27p-Hse1p complex required for sorting of ubiquitinated membrane proteins into intralumenal vesicles prior to vacuolar degradation, as well as for recycling of Golgi proteins and formation of lumenal membranes

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL002W / HSE1

Click on Significant Values for Screen Details ID:SGTC_6|Compound:0844-0013|FD-Score:5.79|P-value:1.78E-8 ID:SGTC_16|Compound:erodoxin|FD-Score:-3.77|P-value:1.65E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.20|P-value:1.76E-9 ID:SGTC_76|Compound:0433-0009|FD-Score:3.67|P-value:2.45E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:-3.31|P-value:8.15E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.69|P-value:4.17E-6 ID:SGTC_308|Compound:4092-1169|FD-Score:3.47|P-value:4.92E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:4.76|P-value:3.05E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.15|P-value:2.34E-9 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.55|P-value:3.63E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.40|P-value:6.04E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.94|P-value:8.83E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:4.61|P-value:5.70E-6 ID:SGTC_1025|Compound:k007-0731|FD-Score:-4.13|P-value:4.29E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:4.57|P-value:6.89E-6 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.70|P-value:2.18E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.51|P-value:8.97E-6 ID:SGTC_1120|Compound:ficusin|FD-Score:4.05|P-value:5.85E-5 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.40|P-value:1.43E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.61|P-value:2.99E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.95|P-value:8.52E-5 ID:SGTC_1687|Compound:st020778|FD-Score:5.63|P-value:4.23E-8 ID:SGTC_1712|Compound:st032288|FD-Score:6.92|P-value:2.32E-11 ID:SGTC_1831|Compound:st055328|FD-Score:3.98|P-value:7.64E-5 ID:SGTC_1842|Compound:st055362|FD-Score:4.77|P-value:2.82E-6 ID:SGTC_1914|Compound:st062367|FD-Score:3.81|P-value:1.47E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-4.27|P-value:2.40E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.26|P-value:9.56E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-3.35|P-value:7.08E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.28|P-value:2.37E-5 ID:SGTC_2086|Compound:5274770|FD-Score:3.37|P-value:6.79E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.27|P-value:9.14E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.74|P-value:1.87E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.56|P-value:7.32E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:5.44|P-value:1.17E-7 ID:SGTC_3024|Compound:9090631|FD-Score:-3.36|P-value:6.96E-4 ID:SGTC_3118|Compound:9124250|FD-Score:-3.50|P-value:4.35E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-3.46|P-value:4.88E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.58|P-value:3.30E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.43|P-value:5.52E-4 ID:SGTC_6|Compound:0844-0013|FD-Score:5.79|P-value:1.78E-8 ID:SGTC_16|Compound:erodoxin|FD-Score:-3.77|P-value:1.65E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:-6.20|P-value:1.76E-9 ID:SGTC_76|Compound:0433-0009|FD-Score:3.67|P-value:2.45E-4 ID:SGTC_87|Compound:0100-0052|FD-Score:-3.31|P-value:8.15E-4 ID:SGTC_131|Compound:0141-0277|FD-Score:4.69|P-value:4.17E-6 ID:SGTC_308|Compound:4092-1169|FD-Score:3.47|P-value:4.92E-4 ID:SGTC_432|Compound:NS 1619|FD-Score:4.76|P-value:3.05E-6 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-6.15|P-value:2.34E-9 ID:SGTC_513|Compound:4245-1575|FD-Score:-3.55|P-value:3.63E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:3.40|P-value:6.04E-4 ID:SGTC_636|Compound:0kpi-0074|FD-Score:3.94|P-value:8.83E-5 ID:SGTC_927|Compound:2425-1185|FD-Score:4.61|P-value:5.70E-6 ID:SGTC_1025|Compound:k007-0731|FD-Score:-4.13|P-value:4.29E-5 ID:SGTC_1030|Compound:k029-0042|FD-Score:4.57|P-value:6.89E-6 ID:SGTC_1033|Compound:k072-0102|FD-Score:3.70|P-value:2.18E-4 ID:SGTC_1042|Compound:0350-0096|FD-Score:4.51|P-value:8.97E-6 ID:SGTC_1120|Compound:ficusin|FD-Score:4.05|P-value:5.85E-5 ID:SGTC_1283|Compound:0922-0125|FD-Score:4.40|P-value:1.43E-5 ID:SGTC_1376|Compound:2910-0861|FD-Score:3.61|P-value:2.99E-4 ID:SGTC_1495|Compound:4469-0029|FD-Score:3.95|P-value:8.52E-5 ID:SGTC_1687|Compound:st020778|FD-Score:5.63|P-value:4.23E-8 ID:SGTC_1712|Compound:st032288|FD-Score:6.92|P-value:2.32E-11 ID:SGTC_1831|Compound:st055328|FD-Score:3.98|P-value:7.64E-5 ID:SGTC_1842|Compound:st055362|FD-Score:4.77|P-value:2.82E-6 ID:SGTC_1914|Compound:st062367|FD-Score:3.81|P-value:1.47E-4 ID:SGTC_1918|Compound:oxolan-2-ylmethyl 3-bromobenzoate|FD-Score:-4.27|P-value:2.40E-5 ID:SGTC_1919|Compound:st067113|FD-Score:3.26|P-value:9.56E-4 ID:SGTC_1938|Compound:st074717|FD-Score:-3.35|P-value:7.08E-4 ID:SGTC_2021|Compound:4019055|FD-Score:4.28|P-value:2.37E-5 ID:SGTC_2086|Compound:5274770|FD-Score:3.37|P-value:6.79E-4 ID:SGTC_2324|Compound:6149169|FD-Score:-3.27|P-value:9.14E-4 ID:SGTC_2494|Compound:18-aminoabieta-8,11,13-triene sulfate|FD-Score:3.74|P-value:1.87E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.56|P-value:7.32E-6 ID:SGTC_2755|Compound:lomerizine|FD-Score:5.44|P-value:1.17E-7 ID:SGTC_3024|Compound:9090631|FD-Score:-3.36|P-value:6.96E-4 ID:SGTC_3118|Compound:9124250|FD-Score:-3.50|P-value:4.35E-4 ID:SGTC_3119|Compound:9125183|FD-Score:-3.46|P-value:4.88E-4 ID:SGTC_3202|Compound:9113761|FD-Score:-3.58|P-value:3.30E-4 ID:SGTC_3205|Compound:9113483|FD-Score:-3.43|P-value:5.52E-4

Top fitness defect scores for YHL002W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1712 st032288 6.92 53.4 µM 2.32E-11 calcium & mitochondrial duress 56 2.25E-12
2 SGTC_6 0844-0013 5.79 19.8 µM 1.78E-8 18 3.43E-9
3 SGTC_1687 st020778 5.63 55.4 µM 4.23E-8 117 8.89E-9
4 SGTC_2755 lomerizine 5.44 42.7 µM 1.17E-7 6 2.72E-8
5 SGTC_1842 st055362 4.77 61.2 µM 2.82E-6 5 9.06E-7
6 SGTC_432 NS 1619 4.76 61.3 µM 3.05E-6 12 9.88E-7
7 SGTC_131 0141-0277 4.69 33.9 µM 4.17E-6 20 1.39E-6
8 SGTC_927 2425-1185 4.61 123.0 µM 5.70E-6 18 1.97E-6
9 SGTC_1030 k029-0042 4.57 36.7 µM 6.89E-6 TSC3-RPN4 13 2.42E-6
10 SGTC_2688 kanamycin b 4.56 96.7 µM 7.32E-6 calcium & mitochondrial duress 290 2.59E-6
11 SGTC_1042 0350-0096 4.51 82.0 µM 8.97E-6 TSC3-RPN4 12 3.24E-6
12 SGTC_1283 0922-0125 4.40 16.4 µM 1.43E-5 14 5.42E-6
13 SGTC_2021 4019055 4.28 173.0 µM 2.37E-5 endomembrane recycling 103 9.46E-6
14 SGTC_1120 ficusin 4.05 1.8 µM 5.85E-5 22 2.55E-5
15 SGTC_1831 st055328 3.98 12.0 µM 7.64E-5 heme biosynthesis & mitochondrial translocase 23 3.42E-5
16 SGTC_1495 4469-0029 3.95 237.0 µM 8.52E-5 107 3.86E-5
17 SGTC_636 0kpi-0074 3.94 126.0 µM 8.83E-5 19 4.01E-5
18 SGTC_1914 st062367 3.81 72.6 µM 1.47E-4 24 7.00E-5
19 SGTC_2494 18-aminoabieta-8,11,13-triene sulfate 3.74 3.7 µM 1.87E-4 102 9.17E-5
20 SGTC_1033 k072-0102 3.70 21.3 µM 2.18E-4 60S ribosome export 30 1.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.230 2.00E-41 YDR466W PKH3 hom Protein kinase with similarity to mammalian phosphoinositide-dependent kinase 1 (PDK1) and yeast Pkh1p and Pkh2p, two redundant upstream activators of Pkc1p; identified as a multicopy suppressor of a pkh1 pkh2 double mutant
0.222 9.87E-39 YDR467C_d YDR467C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.217 5.18E-37 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.215 2.58E-36 YDL019C OSH2 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.210 1.26E-34 YGL203C KEX1 hom Protease involved in the processing of killer toxin and alpha factor precursor; cleaves Lys and Arg residues from the C-terminus of peptides and proteins
0.193 2.15E-29 YGR206W MVB12 hom ESCRT-I subunit required to stabilize oligomers of the ESCRT-I core complex (Stp22p, Vps28p, Srn2p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; deletion mutant is sensitive to rapamycin and nystatin
0.192 2.43E-29 YJL164C TPK1 hom cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; TPK1 has a paralog, TPK3, that arose from the whole genome duplication
0.192 2.79E-29 YGR261C APL6 hom Beta3-like subunit of the yeast AP-3 complex; functions in transport of alkaline phosphatase to the vacuole via the alternate pathway; exists in both cytosolic and peripherally associated membrane-bound pools
0.186 1.45E-27 YDR475C JIP4 hom Protein of unknown function; previously annotated as two separate ORFs, YDR474C and YDR475C, which were merged as a result of corrections to the systematic reference sequence
0.185 3.20E-27 YAL002W VPS8 hom Membrane-binding component of the CORVET complex; involved in endosomal vesicle tethering and fusion in the endosome to vacuole protein targeting pathway; interacts with Vps21p; contains RING finger motif
0.183 1.36E-26 YPL120W VPS30 hom Subunit of phosphatidylinositol (PtdIns) 3-kinase complexes I and II; Complex I is essential in autophagy and Complex II is required for vacuolar protein sorting; required for overflow degradation of misfolded proteins when ERAD is saturated; ortholog of the higher eukaryotic gene Beclin 1
0.171 2.13E-23 YDR357C_p CNL1_p hom Protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; null mutant is sensitive to drug inducing secretion of vacuolar cargo; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.169 6.07E-23 YBL051C PIN4 hom Protein involved in G2/M phase progression and response to DNA damage, interacts with Rad53p; contains an RNA recognition motif, a nuclear localization signal, and several SQ/TQ cluster domains; hyperphosphorylated in response to DNA damage
0.168 8.85E-23 YPL145C KES1 hom One of seven members of the yeast oxysterol binding protein family; involved in negative regulation of Sec14p-dependent Golgi complex secretory functions, peripheral membrane protein that localizes to the Golgi complex; KES1 has a paralog, HES1, that arose from the whole genome duplication
0.167 2.18E-22 YEL022W GEA2 hom Guanine nucleotide exchange factor for ADP ribosylation factors (ARFs); involved in vesicular transport between the Golgi and ER, Golgi organization, and actin cytoskeleton organization; GEA2 has a paralog, GEA1, that arose from the whole genome duplication