YHL011C / PRS3

5-phospho-ribosyl-1(alpha)-pyrophosphate synthetase, synthesizes PRPP, which is required for nucleotide, histidine, and tryptophan biosynthesis; one of five related enzymes, which are active as heteromultimeric complexes

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL011C / PRS3

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:3.87|P-value:1.67E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.45|P-value:7.04E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.37|P-value:9.18E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:3.89|P-value:1.55E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:3.57|P-value:4.69E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.90|P-value:2.80E-6 ID:SGTC_129|Compound:0088-0017|FD-Score:3.56|P-value:4.92E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.36|P-value:9.39E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:5.06|P-value:1.39E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:4.65|P-value:8.21E-6 ID:SGTC_172|Compound:1112-0046|FD-Score:-3.45|P-value:6.69E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.57|P-value:1.16E-12 ID:SGTC_232|Compound:clozapine|FD-Score:4.00|P-value:1.07E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:7.10|P-value:2.36E-11 ID:SGTC_375|Compound:3970-0961|FD-Score:4.47|P-value:1.71E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.96|P-value:1.24E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.06|P-value:1.37E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:3.37|P-value:9.01E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:3.71|P-value:2.98E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.59|P-value:4.45E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.89|P-value:3.02E-6 ID:SGTC_823|Compound:0417-1665|FD-Score:3.82|P-value:2.01E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.71|P-value:3.01E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.81|P-value:2.10E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:4.37|P-value:2.63E-5 ID:SGTC_888|Compound:0916-0002|FD-Score:3.61|P-value:4.17E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:7.35|P-value:4.74E-12 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.78|P-value:2.35E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:7.86|P-value:1.61E-13 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.00|P-value:1.05E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:4.27|P-value:3.84E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.68|P-value:3.31E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:3.88|P-value:1.65E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.61|P-value:4.14E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.86|P-value:3.33E-6 ID:SGTC_1304|Compound:1165-0502|FD-Score:8.65|P-value:5.50E-16 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.36|P-value:9.37E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-4.43|P-value:1.93E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:4.01|P-value:1.03E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:5.20|P-value:7.17E-7 ID:SGTC_1656|Compound:st012921|FD-Score:3.44|P-value:7.33E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.39|P-value:8.17E-4 ID:SGTC_1694|Compound:st024011|FD-Score:4.26|P-value:4.04E-5 ID:SGTC_1781|Compound:st038725|FD-Score:7.05|P-value:3.25E-11 ID:SGTC_1800|Compound:st050079|FD-Score:4.01|P-value:1.02E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.21|P-value:4.75E-5 ID:SGTC_1874|Compound:staurosporine|FD-Score:8.08|P-value:3.38E-14 ID:SGTC_1877|Compound:wortmannin|FD-Score:9.47|P-value:8.35E-19 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.77|P-value:2.32E-4 ID:SGTC_1907|Compound:st060210|FD-Score:5.05|P-value:1.47E-6 ID:SGTC_2041|Compound:5107442|FD-Score:4.35|P-value:2.77E-5 ID:SGTC_2064|Compound:5220758|FD-Score:4.10|P-value:7.37E-5 ID:SGTC_2068|Compound:5226020|FD-Score:-3.64|P-value:3.58E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.67|P-value:7.65E-6 ID:SGTC_2132|Compound:5317594|FD-Score:7.35|P-value:4.78E-12 ID:SGTC_2137|Compound:5261020|FD-Score:4.39|P-value:2.37E-5 ID:SGTC_2150|Compound:5556613|FD-Score:4.96|P-value:2.18E-6 ID:SGTC_2233|Compound:sulbentine|FD-Score:5.02|P-value:1.69E-6 ID:SGTC_2239|Compound:6685329|FD-Score:5.36|P-value:3.42E-7 ID:SGTC_2284|Compound:7945075|FD-Score:-4.03|P-value:9.13E-5 ID:SGTC_2290|Compound:7904846|FD-Score:3.41|P-value:7.89E-4 ID:SGTC_2322|Compound:7413319|FD-Score:7.42|P-value:2.96E-12 ID:SGTC_2345|Compound:9052315|FD-Score:4.42|P-value:2.12E-5 ID:SGTC_2430|Compound:5571206|FD-Score:7.03|P-value:3.59E-11 ID:SGTC_2469|Compound:5655019|FD-Score:4.29|P-value:3.58E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.13|P-value:6.48E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.45|P-value:7.10E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:7.13|P-value:1.94E-11 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.36|P-value:2.74E-5 ID:SGTC_2806|Compound:7952793|FD-Score:3.82|P-value:2.02E-4 ID:SGTC_2932|Compound:9016711|FD-Score:5.78|P-value:4.27E-8 ID:SGTC_2976|Compound:9009005|FD-Score:5.74|P-value:5.13E-8 ID:SGTC_3059|Compound:1327-0034|FD-Score:5.54|P-value:1.45E-7 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.65|P-value:3.69E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:6.03|P-value:1.14E-8 ID:SGTC_3093|Compound:9117344|FD-Score:4.22|P-value:4.68E-5 ID:SGTC_3124|Compound:9127567|FD-Score:3.37|P-value:9.03E-4 ID:SGTC_3237|Compound:9133362|FD-Score:4.13|P-value:6.57E-5 ID:SGTC_3303|Compound:9125618|FD-Score:5.47|P-value:2.03E-7 ID:SGTC_18|Compound:0111-0177|FD-Score:3.87|P-value:1.67E-4 ID:SGTC_34|Compound:0469-0683|FD-Score:3.45|P-value:7.04E-4 ID:SGTC_55|Compound:0767-0285|FD-Score:3.37|P-value:9.18E-4 ID:SGTC_59|Compound:0330-0160|FD-Score:3.89|P-value:1.55E-4 ID:SGTC_90|Compound:0870-0863|FD-Score:3.57|P-value:4.69E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.90|P-value:2.80E-6 ID:SGTC_129|Compound:0088-0017|FD-Score:3.56|P-value:4.92E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:3.36|P-value:9.39E-4 ID:SGTC_161|Compound:4065-0411|FD-Score:5.06|P-value:1.39E-6 ID:SGTC_163|Compound:1327-0036|FD-Score:4.65|P-value:8.21E-6 ID:SGTC_172|Compound:1112-0046|FD-Score:-3.45|P-value:6.69E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:7.57|P-value:1.16E-12 ID:SGTC_232|Compound:clozapine|FD-Score:4.00|P-value:1.07E-4 ID:SGTC_337|Compound:1315-0038|FD-Score:7.10|P-value:2.36E-11 ID:SGTC_375|Compound:3970-0961|FD-Score:4.47|P-value:1.71E-5 ID:SGTC_440|Compound:fluspirilene|FD-Score:3.96|P-value:1.24E-4 ID:SGTC_445|Compound:e6 berbamine|FD-Score:5.06|P-value:1.37E-6 ID:SGTC_528|Compound:3492-0019|FD-Score:3.37|P-value:9.01E-4 ID:SGTC_581|Compound:0958-0271|FD-Score:3.71|P-value:2.98E-4 ID:SGTC_592|Compound:k241-0073|FD-Score:3.59|P-value:4.45E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:4.89|P-value:3.02E-6 ID:SGTC_823|Compound:0417-1665|FD-Score:3.82|P-value:2.01E-4 ID:SGTC_830|Compound:4076-0286|FD-Score:3.71|P-value:3.01E-4 ID:SGTC_831|Compound:k061-1017|FD-Score:3.81|P-value:2.10E-4 ID:SGTC_845|Compound:0330-0138|FD-Score:4.37|P-value:2.63E-5 ID:SGTC_888|Compound:0916-0002|FD-Score:3.61|P-value:4.17E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:7.35|P-value:4.74E-12 ID:SGTC_1018|Compound:4259-0013|FD-Score:3.78|P-value:2.35E-4 ID:SGTC_1154|Compound:4088-0362|FD-Score:7.86|P-value:1.61E-13 ID:SGTC_1173|Compound:0443-0020|FD-Score:4.00|P-value:1.05E-4 ID:SGTC_1199|Compound:1638-0010|FD-Score:4.27|P-value:3.84E-5 ID:SGTC_1237|Compound:0330-0133|FD-Score:3.68|P-value:3.31E-4 ID:SGTC_1240|Compound:0356-0016|FD-Score:3.88|P-value:1.65E-4 ID:SGTC_1242|Compound:0402-0103|FD-Score:3.61|P-value:4.14E-4 ID:SGTC_1256|Compound:0570-0116|FD-Score:4.86|P-value:3.33E-6 ID:SGTC_1304|Compound:1165-0502|FD-Score:8.65|P-value:5.50E-16 ID:SGTC_1309|Compound:pyrazolanthrone|FD-Score:3.36|P-value:9.37E-4 ID:SGTC_1522|Compound:st012842|FD-Score:-4.43|P-value:1.93E-5 ID:SGTC_1545|Compound:lovastatin|FD-Score:4.01|P-value:1.03E-4 ID:SGTC_1562|Compound:morin hydrate|FD-Score:5.20|P-value:7.17E-7 ID:SGTC_1656|Compound:st012921|FD-Score:3.44|P-value:7.33E-4 ID:SGTC_1672|Compound:st016598|FD-Score:-3.39|P-value:8.17E-4 ID:SGTC_1694|Compound:st024011|FD-Score:4.26|P-value:4.04E-5 ID:SGTC_1781|Compound:st038725|FD-Score:7.05|P-value:3.25E-11 ID:SGTC_1800|Compound:st050079|FD-Score:4.01|P-value:1.02E-4 ID:SGTC_1803|Compound:st050068|FD-Score:4.21|P-value:4.75E-5 ID:SGTC_1874|Compound:staurosporine|FD-Score:8.08|P-value:3.38E-14 ID:SGTC_1877|Compound:wortmannin|FD-Score:9.47|P-value:8.35E-19 ID:SGTC_1880|Compound:MG-132|FD-Score:-3.77|P-value:2.32E-4 ID:SGTC_1907|Compound:st060210|FD-Score:5.05|P-value:1.47E-6 ID:SGTC_2041|Compound:5107442|FD-Score:4.35|P-value:2.77E-5 ID:SGTC_2064|Compound:5220758|FD-Score:4.10|P-value:7.37E-5 ID:SGTC_2068|Compound:5226020|FD-Score:-3.64|P-value:3.58E-4 ID:SGTC_2077|Compound:5212015|FD-Score:4.67|P-value:7.65E-6 ID:SGTC_2132|Compound:5317594|FD-Score:7.35|P-value:4.78E-12 ID:SGTC_2137|Compound:5261020|FD-Score:4.39|P-value:2.37E-5 ID:SGTC_2150|Compound:5556613|FD-Score:4.96|P-value:2.18E-6 ID:SGTC_2233|Compound:sulbentine|FD-Score:5.02|P-value:1.69E-6 ID:SGTC_2239|Compound:6685329|FD-Score:5.36|P-value:3.42E-7 ID:SGTC_2284|Compound:7945075|FD-Score:-4.03|P-value:9.13E-5 ID:SGTC_2290|Compound:7904846|FD-Score:3.41|P-value:7.89E-4 ID:SGTC_2322|Compound:7413319|FD-Score:7.42|P-value:2.96E-12 ID:SGTC_2345|Compound:9052315|FD-Score:4.42|P-value:2.12E-5 ID:SGTC_2430|Compound:5571206|FD-Score:7.03|P-value:3.59E-11 ID:SGTC_2469|Compound:5655019|FD-Score:4.29|P-value:3.58E-5 ID:SGTC_2599|Compound:2',3-dihydroxy-4,4',6'-trimethoxychalcone|FD-Score:4.13|P-value:6.48E-5 ID:SGTC_2618|Compound:avocadyne|FD-Score:3.45|P-value:7.10E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:7.13|P-value:1.94E-11 ID:SGTC_2688|Compound:kanamycin b|FD-Score:4.36|P-value:2.74E-5 ID:SGTC_2806|Compound:7952793|FD-Score:3.82|P-value:2.02E-4 ID:SGTC_2932|Compound:9016711|FD-Score:5.78|P-value:4.27E-8 ID:SGTC_2976|Compound:9009005|FD-Score:5.74|P-value:5.13E-8 ID:SGTC_3059|Compound:1327-0034|FD-Score:5.54|P-value:1.45E-7 ID:SGTC_3060|Compound:1327-0027|FD-Score:3.65|P-value:3.69E-4 ID:SGTC_3063|Compound:1327-0034|FD-Score:6.03|P-value:1.14E-8 ID:SGTC_3093|Compound:9117344|FD-Score:4.22|P-value:4.68E-5 ID:SGTC_3124|Compound:9127567|FD-Score:3.37|P-value:9.03E-4 ID:SGTC_3237|Compound:9133362|FD-Score:4.13|P-value:6.57E-5 ID:SGTC_3303|Compound:9125618|FD-Score:5.47|P-value:2.03E-7

Top fitness defect scores for YHL011C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1877 wortmannin 9.47 25.0 µM 8.35E-19 18 1.42E-21
2 SGTC_1304 1165-0502 8.65 251.0 µM 5.50E-16 16 2.65E-18
3 SGTC_1874 staurosporine 8.08 7.6 µM 3.38E-14 cell wall signaling 6 3.15E-16
4 SGTC_1154 4088-0362 7.86 150.0 µM 1.61E-13 1 1.92E-15
5 SGTC_221 trifluoperazine 7.57 10.3 µM 1.16E-12 NEO1-PIK1 91 1.91E-14
6 SGTC_2322 7413319 7.42 38.2 µM 2.96E-12 ERAD & cell cycle 41 5.64E-14
7 SGTC_974 1326-0144 7.35 138.0 µM 4.74E-12 ERG2 5 9.74E-14
8 SGTC_2132 5317594 7.35 122.7 µM 4.78E-12 SWF1 & branched chain AA biosynthesis 7 9.84E-14
9 SGTC_2651 1327-0036 7.13 5.0 µM 1.94E-11 45 4.98E-13
10 SGTC_337 1315-0038 7.10 354.0 µM 2.36E-11 amide catabolism 3 6.26E-13
11 SGTC_1781 st038725 7.05 66.3 µM 3.25E-11 NEO1 38 9.07E-13
12 SGTC_2430 5571206 7.03 200.0 µM 3.59E-11 15 1.02E-12
13 SGTC_3063 1327-0034 6.03 4.0 µM 1.14E-8 22 8.11E-10
14 SGTC_2932 9016711 5.78 44.5 µM 4.27E-8 13 3.74E-9
15 SGTC_2976 9009005 5.74 19.5 µM 5.13E-8 13 4.62E-9
16 SGTC_3059 1327-0034 5.54 3.0 µM 1.45E-7 29 1.55E-8
17 SGTC_3303 9125618 5.47 40.6 µM 2.03E-7 17 2.27E-8
18 SGTC_2239 6685329 5.36 187.6 µM 3.42E-7 6 4.16E-8
19 SGTC_1562 morin hydrate 5.20 59.1 µM 7.17E-7 2 9.80E-8
20 SGTC_445 e6 berbamine 5.06 66.0 µM 1.37E-6 NEO1-PIK1 60 2.07E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.267 6.12E-56 YPR060C ARO7 hom Chorismate mutase, catalyzes the conversion of chorismate to prephenate to initiate the tyrosine/phenylalanine-specific branch of aromatic amino acid biosynthesis
0.262 7.03E-54 YBL079W NUP170 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC assembly and nucleocytoplasmic transport; both Nup170p and NUP157p are similar to human Nup155p; NUP170 has a paralog, NUP157, that arose from the whole genome duplication
0.253 4.24E-50 YDR392W SPT3 hom Subunit of the SAGA and SAGA-like transcriptional regulatory complexes, interacts with Spt15p to activate transcription of some RNA polymerase II-dependent genes, also functions to inhibit transcription at some promoters
0.244 1.49E-46 YBR068C BAP2 hom High-affinity leucine permease; functions as a branched-chain amino acid permease involved in uptake of leucine, isoleucine and valine; contains 12 predicted transmembrane domains; BAP2 has a paralog, BAP3, that arose from the whole genome duplication
0.240 3.31E-45 YIL052C RPL34B hom Ribosomal 60S subunit protein L34B; homologous to mammalian ribosomal protein L34, no bacterial homolog; RPL34B has a paralog, RPL34A, that arose from the whole genome duplication
0.210 1.82E-34 YDR127W ARO1 hom Pentafunctional arom protein, catalyzes steps 2 through 6 in the biosynthesis of chorismate, which is a precursor to aromatic amino acids
0.208 4.58E-34 YER086W ILV1 hom Threonine deaminase, catalyzes the first step in isoleucine biosynthesis; expression is under general amino acid control; ILV1 locus exhibits highly positioned nucleosomes whose organization is independent of known ILV1 regulation
0.193 1.22E-29 YJR063W RPA12 hom RNA polymerase I subunit A12.2; contains two zinc binding domains, and the N terminal domain is responsible for anchoring to the RNA pol I complex
0.193 1.74E-29 YIR023W DAL81 hom Positive regulator of genes in multiple nitrogen degradation pathways; contains DNA binding domain but does not appear to bind the dodecanucleotide sequence present in the promoter region of many genes involved in allantoin catabolism
0.187 7.30E-28 YNL229C URE2 hom Nitrogen catabolite repression transcriptional regulator that acts by inhibition of GLN3 transcription in good nitrogen source; has glutathione peroxidase activity and can mutate to acquire GST activity; altered form creates [URE3] prion
0.186 1.86E-27 YLR087C CSF1 hom Protein required for fermentation at low temperature; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.184 7.68E-27 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.181 6.03E-26 YCR053W THR4 hom Threonine synthase, conserved protein that catalyzes formation of threonine from O-phosphohomoserine; expression is regulated by the GCN4-mediated general amino acid control pathway
0.176 8.78E-25 YNL032W SIW14 hom Tyrosine phosphatase that plays a role in actin filament organization and endocytosis; localized to the cytoplasm
0.164 1.23E-21 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval