YHL013C / OTU2

Protein of unknown function; may interact with ribosomes, based on co-purification experiments; member of the ovarian tumor-like (OTU) superfamily of predicted cysteine proteases; shows cytoplasmic localization; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL013C / OTU2

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.38|P-value:8.79E-9 ID:SGTC_65|Compound:0798-0905|FD-Score:4.29|P-value:3.38E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:4.45|P-value:1.55E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.26|P-value:4.29E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.71|P-value:7.31E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:-3.05|P-value:9.19E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.74|P-value:6.22E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-3.64|P-value:9.55E-5 ID:SGTC_230|Compound:MPP+|FD-Score:3.45|P-value:1.42E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:3.19|P-value:3.85E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:5.25|P-value:1.91E-8 ID:SGTC_277|Compound:nystatin|FD-Score:3.18|P-value:4.06E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.05|P-value:9.25E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:4.46|P-value:1.48E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.57|P-value:8.83E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.32|P-value:3.38E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:4.55|P-value:9.08E-7 ID:SGTC_619|Compound:1652-1634|FD-Score:3.20|P-value:3.75E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:-4.20|P-value:8.08E-6 ID:SGTC_664|Compound:1222-0054|FD-Score:-3.73|P-value:6.71E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:3.04|P-value:6.80E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.94|P-value:1.76E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:2.98|P-value:8.52E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:4.62|P-value:6.55E-7 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.77|P-value:3.81E-5 ID:SGTC_1342|Compound:1486-1464|FD-Score:-3.74|P-value:6.24E-5 ID:SGTC_1493|Compound:4348-0038|FD-Score:2.95|P-value:9.34E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.05|P-value:6.56E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.14|P-value:6.54E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-3.95|P-value:2.57E-5 ID:SGTC_1856|Compound:st057054|FD-Score:6.59|P-value:2.86E-12 ID:SGTC_1860|Compound:st056228|FD-Score:-3.20|P-value:5.39E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.15|P-value:6.45E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.29|P-value:2.63E-4 ID:SGTC_2378|Compound:9074213|FD-Score:3.57|P-value:8.69E-5 ID:SGTC_2426|Compound:5582046|FD-Score:3.22|P-value:3.52E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.79|P-value:3.47E-5 ID:SGTC_2813|Compound:7971799|FD-Score:-3.08|P-value:8.33E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.49|P-value:1.19E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.41|P-value:1.70E-4 ID:SGTC_2983|Compound:9022671|FD-Score:3.08|P-value:5.78E-4 ID:SGTC_3048|Compound:9093155|FD-Score:3.75|P-value:4.12E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.00|P-value:7.87E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.53|P-value:1.04E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-3.36|P-value:2.92E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.01|P-value:7.62E-4 ID:SGTC_3339|Compound:9145883|FD-Score:3.12|P-value:4.98E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:5.38|P-value:8.79E-9 ID:SGTC_65|Compound:0798-0905|FD-Score:4.29|P-value:3.38E-6 ID:SGTC_138|Compound:0850-0259|FD-Score:4.45|P-value:1.55E-6 ID:SGTC_149|Compound:3970-0822|FD-Score:-3.26|P-value:4.29E-4 ID:SGTC_150|Compound:3970-0969|FD-Score:-3.71|P-value:7.31E-5 ID:SGTC_155|Compound:k072-0202|FD-Score:-3.05|P-value:9.19E-4 ID:SGTC_156|Compound:3474-0145|FD-Score:-3.74|P-value:6.22E-5 ID:SGTC_221|Compound:trifluoperazine|FD-Score:-3.64|P-value:9.55E-5 ID:SGTC_230|Compound:MPP+|FD-Score:3.45|P-value:1.42E-4 ID:SGTC_233|Compound:rapamycin|FD-Score:3.19|P-value:3.85E-4 ID:SGTC_273|Compound:hydroxyurea|FD-Score:5.25|P-value:1.91E-8 ID:SGTC_277|Compound:nystatin|FD-Score:3.18|P-value:4.06E-4 ID:SGTC_373|Compound:0844-0104|FD-Score:-3.05|P-value:9.25E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:4.46|P-value:1.48E-6 ID:SGTC_407|Compound:eburnamonine|FD-Score:3.57|P-value:8.83E-5 ID:SGTC_426|Compound:0988-0037|FD-Score:-3.32|P-value:3.38E-4 ID:SGTC_485|Compound:niflumic acid|FD-Score:4.55|P-value:9.08E-7 ID:SGTC_619|Compound:1652-1634|FD-Score:3.20|P-value:3.75E-4 ID:SGTC_662|Compound:k832-1615|FD-Score:-4.20|P-value:8.08E-6 ID:SGTC_664|Compound:1222-0054|FD-Score:-3.73|P-value:6.71E-5 ID:SGTC_921|Compound:1561-0023|FD-Score:3.04|P-value:6.80E-4 ID:SGTC_976|Compound:1348-1067|FD-Score:3.94|P-value:1.76E-5 ID:SGTC_1022|Compound:4477-0945|FD-Score:2.98|P-value:8.52E-4 ID:SGTC_1166|Compound:k206-0078|FD-Score:4.62|P-value:6.55E-7 ID:SGTC_1273|Compound:0849-0096|FD-Score:3.77|P-value:3.81E-5 ID:SGTC_1342|Compound:1486-1464|FD-Score:-3.74|P-value:6.24E-5 ID:SGTC_1493|Compound:4348-0038|FD-Score:2.95|P-value:9.34E-4 ID:SGTC_1646|Compound:st011709|FD-Score:3.05|P-value:6.56E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-3.14|P-value:6.54E-4 ID:SGTC_1849|Compound:st056239|FD-Score:-3.95|P-value:2.57E-5 ID:SGTC_1856|Compound:st057054|FD-Score:6.59|P-value:2.86E-12 ID:SGTC_1860|Compound:st056228|FD-Score:-3.20|P-value:5.39E-4 ID:SGTC_1901|Compound:st060216|FD-Score:-3.15|P-value:6.45E-4 ID:SGTC_1931|Compound:st074438|FD-Score:3.29|P-value:2.63E-4 ID:SGTC_2378|Compound:9074213|FD-Score:3.57|P-value:8.69E-5 ID:SGTC_2426|Compound:5582046|FD-Score:3.22|P-value:3.52E-4 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.79|P-value:3.47E-5 ID:SGTC_2813|Compound:7971799|FD-Score:-3.08|P-value:8.33E-4 ID:SGTC_2821|Compound:7996764|FD-Score:3.49|P-value:1.19E-4 ID:SGTC_2930|Compound:9013230|FD-Score:3.41|P-value:1.70E-4 ID:SGTC_2983|Compound:9022671|FD-Score:3.08|P-value:5.78E-4 ID:SGTC_3048|Compound:9093155|FD-Score:3.75|P-value:4.12E-5 ID:SGTC_3063|Compound:1327-0034|FD-Score:3.00|P-value:7.87E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.53|P-value:1.04E-4 ID:SGTC_3233|Compound:9132340|FD-Score:-3.36|P-value:2.92E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.01|P-value:7.62E-4 ID:SGTC_3339|Compound:9145883|FD-Score:3.12|P-value:4.98E-4

Top fitness defect scores for YHL013C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1856 st057054 6.59 55.7 µM 2.86E-12 5 2.22E-11
2 SGTC_58 0957-0393 5.38 9.0 µM 8.79E-9 cell wall 249 3.68E-8
3 SGTC_273 hydroxyurea 5.25 18.1 mM 1.91E-8 heme biosynthesis & mitochondrial translocase 14 7.55E-8
4 SGTC_1166 k206-0078 4.62 225.0 µM 6.55E-7 8 1.96E-6
5 SGTC_485 niflumic acid 4.55 177.0 µM 9.08E-7 5 2.65E-6
6 SGTC_392 N-ethylmaleimide 4.46 8.3 µM 1.48E-6 RNA pol III & RNase P/MRP 8 4.16E-6
7 SGTC_138 0850-0259 4.45 40.9 µM 1.55E-6 cell wall 169 4.35E-6
8 SGTC_65 0798-0905 4.29 35.0 µM 3.38E-6 calcium & mitochondrial duress 73 8.92E-6
9 SGTC_976 1348-1067 3.94 47.6 µM 1.76E-5 60S ribosome export 32 4.07E-5
10 SGTC_2489 cantharidin 3.79 73.4 µM 3.47E-5 cell wall signaling 49 7.64E-5
11 SGTC_1273 0849-0096 3.77 14.1 µM 3.81E-5 RPP1 & pyrimidine depletion 27 8.32E-5
12 SGTC_3048 9093155 3.75 49.5 µM 4.12E-5 7 8.94E-5
13 SGTC_2378 9074213 3.57 121.4 µM 8.69E-5 RSC complex & mRNA processing 30 1.78E-4
14 SGTC_407 eburnamonine 3.57 100.0 µM 8.83E-5 22 1.80E-4
15 SGTC_3213 9129884 3.53 49.5 µM 1.04E-4 30 2.09E-4
16 SGTC_2821 7996764 3.49 71.4 µM 1.19E-4 58 2.38E-4
17 SGTC_230 MPP+ 3.45 141.7 µM 1.42E-4 30 2.80E-4
18 SGTC_2930 9013230 3.41 14.9 µM 1.70E-4 heme biosynthesis & mitochondrial translocase 61 3.30E-4
19 SGTC_1931 st074438 3.29 57.1 µM 2.63E-4 39 4.94E-4
20 SGTC_2426 5582046 3.22 32.2 µM 3.52E-4 57 6.45E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.203 1.69E-32 YBR156C SLI15 hom Subunit of the conserved chromosomal passenger complex (CPC; Ipl1p-Sli15p-Bir1p-Nbl1p), which regulates kinetochore-microtubule attachments, activation of the spindle tension checkpoint, and mitotic spindle disassembly
0.200 1.24E-31 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together
0.198 4.95E-31 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.186 1.85E-27 YLR150W STM1 hom Protein required for optimal translation under nutrient stress; perturbs association of Yef3p with ribosomes; involved in TOR signaling; binds G4 quadruplex and purine motif triplex nucleic acid; helps maintain telomere structure; protein abundance increases in response to DNA replication stress; serves as a ribosome preservation factor both during quiescence and recovery
0.172 1.08E-23 YAL015C NTG1 hom DNA N-glycosylase and apurinic/apyrimidinic (AP) lyase; involved in base excision repair; acts in both nucleus and mitochondrion; creates a double-strand break at mtDNA origins that stimulates replication in response to oxidative stress; NTG1 has a paralog, NTG2, that arose from the whole genome duplication
0.171 2.37E-23 YJL027C_p YJL027C_p hom Putative protein of unknown function
0.169 5.39E-23 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.155 1.43E-19 YKL033W-A_p YKL033W-A_p hom Putative protein of unknown function; similar to uncharacterized proteins from other fungi
0.155 1.86E-19 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.153 4.33E-19 YNL289W PCL1 hom Cyclin, interacts with cyclin-dependent kinase Pho85p; member of the Pcl1,2-like subfamily, involved in the regulation of polarized growth and morphogenesis and progression through the cell cycle; is ubiquitinated by Dma1p; phosphorylation by Pho85p targets it for degradation; localizes to sites of polarized cell growth
0.153 4.95E-19 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.150 2.45E-18 YDR335W MSN5 hom Karyopherin involved in nuclear import and export of proteins, including import of replication protein A and export of Swi6p, Far1p, and Pho4p; required for re-export of mature tRNAs after their retrograde import from the cytoplasm
0.150 2.53E-18 YKR042W UTH1 hom Protein involved in cell wall biogenesis; required for mitochondrial autophagy; mitochondrial outer membrane and cell wall localized SUN family member; involved in the oxidative stress response, life span during starvation, and cell death; UTH1 has a paralog, NCA3, that arose from the whole genome duplication
0.149 4.15E-18 YPL269W KAR9 hom Karyogamy protein required for correct positioning of the mitotic spindle and for orienting cytoplasmic microtubules, localizes at the shmoo tip in mating cells and at the tip of the growing bud in small-budded cells through anaphase
0.148 5.72E-18 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication