YHL022C / SPO11

Meiosis-specific protein that initiates meiotic recombination by catalyzing the formation of double-strand breaks in DNA via a transesterification reaction; required for homologous chromosome pairing and synaptonemal complex formation

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL022C / SPO11

Click on Significant Values for Screen Details ID:SGTC_38|Compound:0929-0051|FD-Score:3.35|P-value:6.34E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:3.12|P-value:1.76E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.40|P-value:5.02E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:3.04|P-value:2.44E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:-4.69|P-value:8.92E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:2.93|P-value:3.88E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:-4.77|P-value:5.41E-8 ID:SGTC_385|Compound:1494-0393|FD-Score:-4.31|P-value:8.30E-7 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.25|P-value:1.63E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:5.04|P-value:4.99E-9 ID:SGTC_556|Compound:4058-0063|FD-Score:3.90|P-value:4.33E-6 ID:SGTC_580|Compound:1123-0028|FD-Score:2.79|P-value:6.92E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:-2.88|P-value:7.44E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:-3.54|P-value:4.38E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.07|P-value:2.19E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.77|P-value:8.25E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:2.81|P-value:6.33E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:2.74|P-value:8.33E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.61|P-value:1.84E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:-2.89|P-value:6.98E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.29|P-value:8.31E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.29|P-value:8.36E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:3.13|P-value:1.67E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.08|P-value:2.09E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.12|P-value:1.78E-4 ID:SGTC_1651|Compound:st011928|FD-Score:-3.55|P-value:4.17E-5 ID:SGTC_1652|Compound:st011932|FD-Score:3.01|P-value:2.82E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.48|P-value:3.54E-5 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:-3.26|P-value:1.54E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.58|P-value:3.66E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.26|P-value:1.55E-4 ID:SGTC_1736|Compound:st036774|FD-Score:5.25|P-value:1.17E-9 ID:SGTC_1843|Compound:st055809|FD-Score:3.52|P-value:2.88E-5 ID:SGTC_1866|Compound:st057638|FD-Score:2.88|P-value:4.90E-4 ID:SGTC_2011|Compound:4012611|FD-Score:-2.89|P-value:7.05E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.00|P-value:2.98E-4 ID:SGTC_2043|Compound:5101342|FD-Score:-3.84|P-value:9.89E-6 ID:SGTC_2160|Compound:5660466|FD-Score:2.95|P-value:3.60E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:2.94|P-value:3.80E-4 ID:SGTC_2181|Compound:6316240|FD-Score:-3.43|P-value:7.08E-5 ID:SGTC_2207|Compound:7258803|FD-Score:2.97|P-value:3.39E-4 ID:SGTC_2279|Compound:7948423|FD-Score:-2.89|P-value:7.09E-4 ID:SGTC_2295|Compound:7971645|FD-Score:3.70|P-value:1.19E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:6.53|P-value:6.15E-14 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.63|P-value:2.91E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.61|P-value:1.88E-5 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:4.39|P-value:2.87E-7 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.13|P-value:1.70E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.45|P-value:3.94E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:2.70|P-value:9.56E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.35|P-value:6.40E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.74|P-value:9.59E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.05|P-value:1.94E-6 ID:SGTC_2591|Compound:skatole|FD-Score:-4.56|P-value:2.00E-7 ID:SGTC_2718|Compound:lomofungin|FD-Score:-3.93|P-value:6.56E-6 ID:SGTC_2793|Compound:7325131|FD-Score:3.50|P-value:3.19E-5 ID:SGTC_2818|Compound:7996756|FD-Score:2.71|P-value:9.50E-4 ID:SGTC_2820|Compound:7989630|FD-Score:2.77|P-value:7.42E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.55|P-value:2.44E-5 ID:SGTC_2853|Compound:9015042|FD-Score:-4.74|P-value:6.63E-8 ID:SGTC_2858|Compound:9025781|FD-Score:3.38|P-value:5.58E-5 ID:SGTC_2900|Compound:5328528|FD-Score:3.03|P-value:2.64E-4 ID:SGTC_2908|Compound:7930301|FD-Score:4.18|P-value:9.29E-7 ID:SGTC_2994|Compound:9053520|FD-Score:6.32|P-value:3.54E-13 ID:SGTC_2997|Compound:9067075|FD-Score:3.54|P-value:2.68E-5 ID:SGTC_2999|Compound:9069765|FD-Score:3.88|P-value:4.85E-6 ID:SGTC_3118|Compound:9124250|FD-Score:4.19|P-value:9.06E-7 ID:SGTC_3131|Compound:9127911|FD-Score:2.82|P-value:6.14E-4 ID:SGTC_38|Compound:0929-0051|FD-Score:3.35|P-value:6.34E-5 ID:SGTC_39|Compound:0986-0283|FD-Score:3.12|P-value:1.76E-4 ID:SGTC_67|Compound:0335-0847|FD-Score:3.40|P-value:5.02E-5 ID:SGTC_72|Compound:0744-0168|FD-Score:3.04|P-value:2.44E-4 ID:SGTC_171|Compound:k064-0041|FD-Score:-4.69|P-value:8.92E-8 ID:SGTC_234|Compound:nsc-17383|FD-Score:2.93|P-value:3.88E-4 ID:SGTC_384|Compound:k007-0144|FD-Score:-4.77|P-value:5.41E-8 ID:SGTC_385|Compound:1494-0393|FD-Score:-4.31|P-value:8.30E-7 ID:SGTC_504|Compound:lovastatin|FD-Score:-3.25|P-value:1.63E-4 ID:SGTC_551|Compound:k048-0028|FD-Score:5.04|P-value:4.99E-9 ID:SGTC_556|Compound:4058-0063|FD-Score:3.90|P-value:4.33E-6 ID:SGTC_580|Compound:1123-0028|FD-Score:2.79|P-value:6.92E-4 ID:SGTC_688|Compound:0527-0193|FD-Score:-2.88|P-value:7.44E-4 ID:SGTC_736|Compound:0958-0254|FD-Score:-3.54|P-value:4.38E-5 ID:SGTC_790|Compound:1575-0012|FD-Score:3.07|P-value:2.19E-4 ID:SGTC_793|Compound:0488-0545|FD-Score:3.77|P-value:8.25E-6 ID:SGTC_800|Compound:3448-7362|FD-Score:2.81|P-value:6.33E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:2.74|P-value:8.33E-4 ID:SGTC_1001|Compound:4048-4355|FD-Score:3.61|P-value:1.84E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:-2.89|P-value:6.98E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.29|P-value:8.31E-5 ID:SGTC_1254|Compound:0550-0035|FD-Score:3.29|P-value:8.36E-5 ID:SGTC_1460|Compound:k062-0059|FD-Score:3.13|P-value:1.67E-4 ID:SGTC_1483|Compound:3453-0561|FD-Score:3.08|P-value:2.09E-4 ID:SGTC_1551|Compound:6-bromo-3'-methylflavone|FD-Score:3.12|P-value:1.78E-4 ID:SGTC_1651|Compound:st011928|FD-Score:-3.55|P-value:4.17E-5 ID:SGTC_1652|Compound:st011932|FD-Score:3.01|P-value:2.82E-4 ID:SGTC_1677|Compound:st019204|FD-Score:3.48|P-value:3.54E-5 ID:SGTC_1692|Compound:fluoroquinolonic acid|FD-Score:-3.26|P-value:1.54E-4 ID:SGTC_1729|Compound:st035615|FD-Score:-3.58|P-value:3.66E-5 ID:SGTC_1732|Compound:st034308|FD-Score:-3.26|P-value:1.55E-4 ID:SGTC_1736|Compound:st036774|FD-Score:5.25|P-value:1.17E-9 ID:SGTC_1843|Compound:st055809|FD-Score:3.52|P-value:2.88E-5 ID:SGTC_1866|Compound:st057638|FD-Score:2.88|P-value:4.90E-4 ID:SGTC_2011|Compound:4012611|FD-Score:-2.89|P-value:7.05E-4 ID:SGTC_2034|Compound:5197015|FD-Score:3.00|P-value:2.98E-4 ID:SGTC_2043|Compound:5101342|FD-Score:-3.84|P-value:9.89E-6 ID:SGTC_2160|Compound:5660466|FD-Score:2.95|P-value:3.60E-4 ID:SGTC_2169|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:2.94|P-value:3.80E-4 ID:SGTC_2181|Compound:6316240|FD-Score:-3.43|P-value:7.08E-5 ID:SGTC_2207|Compound:7258803|FD-Score:2.97|P-value:3.39E-4 ID:SGTC_2279|Compound:7948423|FD-Score:-2.89|P-value:7.09E-4 ID:SGTC_2295|Compound:7971645|FD-Score:3.70|P-value:1.19E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:6.53|P-value:6.15E-14 ID:SGTC_2419|Compound:paclitaxel|FD-Score:-3.63|P-value:2.91E-5 ID:SGTC_2433|Compound:5607874|FD-Score:3.61|P-value:1.88E-5 ID:SGTC_2497|Compound:7-hydroxyflavone|FD-Score:4.39|P-value:2.87E-7 ID:SGTC_2499|Compound:avocadenofuran|FD-Score:3.13|P-value:1.70E-4 ID:SGTC_2536|Compound:dehydroabietamide|FD-Score:3.45|P-value:3.94E-5 ID:SGTC_2563|Compound:tomatine|FD-Score:2.70|P-value:9.56E-4 ID:SGTC_2566|Compound:crystal violet carbinol|FD-Score:3.35|P-value:6.40E-5 ID:SGTC_2581|Compound:avocatin a|FD-Score:3.74|P-value:9.59E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:4.05|P-value:1.94E-6 ID:SGTC_2591|Compound:skatole|FD-Score:-4.56|P-value:2.00E-7 ID:SGTC_2718|Compound:lomofungin|FD-Score:-3.93|P-value:6.56E-6 ID:SGTC_2793|Compound:7325131|FD-Score:3.50|P-value:3.19E-5 ID:SGTC_2818|Compound:7996756|FD-Score:2.71|P-value:9.50E-4 ID:SGTC_2820|Compound:7989630|FD-Score:2.77|P-value:7.42E-4 ID:SGTC_2825|Compound:7998094|FD-Score:3.55|P-value:2.44E-5 ID:SGTC_2853|Compound:9015042|FD-Score:-4.74|P-value:6.63E-8 ID:SGTC_2858|Compound:9025781|FD-Score:3.38|P-value:5.58E-5 ID:SGTC_2900|Compound:5328528|FD-Score:3.03|P-value:2.64E-4 ID:SGTC_2908|Compound:7930301|FD-Score:4.18|P-value:9.29E-7 ID:SGTC_2994|Compound:9053520|FD-Score:6.32|P-value:3.54E-13 ID:SGTC_2997|Compound:9067075|FD-Score:3.54|P-value:2.68E-5 ID:SGTC_2999|Compound:9069765|FD-Score:3.88|P-value:4.85E-6 ID:SGTC_3118|Compound:9124250|FD-Score:4.19|P-value:9.06E-7 ID:SGTC_3131|Compound:9127911|FD-Score:2.82|P-value:6.14E-4

Top fitness defect scores for YHL022C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2406 ethylparaben 6.53 405.7 µM 6.15E-14 7 3.23E-11
2 SGTC_2994 9053520 6.32 71.4 µM 3.54E-13 RPP1 & pyrimidine depletion 4 1.28E-10
3 SGTC_1736 st036774 5.25 51.0 µM 1.17E-9 12 7.51E-8
4 SGTC_551 k048-0028 5.04 34.9 µM 4.99E-9 3 2.35E-7
5 SGTC_2497 7-hydroxyflavone 4.39 100.0 µM 2.87E-7 14 5.70E-6
6 SGTC_3118 9124250 4.19 49.5 µM 9.06E-7 10 1.41E-5
7 SGTC_2908 7930301 4.18 100.0 µM 9.29E-7 19 1.44E-5
8 SGTC_2582 agaric acid 4.05 100.0 µM 1.94E-6 redox potentiating 304 2.57E-5
9 SGTC_556 4058-0063 3.90 51.5 µM 4.33E-6 27 4.84E-5
10 SGTC_2999 9069765 3.88 71.4 µM 4.85E-6 amide catabolism 29 5.30E-5
11 SGTC_793 0488-0545 3.77 106.0 µM 8.25E-6 18 8.05E-5
12 SGTC_2581 avocatin a 3.74 64.0 µM 9.59E-6 sphingolipid biosynthesis & PDR1 60 9.06E-5
13 SGTC_2295 7971645 3.70 200.0 µM 1.19E-5 28 1.08E-4
14 SGTC_1001 4048-4355 3.61 44.4 µM 1.84E-5 copper-dependent oxidative stress 45 1.51E-4
15 SGTC_2433 5607874 3.61 27.8 µM 1.88E-5 25 1.54E-4
16 SGTC_2825 7998094 3.55 58.4 µM 2.44E-5 fatty acid desaturase (OLE1) 44 1.89E-4
17 SGTC_2997 9067075 3.54 71.4 µM 2.68E-5 unfolded protein response 71 2.04E-4
18 SGTC_1843 st055809 3.52 45.5 µM 2.88E-5 25 2.15E-4
19 SGTC_2793 7325131 3.50 81.8 µM 3.19E-5 25 2.34E-4
20 SGTC_1677 st019204 3.48 53.9 µM 3.54E-5 32 2.54E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.117 1.18E-11 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.115 2.57E-11 YNL105W_d RRT16_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps verified gene INP52; identified in a screen for mutants with decreased levels of rDNA transcription
0.111 1.14E-10 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.107 5.73E-10 YLR206W ENT2 hom Epsin-like protein required for endocytosis and actin patch assembly and functionally redundant with Ent1p; contains clathrin-binding motif at C-terminus
0.103 2.42E-9 YML052W SUR7 hom Plasma membrane protein that localizes to furrow-like invaginations (MCC patches); component of eisosomes; associated with endocytosis, along with Pil1p and Lsp1p; sporulation and plasma membrane sphingolipid content are altered in mutants
0.099 1.07E-8 YIL018W RPL2B hom Ribosomal 60S subunit protein L2B; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2B has a paralog, RPL2A, that arose from the whole genome duplication; expression is upregulated at low temperatures
0.098 1.21E-8 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.097 2.06E-8 YJR033C RAV1 hom Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate
0.094 4.30E-8 YBL064C PRX1 hom Mitochondrial peroxiredoxin with thioredoxin peroxidase activity; has a role in reduction of hydroperoxides; reactivation requires Trr2p and glutathione; induced during respiratory growth and oxidative stress; phosphorylated; protein abundance increases in response to DNA replication stress
0.093 6.27E-8 YKL001C MET14 hom Adenylylsulfate kinase, required for sulfate assimilation and involved in methionine metabolism
0.093 6.48E-8 YLL042C ATG10 hom Conserved E2-like conjugating enzyme that mediates formation of the Atg12p-Atg5p conjugate, which is a critical step in autophagy
0.092 8.25E-8 YOR306C MCH5 hom Plasma membrane riboflavin transporter; facilitates the uptake of vitamin B2; required for FAD-dependent processes; sequence similarity to mammalian monocarboxylate permeases, however mutants are not deficient in monocarboxylate transport
0.091 1.22E-7 YDL194W SNF3 hom Plasma membrane low glucose sensor, regulates glucose transport; contains 12 predicted transmembrane segments and a long C-terminal tail required for induction of hexose transporters; also senses fructose and mannose; SNF3 has a paralog, RGT2, that arose from the whole genome duplication
0.091 1.44E-7 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.088 3.84E-7 YJR041C URB2 het Nucleolar protein required for normal metabolism of the rRNA primary transcript, proposed to be involved in ribosome biogenesis