YHL023C / NPR3

Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; required for Npr2p phosphorylation and Iml1p-Npr2p interaction; null mutant shows delayed meiotic DNA replication and double-strand break repair

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL023C / NPR3

Click on Significant Values for Screen Details ID:SGTC_36|Compound:0335-0881|FD-Score:3.83|P-value:9.57E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.92|P-value:6.85E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.01|P-value:3.21E-7 ID:SGTC_114|Compound:0364-0001|FD-Score:3.80|P-value:1.06E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.46|P-value:3.78E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:6.32|P-value:3.10E-10 ID:SGTC_142|Compound:0335-0849|FD-Score:6.22|P-value:5.99E-10 ID:SGTC_158|Compound:k038-0087|FD-Score:3.50|P-value:3.28E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.82|P-value:1.01E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.64|P-value:1.76E-8 ID:SGTC_236|Compound:1509-0011|FD-Score:3.46|P-value:3.82E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.06|P-value:2.62E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:3.60|P-value:2.31E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:3.37|P-value:5.18E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:4.12|P-value:2.95E-5 ID:SGTC_393|Compound:acivicin|FD-Score:9.23|P-value:7.36E-20 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.05|P-value:2.72E-5 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.61|P-value:2.05E-8 ID:SGTC_541|Compound:4513-1321|FD-Score:3.28|P-value:7.08E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:-3.28|P-value:5.32E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.31|P-value:6.41E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.32|P-value:6.34E-4 ID:SGTC_847|Compound:0342-0274|FD-Score:3.34|P-value:5.85E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:5.20|P-value:1.87E-7 ID:SGTC_1011|Compound:4112-3381|FD-Score:6.55|P-value:7.51E-11 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.28|P-value:1.26E-7 ID:SGTC_1018|Compound:4259-0013|FD-Score:4.13|P-value:2.86E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.59|P-value:2.42E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.21|P-value:9.22E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.83|P-value:9.53E-5 ID:SGTC_1208|Compound:2323-2347|FD-Score:-3.36|P-value:3.96E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.33|P-value:6.11E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.75|P-value:1.29E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.91|P-value:7.06E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.11|P-value:2.19E-5 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.75|P-value:1.79E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:-5.08|P-value:2.23E-7 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.28|P-value:7.18E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.35|P-value:5.66E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:-5.03|P-value:2.85E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.31|P-value:4.74E-4 ID:SGTC_1629|Compound:st005085|FD-Score:5.81|P-value:6.90E-9 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.70|P-value:1.59E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.49|P-value:3.41E-4 ID:SGTC_1687|Compound:st020778|FD-Score:5.46|P-value:4.79E-8 ID:SGTC_1694|Compound:st024011|FD-Score:4.55|P-value:4.55E-6 ID:SGTC_1712|Compound:st032288|FD-Score:-3.48|P-value:2.64E-4 ID:SGTC_1802|Compound:st050112|FD-Score:3.39|P-value:4.86E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.41|P-value:4.65E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.39|P-value:4.90E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.27|P-value:7.39E-4 ID:SGTC_1975|Compound:st070967|FD-Score:-3.27|P-value:5.53E-4 ID:SGTC_1984|Compound:st072354|FD-Score:3.25|P-value:8.02E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.19|P-value:9.79E-4 ID:SGTC_2055|Compound:5235184|FD-Score:-3.87|P-value:5.91E-5 ID:SGTC_2508|Compound:celastrol|FD-Score:4.05|P-value:4.00E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:-3.70|P-value:1.12E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-5.21|P-value:1.12E-7 ID:SGTC_2645|Compound:abamectin|FD-Score:4.40|P-value:8.94E-6 ID:SGTC_2785|Compound:7756463|FD-Score:4.28|P-value:1.49E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:5.32|P-value:1.03E-7 ID:SGTC_3162|Compound:9099653|FD-Score:-3.92|P-value:4.81E-5 ID:SGTC_3237|Compound:9133362|FD-Score:4.63|P-value:3.19E-6 ID:SGTC_3355|Compound:9151058|FD-Score:3.42|P-value:4.34E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.83|P-value:9.57E-5 ID:SGTC_43|Compound:0570-0105|FD-Score:3.92|P-value:6.85E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:-5.01|P-value:3.21E-7 ID:SGTC_114|Compound:0364-0001|FD-Score:3.80|P-value:1.06E-4 ID:SGTC_124|Compound:0136-0217|FD-Score:3.46|P-value:3.78E-4 ID:SGTC_137|Compound:0438-0332|FD-Score:6.32|P-value:3.10E-10 ID:SGTC_142|Compound:0335-0849|FD-Score:6.22|P-value:5.99E-10 ID:SGTC_158|Compound:k038-0087|FD-Score:3.50|P-value:3.28E-4 ID:SGTC_159|Compound:3970-0959|FD-Score:3.82|P-value:1.01E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.64|P-value:1.76E-8 ID:SGTC_236|Compound:1509-0011|FD-Score:3.46|P-value:3.82E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:-4.06|P-value:2.62E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:3.60|P-value:2.31E-4 ID:SGTC_349|Compound:k015-0014|FD-Score:3.37|P-value:5.18E-4 ID:SGTC_377|Compound:3970-0964|FD-Score:4.12|P-value:2.95E-5 ID:SGTC_393|Compound:acivicin|FD-Score:9.23|P-value:7.36E-20 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.05|P-value:2.72E-5 ID:SGTC_488|Compound:nitrendipine|FD-Score:5.61|P-value:2.05E-8 ID:SGTC_541|Compound:4513-1321|FD-Score:3.28|P-value:7.08E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:-3.28|P-value:5.32E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.31|P-value:6.41E-4 ID:SGTC_653|Compound:0203-0016|FD-Score:3.32|P-value:6.34E-4 ID:SGTC_847|Compound:0342-0274|FD-Score:3.34|P-value:5.85E-4 ID:SGTC_922|Compound:1866-0035|FD-Score:5.20|P-value:1.87E-7 ID:SGTC_1011|Compound:4112-3381|FD-Score:6.55|P-value:7.51E-11 ID:SGTC_1016|Compound:4226-1401|FD-Score:5.28|P-value:1.26E-7 ID:SGTC_1018|Compound:4259-0013|FD-Score:4.13|P-value:2.86E-5 ID:SGTC_1025|Compound:k007-0731|FD-Score:3.59|P-value:2.42E-4 ID:SGTC_1028|Compound:k015-0029|FD-Score:3.21|P-value:9.22E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.83|P-value:9.53E-5 ID:SGTC_1208|Compound:2323-2347|FD-Score:-3.36|P-value:3.96E-4 ID:SGTC_1334|Compound:1486-0610|FD-Score:3.33|P-value:6.11E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:3.75|P-value:1.29E-4 ID:SGTC_1342|Compound:1486-1464|FD-Score:3.91|P-value:7.06E-5 ID:SGTC_1347|Compound:1493-0251|FD-Score:-4.11|P-value:2.19E-5 ID:SGTC_1448|Compound:4255-0083|FD-Score:4.75|P-value:1.79E-6 ID:SGTC_1450|Compound:4112-4260|FD-Score:-5.08|P-value:2.23E-7 ID:SGTC_1452|Compound:4194-0072|FD-Score:3.28|P-value:7.18E-4 ID:SGTC_1455|Compound:4262-0120|FD-Score:3.35|P-value:5.66E-4 ID:SGTC_1464|Compound:k208-0027|FD-Score:-5.03|P-value:2.85E-7 ID:SGTC_1597|Compound:7-benzyloxygramine|FD-Score:-3.31|P-value:4.74E-4 ID:SGTC_1629|Compound:st005085|FD-Score:5.81|P-value:6.90E-9 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.70|P-value:1.59E-4 ID:SGTC_1633|Compound:st005664|FD-Score:3.49|P-value:3.41E-4 ID:SGTC_1687|Compound:st020778|FD-Score:5.46|P-value:4.79E-8 ID:SGTC_1694|Compound:st024011|FD-Score:4.55|P-value:4.55E-6 ID:SGTC_1712|Compound:st032288|FD-Score:-3.48|P-value:2.64E-4 ID:SGTC_1802|Compound:st050112|FD-Score:3.39|P-value:4.86E-4 ID:SGTC_1806|Compound:st050005|FD-Score:3.41|P-value:4.65E-4 ID:SGTC_1877|Compound:wortmannin|FD-Score:3.39|P-value:4.90E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.27|P-value:7.39E-4 ID:SGTC_1975|Compound:st070967|FD-Score:-3.27|P-value:5.53E-4 ID:SGTC_1984|Compound:st072354|FD-Score:3.25|P-value:8.02E-4 ID:SGTC_2005|Compound:4024620|FD-Score:3.19|P-value:9.79E-4 ID:SGTC_2055|Compound:5235184|FD-Score:-3.87|P-value:5.91E-5 ID:SGTC_2508|Compound:celastrol|FD-Score:4.05|P-value:4.00E-5 ID:SGTC_2620|Compound:irigenin|FD-Score:-3.70|P-value:1.12E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-5.21|P-value:1.12E-7 ID:SGTC_2645|Compound:abamectin|FD-Score:4.40|P-value:8.94E-6 ID:SGTC_2785|Compound:7756463|FD-Score:4.28|P-value:1.49E-5 ID:SGTC_3059|Compound:1327-0034|FD-Score:5.32|P-value:1.03E-7 ID:SGTC_3162|Compound:9099653|FD-Score:-3.92|P-value:4.81E-5 ID:SGTC_3237|Compound:9133362|FD-Score:4.63|P-value:3.19E-6 ID:SGTC_3355|Compound:9151058|FD-Score:3.42|P-value:4.34E-4

Top fitness defect scores for YHL023C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_393 acivicin 9.23 6.2 µM 7.36E-20 2 1.35E-20
2 SGTC_1011 4112-3381 6.55 17.4 µM 7.51E-11 11 2.92E-11
3 SGTC_137 0438-0332 6.32 85.4 µM 3.10E-10 ubiquinone biosynthesis & proteasome 57 1.27E-10
4 SGTC_142 0335-0849 6.22 62.7 µM 5.99E-10 ubiquinone biosynthesis & proteasome 57 2.52E-10
5 SGTC_1629 st005085 5.81 44.9 µM 6.90E-9 RPP1 & pyrimidine depletion 8 3.18E-9
6 SGTC_168 3970-0862 5.64 530.5 µM 1.76E-8 53 8.41E-9
7 SGTC_488 nitrendipine 5.61 139.0 µM 2.05E-8 17 9.84E-9
8 SGTC_1687 st020778 5.46 55.4 µM 4.79E-8 122 2.38E-8
9 SGTC_3059 1327-0034 5.32 3.0 µM 1.03E-7 31 5.26E-8
10 SGTC_1016 4226-1401 5.28 38.0 µM 1.26E-7 RNA pol III & RNase P/MRP 6 6.46E-8
11 SGTC_922 1866-0035 5.20 480.0 µM 1.87E-7 excess fatty acid 28 9.77E-8
12 SGTC_1448 4255-0083 4.75 152.0 µM 1.79E-6 ERG2 8 1.02E-6
13 SGTC_3237 9133362 4.63 49.5 µM 3.19E-6 21 1.86E-6
14 SGTC_1694 st024011 4.55 44.9 µM 4.55E-6 ERG2 23 2.69E-6
15 SGTC_2645 abamectin 4.40 100.0 µM 8.94E-6 heme requiring 22 5.42E-6
16 SGTC_2785 7756463 4.28 72.7 µM 1.49E-5 21 9.22E-6
17 SGTC_1018 4259-0013 4.13 28.1 µM 2.86E-5 11 1.82E-5
18 SGTC_377 3970-0964 4.12 33.1 µM 2.95E-5 35 1.88E-5
19 SGTC_2508 celastrol 4.05 40.5 µM 4.00E-5 PDR1 17 2.57E-5
20 SGTC_43 0570-0105 3.92 72.5 µM 6.85E-5 ERG2 95 4.51E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.244 9.23E-47 YEL062W NPR2 hom Subunit of SEA (Seh1-associated), Npr2/3, and Iml1p complexes; Npr2/3 complex mediates downregulation of TORC1 activity upon amino acid limitation; SEA complex is a coatomer-related complex that associates dynamically with the vacuole; Iml1p complex (Iml1p-Npr2p-Npr3p) is required for non-nitrogen-starvation (NNS)-induced autophagy; Iml1p interacts primarily with phosphorylated Npr2p; homolog of human NPRL2; target of Grr1p; required for growth on urea and proline
0.208 3.84E-34 YDL183C YDL183C hom Mitochondrial inner-membrane protein thought to be involved in the formation of an active mitochondrial K+/H+ exchanger (KHE) system; non-essential gene
0.191 7.67E-29 YMR015C ERG5 hom C-22 sterol desaturase, a cytochrome P450 enzyme that catalyzes the formation of the C-22(23) double bond in the sterol side chain in ergosterol biosynthesis; may be a target of azole antifungal drugs
0.184 4.34E-27 YDR204W COQ4 hom Protein with a role in ubiquinone (Coenzyme Q) biosynthesis, possibly functioning in stabilization of Coq7p; located on the matrix face of the mitochondrial inner membrane; component of a mitochondrial ubiquinone-synthesizing complex
0.172 8.82E-24 YOL096C COQ3 hom O-methyltransferase, catalyzes two different O-methylation steps in ubiquinone (Coenzyme Q) biosynthesis; component of a mitochondrial ubiquinone-synthesizing complex; phosphoprotein
0.169 5.46E-23 YLR201C COQ9 hom Protein required for ubiquinone (coenzyme Q) biosynthesis and respiratory growth; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes
0.168 1.18E-22 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.165 7.72E-22 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.165 8.08E-22 YML034C-A_d YML034C-A_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.164 1.27E-21 YPR064W_d YPR064W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.162 4.40E-21 YJL072C PSF2 het Subunit of the GINS complex (Sld5p, Psf1p, Psf2p, Psf3p), which is localized to DNA replication origins and implicated in assembly of the DNA replication machinery
0.159 1.65E-20 YOR125C CAT5 hom Protein required for ubiquinone (Coenzyme Q) biosynthesis; localizes to the matrix face of the mitochondrial inner membrane in a large complex with ubiquinone biosynthetic enzymes; required for gluconeogenic gene activation
0.158 3.51E-20 YNR041C COQ2 hom Para hydroxybenzoate: polyprenyl transferase, catalyzes the second step in ubiquinone (coenzyme Q) biosynthesis
0.157 5.02E-20 YML034W SRC1 hom Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
0.157 7.08E-20 YJR103W URA8 hom Minor CTP synthase isozyme (see also URA7); catalyzes the ATP-dependent transfer of the amide nitrogen from glutamine to UTP, forming CTP, the final step in de novo biosynthesis of pyrimidines; involved in phospholipid biosynthesis; capable of forming cytoplasmic filaments termed cytoophidium, especially during conditions of glucose depletion; URA8 has a paralog, URA7, that arose from the whole genome duplication