YHL030W / ECM29

Scaffold protein; assists in association of the proteasome core particle with the regulatory particle; degraded by the mature proteasome after assembly; contains HEAT-like repeats; protein increases in abundance and relocalizes from nucleus to cytoplasm upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL030W / ECM29

Click on Significant Values for Screen Details ID:SGTC_65|Compound:0798-0905|FD-Score:3.13|P-value:5.66E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.03|P-value:8.38E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.53|P-value:1.23E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.05|P-value:7.46E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:3.86|P-value:2.94E-5 ID:SGTC_277|Compound:nystatin|FD-Score:-3.02|P-value:8.72E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.39|P-value:2.07E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.37|P-value:2.23E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.35|P-value:2.47E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.61|P-value:9.14E-5 ID:SGTC_836|Compound:0330-0104|FD-Score:-3.32|P-value:2.87E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:-3.10|P-value:6.51E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:5.02|P-value:8.55E-8 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.24|P-value:2.43E-8 ID:SGTC_1158|Compound:4092-0494|FD-Score:2.99|P-value:9.18E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.54|P-value:1.19E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.78|P-value:4.16E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:5.03|P-value:8.13E-8 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-4.07|P-value:1.20E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.86|P-value:2.94E-5 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.09|P-value:6.86E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.38|P-value:2.15E-4 ID:SGTC_1663|Compound:st014140|FD-Score:3.01|P-value:8.59E-4 ID:SGTC_1892|Compound:st058411|FD-Score:-3.25|P-value:3.74E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.65|P-value:7.13E-5 ID:SGTC_2000|Compound:st075254|FD-Score:-3.25|P-value:3.80E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.03|P-value:7.95E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.05|P-value:7.35E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.48|P-value:1.45E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.27|P-value:3.28E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.82|P-value:3.50E-5 ID:SGTC_2375|Compound:9072683|FD-Score:3.18|P-value:4.65E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.93|P-value:2.15E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.52|P-value:1.25E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-3.87|P-value:2.98E-5 ID:SGTC_2463|Compound:5473429|FD-Score:3.36|P-value:2.33E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-4.20|P-value:6.49E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.41|P-value:2.23E-6 ID:SGTC_2524|Compound:avocatin b|FD-Score:4.31|P-value:3.55E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:4.91|P-value:1.66E-7 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.60|P-value:9.02E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.68|P-value:6.35E-5 ID:SGTC_2878|Compound:9043973|FD-Score:3.17|P-value:4.76E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.37|P-value:2.24E-4 ID:SGTC_2886|Compound:9058944|FD-Score:3.23|P-value:3.80E-4 ID:SGTC_2897|Compound:9048598|FD-Score:-3.37|P-value:2.33E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.64|P-value:7.98E-5 ID:SGTC_3083|Compound:9117465|FD-Score:3.04|P-value:7.85E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-4.85|P-value:2.44E-7 ID:SGTC_3132|Compound:9126706|FD-Score:4.17|P-value:7.22E-6 ID:SGTC_3159|Compound:9100096|FD-Score:-2.98|P-value:9.99E-4 ID:SGTC_3216|Compound:9129716|FD-Score:3.67|P-value:6.72E-5 ID:SGTC_3310|Compound:9130229|FD-Score:3.05|P-value:7.51E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.15|P-value:5.17E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.39|P-value:2.08E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.15|P-value:5.48E-4 ID:SGTC_65|Compound:0798-0905|FD-Score:3.13|P-value:5.66E-4 ID:SGTC_132|Compound:0417-1715|FD-Score:-3.03|P-value:8.38E-4 ID:SGTC_134|Compound:0269-0018|FD-Score:-3.53|P-value:1.23E-4 ID:SGTC_151|Compound:3013-0144|FD-Score:3.05|P-value:7.46E-4 ID:SGTC_274|Compound:sirtinol|FD-Score:3.86|P-value:2.94E-5 ID:SGTC_277|Compound:nystatin|FD-Score:-3.02|P-value:8.72E-4 ID:SGTC_350|Compound:k064-0020|FD-Score:3.39|P-value:2.07E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.37|P-value:2.23E-4 ID:SGTC_779|Compound:1360-0071|FD-Score:3.35|P-value:2.47E-4 ID:SGTC_787|Compound:4152-0075|FD-Score:-3.61|P-value:9.14E-5 ID:SGTC_836|Compound:0330-0104|FD-Score:-3.32|P-value:2.87E-4 ID:SGTC_838|Compound:0109-0203|FD-Score:-3.10|P-value:6.51E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:5.02|P-value:8.55E-8 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:5.24|P-value:2.43E-8 ID:SGTC_1158|Compound:4092-0494|FD-Score:2.99|P-value:9.18E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:-3.54|P-value:1.19E-4 ID:SGTC_1244|Compound:0416-0015|FD-Score:3.78|P-value:4.16E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:5.03|P-value:8.13E-8 ID:SGTC_1349|Compound:flufenamic acid|FD-Score:-4.07|P-value:1.20E-5 ID:SGTC_1350|Compound:1495-0454|FD-Score:3.86|P-value:2.94E-5 ID:SGTC_1362|Compound:1611-0397|FD-Score:-3.09|P-value:6.86E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.38|P-value:2.15E-4 ID:SGTC_1663|Compound:st014140|FD-Score:3.01|P-value:8.59E-4 ID:SGTC_1892|Compound:st058411|FD-Score:-3.25|P-value:3.74E-4 ID:SGTC_1903|Compound:st060211|FD-Score:3.65|P-value:7.13E-5 ID:SGTC_2000|Compound:st075254|FD-Score:-3.25|P-value:3.80E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.03|P-value:7.95E-4 ID:SGTC_2073|Compound:5212005|FD-Score:3.05|P-value:7.35E-4 ID:SGTC_2256|Compound:7954252|FD-Score:3.48|P-value:1.45E-4 ID:SGTC_2264|Compound:7948757|FD-Score:3.27|P-value:3.28E-4 ID:SGTC_2357|Compound:9067248|FD-Score:3.82|P-value:3.50E-5 ID:SGTC_2375|Compound:9072683|FD-Score:3.18|P-value:4.65E-4 ID:SGTC_2380|Compound:9035439|FD-Score:3.93|P-value:2.15E-5 ID:SGTC_2398|Compound:5228213|FD-Score:3.52|P-value:1.25E-4 ID:SGTC_2399|Compound:5884133|FD-Score:-3.87|P-value:2.98E-5 ID:SGTC_2463|Compound:5473429|FD-Score:3.36|P-value:2.33E-4 ID:SGTC_2475|Compound:5812728|FD-Score:-4.20|P-value:6.49E-6 ID:SGTC_2514|Compound:salinomycin|FD-Score:4.41|P-value:2.23E-6 ID:SGTC_2524|Compound:avocatin b|FD-Score:4.31|P-value:3.55E-6 ID:SGTC_2620|Compound:irigenin|FD-Score:4.91|P-value:1.66E-7 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:3.60|P-value:9.02E-5 ID:SGTC_2627|Compound:acetyl isogambogic acid|FD-Score:3.68|P-value:6.35E-5 ID:SGTC_2878|Compound:9043973|FD-Score:3.17|P-value:4.76E-4 ID:SGTC_2885|Compound:9057732|FD-Score:3.37|P-value:2.24E-4 ID:SGTC_2886|Compound:9058944|FD-Score:3.23|P-value:3.80E-4 ID:SGTC_2897|Compound:9048598|FD-Score:-3.37|P-value:2.33E-4 ID:SGTC_2990|Compound:9074105|FD-Score:-3.64|P-value:7.98E-5 ID:SGTC_3083|Compound:9117465|FD-Score:3.04|P-value:7.85E-4 ID:SGTC_3130|Compound:9127414|FD-Score:-4.85|P-value:2.44E-7 ID:SGTC_3132|Compound:9126706|FD-Score:4.17|P-value:7.22E-6 ID:SGTC_3159|Compound:9100096|FD-Score:-2.98|P-value:9.99E-4 ID:SGTC_3216|Compound:9129716|FD-Score:3.67|P-value:6.72E-5 ID:SGTC_3310|Compound:9130229|FD-Score:3.05|P-value:7.51E-4 ID:SGTC_3313|Compound:9133968|FD-Score:3.15|P-value:5.17E-4 ID:SGTC_3314|Compound:9135860|FD-Score:3.39|P-value:2.08E-4 ID:SGTC_3325|Compound:9138760|FD-Score:-3.15|P-value:5.48E-4

Top fitness defect scores for YHL030W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1083 didemethylchlorpromazine sulfoxide 5.24 707.4 nM 2.43E-8 redox potentiating 43 7.85E-8
2 SGTC_1346 1488-2224 5.03 62.4 µM 8.13E-8 13 2.41E-7
3 SGTC_1013 4112-3890 5.02 241.0 µM 8.55E-8 TSC3-RPN4 16 2.52E-7
4 SGTC_2620 irigenin 4.91 100.0 µM 1.66E-7 14 4.66E-7
5 SGTC_2514 salinomycin 4.41 42.7 µM 2.23E-6 32 5.19E-6
6 SGTC_2524 avocatin b 4.31 10.6 µM 3.55E-6 20 7.99E-6
7 SGTC_3132 9126706 4.17 49.5 µM 7.22E-6 16 1.54E-5
8 SGTC_2380 9035439 3.93 200.0 µM 2.15E-5 endomembrane recycling 23 4.24E-5
9 SGTC_274 sirtinol 3.86 40.1 µM 2.94E-5 22 5.69E-5
10 SGTC_1350 1495-0454 3.86 146.0 µM 2.94E-5 32 5.69E-5
11 SGTC_2357 9067248 3.82 200.0 µM 3.50E-5 23 6.67E-5
12 SGTC_1244 0416-0015 3.78 52.5 µM 4.16E-5 ERG2 39 7.83E-5
13 SGTC_2627 acetyl isogambogic acid 3.68 100.0 µM 6.35E-5 TSC3-RPN4 51 1.16E-4
14 SGTC_3216 9129716 3.67 49.5 µM 6.72E-5 RPP1 & pyrimidine depletion 30 1.22E-4
15 SGTC_1903 st060211 3.65 78.3 µM 7.13E-5 38 1.29E-4
16 SGTC_2621 lithochol-11-enic acid 3.60 100.0 µM 9.02E-5 39 1.61E-4
17 SGTC_2398 5228213 3.52 48.8 µM 1.25E-4 iron homeostasis 54 2.17E-4
18 SGTC_2256 7954252 3.48 200.0 µM 1.45E-4 mitochondrial processes 11 2.50E-4
19 SGTC_350 k064-0020 3.39 19.8 µM 2.07E-4 heme requiring 42 3.47E-4
20 SGTC_3314 9135860 3.39 59.4 µM 2.08E-4 32 3.48E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.186 1.23E-27 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C
0.149 3.78E-18 YPL252C YAH1 het Ferredoxin of the mitochondrial matrix required for formation of cellular iron-sulfur proteins; involved in heme A biosynthesis; homologous to human adrenodoxin
0.148 8.21E-18 YDR431W_d YDR431W_d hom Dubious ORF unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.097 1.57E-8 YLR228C ECM22 hom Sterol regulatory element binding protein; regulates transcription of sterol biosynthetic genes; contains Zn[2]-Cys[6] binuclear cluster; relocates from intracellular membranes to perinuclear foci on sterol depletion; ECM22 has a paralog, UPC2, that arose from the whole genome duplication
0.097 1.59E-8 YNL203C_d YNL203C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.095 3.70E-8 YAL054C ACS1 hom Acetyl-coA synthetase isoform which, along with Acs2p, is the nuclear source of acetyl-coA for histone acetylation; expressed during growth on nonfermentable carbon sources and under aerobic conditions
0.092 8.09E-8 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.092 9.21E-8 YML066C SMA2 hom Meiosis-specific prospore membrane protein; required to produce bending force necessary for proper assembly of the prospore membrane during sporulation
0.091 1.45E-7 YBR139W YBR139W hom Putative serine type carboxypeptidase with a role in phytochelatin synthesis; green fluorescent protein (GFP)-fusion protein localizes to the vacuole; expression induced by nitrogen limitation in a GLN3, GAT1-independent manner
0.090 1.54E-7 YNL291C MID1 hom N-glycosylated integral membrane protein of the ER membrane and plasma membrane, functions as a stretch-activated Ca2+-permeable cation channel required for Ca2+ influx stimulated by pheromone; interacts with Cch1p; forms an oligomer
0.090 1.52E-7 YFL015C_d YFL015C_d hom Dubious open reading frame unlikely to encode a protein; partially overlaps dubious ORF YFL015W-A; YFL015C is not an essential gene
0.088 3.41E-7 YNL007C SIS1 het Type II HSP40 co-chaperone that interacts with the HSP70 protein Ssa1p; not functionally redundant with Ydj1p due to due to substrate specificity; has similarity to bacterial DnaJ proteins; protein abundance increases in response to DNA replication stress
0.086 5.82E-7 YDL034W_d YDL034W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps with verified gene GPR1/YDL035C; YDL034W is not an essential gene
0.084 1.22E-6 YNR026C SEC12 het Guanine nucleotide exchange factor (GEF), activates Sar1p by catalyzing the exchange of GDP for GTP; required for the initiation of COPII vesicle formation in ER to Golgi transport; glycosylated integral membrane protein of the ER
0.083 1.50E-6 YJR038C_d YJR038C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data