YHL033C / RPL8A

Ribosomal 60S subunit protein L8A; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8A has a paralog, RPL8B, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL033C / RPL8A

Click on Significant Values for Screen Details ID:SGTC_18|Compound:0111-0177|FD-Score:5.99|P-value:2.50E-8 ID:SGTC_72|Compound:0744-0168|FD-Score:5.41|P-value:3.96E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:4.81|P-value:5.38E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:4.36|P-value:3.19E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:-4.28|P-value:1.06E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.75|P-value:1.86E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.00|P-value:6.82E-6 ID:SGTC_369|Compound:0957-0393|FD-Score:3.49|P-value:6.37E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:4.05|P-value:9.87E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:4.83|P-value:4.90E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:4.10|P-value:8.22E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:4.48|P-value:2.01E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.63|P-value:4.66E-7 ID:SGTC_959|Compound:1222-0044|FD-Score:5.23|P-value:9.04E-7 ID:SGTC_972|Compound:1319-0219|FD-Score:5.47|P-value:3.01E-7 ID:SGTC_979|Compound:1426-2828|FD-Score:5.51|P-value:2.53E-7 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.71|P-value:3.09E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.61|P-value:1.19E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:-4.62|P-value:3.02E-5 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:4.58|P-value:1.34E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:5.00|P-value:2.40E-6 ID:SGTC_1441|Compound:4106-0040|FD-Score:4.16|P-value:6.74E-5 ID:SGTC_1635|Compound:st007035|FD-Score:-4.60|P-value:3.38E-5 ID:SGTC_1716|Compound:st033144|FD-Score:-6.09|P-value:5.14E-8 ID:SGTC_1724|Compound:st037281|FD-Score:9.53|P-value:3.81E-18 ID:SGTC_1737|Compound:st035557|FD-Score:3.65|P-value:3.79E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.39|P-value:8.59E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-4.32|P-value:9.28E-5 ID:SGTC_1846|Compound:st056244|FD-Score:5.97|P-value:2.73E-8 ID:SGTC_1847|Compound:st056231|FD-Score:-4.27|P-value:1.11E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:5.47|P-value:3.01E-7 ID:SGTC_1860|Compound:st056228|FD-Score:5.26|P-value:7.71E-7 ID:SGTC_1861|Compound:st057647|FD-Score:3.86|P-value:1.87E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-4.99|P-value:7.12E-6 ID:SGTC_2132|Compound:5317594|FD-Score:-4.04|P-value:2.41E-4 ID:SGTC_2268|Compound:7933382|FD-Score:3.49|P-value:6.36E-4 ID:SGTC_2285|Compound:7945142|FD-Score:4.89|P-value:3.90E-6 ID:SGTC_2291|Compound:7917025|FD-Score:-3.84|P-value:4.61E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.08|P-value:8.72E-5 ID:SGTC_2557|Compound:derrustone|FD-Score:3.49|P-value:6.28E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.52|P-value:5.74E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-5.32|P-value:1.82E-6 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.59|P-value:4.69E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-4.72|P-value:2.10E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.41|P-value:9.19E-12 ID:SGTC_2781|Compound:5567276|FD-Score:-5.13|P-value:4.06E-6 ID:SGTC_2980|Compound:9014342|FD-Score:3.45|P-value:7.08E-4 ID:SGTC_2985|Compound:9022375|FD-Score:5.39|P-value:4.28E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.88|P-value:1.79E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-5.93|P-value:1.16E-7 ID:SGTC_3198|Compound:9112019|FD-Score:-3.68|P-value:7.70E-4 ID:SGTC_18|Compound:0111-0177|FD-Score:5.99|P-value:2.50E-8 ID:SGTC_72|Compound:0744-0168|FD-Score:5.41|P-value:3.96E-7 ID:SGTC_87|Compound:0100-0052|FD-Score:4.81|P-value:5.38E-6 ID:SGTC_118|Compound:0083-0097|FD-Score:4.36|P-value:3.19E-5 ID:SGTC_171|Compound:k064-0041|FD-Score:-4.28|P-value:1.06E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-4.75|P-value:1.86E-5 ID:SGTC_310|Compound:1273-0058|FD-Score:-5.00|P-value:6.82E-6 ID:SGTC_369|Compound:0957-0393|FD-Score:3.49|P-value:6.37E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:4.05|P-value:9.87E-5 ID:SGTC_506|Compound:n9-isopropylolomoucine|FD-Score:4.83|P-value:4.90E-6 ID:SGTC_537|Compound:1334-0398|FD-Score:4.10|P-value:8.22E-5 ID:SGTC_791|Compound:1348-1292|FD-Score:4.48|P-value:2.01E-5 ID:SGTC_893|Compound:0958-0369|FD-Score:-5.63|P-value:4.66E-7 ID:SGTC_959|Compound:1222-0044|FD-Score:5.23|P-value:9.04E-7 ID:SGTC_972|Compound:1319-0219|FD-Score:5.47|P-value:3.01E-7 ID:SGTC_979|Compound:1426-2828|FD-Score:5.51|P-value:2.53E-7 ID:SGTC_1006|Compound:4099-6547|FD-Score:3.71|P-value:3.09E-4 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.61|P-value:1.19E-5 ID:SGTC_1057|Compound:3448-5381|FD-Score:-4.62|P-value:3.02E-5 ID:SGTC_1099|Compound:5-nonyloxytryptamine|FD-Score:4.58|P-value:1.34E-5 ID:SGTC_1368|Compound:1852-0141|FD-Score:5.00|P-value:2.40E-6 ID:SGTC_1441|Compound:4106-0040|FD-Score:4.16|P-value:6.74E-5 ID:SGTC_1635|Compound:st007035|FD-Score:-4.60|P-value:3.38E-5 ID:SGTC_1716|Compound:st033144|FD-Score:-6.09|P-value:5.14E-8 ID:SGTC_1724|Compound:st037281|FD-Score:9.53|P-value:3.81E-18 ID:SGTC_1737|Compound:st035557|FD-Score:3.65|P-value:3.79E-4 ID:SGTC_1787|Compound:fluconazole|FD-Score:3.39|P-value:8.59E-4 ID:SGTC_1829|Compound:berberine|FD-Score:-4.32|P-value:9.28E-5 ID:SGTC_1846|Compound:st056244|FD-Score:5.97|P-value:2.73E-8 ID:SGTC_1847|Compound:st056231|FD-Score:-4.27|P-value:1.11E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:5.47|P-value:3.01E-7 ID:SGTC_1860|Compound:st056228|FD-Score:5.26|P-value:7.71E-7 ID:SGTC_1861|Compound:st057647|FD-Score:3.86|P-value:1.87E-4 ID:SGTC_2042|Compound:p-Dimethylaminoacetophenone|FD-Score:-4.99|P-value:7.12E-6 ID:SGTC_2132|Compound:5317594|FD-Score:-4.04|P-value:2.41E-4 ID:SGTC_2268|Compound:7933382|FD-Score:3.49|P-value:6.36E-4 ID:SGTC_2285|Compound:7945142|FD-Score:4.89|P-value:3.90E-6 ID:SGTC_2291|Compound:7917025|FD-Score:-3.84|P-value:4.61E-4 ID:SGTC_2540|Compound:epiandrosterone|FD-Score:4.08|P-value:8.72E-5 ID:SGTC_2557|Compound:derrustone|FD-Score:3.49|P-value:6.28E-4 ID:SGTC_2603|Compound:homopterocarpin|FD-Score:3.52|P-value:5.74E-4 ID:SGTC_2604|Compound:4'-hydroxychalcone|FD-Score:-5.32|P-value:1.82E-6 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.59|P-value:4.69E-4 ID:SGTC_2632|Compound:3-hydroxy-4-(succin-2-yl)-caryolane delta-lactone|FD-Score:-4.72|P-value:2.10E-5 ID:SGTC_2688|Compound:kanamycin b|FD-Score:7.41|P-value:9.19E-12 ID:SGTC_2781|Compound:5567276|FD-Score:-5.13|P-value:4.06E-6 ID:SGTC_2980|Compound:9014342|FD-Score:3.45|P-value:7.08E-4 ID:SGTC_2985|Compound:9022375|FD-Score:5.39|P-value:4.28E-7 ID:SGTC_2996|Compound:9066192|FD-Score:3.88|P-value:1.79E-4 ID:SGTC_3152|Compound:9099253|FD-Score:-5.93|P-value:1.16E-7 ID:SGTC_3198|Compound:9112019|FD-Score:-3.68|P-value:7.70E-4

Top fitness defect scores for YHL033C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1724 st037281 9.53 8.3 µM 3.81E-18 1 8.16E-22
2 SGTC_2688 kanamycin b 7.41 96.7 µM 9.19E-12 calcium & mitochondrial duress 91 6.32E-14
3 SGTC_18 0111-0177 5.99 500.0 nM 2.50E-8 19 1.06E-9
4 SGTC_1846 st056244 5.97 14.8 µM 2.73E-8 4 1.18E-9
5 SGTC_979 1426-2828 5.51 121.0 µM 2.53E-7 5 1.80E-8
6 SGTC_1858 4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione 5.47 62.6 µM 3.01E-7 TSC3-RPN4 5 2.23E-8
7 SGTC_972 1319-0219 5.47 195.0 µM 3.01E-7 6 2.23E-8
8 SGTC_72 0744-0168 5.41 198.4 µM 3.96E-7 RPP1 & pyrimidine depletion 10 3.12E-8
9 SGTC_2985 9022375 5.39 71.4 µM 4.28E-7 RPP1 & pyrimidine depletion 9 3.43E-8
10 SGTC_1860 st056228 5.26 74.6 µM 7.71E-7 3 7.03E-8
11 SGTC_959 1222-0044 5.23 107.0 µM 9.04E-7 6 8.54E-8
12 SGTC_1368 1852-0141 5.00 284.0 µM 2.40E-6 ERG2 21 2.81E-7
13 SGTC_2285 7945142 4.89 200.0 µM 3.90E-6 6 5.08E-7
14 SGTC_506 n9-isopropylolomoucine 4.83 153.0 µM 4.90E-6 4 6.71E-7
15 SGTC_87 0100-0052 4.81 35.5 µM 5.38E-6 62 7.52E-7
16 SGTC_1049 3226-0134 4.61 66.0 µM 1.19E-5 17 1.97E-6
17 SGTC_1099 5-nonyloxytryptamine 4.58 2.9 µM 1.34E-5 9 2.29E-6
18 SGTC_791 1348-1292 4.48 225.0 µM 2.01E-5 25 3.73E-6
19 SGTC_118 0083-0097 4.36 135.3 µM 3.19E-5 translation 95 6.55E-6
20 SGTC_1441 4106-0040 4.16 13.0 µM 6.74E-5 8 1.62E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.174 4.13E-24 YLL045C RPL8B hom Ribosomal 60S subunit protein L8B; mutation results in decreased amounts of free 60S subunits; homologous to mammalian ribosomal protein L7A, no bacterial homolog; RPL8B has a paralog, RPL8A, that arose from the whole genome duplication
0.162 3.06E-21 YNL067W RPL9B hom Ribosomal 60S subunit protein L9B; nearly identical to paralog Rpl9Ap; homologous to mammalian ribosomal protein L9 and bacterial L6
0.148 7.48E-18 YDR417C_d YDR417C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF RPL12B/YDR418W
0.146 1.93E-17 YML073C RPL6A hom Ribosomal 60S subunit protein L6A; N-terminally acetylated; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6A has a paralog, RPL6B, that arose from the whole genome duplication
0.146 2.13E-17 YNL248C RPA49 hom RNA polymerase I subunit A49
0.141 2.43E-16 YEL054C RPL12A hom Ribosomal 60S subunit protein L12A; rpl12a rpl12b double mutant exhibits slow growth and slow translation; homologous to mammalian ribosomal protein L12 and bacterial L11; RPL12A has a paralog, RPL12B, that arose from the whole genome duplication
0.119 4.34E-12 YDR101C ARX1 hom Shuttling pre-60S factor; involved in the biogenesis of ribosomal large subunit biogenesis; interacts directly with Alb1; responsible for Tif6 recycling defects in absence of Rei1; associated with the ribosomal export complex
0.118 6.55E-12 YBR287W_p YBR287W_p hom Protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the ER; YBR287W is not an essential gene
0.118 8.22E-12 YFR031C-A RPL2A hom Ribosomal 60S subunit protein L2A; homologous to mammalian ribosomal protein L2 and bacterial L2; RPL2A has a paralog, RPL2B, that arose from the whole genome duplication
0.117 9.99E-12 YPL125W KAP120 hom Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p
0.115 2.03E-11 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.109 2.51E-10 YDL081C RPP1A hom Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
0.107 5.40E-10 YCR095C_p OCA4_p hom Cytoplasmic protein required for replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.107 5.41E-10 YCR075W-A_p YCR075W-A_p hom Putative protein of unknown function; identified by homology to Ashbya gossypii; YCR075W-A has a paralog, YNR034W-A, that arose from the whole genome duplication
0.107 5.91E-10 YOR312C RPL20B hom Ribosomal 60S subunit protein L20B; homologous to mammalian ribosomal protein L18A, no bacterial homolog; RPL20B has a paralog, RPL20A, that arose from the whole genome duplication