YHL035C / VMR1

Vacuolar membrane protein involved in multiple drug resistance and metal sensitivity; ATP-binding cassette (ABC) family member involved in drug transport; potential Cdc28p substrate; induced under respiratory conditions

Zygosity: Homozygous strain
fixedexpanded
Profile for YHL035C / VMR1

Click on Significant Values for Screen Details ID:SGTC_4|Compound:0960-0234|FD-Score:4.31|P-value:1.12E-5 ID:SGTC_5|Compound:0352-0601|FD-Score:4.95|P-value:5.52E-7 ID:SGTC_51|Compound:1013-0126|FD-Score:-3.50|P-value:2.53E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.53|P-value:2.63E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.17|P-value:9.13E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.82|P-value:8.49E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:3.62|P-value:1.85E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.96|P-value:4.86E-5 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:3.74|P-value:1.18E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.73|P-value:1.23E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.62|P-value:1.88E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:3.18|P-value:8.80E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:4.47|P-value:5.35E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:3.72|P-value:1.27E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:4.38|P-value:8.26E-6 ID:SGTC_1047|Compound:2043-3044|FD-Score:-4.39|P-value:6.64E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.72|P-value:1.72E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:-4.42|P-value:5.98E-6 ID:SGTC_1055|Compound:3448-0672|FD-Score:-3.56|P-value:2.09E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.16|P-value:9.45E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.01|P-value:4.04E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.35|P-value:4.45E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.45|P-value:3.48E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-4.78|P-value:1.07E-6 ID:SGTC_1547|Compound:harmine|FD-Score:3.16|P-value:9.58E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.00|P-value:4.15E-5 ID:SGTC_1700|Compound:st030840|FD-Score:-4.44|P-value:5.26E-6 ID:SGTC_1935|Compound:st074532|FD-Score:3.28|P-value:6.23E-4 ID:SGTC_2033|Compound:5192550|FD-Score:4.92|P-value:6.40E-7 ID:SGTC_2047|Compound:4141938|FD-Score:-3.47|P-value:2.91E-4 ID:SGTC_2193|Compound:5925765|FD-Score:-4.50|P-value:4.02E-6 ID:SGTC_2211|Compound:7117706|FD-Score:3.88|P-value:6.75E-5 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.30|P-value:5.89E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.94|P-value:5.34E-5 ID:SGTC_2283|Compound:7944793|FD-Score:3.93|P-value:5.50E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-4.30|P-value:1.02E-5 ID:SGTC_2349|Compound:9023730|FD-Score:3.44|P-value:3.57E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.49|P-value:2.98E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.09|P-value:2.86E-5 ID:SGTC_2511|Compound:berberine|FD-Score:6.76|P-value:1.32E-11 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.24|P-value:7.21E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-3.48|P-value:2.72E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.30|P-value:5.23E-4 ID:SGTC_2934|Compound:9036370|FD-Score:4.12|P-value:2.56E-5 ID:SGTC_2936|Compound:9038235|FD-Score:-7.23|P-value:4.20E-13 ID:SGTC_2939|Compound:9044578|FD-Score:3.64|P-value:1.74E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.65|P-value:1.45E-4 ID:SGTC_3187|Compound:9109394|FD-Score:-3.57|P-value:2.00E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-3.29|P-value:5.49E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.19|P-value:7.73E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.71|P-value:1.34E-4 ID:SGTC_3309|Compound:9129340|FD-Score:3.49|P-value:3.00E-4 ID:SGTC_3319|Compound:9138450|FD-Score:4.49|P-value:4.86E-6 ID:SGTC_3320|Compound:9137005|FD-Score:-3.15|P-value:8.79E-4 ID:SGTC_3335|Compound:9143315|FD-Score:5.01|P-value:3.99E-7 ID:SGTC_4|Compound:0960-0234|FD-Score:4.31|P-value:1.12E-5 ID:SGTC_5|Compound:0352-0601|FD-Score:4.95|P-value:5.52E-7 ID:SGTC_51|Compound:1013-0126|FD-Score:-3.50|P-value:2.53E-4 ID:SGTC_69|Compound:0096-0274|FD-Score:3.53|P-value:2.63E-4 ID:SGTC_94|Compound:0334-0113|FD-Score:3.17|P-value:9.13E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.82|P-value:8.49E-5 ID:SGTC_184|Compound:1494-0393|FD-Score:3.62|P-value:1.85E-4 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:3.96|P-value:4.86E-5 ID:SGTC_494|Compound:Boc-Gly-Val-Val-CHO|FD-Score:3.74|P-value:1.18E-4 ID:SGTC_507|Compound:nimesulide|FD-Score:3.73|P-value:1.23E-4 ID:SGTC_798|Compound:1287-0182|FD-Score:3.62|P-value:1.88E-4 ID:SGTC_897|Compound:1000-0139|FD-Score:3.18|P-value:8.80E-4 ID:SGTC_942|Compound:3253-1115|FD-Score:4.47|P-value:5.35E-6 ID:SGTC_957|Compound:1187-1561|FD-Score:3.72|P-value:1.27E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:4.38|P-value:8.26E-6 ID:SGTC_1047|Compound:2043-3044|FD-Score:-4.39|P-value:6.64E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:4.72|P-value:1.72E-6 ID:SGTC_1050|Compound:3232-1156|FD-Score:-4.42|P-value:5.98E-6 ID:SGTC_1055|Compound:3448-0672|FD-Score:-3.56|P-value:2.09E-4 ID:SGTC_1056|Compound:3448-1962|FD-Score:3.16|P-value:9.45E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.01|P-value:4.04E-5 ID:SGTC_1204|Compound:4049-0315|FD-Score:-3.35|P-value:4.45E-4 ID:SGTC_1391|Compound:3442-0225|FD-Score:3.45|P-value:3.48E-4 ID:SGTC_1504|Compound:k297-0033|FD-Score:-4.78|P-value:1.07E-6 ID:SGTC_1547|Compound:harmine|FD-Score:3.16|P-value:9.58E-4 ID:SGTC_1632|Compound:st005650|FD-Score:4.00|P-value:4.15E-5 ID:SGTC_1700|Compound:st030840|FD-Score:-4.44|P-value:5.26E-6 ID:SGTC_1935|Compound:st074532|FD-Score:3.28|P-value:6.23E-4 ID:SGTC_2033|Compound:5192550|FD-Score:4.92|P-value:6.40E-7 ID:SGTC_2047|Compound:4141938|FD-Score:-3.47|P-value:2.91E-4 ID:SGTC_2193|Compound:5925765|FD-Score:-4.50|P-value:4.02E-6 ID:SGTC_2211|Compound:7117706|FD-Score:3.88|P-value:6.75E-5 ID:SGTC_2247|Compound:cerulenin|FD-Score:3.30|P-value:5.89E-4 ID:SGTC_2266|Compound:7929956|FD-Score:3.94|P-value:5.34E-5 ID:SGTC_2283|Compound:7944793|FD-Score:3.93|P-value:5.50E-5 ID:SGTC_2340|Compound:7987427|FD-Score:-4.30|P-value:1.02E-5 ID:SGTC_2349|Compound:9023730|FD-Score:3.44|P-value:3.57E-4 ID:SGTC_2424|Compound:5537928|FD-Score:3.49|P-value:2.98E-4 ID:SGTC_2425|Compound:5628681|FD-Score:4.09|P-value:2.86E-5 ID:SGTC_2511|Compound:berberine|FD-Score:6.76|P-value:1.32E-11 ID:SGTC_2562|Compound:3',7'-dimethoxyflavone|FD-Score:3.24|P-value:7.21E-4 ID:SGTC_2808|Compound:7946468|FD-Score:-3.48|P-value:2.72E-4 ID:SGTC_2834|Compound:9001755|FD-Score:-3.30|P-value:5.23E-4 ID:SGTC_2934|Compound:9036370|FD-Score:4.12|P-value:2.56E-5 ID:SGTC_2936|Compound:9038235|FD-Score:-7.23|P-value:4.20E-13 ID:SGTC_2939|Compound:9044578|FD-Score:3.64|P-value:1.74E-4 ID:SGTC_2940|Compound:9060638|FD-Score:-3.65|P-value:1.45E-4 ID:SGTC_3187|Compound:9109394|FD-Score:-3.57|P-value:2.00E-4 ID:SGTC_3189|Compound:9110682|FD-Score:-3.29|P-value:5.49E-4 ID:SGTC_3199|Compound:9111318|FD-Score:-3.19|P-value:7.73E-4 ID:SGTC_3234|Compound:9132280|FD-Score:3.71|P-value:1.34E-4 ID:SGTC_3309|Compound:9129340|FD-Score:3.49|P-value:3.00E-4 ID:SGTC_3319|Compound:9138450|FD-Score:4.49|P-value:4.86E-6 ID:SGTC_3320|Compound:9137005|FD-Score:-3.15|P-value:8.79E-4 ID:SGTC_3335|Compound:9143315|FD-Score:5.01|P-value:3.99E-7

Top fitness defect scores for YHL035C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2511 berberine 6.76 10.5 µM 1.32E-11 60S ribosome export 2 6.69E-12
2 SGTC_3335 9143315 5.01 29.6 µM 3.99E-7 fatty acid desaturase (OLE1) 3 2.68E-7
3 SGTC_5 0352-0601 4.95 109.9 µM 5.52E-7 ERG2 9 3.74E-7
4 SGTC_2033 5192550 4.92 145.0 µM 6.40E-7 5 4.34E-7
5 SGTC_1049 3226-0134 4.72 66.0 µM 1.72E-6 15 1.20E-6
6 SGTC_3319 9138450 4.49 16.8 µM 4.86E-6 8 3.49E-6
7 SGTC_942 3253-1115 4.47 227.0 µM 5.35E-6 17 3.85E-6
8 SGTC_960 1222-0186 4.38 7.7 µM 8.26E-6 22 6.02E-6
9 SGTC_4 0960-0234 4.31 25.7 µM 1.12E-5 Golgi 9 8.25E-6
10 SGTC_2934 9036370 4.12 36.9 µM 2.56E-5 29 1.92E-5
11 SGTC_2425 5628681 4.09 7.8 µM 2.86E-5 20 2.15E-5
12 SGTC_1196 1588-0294 4.01 184.0 µM 4.04E-5 11 3.07E-5
13 SGTC_1632 st005650 4.00 90.0 µM 4.15E-5 29 3.16E-5
14 SGTC_436 n,n-dimethylsphingosine 3.96 3.3 µM 4.86E-5 28 3.72E-5
15 SGTC_2266 7929956 3.94 200.0 µM 5.34E-5 36 4.09E-5
16 SGTC_2283 7944793 3.93 200.0 µM 5.50E-5 43 4.22E-5
17 SGTC_2211 7117706 3.88 200.0 µM 6.75E-5 14 5.20E-5
18 SGTC_145 3737-0113 3.82 38.4 µM 8.49E-5 DNA intercalators 78 6.58E-5
19 SGTC_494 Boc-Gly-Val-Val-CHO 3.74 140.0 µM 1.18E-4 10 9.26E-5
20 SGTC_507 nimesulide 3.73 162.0 µM 1.23E-4 22 9.61E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.097 1.72E-8 YHR020W YHR020W het Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments
0.089 2.69E-7 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.085 8.48E-7 YLR231C BNA5 hom Kynureninase, required for the de novo biosynthesis of NAD from tryptophan via kynurenine; expression regulated by Hst1p
0.083 1.69E-6 YDR133C_d YDR133C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps YDR134C
0.082 1.87E-6 YKR040C_d YKR040C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the uncharacterized ORF YKR041W
0.082 2.10E-6 YKR064W OAF3 hom Putative transcriptional repressor with Zn(2)-Cys(6) finger; negatively regulates transcription in response to oleate levels, based on mutant phenotype and localization to oleate-responsive promoters; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; forms nuclear foci upon DNA replication stress
0.078 6.47E-6 YBR087W RFC5 het Subunit of heteropentameric Replication factor C (RF-C), which is a DNA binding protein and ATPase that acts as a clamp loader of the proliferating cell nuclear antigen (PCNA) processivity factor for DNA polymerases delta and epsilon
0.078 6.54E-6 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.077 7.19E-6 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.076 1.09E-5 YDR211W GCD6 het Catalytic epsilon subunit of the translation initiation factor eIF2B; eIF2B is the guanine-nucleotide exchange factor for eIF2; activity subsequently regulated by phosphorylated eIF2; first identified as a negative regulator of GCN4 expression; forms cytoplasmic foci upon DNA replication stress
0.072 2.88E-5 YBR055C PRP6 het Splicing factor, component of the U4/U6-U5 snRNP complex
0.072 3.10E-5 YPL240C HSP82 hom Hsp90 chaperone; redundant in function with Hsc82p; required for pheromone signaling, negative regulation of Hsf1p; docks with Tom70p for mitochondrial preprotein delivery; promotes telomerase DNA binding, nucleotide addition; protein abundance increases in response to DNA replication stress; contains two acid-rich unstructured regions that promote solubility of chaperone-substrate complexes; HSP82 has a paralog, HSC82, that arose from the whole genome duplication
0.071 3.73E-5 YDR528W HLR1 hom Protein involved in regulation of cell wall composition and integrity and response to osmotic stress; overproduction suppresses a lysis sensitive PKC mutation; similar to Lre1p, which functions antagonistically to protein kinase A;
HLR1 has a paralog, LRE1, that arose from the whole genome duplication
0.071 4.07E-5 YKL202W_d YKL202W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.071 4.28E-5 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar