YHR010W / RPL27A

Ribosomal 60S subunit protein L27A; homologous to mammalian ribosomal protein L27, no bacterial homolog; RPL27A has a paralog, RPL27B, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR010W / RPL27A

Click on Significant Values for Screen Details ID:SGTC_118|Compound:0083-0097|FD-Score:6.26|P-value:8.51E-11 ID:SGTC_123|Compound:0083-0120|FD-Score:4.61|P-value:1.08E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:4.11|P-value:1.15E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:3.15|P-value:5.43E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:4.03|P-value:1.67E-5 ID:SGTC_269|Compound:exalamide|FD-Score:3.83|P-value:3.96E-5 ID:SGTC_276|Compound:colchicine|FD-Score:-4.39|P-value:8.11E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-7.17|P-value:6.12E-13 ID:SGTC_581|Compound:0958-0271|FD-Score:4.01|P-value:1.80E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:4.42|P-value:2.84E-6 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.60|P-value:2.22E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.42|P-value:2.01E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:5.27|P-value:3.42E-8 ID:SGTC_831|Compound:k061-1017|FD-Score:5.42|P-value:1.42E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:5.72|P-value:2.46E-9 ID:SGTC_881|Compound:0833-0569|FD-Score:4.78|P-value:4.65E-7 ID:SGTC_921|Compound:1561-0023|FD-Score:3.34|P-value:2.69E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:4.05|P-value:1.49E-5 ID:SGTC_998|Compound:3996-0085|FD-Score:2.98|P-value:9.79E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.19|P-value:4.79E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:4.55|P-value:1.48E-6 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.42|P-value:2.04E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.49|P-value:1.57E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.19|P-value:4.77E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.84|P-value:3.41E-7 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.52|P-value:3.02E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.11|P-value:6.18E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.05|P-value:7.85E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:6.72|P-value:3.64E-12 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.72|P-value:1.41E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:7.66|P-value:3.07E-15 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.02|P-value:8.44E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-4.28|P-value:1.35E-5 ID:SGTC_1755|Compound:st041960|FD-Score:-3.27|P-value:7.26E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.62|P-value:9.22E-5 ID:SGTC_1839|Compound:st053978|FD-Score:3.43|P-value:1.93E-4 ID:SGTC_1849|Compound:st056239|FD-Score:4.53|P-value:1.60E-6 ID:SGTC_1853|Compound:st056710|FD-Score:3.49|P-value:1.55E-4 ID:SGTC_1859|Compound:st055998|FD-Score:-3.33|P-value:5.96E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.27|P-value:3.54E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.24|P-value:3.99E-4 ID:SGTC_2060|Compound:5238662|FD-Score:4.14|P-value:1.01E-5 ID:SGTC_2191|Compound:5911152|FD-Score:3.18|P-value:4.85E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.03|P-value:8.18E-4 ID:SGTC_2313|Compound:7726885|FD-Score:6.52|P-value:1.44E-11 ID:SGTC_2353|Compound:9059630|FD-Score:3.80|P-value:4.43E-5 ID:SGTC_2363|Compound:9053361|FD-Score:3.15|P-value:5.36E-4 ID:SGTC_2390|Compound:9097792|FD-Score:6.92|P-value:8.66E-13 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.92|P-value:2.73E-5 ID:SGTC_2585|Compound:alliin|FD-Score:3.13|P-value:5.92E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:4.45|P-value:2.44E-6 ID:SGTC_2602|Compound:kawain|FD-Score:4.01|P-value:1.82E-5 ID:SGTC_2860|Compound:9025725|FD-Score:-4.70|P-value:1.96E-6 ID:SGTC_2906|Compound:7926292|FD-Score:-3.78|P-value:1.09E-4 ID:SGTC_2919|Compound:7978188|FD-Score:-3.57|P-value:2.48E-4 ID:SGTC_2924|Compound:9010168|FD-Score:3.86|P-value:3.41E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-5.43|P-value:4.28E-8 ID:SGTC_2975|Compound:9084847|FD-Score:4.49|P-value:1.99E-6 ID:SGTC_2986|Compound:9022445|FD-Score:-3.91|P-value:6.54E-5 ID:SGTC_3027|Compound:9089541|FD-Score:3.11|P-value:6.20E-4 ID:SGTC_3076|Compound:9116187|FD-Score:4.55|P-value:1.51E-6 ID:SGTC_3126|Compound:9127922|FD-Score:5.92|P-value:7.30E-10 ID:SGTC_3131|Compound:9127911|FD-Score:-4.23|P-value:1.66E-5 ID:SGTC_3135|Compound:9127742|FD-Score:4.80|P-value:4.30E-7 ID:SGTC_3214|Compound:9129694|FD-Score:3.29|P-value:3.27E-4 ID:SGTC_3242|Compound:9133940|FD-Score:-3.23|P-value:8.43E-4 ID:SGTC_118|Compound:0083-0097|FD-Score:6.26|P-value:8.51E-11 ID:SGTC_123|Compound:0083-0120|FD-Score:4.61|P-value:1.08E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:4.11|P-value:1.15E-5 ID:SGTC_188|Compound:3229-0966|FD-Score:3.15|P-value:5.43E-4 ID:SGTC_253|Compound:dyclonine|FD-Score:4.03|P-value:1.67E-5 ID:SGTC_269|Compound:exalamide|FD-Score:3.83|P-value:3.96E-5 ID:SGTC_276|Compound:colchicine|FD-Score:-4.39|P-value:8.11E-6 ID:SGTC_310|Compound:1273-0058|FD-Score:-7.17|P-value:6.12E-13 ID:SGTC_581|Compound:0958-0271|FD-Score:4.01|P-value:1.80E-5 ID:SGTC_625|Compound:k213-0058|FD-Score:4.42|P-value:2.84E-6 ID:SGTC_628|Compound:1636-0253|FD-Score:-3.60|P-value:2.22E-4 ID:SGTC_708|Compound:3448-5400|FD-Score:3.42|P-value:2.01E-4 ID:SGTC_717|Compound:1532-0246|FD-Score:5.27|P-value:3.42E-8 ID:SGTC_831|Compound:k061-1017|FD-Score:5.42|P-value:1.42E-8 ID:SGTC_832|Compound:4083-0019|FD-Score:5.72|P-value:2.46E-9 ID:SGTC_881|Compound:0833-0569|FD-Score:4.78|P-value:4.65E-7 ID:SGTC_921|Compound:1561-0023|FD-Score:3.34|P-value:2.69E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:4.05|P-value:1.49E-5 ID:SGTC_998|Compound:3996-0085|FD-Score:2.98|P-value:9.79E-4 ID:SGTC_1013|Compound:4112-3890|FD-Score:3.19|P-value:4.79E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:4.55|P-value:1.48E-6 ID:SGTC_1140|Compound:3229-1848|FD-Score:3.42|P-value:2.04E-4 ID:SGTC_1150|Compound:3909-7983|FD-Score:3.49|P-value:1.57E-4 ID:SGTC_1184|Compound:1269-1718|FD-Score:3.19|P-value:4.77E-4 ID:SGTC_1190|Compound:1486-1332|FD-Score:4.84|P-value:3.41E-7 ID:SGTC_1203|Compound:3992-3522|FD-Score:-3.52|P-value:3.02E-4 ID:SGTC_1371|Compound:2421-0008|FD-Score:3.11|P-value:6.18E-4 ID:SGTC_1395|Compound:3486-0190|FD-Score:3.05|P-value:7.85E-4 ID:SGTC_1416|Compound:3966-0321|FD-Score:6.72|P-value:3.64E-12 ID:SGTC_1527|Compound:coumarin|FD-Score:-3.72|P-value:1.41E-4 ID:SGTC_1529|Compound:apigenin|FD-Score:7.66|P-value:3.07E-15 ID:SGTC_1583|Compound:clindamycin|FD-Score:3.02|P-value:8.44E-4 ID:SGTC_1678|Compound:st019265|FD-Score:-4.28|P-value:1.35E-5 ID:SGTC_1755|Compound:st041960|FD-Score:-3.27|P-value:7.26E-4 ID:SGTC_1768|Compound:st044301|FD-Score:3.62|P-value:9.22E-5 ID:SGTC_1839|Compound:st053978|FD-Score:3.43|P-value:1.93E-4 ID:SGTC_1849|Compound:st056239|FD-Score:4.53|P-value:1.60E-6 ID:SGTC_1853|Compound:st056710|FD-Score:3.49|P-value:1.55E-4 ID:SGTC_1859|Compound:st055998|FD-Score:-3.33|P-value:5.96E-4 ID:SGTC_1912|Compound:st060209|FD-Score:3.27|P-value:3.54E-4 ID:SGTC_2056|Compound:5235370|FD-Score:3.24|P-value:3.99E-4 ID:SGTC_2060|Compound:5238662|FD-Score:4.14|P-value:1.01E-5 ID:SGTC_2191|Compound:5911152|FD-Score:3.18|P-value:4.85E-4 ID:SGTC_2304|Compound:7443016|FD-Score:3.03|P-value:8.18E-4 ID:SGTC_2313|Compound:7726885|FD-Score:6.52|P-value:1.44E-11 ID:SGTC_2353|Compound:9059630|FD-Score:3.80|P-value:4.43E-5 ID:SGTC_2363|Compound:9053361|FD-Score:3.15|P-value:5.36E-4 ID:SGTC_2390|Compound:9097792|FD-Score:6.92|P-value:8.66E-13 ID:SGTC_2489|Compound:cantharidin|FD-Score:3.92|P-value:2.73E-5 ID:SGTC_2585|Compound:alliin|FD-Score:3.13|P-value:5.92E-4 ID:SGTC_2594|Compound:a-mangostin|FD-Score:4.45|P-value:2.44E-6 ID:SGTC_2602|Compound:kawain|FD-Score:4.01|P-value:1.82E-5 ID:SGTC_2860|Compound:9025725|FD-Score:-4.70|P-value:1.96E-6 ID:SGTC_2906|Compound:7926292|FD-Score:-3.78|P-value:1.09E-4 ID:SGTC_2919|Compound:7978188|FD-Score:-3.57|P-value:2.48E-4 ID:SGTC_2924|Compound:9010168|FD-Score:3.86|P-value:3.41E-5 ID:SGTC_2928|Compound:9011484|FD-Score:-5.43|P-value:4.28E-8 ID:SGTC_2975|Compound:9084847|FD-Score:4.49|P-value:1.99E-6 ID:SGTC_2986|Compound:9022445|FD-Score:-3.91|P-value:6.54E-5 ID:SGTC_3027|Compound:9089541|FD-Score:3.11|P-value:6.20E-4 ID:SGTC_3076|Compound:9116187|FD-Score:4.55|P-value:1.51E-6 ID:SGTC_3126|Compound:9127922|FD-Score:5.92|P-value:7.30E-10 ID:SGTC_3131|Compound:9127911|FD-Score:-4.23|P-value:1.66E-5 ID:SGTC_3135|Compound:9127742|FD-Score:4.80|P-value:4.30E-7 ID:SGTC_3214|Compound:9129694|FD-Score:3.29|P-value:3.27E-4 ID:SGTC_3242|Compound:9133940|FD-Score:-3.23|P-value:8.43E-4

Top fitness defect scores for YHR010W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1529 apigenin 7.66 74.0 µM 3.07E-15 3 9.00E-15
2 SGTC_2390 9097792 6.92 176.6 µM 8.66E-13 1 2.24E-12
3 SGTC_1416 3966-0321 6.72 25.3 µM 3.64E-12 2 9.10E-12
4 SGTC_2313 7726885 6.52 200.0 µM 1.44E-11 RPP1 & pyrimidine depletion 2 3.50E-11
5 SGTC_118 0083-0097 6.26 135.3 µM 8.51E-11 translation 16 1.98E-10
6 SGTC_3126 9127922 5.92 49.5 µM 7.30E-10 fatty acid desaturase (OLE1) 16 1.61E-9
7 SGTC_832 4083-0019 5.72 164.0 µM 2.46E-9 translation 25 5.27E-9
8 SGTC_831 k061-1017 5.42 217.0 µM 1.42E-8 translation 15 2.91E-8
9 SGTC_717 1532-0246 5.27 73.2 µM 3.42E-8 Golgi 8 6.85E-8
10 SGTC_1190 1486-1332 4.84 13.0 µM 3.41E-7 3 6.42E-7
11 SGTC_3135 9127742 4.80 49.5 µM 4.30E-7 8 8.04E-7
12 SGTC_881 0833-0569 4.78 170.0 µM 4.65E-7 8 8.67E-7
13 SGTC_123 0083-0120 4.61 66.7 µM 1.08E-6 31 1.97E-6
14 SGTC_1032 k061-1018 4.55 98.7 µM 1.48E-6 9 2.67E-6
15 SGTC_3076 9116187 4.55 49.5 µM 1.51E-6 11 2.72E-6
16 SGTC_1849 st056239 4.53 19.2 µM 1.60E-6 RPP1 & pyrimidine depletion 13 2.89E-6
17 SGTC_2975 9084847 4.49 53.7 µM 1.99E-6 13 3.57E-6
18 SGTC_2594 a-mangostin 4.45 100.0 µM 2.44E-6 19 4.34E-6
19 SGTC_625 k213-0058 4.42 121.0 µM 2.84E-6 translation 75 5.04E-6
20 SGTC_2060 5238662 4.14 140.0 µM 1.01E-5 23 1.72E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.151 1.60E-18 YDR140W MTQ2 hom S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; subunit of complex with Trm112p that methylates translation release factor Sup45p (eRF1) in the ternary complex eRF1-eRF3-GTP; similar to E.coli PrmC
0.149 4.50E-18 YPL125W KAP120 hom Karyopherin responsible for the nuclear import of ribosome maturation factor Rfp1p
0.146 2.03E-17 YBR017C KAP104 hom Transportin or cytosolic karyopherin beta 2; functions in the rg-nuclear localization signal-mediated nuclear import/reimport of mRNA-binding proteins Nab2p and Hrp1p; regulates asymmetric protein synthesis in daughter cells during mitosis
0.143 9.62E-17 YBR191W RPL21A hom Ribosomal 60S subunit protein L21A; homologous to mammalian ribosomal protein L21, no bacterial homolog; RPL21A has a paralog, RPL21B, that arose from the whole genome duplication
0.142 1.12E-16 YLR058C SHM2 hom Cytosolic serine hydroxymethyltransferase, converts serine to glycine plus 5,10 methylenetetrahydrofolate; major isoform involved in generating precursors for purine, pyrimidine, amino acid, and lipid biosynthesis
0.142 1.62E-16 YLR048W RPS0B hom Protein component of the small (40S) ribosomal subunit; RPS0B has a paralog, RPS0A, that arose from the whole genome duplication; required for maturation of 18S rRNA along with Rps0Ap; deletion of either RPS0 gene reduces growth rate, deletion of both genes is lethal; homologous to human ribosomal protein SA and bacterial S2
0.120 3.65E-12 YDL081C RPP1A hom Ribosomal stalk protein P1 alpha, involved in the interaction between translational elongation factors and the ribosome; accumulation of P1 in the cytoplasm is regulated by phosphorylation and interaction with the P2 stalk component
0.116 1.86E-11 YGR162W TIF4631 hom Translation initiation factor eIF4G; subunit of the mRNA cap-binding protein complex (eIF4F) that also contains eIF4E (Cdc33p); interacts with Pab1p and with eIF4A (Tif1p); also has a role in biogenesis of the large ribosomal subunit; TIF4631 has a paralog, TIF4632, that arose from the whole genome duplication
0.115 2.69E-11 YOR085W OST3 hom Gamma subunit of the oligosaccharyltransferase complex of the ER lumen, which catalyzes asparagine-linked glycosylation of newly synthesized proteins; Ost3p is important for N-glycosylation of a subset of proteins
0.114 3.44E-11 YJL188C_d BUD19_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 88% of ORF overlaps the verified gene RPL39; diploid mutant displays a weak budding pattern phenotype in a systematic assay
0.110 1.72E-10 YOR073W SGO1 hom Component of the spindle checkpoint, involved in sensing lack of tension on mitotic chromosomes; protects centromeric Rec8p at meiosis I; required for accurate chromosomal segregation at meiosis II and for mitotic chromosome stability
0.108 2.99E-10 YDR174W HMO1 hom Chromatin associated high mobility group (HMG) family member involved in genome maintenance; rDNA-binding component of the Pol I transcription system; associates with a 5'-3' DNA helicase and Fpr1p, a prolyl isomerase
0.108 3.02E-10 YNL227C JJJ1 hom Co-chaperone that stimulates the ATPase activity of Ssa1p, required for a late step of ribosome biogenesis; associated with the cytosolic large ribosomal subunit; contains a J-domain; mutation causes defects in fluid-phase endocytosis
0.107 5.71E-10 YDL075W RPL31A hom Ribosomal 60S subunit protein L31A; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31A has a paralog, RPL31B, that arose from the whole genome duplication
0.105 1.19E-9 YLR275W SMD2 het Core Sm protein Sm D2; part of heteroheptameric complex (with Smb1p, Smd1p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D2