YHR018C / ARG4

Argininosuccinate lyase, catalyzes the final step in the arginine biosynthesis pathway

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR018C / ARG4

Click on Significant Values for Screen Details ID:SGTC_42|Compound:0133-0086|FD-Score:4.41|P-value:1.68E-6 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.60|P-value:7.67E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:9.47|P-value:9.53E-24 ID:SGTC_58|Compound:0957-0393|FD-Score:3.44|P-value:1.49E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:3.69|P-value:5.10E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.12|P-value:4.67E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.18|P-value:4.20E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:5.47|P-value:4.08E-9 ID:SGTC_740|Compound:k014-0057|FD-Score:-4.49|P-value:1.01E-6 ID:SGTC_752|Compound:1896-0090|FD-Score:-3.16|P-value:4.02E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:-2.92|P-value:9.91E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.07|P-value:9.25E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.55|P-value:9.46E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.00|P-value:8.16E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.12|P-value:5.21E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-4.67|P-value:3.81E-7 ID:SGTC_1750|Compound:st081859|FD-Score:2.99|P-value:8.33E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.39|P-value:1.85E-4 ID:SGTC_1917|Compound:st066507|FD-Score:4.17|P-value:5.72E-6 ID:SGTC_1942|Compound:st076330|FD-Score:5.00|P-value:6.99E-8 ID:SGTC_2015|Compound:5119600|FD-Score:5.08|P-value:4.30E-8 ID:SGTC_2070|Compound:5230947|FD-Score:-3.01|P-value:7.24E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.80|P-value:3.16E-5 ID:SGTC_2362|Compound:9071156|FD-Score:4.17|P-value:5.60E-6 ID:SGTC_2425|Compound:5628681|FD-Score:3.58|P-value:8.30E-5 ID:SGTC_2451|Compound:5856223|FD-Score:-2.93|P-value:9.63E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:4.73|P-value:3.14E-7 ID:SGTC_2691|Compound:st077356|FD-Score:3.17|P-value:4.34E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.00|P-value:8.06E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.56|P-value:9.20E-5 ID:SGTC_2813|Compound:7971799|FD-Score:4.45|P-value:1.42E-6 ID:SGTC_42|Compound:0133-0086|FD-Score:4.41|P-value:1.68E-6 ID:SGTC_46|Compound:0329-0187a|FD-Score:3.60|P-value:7.67E-5 ID:SGTC_54|Compound:0159-0055|FD-Score:9.47|P-value:9.53E-24 ID:SGTC_58|Compound:0957-0393|FD-Score:3.44|P-value:1.49E-4 ID:SGTC_135|Compound:0933-0004|FD-Score:3.69|P-value:5.10E-5 ID:SGTC_136|Compound:0097-0015|FD-Score:-3.12|P-value:4.67E-4 ID:SGTC_283|Compound:0987-0079|FD-Score:3.18|P-value:4.20E-4 ID:SGTC_738|Compound:1315-0003|FD-Score:5.47|P-value:4.08E-9 ID:SGTC_740|Compound:k014-0057|FD-Score:-4.49|P-value:1.01E-6 ID:SGTC_752|Compound:1896-0090|FD-Score:-3.16|P-value:4.02E-4 ID:SGTC_1394|Compound:3474-0001|FD-Score:-2.92|P-value:9.91E-4 ID:SGTC_1456|Compound:k015-0012|FD-Score:4.07|P-value:9.25E-6 ID:SGTC_1506|Compound:4226-2149|FD-Score:3.55|P-value:9.46E-5 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.00|P-value:8.16E-4 ID:SGTC_1571|Compound:st070080|FD-Score:3.12|P-value:5.21E-4 ID:SGTC_1723|Compound:st034307|FD-Score:-4.67|P-value:3.81E-7 ID:SGTC_1750|Compound:st081859|FD-Score:2.99|P-value:8.33E-4 ID:SGTC_1885|Compound:st057546|FD-Score:3.39|P-value:1.85E-4 ID:SGTC_1917|Compound:st066507|FD-Score:4.17|P-value:5.72E-6 ID:SGTC_1942|Compound:st076330|FD-Score:5.00|P-value:6.99E-8 ID:SGTC_2015|Compound:5119600|FD-Score:5.08|P-value:4.30E-8 ID:SGTC_2070|Compound:5230947|FD-Score:-3.01|P-value:7.24E-4 ID:SGTC_2288|Compound:7946289|FD-Score:3.80|P-value:3.16E-5 ID:SGTC_2362|Compound:9071156|FD-Score:4.17|P-value:5.60E-6 ID:SGTC_2425|Compound:5628681|FD-Score:3.58|P-value:8.30E-5 ID:SGTC_2451|Compound:5856223|FD-Score:-2.93|P-value:9.63E-4 ID:SGTC_2518|Compound:prenyletin|FD-Score:4.73|P-value:3.14E-7 ID:SGTC_2691|Compound:st077356|FD-Score:3.17|P-value:4.34E-4 ID:SGTC_2692|Compound:st077426|FD-Score:3.00|P-value:8.06E-4 ID:SGTC_2693|Compound:st077581|FD-Score:3.56|P-value:9.20E-5 ID:SGTC_2813|Compound:7971799|FD-Score:4.45|P-value:1.42E-6

Top fitness defect scores for YHR018C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_54 0159-0055 9.47 9.0 µM 9.53E-24 ERG2 10 1.46E-21
2 SGTC_738 1315-0003 5.47 72.4 µM 4.08E-9 4 2.20E-8
3 SGTC_2015 5119600 5.08 184.0 µM 4.30E-8 13 1.84E-7
4 SGTC_1942 st076330 5.00 65.2 µM 6.99E-8 10 2.86E-7
5 SGTC_2518 prenyletin 4.73 46.3 µM 3.14E-7 5 1.11E-6
6 SGTC_2813 7971799 4.45 71.4 µM 1.42E-6 13 4.32E-6
7 SGTC_42 0133-0086 4.41 60.6 µM 1.68E-6 17 5.05E-6
8 SGTC_2362 9071156 4.17 126.0 µM 5.60E-6 5 1.50E-5
9 SGTC_1917 st066507 4.17 28.5 µM 5.72E-6 Golgi 30 1.53E-5
10 SGTC_1456 k015-0012 4.07 13.0 µM 9.25E-6 30 2.36E-5
11 SGTC_2288 7946289 3.80 167.3 µM 3.16E-5 32 7.17E-5
12 SGTC_135 0933-0004 3.69 26.4 µM 5.10E-5 60S ribosome export 30 1.11E-4
13 SGTC_46 0329-0187a 3.60 2.2 µM 7.67E-5 91 1.60E-4
14 SGTC_2425 5628681 3.58 7.8 µM 8.30E-5 37 1.72E-4
15 SGTC_2693 st077581 3.56 40.7 µM 9.20E-5 51 1.89E-4
16 SGTC_1506 4226-2149 3.55 212.0 µM 9.46E-5 44 1.94E-4
17 SGTC_58 0957-0393 3.44 9.0 µM 1.49E-4 cell wall 434 2.92E-4
18 SGTC_1885 st057546 3.39 99.9 µM 1.85E-4 32 3.56E-4
19 SGTC_283 0987-0079 3.18 11.7 µM 4.20E-4 59 7.47E-4
20 SGTC_2691 st077356 3.17 60.2 µM 4.34E-4 67 7.71E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.198 4.51E-31 YOL058W ARG1 hom Arginosuccinate synthetase, catalyzes the formation of L-argininosuccinate from citrulline and L-aspartate in the arginine biosynthesis pathway; potential Cdc28p substrate
0.192 3.48E-29 YOL140W ARG8 hom Acetylornithine aminotransferase, catalyzes the fourth step in the biosynthesis of the arginine precursor ornithine
0.140 4.41E-16 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.137 1.60E-15 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.131 2.11E-14 YOR303W CPA1 hom Small subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor; translationally regulated by an attenuator peptide encoded by YOR302W within the CPA1 mRNA 5'-leader
0.120 3.59E-12 YOR130C ORT1 hom Ornithine transporter of the mitochondrial inner membrane, exports ornithine from mitochondria as part of arginine biosynthesis; human ortholog is associated with hyperammonaemia-hyperornithinaemia-homocitrullinuria (HHH) syndrome
0.119 4.08E-12 YJL088W ARG3 hom Ornithine carbamoyltransferase (carbamoylphosphate:L-ornithine carbamoyltransferase), catalyzes the sixth step in the biosynthesis of the arginine precursor ornithine
0.109 2.52E-10 YDL189W RBS1 hom Protein of unknown function, identified as a high copy suppressor of psk1 psk2 mutations that confer temperature-sensitivity for galactose utilization; proposed to bind single-stranded nucleic acids via its R3H domain
0.108 3.58E-10 YML057C-A_d YML057C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene CMP2/YML057W
0.107 5.88E-10 YJR109C CPA2 hom Large subunit of carbamoyl phosphate synthetase, which catalyzes a step in the synthesis of citrulline, an arginine precursor
0.105 1.06E-9 YBR120C CBP6 hom Mitochondrial protein required for translation of the COB mRNA; forms a complex with Cbp3p that binds to mt ribosomes near the polypeptide tunnel exit and promotes efficient translation of the COB mRNA; Cbp3p-Cbp6p complex also interacts with newly synthesized cytochrome b (Cobp) and Cbp4p to promote assembly of Cobp into the cytochrome bc1 complex
0.103 2.49E-9 YJL071W ARG2 hom Acetylglutamate synthase (glutamate N-acetyltransferase), mitochondrial enzyme that catalyzes the first step in the biosynthesis of the arginine precursor ornithine; forms a complex with Arg5,6p
0.101 5.40E-9 YMR133W REC114 hom Protein involved in early stages of meiotic recombination; possibly involved in the coordination of recombination and meiotic division; mutations lead to premature initiation of the first meiotic division
0.097 1.79E-8 YJR054W KCH1 hom Potassium transporter that mediates K+ influx; activates the high-affinity Ca2+ influx system (HACS) during the mating pheromone response; expression is up-regulated in response to alpha factor; localized to sites of polarized growth; similar to Prm6p; member of a fungal-specific gene family; potential Cdc28p substrate
0.092 9.88E-8 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity