YHR019C / DED81

Cytosolic asparaginyl-tRNA synthetase, required for protein synthesis, catalyzes the specific attachment of asparagine to its cognate tRNA

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR019C / DED81

Click on Significant Values for Screen Details ID:SGTC_116|Compound:0155-0182|FD-Score:-10.40|P-value:2.51E-21 ID:SGTC_125|Compound:0133-0007|FD-Score:-3.78|P-value:3.50E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-4.69|P-value:1.19E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:4.25|P-value:3.86E-5 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:9.07|P-value:3.21E-17 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:4.52|P-value:1.34E-5 ID:SGTC_627|Compound:1391-0720|FD-Score:4.18|P-value:5.17E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:-7.10|P-value:6.80E-11 ID:SGTC_703|Compound:3875-0075|FD-Score:3.92|P-value:1.34E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.60|P-value:6.22E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.78|P-value:3.53E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.99|P-value:1.69E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.72|P-value:4.23E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.45|P-value:6.44E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:4.03|P-value:8.99E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.75|P-value:2.40E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:-4.12|P-value:1.07E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:6.29|P-value:3.07E-9 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.66|P-value:3.23E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.69|P-value:6.92E-6 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.71|P-value:2.72E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.53|P-value:7.95E-4 ID:SGTC_1866|Compound:st057638|FD-Score:-4.45|P-value:3.16E-5 ID:SGTC_1881|Compound:5652484|FD-Score:3.75|P-value:2.44E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-4.60|P-value:1.77E-5 ID:SGTC_2371|Compound:9071574|FD-Score:4.07|P-value:7.65E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:-4.50|P-value:2.58E-5 ID:SGTC_2850|Compound:9015429|FD-Score:3.61|P-value:3.82E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.64|P-value:3.45E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-5.04|P-value:2.81E-6 ID:SGTC_2922|Compound:7999257|FD-Score:5.09|P-value:1.23E-6 ID:SGTC_2923|Compound:7992654|FD-Score:3.61|P-value:3.83E-4 ID:SGTC_2924|Compound:9010168|FD-Score:5.27|P-value:5.23E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-3.55|P-value:7.27E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.62|P-value:3.73E-4 ID:SGTC_3092|Compound:9117248|FD-Score:4.34|P-value:2.80E-5 ID:SGTC_3099|Compound:9120367|FD-Score:3.50|P-value:5.55E-4 ID:SGTC_3307|Compound:9126885|FD-Score:4.45|P-value:1.80E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:-10.40|P-value:2.51E-21 ID:SGTC_125|Compound:0133-0007|FD-Score:-3.78|P-value:3.50E-4 ID:SGTC_126|Compound:0417-1624|FD-Score:-4.69|P-value:1.19E-5 ID:SGTC_425|Compound:3825-7017|FD-Score:4.25|P-value:3.86E-5 ID:SGTC_436|Compound:n,n-dimethylsphingosine|FD-Score:9.07|P-value:3.21E-17 ID:SGTC_474|Compound:dl-dihydrosphingosine|FD-Score:4.52|P-value:1.34E-5 ID:SGTC_627|Compound:1391-0720|FD-Score:4.18|P-value:5.17E-5 ID:SGTC_691|Compound:1548-0686|FD-Score:-7.10|P-value:6.80E-11 ID:SGTC_703|Compound:3875-0075|FD-Score:3.92|P-value:1.34E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:-3.60|P-value:6.22E-4 ID:SGTC_711|Compound:k292-0785|FD-Score:-3.78|P-value:3.53E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-3.99|P-value:1.69E-4 ID:SGTC_925|Compound:2064-1272|FD-Score:-3.72|P-value:4.23E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.45|P-value:6.44E-4 ID:SGTC_1020|Compound:4335-0961|FD-Score:4.03|P-value:8.99E-5 ID:SGTC_1023|Compound:4482-3888|FD-Score:3.75|P-value:2.40E-4 ID:SGTC_1169|Compound:aniline yellow|FD-Score:-4.12|P-value:1.07E-4 ID:SGTC_1170|Compound:0310-0127|FD-Score:6.29|P-value:3.07E-9 ID:SGTC_1175|Compound:0741-0056|FD-Score:3.66|P-value:3.23E-4 ID:SGTC_1179|Compound:1021-1431|FD-Score:4.69|P-value:6.92E-6 ID:SGTC_1212|Compound:0520-0165|FD-Score:3.71|P-value:2.72E-4 ID:SGTC_1303|Compound:1160-0052|FD-Score:-3.53|P-value:7.95E-4 ID:SGTC_1866|Compound:st057638|FD-Score:-4.45|P-value:3.16E-5 ID:SGTC_1881|Compound:5652484|FD-Score:3.75|P-value:2.44E-4 ID:SGTC_2309|Compound:7664088|FD-Score:-4.60|P-value:1.77E-5 ID:SGTC_2371|Compound:9071574|FD-Score:4.07|P-value:7.65E-5 ID:SGTC_2555|Compound:cholic acid|FD-Score:-4.50|P-value:2.58E-5 ID:SGTC_2850|Compound:9015429|FD-Score:3.61|P-value:3.82E-4 ID:SGTC_2857|Compound:9024691|FD-Score:3.64|P-value:3.45E-4 ID:SGTC_2859|Compound:9028643|FD-Score:-5.04|P-value:2.81E-6 ID:SGTC_2922|Compound:7999257|FD-Score:5.09|P-value:1.23E-6 ID:SGTC_2923|Compound:7992654|FD-Score:3.61|P-value:3.83E-4 ID:SGTC_2924|Compound:9010168|FD-Score:5.27|P-value:5.23E-7 ID:SGTC_2965|Compound:9082772|FD-Score:-3.55|P-value:7.27E-4 ID:SGTC_2969|Compound:9091408|FD-Score:3.62|P-value:3.73E-4 ID:SGTC_3092|Compound:9117248|FD-Score:4.34|P-value:2.80E-5 ID:SGTC_3099|Compound:9120367|FD-Score:3.50|P-value:5.55E-4 ID:SGTC_3307|Compound:9126885|FD-Score:4.45|P-value:1.80E-5

Top fitness defect scores for YHR019C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_436 n,n-dimethylsphingosine 9.07 3.3 µM 3.21E-17 1 5.74E-20 5.30
2 SGTC_1170 0310-0127 6.29 64.8 µM 3.07E-9 1 1.59E-10 0.75
3 SGTC_2924 9010168 5.27 62.8 µM 5.23E-7 cell wall signaling 1 6.75E-8 0.57
4 SGTC_2922 7999257 5.09 32.5 µM 1.23E-6 3 1.83E-7 0.69
5 SGTC_1179 1021-1431 4.69 9.3 µM 6.92E-6 3 1.40E-6 0.01
6 SGTC_474 dl-dihydrosphingosine 4.52 5.0 µM 1.34E-5 4 3.03E-6 0.72
7 SGTC_3307 9126885 4.45 69.3 µM 1.80E-5 6 4.28E-6 0.29
8 SGTC_3092 9117248 4.34 49.5 µM 2.80E-5 cell wall signaling 1 7.17E-6 0.95
9 SGTC_425 3825-7017 4.25 13.1 µM 3.86E-5 6 1.05E-5 0.02
10 SGTC_627 1391-0720 4.18 175.0 µM 5.17E-5 2 1.47E-5 0.33
11 SGTC_2371 9071574 4.07 200.0 µM 7.65E-5 1 2.33E-5 0.35
12 SGTC_1020 4335-0961 4.03 93.3 µM 8.99E-5 6 2.81E-5 0.18
13 SGTC_703 3875-0075 3.92 71.8 µM 1.34E-4 5 4.49E-5 0.07
14 SGTC_1023 4482-3888 3.75 16.0 µM 2.40E-4 TSC3-RPN4 10 8.85E-5 0.03
15 SGTC_1881 5652484 3.75 16.0 µM 2.44E-4 TRP & mitochondrial translation 7 9.02E-5 0.02
16 SGTC_1212 0520-0165 3.71 585.0 µM 2.72E-4 7 1.02E-4 0.37
17 SGTC_1175 0741-0056 3.66 27.6 µM 3.23E-4 7 1.25E-4 0.05
18 SGTC_2857 9024691 3.64 58.4 µM 3.45E-4 5 1.35E-4 0.23
19 SGTC_2969 9091408 3.62 28.7 µM 3.73E-4 11 1.48E-4 0.03
20 SGTC_2850 9015429 3.61 58.4 µM 3.82E-4 7 1.52E-4 0.08

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.145 3.27E-17 YDL007W RPT2 het One of six ATPases of the 19S regulatory particle of the 26S proteasome involved in the degradation of ubiquitinated substrates; required for normal peptide hydrolysis by the core 20S particle
0.143 6.81E-17 YJL174W KRE9 het Glycoprotein involved in cell wall beta-glucan assembly; null mutation leads to severe growth defects, aberrant multibudded morphology, and mating defects
0.143 8.25E-17 YOR159C SME1 het Core Sm protein Sm E; part of heteroheptameric complex (with Smb1p, Smd1p, Smd2p, Smd3p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm E
0.142 1.56E-16 YLR195C NMT1 het N-myristoyl transferase, catalyzes the cotranslational, covalent attachment of myristic acid to the N-terminal glycine residue of several proteins involved in cellular growth and signal transduction
0.136 2.95E-15 YLR096W KIN2 hom Serine/threonine protein kinase involved in regulation of exocytosis; localizes to the cytoplasmic face of the plasma membrane; closely related to Kin1p
0.133 1.31E-14 YDR235W PRP42 het U1 snRNP protein involved in splicing, required for U1 snRNP biogenesis; contains multiple tetriatricopeptide repeats
0.131 3.20E-14 YER043C SAH1 het S-adenosyl-L-homocysteine hydrolase, catabolizes S-adenosyl-L-homocysteine which is formed after donation of the activated methyl group of S-adenosyl-L-methionine (AdoMet) to an acceptor
0.127 1.95E-13 YLR026C SED5 het cis-Golgi t-SNARE syntaxin required for vesicular transport between the ER and the Golgi complex, binds at least 9 SNARE proteins
0.125 4.77E-13 YDL209C CWC2 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; binds directly to U6 snRNA; similar to S. pombe Cwf2
0.121 2.25E-12 YDR021W FAL1 het Nucleolar protein required for maturation of 18S rRNA, member of the eIF4A subfamily of DEAD-box ATP-dependent RNA helicases
0.119 3.98E-12 YMR194C-B CMC4 hom Protein that localizes to the mitochondrial intermembrane space via the Mia40p-Erv1p system; contains twin cysteine-x(9)-cysteine motifs
0.117 1.29E-11 YBR155W CNS1 het TPR-containing co-chaperone; binds both Hsp82p (Hsp90) and Ssa1p (Hsp70) and stimulates the ATPase activity of SSA1, ts mutants reduce Hsp82p function while over expression suppresses the phenotypes of an HSP82 ts allele and a cpr7 deletion
0.116 1.70E-11 YDR478W SNM1 het Subunit of RNase MRP, which cleaves pre-rRNA and has a role in cell cycle-regulated degradation of daughter cell-specific mRNAs; binds to the NME1 RNA subunit of RNase MRP
0.115 3.04E-11 YBL084C CDC27 het Subunit of the Anaphase-Promoting Complex/Cyclosome (APC/C), which is a ubiquitin-protein ligase required for degradation of anaphase inhibitors, including mitotic cyclins, during the metaphase/anaphase transition
0.112 8.90E-11 YNL163C RIA1 het Cytoplasmic GTPase/eEF2-like factor involved in ribosomal biogenesis; with Sdo1p, promotes release of Tif6p from 60S ribosomal subunits in the cytoplasm so that they can assemble with 40S subunits to generate mature ribosomes; required for quality control check of newly made large ribosomal subunits before they are released into the pool of translating ribosomes