YHR020W

Prolyl-tRNA synthetase; N-terminal domain shows weak homology to prokaryotic posttransfer editing domain, but does not possess posttransfer editing activity; may interact with ribosomes, based on co-purification experiments

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR020W / YHR020W

Click on Significant Values for Screen Details ID:SGTC_9|Compound:0092-0002|FD-Score:2.96|P-value:5.86E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:4.16|P-value:2.58E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:5.33|P-value:2.60E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:3.46|P-value:7.41E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:4.75|P-value:9.86E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:6.30|P-value:2.48E-12 ID:SGTC_205|Compound:1486-1330|FD-Score:2.92|P-value:6.80E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:-2.87|P-value:8.30E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:-2.88|P-value:7.89E-4 ID:SGTC_325|Compound:2810-4341|FD-Score:3.07|P-value:3.80E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:2.84|P-value:9.36E-4 ID:SGTC_458|Compound:nppb|FD-Score:2.90|P-value:7.38E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.72|P-value:2.32E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:-2.84|P-value:9.13E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:-3.04|P-value:4.28E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.13|P-value:3.01E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:2.83|P-value:9.64E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:7.23|P-value:1.11E-15 ID:SGTC_852|Compound:0418-0143|FD-Score:2.93|P-value:6.65E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.81|P-value:1.50E-5 ID:SGTC_1029|Compound:k015-0030|FD-Score:2.84|P-value:9.25E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.02|P-value:4.70E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:3.19|P-value:2.36E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:4.36|P-value:8.89E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:-2.99|P-value:5.18E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-4.49|P-value:4.23E-7 ID:SGTC_1337|Compound:1486-1011|FD-Score:-3.01|P-value:4.73E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:-3.08|P-value:3.65E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.15|P-value:2.78E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-2.89|P-value:7.48E-4 ID:SGTC_1786|Compound:berberine|FD-Score:2.84|P-value:9.21E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.38|P-value:1.06E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-3.12|P-value:3.05E-4 ID:SGTC_2016|Compound:5121758|FD-Score:5.87|P-value:6.06E-11 ID:SGTC_2019|Compound:4016004|FD-Score:-2.94|P-value:6.22E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.06|P-value:4.07E-4 ID:SGTC_2191|Compound:5911152|FD-Score:2.86|P-value:8.64E-4 ID:SGTC_2265|Compound:7929201|FD-Score:-3.24|P-value:1.91E-4 ID:SGTC_2266|Compound:7929956|FD-Score:-2.84|P-value:9.31E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-3.50|P-value:6.08E-5 ID:SGTC_2379|Compound:9035213|FD-Score:4.16|P-value:2.59E-6 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.22|P-value:2.02E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:-4.25|P-value:1.52E-6 ID:SGTC_2511|Compound:berberine|FD-Score:6.31|P-value:2.28E-12 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.08|P-value:3.70E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.47|P-value:7.11E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.90|P-value:9.42E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:2.90|P-value:7.48E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.01|P-value:4.77E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.78|P-value:1.74E-5 ID:SGTC_3151|Compound:9099821|FD-Score:-2.87|P-value:8.22E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.96|P-value:7.09E-6 ID:SGTC_3298|Compound:9116858|FD-Score:3.54|P-value:5.16E-5 ID:SGTC_9|Compound:0092-0002|FD-Score:2.96|P-value:5.86E-4 ID:SGTC_113|Compound:0099-0221|FD-Score:4.16|P-value:2.58E-6 ID:SGTC_126|Compound:0417-1624|FD-Score:5.33|P-value:2.60E-9 ID:SGTC_143|Compound:4032-1194|FD-Score:3.46|P-value:7.41E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:4.75|P-value:9.86E-8 ID:SGTC_145|Compound:3737-0113|FD-Score:6.30|P-value:2.48E-12 ID:SGTC_205|Compound:1486-1330|FD-Score:2.92|P-value:6.80E-4 ID:SGTC_317|Compound:4495-0002|FD-Score:-2.87|P-value:8.30E-4 ID:SGTC_318|Compound:1488-0629|FD-Score:-2.88|P-value:7.89E-4 ID:SGTC_325|Compound:2810-4341|FD-Score:3.07|P-value:3.80E-4 ID:SGTC_370|Compound:0180-0345|FD-Score:2.84|P-value:9.36E-4 ID:SGTC_458|Compound:nppb|FD-Score:2.90|P-value:7.38E-4 ID:SGTC_522|Compound:1273-0010|FD-Score:3.72|P-value:2.32E-5 ID:SGTC_547|Compound:1120-0019|FD-Score:-2.84|P-value:9.13E-4 ID:SGTC_554|Compound:4483-2211|FD-Score:-3.04|P-value:4.28E-4 ID:SGTC_613|Compound:3914-0030|FD-Score:-3.13|P-value:3.01E-4 ID:SGTC_811|Compound:0416-0114|FD-Score:2.83|P-value:9.64E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:7.23|P-value:1.11E-15 ID:SGTC_852|Compound:0418-0143|FD-Score:2.93|P-value:6.65E-4 ID:SGTC_918|Compound:glabranin|FD-Score:3.81|P-value:1.50E-5 ID:SGTC_1029|Compound:k015-0030|FD-Score:2.84|P-value:9.25E-4 ID:SGTC_1040|Compound:k898-0338|FD-Score:3.02|P-value:4.70E-4 ID:SGTC_1057|Compound:3448-5381|FD-Score:3.19|P-value:2.36E-4 ID:SGTC_1269|Compound:0831-0796|FD-Score:4.36|P-value:8.89E-7 ID:SGTC_1313|Compound:1220-0047|FD-Score:-2.99|P-value:5.18E-4 ID:SGTC_1336|Compound:1486-0901|FD-Score:-4.49|P-value:4.23E-7 ID:SGTC_1337|Compound:1486-1011|FD-Score:-3.01|P-value:4.73E-4 ID:SGTC_1341|Compound:1486-1360|FD-Score:-3.08|P-value:3.65E-4 ID:SGTC_1578|Compound:josamycin|FD-Score:3.15|P-value:2.78E-4 ID:SGTC_1653|Compound:st012112|FD-Score:-2.89|P-value:7.48E-4 ID:SGTC_1786|Compound:berberine|FD-Score:2.84|P-value:9.21E-4 ID:SGTC_1910|Compound:st062352|FD-Score:-3.38|P-value:1.06E-4 ID:SGTC_1913|Compound:st060213|FD-Score:-3.12|P-value:3.05E-4 ID:SGTC_2016|Compound:5121758|FD-Score:5.87|P-value:6.06E-11 ID:SGTC_2019|Compound:4016004|FD-Score:-2.94|P-value:6.22E-4 ID:SGTC_2100|Compound:5377183|FD-Score:3.06|P-value:4.07E-4 ID:SGTC_2191|Compound:5911152|FD-Score:2.86|P-value:8.64E-4 ID:SGTC_2265|Compound:7929201|FD-Score:-3.24|P-value:1.91E-4 ID:SGTC_2266|Compound:7929956|FD-Score:-2.84|P-value:9.31E-4 ID:SGTC_2268|Compound:7933382|FD-Score:-3.50|P-value:6.08E-5 ID:SGTC_2379|Compound:9035213|FD-Score:4.16|P-value:2.59E-6 ID:SGTC_2508|Compound:celastrol|FD-Score:-3.22|P-value:2.02E-4 ID:SGTC_2510|Compound:benzyl isothiocyanate|FD-Score:-4.25|P-value:1.52E-6 ID:SGTC_2511|Compound:berberine|FD-Score:6.31|P-value:2.28E-12 ID:SGTC_2519|Compound:cryptotanshinone|FD-Score:-3.08|P-value:3.70E-4 ID:SGTC_2520|Compound:obtusaquinone|FD-Score:3.47|P-value:7.11E-5 ID:SGTC_2522|Compound:biochanin a|FD-Score:3.90|P-value:9.42E-6 ID:SGTC_2606|Compound:farnesol|FD-Score:2.90|P-value:7.48E-4 ID:SGTC_2630|Compound:antimycin a|FD-Score:3.01|P-value:4.77E-4 ID:SGTC_2640|Compound:pentamidine|FD-Score:3.78|P-value:1.74E-5 ID:SGTC_3151|Compound:9099821|FD-Score:-2.87|P-value:8.22E-4 ID:SGTC_3158|Compound:9100007|FD-Score:3.96|P-value:7.09E-6 ID:SGTC_3298|Compound:9116858|FD-Score:3.54|P-value:5.16E-5

Top fitness defect scores for YHR020W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_821 1114-0266 7.23 29.2 µM 1.11E-15 1 2.38E-13 2.42
2 SGTC_2511 berberine 6.31 10.5 µM 2.28E-12 60S ribosome export 4 1.38E-10 0.91
3 SGTC_145 3737-0113 6.30 38.4 µM 2.48E-12 DNA intercalators 15 1.48E-10 0.12
4 SGTC_2016 5121758 5.87 140.0 µM 6.06E-11 redox potentiating 6 2.14E-9 0.17
5 SGTC_126 0417-1624 5.33 672.5 µM 2.60E-9 DNA intercalators 6 4.96E-8 0.10
6 SGTC_144 4161-2749 4.75 112.4 µM 9.86E-8 DNA intercalators 8 1.04E-6 0.23
7 SGTC_1269 0831-0796 4.36 3.0 µM 8.89E-7 60S ribosome export 3 6.55E-6 0.11
8 SGTC_113 0099-0221 4.16 2.5 µM 2.58E-6 3 1.60E-5 0.36
9 SGTC_2379 9035213 4.16 200.0 µM 2.59E-6 4 1.61E-5 0.43
10 SGTC_3158 9100007 3.96 49.5 µM 7.09E-6 5 3.73E-5 0.10
11 SGTC_2522 biochanin a 3.90 4.9 µM 9.42E-6 RNA pol III & RNase P/MRP 12 4.74E-5 0.73
12 SGTC_918 glabranin 3.81 770.7 nM 1.50E-5 9 6.99E-5 0.14
13 SGTC_2640 pentamidine 3.78 10.4 µM 1.74E-5 mitochondrial processes 19 7.93E-5 0.01
14 SGTC_522 1273-0010 3.72 11.6 µM 2.32E-5 60S ribosome export 6 1.01E-4 0.08
15 SGTC_3298 9116858 3.54 71.4 µM 5.16E-5 6 1.98E-4 0.04
16 SGTC_2520 obtusaquinone 3.47 4.7 µM 7.11E-5 10 2.59E-4 0.10
17 SGTC_143 4032-1194 3.46 15.7 µM 7.41E-5 29 2.68E-4 0.02
18 SGTC_1057 3448-5381 3.19 134.0 µM 2.36E-4 calcium & mitochondrial duress 23 7.11E-4 0.00
19 SGTC_1578 josamycin 3.15 24.2 µM 2.78E-4 12 8.17E-4 0.05
20 SGTC_325 2810-4341 3.07 181.0 µM 3.80E-4 amide catabolism 14 0.00106 0.17

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.287 2.03E-64 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.236 9.98E-44 YDR054C CDC34 het Ubiquitin-conjugating enzyme (E2); catalytic subunit of SCF ubiquitin-protein ligase complex (together with Skp1p, Rbx1p, Cdc53p, and an F-box protein) that regulates cell cycle progression by targeting key substrates for degradation; protein abundance increases in response to DNA replication stress
0.203 1.59E-32 YHR149C SKG6 hom Integral membrane protein that localizes primarily to growing sites such as the bud tip or the cell periphery; potential Cdc28p substrate; Skg6p interacts with Zds1p and Zds2p
0.197 1.05E-30 YEL017C-A PMP2 hom Proteolipid associated with plasma membrane H(+)-ATPase (Pma1p); regulates plasma membrane H(+)-ATPase activity; protein abundance increases in response to DNA replication stress; PMP2 has a paralog, PMP1, that arose from the whole genome duplication
0.197 1.27E-30 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.195 4.37E-30 YEL064C AVT2 hom Putative transporter, member of a family of seven S. cerevisiae genes (AVT1-7) related to vesicular GABA-glycine transporters
0.189 1.96E-28 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.187 8.56E-28 YFL022C FRS2 het Alpha subunit of cytoplasmic phenylalanyl-tRNA synthetase, forms a tetramer with Frs1p to form active enzyme; evolutionarily distant from mitochondrial phenylalanyl-tRNA synthetase based on protein sequence, but substrate binding is similar
0.179 1.40E-25 YNL124W NAF1 het RNA-binding protein required for the assembly of box H/ACA snoRNPs and thus for pre-rRNA processing, forms a complex with Shq1p and interacts with H/ACA snoRNP components Nhp2p and Cbf5p; similar to Gar1p
0.173 6.71E-24 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.173 7.49E-24 YGL002W ERP6 hom Member of the p24 family involved in ER to Golgi transport; similar to Emp24p and Erv25p; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies; ERP6 has a paralog, ERP1, that arose from the whole genome duplication
0.159 1.96E-20 YOR077W RTS2 het Basic zinc-finger protein, similar to human and mouse Kin17 proteins which are chromatin-associated proteins involved in UV response and DNA replication
0.155 1.55E-19 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.151 1.45E-18 YMR030W RSF1 hom Protein required for respiratory growth; localized to both the nucleus and mitochondrion; may interact with transcription factors to mediate the transition to respiratory growth and activate transcription of nuclear and mitochondrial genes
0.150 3.08E-18 YNL264C PDR17 hom Phosphatidylinositol transfer protein (PITP), downregulates Plb1p-mediated turnover of phosphatidylcholine, found in the cytosol and microsomes, homologous to Pdr16p, deletion affects phospholipid composition