YHR021C / RPS27B

Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S27, no bacterial homolog; RPS27B has a paralog, RPS27A, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR021C / RPS27B

Click on Significant Values for Screen Details ID:SGTC_33|Compound:0986-0033|FD-Score:3.88|P-value:1.33E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.66|P-value:6.10E-6 ID:SGTC_276|Compound:colchicine|FD-Score:3.64|P-value:3.13E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.70|P-value:2.45E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.45|P-value:5.80E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.56|P-value:4.02E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-4.40|P-value:1.70E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.11|P-value:5.53E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.33|P-value:8.92E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.75|P-value:2.13E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:4.45|P-value:1.48E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:-3.43|P-value:6.14E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.30|P-value:9.67E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:6.94|P-value:3.41E-11 ID:SGTC_1430|Compound:4049-0248|FD-Score:-3.99|P-value:8.68E-5 ID:SGTC_1434|Compound:4073-0095|FD-Score:-4.82|P-value:2.86E-6 ID:SGTC_1435|Compound:4092-0789|FD-Score:4.35|P-value:2.17E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:4.12|P-value:5.41E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.90|P-value:1.24E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.83|P-value:2.81E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:4.64|P-value:6.50E-6 ID:SGTC_1638|Compound:st008370|FD-Score:4.96|P-value:1.56E-6 ID:SGTC_1655|Compound:st012546|FD-Score:3.44|P-value:6.13E-4 ID:SGTC_1684|Compound:st024538|FD-Score:3.35|P-value:8.28E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.57|P-value:3.82E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.82|P-value:1.67E-4 ID:SGTC_2073|Compound:5212005|FD-Score:-3.87|P-value:1.36E-4 ID:SGTC_2081|Compound:5214970|FD-Score:4.05|P-value:7.02E-5 ID:SGTC_2093|Compound:5331342|FD-Score:3.57|P-value:4.04E-4 ID:SGTC_2157|Compound:5649910|FD-Score:-4.16|P-value:4.44E-5 ID:SGTC_2308|Compound:7584454|FD-Score:4.68|P-value:5.54E-6 ID:SGTC_2370|Compound:9071495|FD-Score:3.88|P-value:1.35E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.36|P-value:7.82E-4 ID:SGTC_2412|Compound:st077135|FD-Score:-3.29|P-value:9.83E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.54|P-value:1.01E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.88|P-value:1.34E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:-3.36|P-value:7.88E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:-3.55|P-value:4.10E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.48|P-value:5.49E-4 ID:SGTC_2880|Compound:9042983|FD-Score:-4.27|P-value:2.87E-5 ID:SGTC_3007|Compound:9078383|FD-Score:6.24|P-value:2.34E-9 ID:SGTC_3018|Compound:9082602|FD-Score:-4.30|P-value:2.55E-5 ID:SGTC_3019|Compound:9081971|FD-Score:3.47|P-value:5.56E-4 ID:SGTC_33|Compound:0986-0033|FD-Score:3.88|P-value:1.33E-4 ID:SGTC_146|Compound:3448-8650|FD-Score:4.66|P-value:6.10E-6 ID:SGTC_276|Compound:colchicine|FD-Score:3.64|P-value:3.13E-4 ID:SGTC_299|Compound:1273-0059|FD-Score:-3.70|P-value:2.45E-4 ID:SGTC_304|Compound:1310-0298|FD-Score:-3.45|P-value:5.80E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.56|P-value:4.02E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:-4.40|P-value:1.70E-5 ID:SGTC_453|Compound:enantio-paf c16|FD-Score:-4.11|P-value:5.53E-5 ID:SGTC_503|Compound:tyrphostin 8|FD-Score:3.33|P-value:8.92E-4 ID:SGTC_545|Compound:4218-0007|FD-Score:3.75|P-value:2.13E-4 ID:SGTC_710|Compound:3448-4187|FD-Score:4.45|P-value:1.48E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:-3.43|P-value:6.14E-4 ID:SGTC_1130|Compound:3454-2858|FD-Score:3.30|P-value:9.67E-4 ID:SGTC_1408|Compound:3909-7976|FD-Score:6.94|P-value:3.41E-11 ID:SGTC_1430|Compound:4049-0248|FD-Score:-3.99|P-value:8.68E-5 ID:SGTC_1434|Compound:4073-0095|FD-Score:-4.82|P-value:2.86E-6 ID:SGTC_1435|Compound:4092-0789|FD-Score:4.35|P-value:2.17E-5 ID:SGTC_1454|Compound:4239-0054|FD-Score:4.12|P-value:5.41E-5 ID:SGTC_1529|Compound:apigenin|FD-Score:3.90|P-value:1.24E-4 ID:SGTC_1543|Compound:azetidyl-2-carboxylic acid|FD-Score:4.83|P-value:2.81E-6 ID:SGTC_1570|Compound:verbenone|FD-Score:4.64|P-value:6.50E-6 ID:SGTC_1638|Compound:st008370|FD-Score:4.96|P-value:1.56E-6 ID:SGTC_1655|Compound:st012546|FD-Score:3.44|P-value:6.13E-4 ID:SGTC_1684|Compound:st024538|FD-Score:3.35|P-value:8.28E-4 ID:SGTC_1745|Compound:st081495|FD-Score:-3.57|P-value:3.82E-4 ID:SGTC_1888|Compound:azatryptophan|FD-Score:3.82|P-value:1.67E-4 ID:SGTC_2073|Compound:5212005|FD-Score:-3.87|P-value:1.36E-4 ID:SGTC_2081|Compound:5214970|FD-Score:4.05|P-value:7.02E-5 ID:SGTC_2093|Compound:5331342|FD-Score:3.57|P-value:4.04E-4 ID:SGTC_2157|Compound:5649910|FD-Score:-4.16|P-value:4.44E-5 ID:SGTC_2308|Compound:7584454|FD-Score:4.68|P-value:5.54E-6 ID:SGTC_2370|Compound:9071495|FD-Score:3.88|P-value:1.35E-4 ID:SGTC_2387|Compound:9077560|FD-Score:-3.36|P-value:7.82E-4 ID:SGTC_2412|Compound:st077135|FD-Score:-3.29|P-value:9.83E-4 ID:SGTC_2502|Compound:avocadyne acetate|FD-Score:4.54|P-value:1.01E-5 ID:SGTC_2531|Compound:methyl deoxycholate|FD-Score:3.88|P-value:1.34E-4 ID:SGTC_2686|Compound:alizarin|FD-Score:-3.36|P-value:7.88E-4 ID:SGTC_2721|Compound:ifenprodil|FD-Score:-3.55|P-value:4.10E-4 ID:SGTC_2743|Compound:auranofin|FD-Score:3.48|P-value:5.49E-4 ID:SGTC_2880|Compound:9042983|FD-Score:-4.27|P-value:2.87E-5 ID:SGTC_3007|Compound:9078383|FD-Score:6.24|P-value:2.34E-9 ID:SGTC_3018|Compound:9082602|FD-Score:-4.30|P-value:2.55E-5 ID:SGTC_3019|Compound:9081971|FD-Score:3.47|P-value:5.56E-4

Top fitness defect scores for YHR021C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1408 3909-7976 6.94 48.8 µM 3.41E-11 Golgi 4 1.89E-12
2 SGTC_3007 9078383 6.24 71.4 µM 2.34E-9 3 2.24E-10
3 SGTC_1638 st008370 4.96 60.6 µM 1.56E-6 ergosterol biosynthesis 15 3.45E-7
4 SGTC_1543 azetidyl-2-carboxylic acid 4.83 198.0 µM 2.81E-6 4 6.69E-7
5 SGTC_2308 7584454 4.68 197.6 µM 5.54E-6 12 1.44E-6
6 SGTC_146 3448-8650 4.66 53.1 µM 6.10E-6 NEO1-PIK1 103 1.60E-6
7 SGTC_1570 verbenone 4.64 133.0 µM 6.50E-6 7 1.72E-6
8 SGTC_2502 avocadyne acetate 4.54 18.6 µM 1.01E-5 17 2.83E-6
9 SGTC_710 3448-4187 4.45 58.0 µM 1.48E-5 fatty acid desaturase (OLE1) 40 4.34E-6
10 SGTC_1435 4092-0789 4.35 56.7 µM 2.17E-5 plasma membrane duress 11 6.69E-6
11 SGTC_1454 4239-0054 4.12 42.6 µM 5.41E-5 29 1.87E-5
12 SGTC_2081 5214970 4.05 32.3 µM 7.02E-5 23 2.51E-5
13 SGTC_1529 apigenin 3.90 74.0 µM 1.24E-4 36 4.74E-5
14 SGTC_33 0986-0033 3.88 35.8 µM 1.33E-4 calcium & mitochondrial duress 22 5.13E-5
15 SGTC_2531 methyl deoxycholate 3.88 70.1 µM 1.34E-4 26 5.19E-5
16 SGTC_2370 9071495 3.88 200.0 µM 1.35E-4 mitochondrial processes 29 5.24E-5
17 SGTC_1888 azatryptophan 3.82 97.5 µM 1.67E-4 23 6.66E-5
18 SGTC_545 4218-0007 3.75 221.0 µM 2.13E-4 32 8.74E-5
19 SGTC_276 colchicine 3.64 750.0 µM 3.13E-4 22 1.35E-4
20 SGTC_2093 5331342 3.57 194.4 µM 4.04E-4 31 1.79E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.123 9.07E-13 YGR159C NSR1 hom Nucleolar protein that binds nuclear localization sequences, required for pre-rRNA processing and ribosome biogenesis
0.110 1.47E-10 YGL188C-A_p YGL188C-A_p hom Putative protein of unknown function
0.094 4.80E-8 YCR077C PAT1 hom Deadenylation-dependent mRNA-decapping factor; also required for faithful chromosome transmission, maintenance of rDNA locus stability, and protection of mRNA 3'-UTRs from trimming; associated with topoisomerase II; functionally linked to Pab1p; forms cytoplasmic foci upon DNA replication stress
0.093 6.70E-8 YBR189W RPS9B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S9 and bacterial S4; RPS9B has a paralog, RPS9A, that arose from the whole genome duplication
0.091 1.33E-7 YML062C MFT1 hom Subunit of the THO complex, which is a nuclear complex comprised of Hpr1p, Mft1p, Rlr1p, and Thp2p, that is involved in transcription elongation and mitotic recombination; involved in telomere maintenance
0.086 6.17E-7 YNL246W VPS75 hom NAP family histone chaperone; binds to histones and Rtt109p, stimulating histone acetyltransferase activity; possesses nucleosome assembly activity in vitro; proposed role in vacuolar protein sorting and in double-strand break repair; protein abundance increases in response to DNA replication stress
0.084 1.05E-6 YNR029C_p YNR029C_p hom Putative protein of unknown function, deletion confers reduced fitness in saline
0.083 1.36E-6 YDL083C RPS16B hom Protein component of the small (40S) ribosomal subunit; homologous to mammalian ribosomal protein S16 and bacterial S9; RPS16B has a paralog, RPS16A, that arose from the whole genome duplication
0.083 1.43E-6 YDL025C RTK1 hom Putative protein kinase, potentially phosphorylated by Cdc28p; interacts with ribosome biogenesis factors, Cka2, Gus1 and Arc1; protein abundance increases in response to DNA replication stress
0.083 1.55E-6 YOR016C ERP4 hom Protein with similarity to Emp24p and Erv25p, member of the p24 family involved in ER to Golgi transport
0.083 1.57E-6 YFR001W LOC1 hom Nuclear protein involved in asymmetric localization of ASH1 mRNA; binds double-stranded RNA in vitro; constituent of 66S pre-ribosomal particles; relocalizes from nucleus to cytoplasm upon DNA replication stress
0.080 3.37E-6 YBR111W-A SUS1 hom Component of both the SAGA histone acetylase and TREX-2 complexes; interacts with RNA polymerase II; involved in mRNA export coupled transcription activation and elongation; involved in post-transcriptional tethering of active genes to the nuclear periphery and to non-nascent mRNP
0.080 3.41E-6 YIL105C SLM1 hom Phosphoinositide PI4,5P(2) binding protein, forms a complex with Slm2p; acts downstream of Mss4p in a pathway regulating actin cytoskeleton organization in response to stress; phosphorylated by the TORC2 complex; protein abundance increases in response to DNA replication stress; SLM1 has a paralog, SLM2, that arose from the whole genome duplication
0.079 5.29E-6 YHR059W FYV4 hom Protein of unknown function, required for survival upon exposure to K1 killer toxin
0.078 5.69E-6 YOR309C_d YOR309C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene NOP58