YHR037W / PUT2

Delta-1-pyrroline-5-carboxylate dehydrogenase, nuclear-encoded mitochondrial protein involved in utilization of proline as sole nitrogen source; deficiency of the human homolog causes HPII, an autosomal recessive inborn error of metabolism

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR037W / PUT2

Click on Significant Values for Screen Details ID:SGTC_77|Compound:0828-0286|FD-Score:4.50|P-value:1.25E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:4.70|P-value:5.23E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:6.51|P-value:4.88E-10 ID:SGTC_166|Compound:k072-0058|FD-Score:3.35|P-value:8.64E-4 ID:SGTC_230|Compound:MPP+|FD-Score:4.96|P-value:1.67E-6 ID:SGTC_264|Compound:promethazine|FD-Score:6.69|P-value:1.74E-10 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.36|P-value:8.16E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:4.52|P-value:1.12E-5 ID:SGTC_730|Compound:1436-0052|FD-Score:3.40|P-value:7.21E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.47|P-value:5.73E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.50|P-value:5.19E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:6.54|P-value:4.16E-10 ID:SGTC_1034|Compound:k072-0165|FD-Score:-6.25|P-value:1.96E-9 ID:SGTC_1035|Compound:k081-0041|FD-Score:-5.28|P-value:3.22E-7 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.69|P-value:2.69E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.64|P-value:3.27E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.36|P-value:8.19E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:-3.35|P-value:7.84E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.50|P-value:5.17E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.63|P-value:3.38E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.58|P-value:4.01E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.59|P-value:8.25E-6 ID:SGTC_1949|Compound:st076024|FD-Score:3.83|P-value:1.69E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.80|P-value:1.87E-4 ID:SGTC_2071|Compound:5230951|FD-Score:3.91|P-value:1.25E-4 ID:SGTC_2088|Compound:5278280|FD-Score:4.22|P-value:3.78E-5 ID:SGTC_2093|Compound:5331342|FD-Score:4.33|P-value:2.45E-5 ID:SGTC_2159|Compound:5658611|FD-Score:3.94|P-value:1.10E-4 ID:SGTC_2347|Compound:9049783|FD-Score:4.18|P-value:4.50E-5 ID:SGTC_2362|Compound:9071156|FD-Score:3.52|P-value:4.92E-4 ID:SGTC_2365|Compound:9042475|FD-Score:3.76|P-value:2.12E-4 ID:SGTC_2394|Compound:5221648|FD-Score:-4.06|P-value:6.37E-5 ID:SGTC_2395|Compound:5646831|FD-Score:-4.09|P-value:5.78E-5 ID:SGTC_2400|Compound:5203494|FD-Score:3.83|P-value:1.63E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.36|P-value:2.19E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.45|P-value:6.24E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.39|P-value:7.45E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.33|P-value:9.12E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.38|P-value:7.82E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.50|P-value:5.29E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.35|P-value:8.46E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.34|P-value:8.02E-4 ID:SGTC_2957|Compound:9076800|FD-Score:-3.46|P-value:5.37E-4 ID:SGTC_3253|Compound:9135987|FD-Score:4.75|P-value:4.16E-6 ID:SGTC_3310|Compound:9130229|FD-Score:3.30|P-value:9.90E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.53|P-value:4.72E-4 ID:SGTC_77|Compound:0828-0286|FD-Score:4.50|P-value:1.25E-5 ID:SGTC_144|Compound:4161-2749|FD-Score:4.70|P-value:5.23E-6 ID:SGTC_145|Compound:3737-0113|FD-Score:6.51|P-value:4.88E-10 ID:SGTC_166|Compound:k072-0058|FD-Score:3.35|P-value:8.64E-4 ID:SGTC_230|Compound:MPP+|FD-Score:4.96|P-value:1.67E-6 ID:SGTC_264|Compound:promethazine|FD-Score:6.69|P-value:1.74E-10 ID:SGTC_452|Compound:diindolylmethane|FD-Score:3.36|P-value:8.16E-4 ID:SGTC_471|Compound:1-acyl-paf|FD-Score:4.52|P-value:1.12E-5 ID:SGTC_730|Compound:1436-0052|FD-Score:3.40|P-value:7.21E-4 ID:SGTC_786|Compound:4544-0069|FD-Score:3.47|P-value:5.73E-4 ID:SGTC_789|Compound:1309-1135|FD-Score:3.50|P-value:5.19E-4 ID:SGTC_1033|Compound:k072-0102|FD-Score:6.54|P-value:4.16E-10 ID:SGTC_1034|Compound:k072-0165|FD-Score:-6.25|P-value:1.96E-9 ID:SGTC_1035|Compound:k081-0041|FD-Score:-5.28|P-value:3.22E-7 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.69|P-value:2.69E-4 ID:SGTC_1156|Compound:4092-0367|FD-Score:3.64|P-value:3.27E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:3.36|P-value:8.19E-4 ID:SGTC_1283|Compound:0922-0125|FD-Score:-3.35|P-value:7.84E-4 ID:SGTC_1468|Compound:k284-1086|FD-Score:3.50|P-value:5.17E-4 ID:SGTC_1494|Compound:4358-1428|FD-Score:3.63|P-value:3.38E-4 ID:SGTC_1850|Compound:st056238|FD-Score:3.58|P-value:4.01E-4 ID:SGTC_1926|Compound:methyl fluorone black|FD-Score:4.59|P-value:8.25E-6 ID:SGTC_1949|Compound:st076024|FD-Score:3.83|P-value:1.69E-4 ID:SGTC_2070|Compound:5230947|FD-Score:3.80|P-value:1.87E-4 ID:SGTC_2071|Compound:5230951|FD-Score:3.91|P-value:1.25E-4 ID:SGTC_2088|Compound:5278280|FD-Score:4.22|P-value:3.78E-5 ID:SGTC_2093|Compound:5331342|FD-Score:4.33|P-value:2.45E-5 ID:SGTC_2159|Compound:5658611|FD-Score:3.94|P-value:1.10E-4 ID:SGTC_2347|Compound:9049783|FD-Score:4.18|P-value:4.50E-5 ID:SGTC_2362|Compound:9071156|FD-Score:3.52|P-value:4.92E-4 ID:SGTC_2365|Compound:9042475|FD-Score:3.76|P-value:2.12E-4 ID:SGTC_2394|Compound:5221648|FD-Score:-4.06|P-value:6.37E-5 ID:SGTC_2395|Compound:5646831|FD-Score:-4.09|P-value:5.78E-5 ID:SGTC_2400|Compound:5203494|FD-Score:3.83|P-value:1.63E-4 ID:SGTC_2405|Compound:ticlatone|FD-Score:4.36|P-value:2.19E-5 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:3.45|P-value:6.24E-4 ID:SGTC_2661|Compound:dactinomycin|FD-Score:3.39|P-value:7.45E-4 ID:SGTC_2663|Compound:amphotericin b|FD-Score:3.33|P-value:9.12E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:3.38|P-value:7.82E-4 ID:SGTC_2901|Compound:5-(ethoxymethyl)quinolin-8-ol|FD-Score:3.50|P-value:5.29E-4 ID:SGTC_2906|Compound:7926292|FD-Score:3.35|P-value:8.46E-4 ID:SGTC_2941|Compound:9056145|FD-Score:-3.34|P-value:8.02E-4 ID:SGTC_2957|Compound:9076800|FD-Score:-3.46|P-value:5.37E-4 ID:SGTC_3253|Compound:9135987|FD-Score:4.75|P-value:4.16E-6 ID:SGTC_3310|Compound:9130229|FD-Score:3.30|P-value:9.90E-4 ID:SGTC_3318|Compound:9139014|FD-Score:3.53|P-value:4.72E-4

Top fitness defect scores for YHR037W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_264 promethazine 6.69 12.5 mM 1.74E-10 DNA intercalators 17 1.14E-11
2 SGTC_1033 k072-0102 6.54 21.3 µM 4.16E-10 60S ribosome export 1 3.04E-11
3 SGTC_145 3737-0113 6.51 38.4 µM 4.88E-10 DNA intercalators 20 3.64E-11
4 SGTC_230 MPP+ 4.96 141.7 µM 1.67E-6 5 3.58E-7
5 SGTC_3253 9135987 4.75 49.5 µM 4.16E-6 60S ribosome export 4 1.00E-6
6 SGTC_144 4161-2749 4.70 112.4 µM 5.23E-6 DNA intercalators 15 1.29E-6
7 SGTC_1926 methyl fluorone black 4.59 53.6 µM 8.25E-6 DNA intercalators 44 2.17E-6
8 SGTC_471 1-acyl-paf 4.52 4.0 µM 1.12E-5 DNA intercalators 22 3.06E-6
9 SGTC_77 0828-0286 4.50 12.5 µM 1.25E-5 TSC3-RPN4 21 3.46E-6
10 SGTC_2405 ticlatone 4.36 3.5 µM 2.19E-5 52 6.53E-6
11 SGTC_2093 5331342 4.33 194.4 µM 2.45E-5 23 7.39E-6
12 SGTC_2088 5278280 4.22 127.4 µM 3.78E-5 17 1.21E-5
13 SGTC_2347 9049783 4.18 174.8 µM 4.50E-5 19 1.47E-5
14 SGTC_2159 5658611 3.94 73.8 µM 1.10E-4 10 4.04E-5
15 SGTC_2071 5230951 3.91 39.9 µM 1.25E-4 TSC3-RPN4 19 4.66E-5
16 SGTC_2400 5203494 3.83 178.7 µM 1.63E-4 RSC complex & mRNA processing 28 6.29E-5
17 SGTC_1949 st076024 3.83 69.1 µM 1.69E-4 28 6.52E-5
18 SGTC_2070 5230947 3.80 200.0 µM 1.87E-4 11 7.32E-5
19 SGTC_2365 9042475 3.76 200.0 µM 2.12E-4 41 8.45E-5
20 SGTC_1036 k216-0215 3.69 33.5 µM 2.69E-4 mitochondrial response to ROS 63 1.10E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.331 4.90E-86 YMR289W ABZ2 hom Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.290 8.94E-66 YMR166C_p YMR166C_p hom Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.285 9.10E-64 YMR168C CEP3 het Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.268 2.82E-56 YHL039W EFM1 hom Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.267 1.20E-55 YGL118C_d YGL118C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.258 6.11E-52 YDL223C HBT1 hom Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.232 5.30E-42 YOR133W EFT1 hom Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.215 2.22E-36 YPL157W TGS1 hom Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs
0.207 1.10E-33 YJL025W RRN7 het Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.203 2.04E-32 YEL032W MCM3 het Protein involved in DNA replication; component of the Mcm2-7 hexameric complex that binds chromatin as a part of the pre-replicative complex
0.203 2.15E-32 YGR065C VHT1 het High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.202 5.31E-32 YGL200C EMP24 hom Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.196 1.89E-30 YMR262W_p YMR262W_p hom Protein of unknown function; interacts weakly with Knr4p; YMR262W is not an essential gene
0.193 2.73E-29 YGL041C_d YGL041C_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.181 4.19E-26 YDR401W_d YDR401W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data