HIPHOP chemogenomics database

YHR038W / RRF1

Mitochondrial ribosome recycling factor, essential for mitochondrial protein synthesis and for the maintenance of the respiratory function of mitochondria

Zygosity: Homozygous strain

fixedexpanded
Profile for YHR038W / RRF1

Click on Significant Values for Screen Details

Top fitness defect scores for YHR038W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value
1	SGTC_310	1273-0058	9.90	1.1 µM	1.02E-20	mitochondrial stress	42	2.06E-23
2	SGTC_1427	4048-4900	9.76	550.0 nM	3.49E-20		7	8.42E-23
3	SGTC_275	trichlorophene	6.60	5.7 µM	3.45E-10	mitochondrial stress	99	2.11E-11
4	SGTC_236	1509-0011	5.91	5.3 µM	1.65E-8		296	1.73E-9
5	SGTC_2232	chlorophetanol	5.80	41.5 µM	2.98E-8	TRP & mitochondrial translation	45	3.39E-9
6	SGTC_2792	7535235	5.46	81.8 µM	1.65E-7	fatty acid desaturase (OLE1)	12	2.39E-8
7	SGTC_28	pentachloropyridine N-oxide	5.00	1.6 µM	1.47E-6	TSC3-RPN4	29	2.87E-7
8	SGTC_1107	amlodipine	4.87	47.0 µM	2.65E-6		17	5.61E-7
9	SGTC_883	0850-0245	4.83	24.6 µM	3.14E-6	cell wall	14	6.79E-7
10	SGTC_1440	4100-1550	4.82	40.0 µM	3.34E-6		1	7.30E-7
11	SGTC_892	0929-0076	4.81	29.0 µM	3.42E-6	TRP & mitochondrial translation	19	7.51E-7
12	SGTC_2797	7859098	4.74	81.8 µM	4.70E-6		13	1.08E-6
13	SGTC_1742	st038235	4.73	28.9 µM	4.86E-6	mitochondrial stress	87	1.12E-6
14	SGTC_1321	1326-2144	4.52	215.0 µM	1.18E-5		4	3.06E-6
15	SGTC_1442	4112-3290	4.47	3.0 µM	1.47E-5		3	3.93E-6
16	SGTC_1912	st060209	4.25	90.8 µM	3.48E-5		32	1.05E-5
17	SGTC_10	0279-0212	4.21	42.6 µM	4.22E-5	TRP & mitochondrial translation	109	1.30E-5
18	SGTC_1627	st003713	4.16	25.6 µM	4.94E-5	calcium & mitochondrial duress	65	1.56E-5
19	SGTC_63	0099-0242	4.07	26.7 µM	7.03E-5	mitochondrial stress	83	2.33E-5
20	SGTC_1623	st003709	4.06	20.3 µM	7.24E-5	calcium & mitochondrial duress	67	2.41E-5

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.372	7.35E-111	YLR091W	GEP5	hom	Protein of unknown function, required for mitochondrial genome maintenance; detected in highly purified mitochondria in high-throughput studies; null mutant has decreased levels of cardiolipin and phosphatidylethanolamine
0.363	4.02E-105	YMR228W	MTF1	hom	Mitochondrial RNA polymerase specificity factor; has structural similarity to S-adenosylmethionine-dependent methyltransferases and functional similarity to bacterial sigma-factors; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.353	1.29E-98	YLR260W	LCB5	hom	Minor sphingoid long-chain base kinase, paralog of Lcb4p responsible for few percent of the total activity, possibly involved in synthesis of long-chain base phosphates, which function as signaling molecules
0.347	1.70E-95	YER077C_p	YER077C_p	hom	Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion; null mutation results in a decrease in plasma membrane electron transport
0.346	6.35E-95	YMR193W	MRPL24	hom	Mitochondrial ribosomal protein of the large subunit; two mitochondrial ribosomal proteins, YmL14 and YmL24, have been assigned to the same gene
0.344	9.69E-94	YHR168W	MTG2	hom	Putative GTPase, member of the Obg family; peripheral protein of the mitochondrial inner membrane that associates with the large ribosomal subunit; required for mitochondrial translation, possibly via a role in ribosome assembly
0.341	2.64E-92	YPL040C	ISM1	hom	Mitochondrial isoleucyl-tRNA synthetase, null mutant is deficient in respiratory growth
0.336	3.15E-89	YGR219W_d	YGR219W_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF MRPL9/YGR220C
0.335	1.48E-88	YPL183W-A	RTC6	hom	Protein involved in translation; mutants have defects in biogenesis of nuclear ribosomes; sequence similar to prokaryotic ribosomal protein L36, may be a mitochondrial ribosomal protein; protein abundance increases in response to DNA replication stress
0.332	5.63E-87	YMR287C	DSS1	hom	3'-5' exoribonuclease, component of the mitochondrial degradosome along with the ATP-dependent RNA helicase Suv3p; the degradosome associates with the ribosome and mediates turnover of aberrant or unprocessed RNAs
0.331	2.42E-86	YLR067C	PET309	hom	Specific translational activator for the COX1 mRNA, also influences stability of intron-containing COX1 primary transcripts; localizes to the mitochondrial inner membrane; contains seven pentatricopeptide repeats (PPRs)
0.326	3.82E-84	YER050C	RSM18	hom	Mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S18 ribosomal protein
0.326	1.08E-83	YGR215W	RSM27	hom	Mitochondrial ribosomal protein of the small subunit
0.319	2.61E-80	YMR267W	PPA2	hom	Mitochondrial inorganic pyrophosphatase, required for mitochondrial function and possibly involved in energy generation from inorganic pyrophosphate
0.319	3.53E-80	YNL081C	SWS2	hom	Putative mitochondrial ribosomal protein of the small subunit, has similarity to E. coli S13 ribosomal protein; participates in controlling sporulation efficiency