YHR058C / MED6

Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; protein abundance increases in response to DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR058C / MED6

Click on Significant Values for Screen Details ID:SGTC_1|Compound:0258-0098|FD-Score:-3.83|P-value:1.75E-4 ID:SGTC_5|Compound:0352-0601|FD-Score:-3.98|P-value:1.03E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.94|P-value:1.21E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.97|P-value:1.05E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.72|P-value:5.29E-6 ID:SGTC_90|Compound:0870-0863|FD-Score:4.81|P-value:3.62E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:4.49|P-value:1.42E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:3.87|P-value:1.53E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-4.18|P-value:4.88E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:3.34|P-value:9.18E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:9.69|P-value:7.96E-20 ID:SGTC_164|Compound:4100-3994|FD-Score:3.93|P-value:1.22E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:4.55|P-value:1.08E-5 ID:SGTC_252|Compound:miconazole|FD-Score:3.80|P-value:1.96E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.54|P-value:1.13E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.77|P-value:2.19E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.70|P-value:5.94E-6 ID:SGTC_338|Compound:4055-0136|FD-Score:-3.52|P-value:5.11E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.60|P-value:3.93E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:7.14|P-value:1.34E-11 ID:SGTC_556|Compound:4058-0063|FD-Score:4.17|P-value:4.95E-5 ID:SGTC_580|Compound:1123-0028|FD-Score:3.71|P-value:2.67E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:4.94|P-value:2.00E-6 ID:SGTC_648|Compound:0375-0473|FD-Score:-3.40|P-value:7.66E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:7.31|P-value:4.60E-12 ID:SGTC_709|Compound:3807-1509|FD-Score:3.91|P-value:1.33E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:-3.66|P-value:3.18E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.92|P-value:1.28E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:4.25|P-value:3.64E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:6.07|P-value:7.63E-9 ID:SGTC_931|Compound:2812-0076|FD-Score:3.59|P-value:4.04E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-5.04|P-value:1.30E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:4.32|P-value:2.78E-5 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.41|P-value:7.39E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-5.68|P-value:5.81E-8 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.76|P-value:2.30E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.58|P-value:4.22E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.76|P-value:2.27E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-4.26|P-value:3.61E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.35|P-value:8.79E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.93|P-value:1.21E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-4.12|P-value:6.02E-5 ID:SGTC_1323|Compound:1348-1351|FD-Score:-3.55|P-value:4.70E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.72|P-value:2.56E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.38|P-value:2.60E-7 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.00|P-value:1.54E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.55|P-value:4.70E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:5.56|P-value:1.06E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:-3.69|P-value:2.95E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.40|P-value:7.68E-4 ID:SGTC_1652|Compound:st011932|FD-Score:4.25|P-value:3.64E-5 ID:SGTC_1687|Compound:st020778|FD-Score:3.35|P-value:8.81E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.78|P-value:2.14E-4 ID:SGTC_1751|Compound:st044839|FD-Score:3.68|P-value:2.95E-4 ID:SGTC_1765|Compound:st044815|FD-Score:6.59|P-value:3.96E-10 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.80|P-value:1.98E-4 ID:SGTC_1904|Compound:st060225|FD-Score:-3.98|P-value:1.04E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.80|P-value:1.99E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-4.24|P-value:3.92E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.56|P-value:4.49E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.69|P-value:5.97E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-3.79|P-value:2.03E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.99|P-value:9.84E-5 ID:SGTC_2396|Compound:5342950|FD-Score:8.03|P-value:3.46E-14 ID:SGTC_2401|Compound:5128683|FD-Score:3.34|P-value:9.28E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.48|P-value:1.44E-5 ID:SGTC_2475|Compound:5812728|FD-Score:4.51|P-value:1.27E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-6.29|P-value:2.21E-9 ID:SGTC_2676|Compound:harmalol|FD-Score:7.23|P-value:7.75E-12 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.48|P-value:5.88E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.56|P-value:4.47E-4 ID:SGTC_2929|Compound:9013348|FD-Score:-4.83|P-value:3.41E-6 ID:SGTC_2932|Compound:9016711|FD-Score:3.83|P-value:1.78E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-5.68|P-value:6.06E-8 ID:SGTC_2938|Compound:9059746|FD-Score:6.90|P-value:6.24E-11 ID:SGTC_2996|Compound:9066192|FD-Score:3.48|P-value:5.86E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.75|P-value:2.31E-4 ID:SGTC_3199|Compound:9111318|FD-Score:3.66|P-value:3.14E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.72|P-value:2.60E-4 ID:SGTC_1|Compound:0258-0098|FD-Score:-3.83|P-value:1.75E-4 ID:SGTC_5|Compound:0352-0601|FD-Score:-3.98|P-value:1.03E-4 ID:SGTC_36|Compound:0335-0881|FD-Score:3.94|P-value:1.21E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.97|P-value:1.05E-4 ID:SGTC_64|Compound:000l-0018|FD-Score:4.72|P-value:5.29E-6 ID:SGTC_90|Compound:0870-0863|FD-Score:4.81|P-value:3.62E-6 ID:SGTC_119|Compound:0121-0043|FD-Score:4.49|P-value:1.42E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:3.87|P-value:1.53E-4 ID:SGTC_129|Compound:0088-0017|FD-Score:-4.18|P-value:4.88E-5 ID:SGTC_134|Compound:0269-0018|FD-Score:3.34|P-value:9.18E-4 ID:SGTC_157|Compound:1348-1695|FD-Score:9.69|P-value:7.96E-20 ID:SGTC_164|Compound:4100-3994|FD-Score:3.93|P-value:1.22E-4 ID:SGTC_227|Compound:fluconazole|FD-Score:4.55|P-value:1.08E-5 ID:SGTC_252|Compound:miconazole|FD-Score:3.80|P-value:1.96E-4 ID:SGTC_256|Compound:clotrimazole|FD-Score:4.54|P-value:1.13E-5 ID:SGTC_263|Compound:alizarin|FD-Score:3.77|P-value:2.19E-4 ID:SGTC_268|Compound:camptothecin|FD-Score:4.70|P-value:5.94E-6 ID:SGTC_338|Compound:4055-0136|FD-Score:-3.52|P-value:5.11E-4 ID:SGTC_434|Compound:1-hexadecyl-2-methylglycero-3 PC|FD-Score:3.60|P-value:3.93E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:7.14|P-value:1.34E-11 ID:SGTC_556|Compound:4058-0063|FD-Score:4.17|P-value:4.95E-5 ID:SGTC_580|Compound:1123-0028|FD-Score:3.71|P-value:2.67E-4 ID:SGTC_628|Compound:1636-0253|FD-Score:4.94|P-value:2.00E-6 ID:SGTC_648|Compound:0375-0473|FD-Score:-3.40|P-value:7.66E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:7.31|P-value:4.60E-12 ID:SGTC_709|Compound:3807-1509|FD-Score:3.91|P-value:1.33E-4 ID:SGTC_729|Compound:4092-0585|FD-Score:-3.66|P-value:3.18E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:3.92|P-value:1.28E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:4.25|P-value:3.64E-5 ID:SGTC_839|Compound:0122-0028|FD-Score:6.07|P-value:7.63E-9 ID:SGTC_931|Compound:2812-0076|FD-Score:3.59|P-value:4.04E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-5.04|P-value:1.30E-6 ID:SGTC_996|Compound:3970-0799|FD-Score:4.32|P-value:2.78E-5 ID:SGTC_1001|Compound:4048-4355|FD-Score:-3.41|P-value:7.39E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-5.68|P-value:5.81E-8 ID:SGTC_1028|Compound:k015-0029|FD-Score:-3.76|P-value:2.30E-4 ID:SGTC_1029|Compound:k015-0030|FD-Score:3.58|P-value:4.22E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.76|P-value:2.27E-4 ID:SGTC_1129|Compound:3453-1384|FD-Score:-4.26|P-value:3.61E-5 ID:SGTC_1151|Compound:3909-8316|FD-Score:3.35|P-value:8.79E-4 ID:SGTC_1305|Compound:1181-0477|FD-Score:3.93|P-value:1.21E-4 ID:SGTC_1310|Compound:1188-0297|FD-Score:-4.12|P-value:6.02E-5 ID:SGTC_1323|Compound:1348-1351|FD-Score:-3.55|P-value:4.70E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.72|P-value:2.56E-4 ID:SGTC_1454|Compound:4239-0054|FD-Score:5.38|P-value:2.60E-7 ID:SGTC_1462|Compound:k081-0014|FD-Score:-5.00|P-value:1.54E-6 ID:SGTC_1469|Compound:k284-2957|FD-Score:3.55|P-value:4.70E-4 ID:SGTC_1493|Compound:4348-0038|FD-Score:5.56|P-value:1.06E-7 ID:SGTC_1510|Compound:1123-0200|FD-Score:-3.69|P-value:2.95E-4 ID:SGTC_1547|Compound:harmine|FD-Score:3.40|P-value:7.68E-4 ID:SGTC_1652|Compound:st011932|FD-Score:4.25|P-value:3.64E-5 ID:SGTC_1687|Compound:st020778|FD-Score:3.35|P-value:8.81E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:-3.78|P-value:2.14E-4 ID:SGTC_1751|Compound:st044839|FD-Score:3.68|P-value:2.95E-4 ID:SGTC_1765|Compound:st044815|FD-Score:6.59|P-value:3.96E-10 ID:SGTC_1844|Compound:rapamycin|FD-Score:-3.80|P-value:1.98E-4 ID:SGTC_1904|Compound:st060225|FD-Score:-3.98|P-value:1.04E-4 ID:SGTC_2005|Compound:4024620|FD-Score:-3.80|P-value:1.99E-4 ID:SGTC_2006|Compound:4011487|FD-Score:-4.24|P-value:3.92E-5 ID:SGTC_2111|Compound:5486399|FD-Score:3.56|P-value:4.49E-4 ID:SGTC_2286|Compound:7945697|FD-Score:4.69|P-value:5.97E-6 ID:SGTC_2320|Compound:7373003|FD-Score:-3.79|P-value:2.03E-4 ID:SGTC_2393|Compound:9031399|FD-Score:3.99|P-value:9.84E-5 ID:SGTC_2396|Compound:5342950|FD-Score:8.03|P-value:3.46E-14 ID:SGTC_2401|Compound:5128683|FD-Score:3.34|P-value:9.28E-4 ID:SGTC_2402|Compound:5553605|FD-Score:4.48|P-value:1.44E-5 ID:SGTC_2475|Compound:5812728|FD-Score:4.51|P-value:1.27E-5 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-6.29|P-value:2.21E-9 ID:SGTC_2676|Compound:harmalol|FD-Score:7.23|P-value:7.75E-12 ID:SGTC_2757|Compound:clomiphene|FD-Score:-3.48|P-value:5.88E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.56|P-value:4.47E-4 ID:SGTC_2929|Compound:9013348|FD-Score:-4.83|P-value:3.41E-6 ID:SGTC_2932|Compound:9016711|FD-Score:3.83|P-value:1.78E-4 ID:SGTC_2934|Compound:9036370|FD-Score:-5.68|P-value:6.06E-8 ID:SGTC_2938|Compound:9059746|FD-Score:6.90|P-value:6.24E-11 ID:SGTC_2996|Compound:9066192|FD-Score:3.48|P-value:5.86E-4 ID:SGTC_2997|Compound:9067075|FD-Score:3.75|P-value:2.31E-4 ID:SGTC_3199|Compound:9111318|FD-Score:3.66|P-value:3.14E-4 ID:SGTC_3244|Compound:9134489|FD-Score:3.72|P-value:2.60E-4

Top fitness defect scores for YHR058C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_157 1348-1695 9.69 65.8 µM 7.96E-20 iron homeostasis 5 1.67E-22 0.24
2 SGTC_2396 5342950 8.03 42.8 µM 3.46E-14 RSC complex & mRNA processing 9 4.91E-16 0.48
3 SGTC_673 1082-0474 7.31 110.0 µM 4.60E-12 RSC complex & mRNA processing 22 1.34E-13 0.47
4 SGTC_2676 harmalol 7.23 100.0 µM 7.75E-12 mitochondrial processes 1 2.43E-13 1.64
5 SGTC_513 4245-1575 7.14 41.7 µM 1.34E-11 unfolded protein response 7 4.55E-13 0.93
6 SGTC_2938 9059746 6.90 27.3 µM 6.24E-11 fatty acid desaturase (OLE1) 2 2.66E-12 0.13
7 SGTC_1765 st044815 6.59 90.4 µM 3.96E-10 1 2.21E-11 1.22
8 SGTC_839 0122-0028 6.07 230.0 µM 7.63E-9 RSC complex & mRNA processing 12 6.55E-10 0.18
9 SGTC_1493 4348-0038 5.56 111.0 µM 1.06E-7 RSC complex & mRNA processing 20 1.33E-8 0.06
10 SGTC_1454 4239-0054 5.38 42.6 µM 2.60E-7 1 3.72E-8 0.62
11 SGTC_628 1636-0253 4.94 16.0 µM 2.00E-6 sphingolipid biosynthesis & PDR1 2 3.84E-7 0.11
12 SGTC_90 0870-0863 4.81 4.6 µM 3.62E-6 3 7.56E-7 0.68
13 SGTC_64 000l-0018 4.72 272.1 µM 5.29E-6 ubiquinone biosynthesis & proteasome 36 1.17E-6 0.08
14 SGTC_268 camptothecin 4.70 39.6 µM 5.94E-6 DNA damage response 33 1.33E-6 0.05
15 SGTC_2286 7945697 4.69 125.0 µM 5.97E-6 6 1.34E-6 0.08
16 SGTC_227 fluconazole 4.55 33.5 µM 1.08E-5 azole & statin 40 2.63E-6 0.02
17 SGTC_256 clotrimazole 4.54 625.0 nM 1.13E-5 ergosterol depletion effects on membrane 14 2.79E-6 0.18
18 SGTC_2475 5812728 4.51 15.0 µM 1.27E-5 mitochondrial processes 13 3.19E-6 0.16
19 SGTC_119 0121-0043 4.49 24.8 µM 1.42E-5 mitochondrial stress 13 3.61E-6 0.09
20 SGTC_2402 5553605 4.48 200.0 µM 1.44E-5 2 3.68E-6 0.04

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.272 7.96E-58 YPL242C IQG1 het Essential protein required for determination of budding pattern; promotes localization of axial markers Bud4p and Cdc12p and functionally interacts with Sec3p, localizes to the contractile ring during anaphase, member of the IQGAP family; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.232 2.65E-42 YPR104C FHL1 het Regulator of ribosomal protein (RP) transcription; has forkhead associated domain that binds phosphorylated proteins; recruits coactivator Ifh1p or corepressor Crf1p to RP gene promoters; also has forkhead DNA-binding domain though in vitro DNA binding assays give inconsistent results; computational analyses suggest it binds DNA directly at highly active RP genes and indirectly through Rap1p motifs at others; suppresses RNA pol III and splicing factor prp4 mutants
0.218 2.26E-37 YNR016C ACC1 het Acetyl-CoA carboxylase, biotin containing enzyme; catalyzes the carboxylation of cytosolic acetyl-CoA to form malonyl-CoA and regulates histone acetylation by regulating the availablity of acetyl-CoA; required for de novo biosynthesis of long-chain fatty acids;
0.192 2.90E-29 YMR277W FCP1 het Carboxy-terminal domain (CTD) phosphatase, essential for dephosphorylation of the repeated C-terminal domain of the RNA polymerase II large subunit (Rpo21p)
0.192 3.07E-29 YER022W SRB4 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; homozygosity of the human MED17 L371P mutation is associated with infantile cerebral and cerebellar atrophy with poor myelination
0.191 9.17E-29 YDR172W SUP35 het Translation termination factor eRF3, has a role in mRNA deadenylation and decay; altered protein conformation creates the [PSI(+)] prion that alters translational fidelity and results in a nonsense suppressor phenotype
0.189 2.73E-28 YBR143C SUP45 het Polypeptide release factor (eRF1) in translation termination; mutant form acts as a recessive omnipotent suppressor; methylated by Mtq2p-Trm112p in ternary complex eRF1-eRF3-GTP; mutation of methylation site confers resistance to zymocin; has a role in cytokinesis through interaction with Mlc1p
0.182 3.09E-26 YGL093W SPC105 het Subunit of a kinetochore-microtubule binding complex with Kre28p that bridges centromeric heterochromatin and kinetochore MAPs and motors; required for sister chromatid bi-orientation and kinetochore binding of SAC components
0.170 4.10E-23 YPR107C YTH1 het Essential RNA-binding component of cleavage and polyadenylation factor, contains five zinc fingers; required for pre-mRNA 3'-end processing and polyadenylation
0.169 8.36E-23 YJL081C ARP4 het Nuclear actin-related protein involved in chromatin remodeling, component of chromatin-remodeling enzyme complexes
0.166 3.98E-22 YOR236W DFR1 het Dihydrofolate reductase involved in tetrahydrofolate biosynthesis; required for respiratory metabolism
0.165 9.28E-22 YML127W RSC9 het Component of the RSC chromatin remodeling complex; DNA-binding protein involved in the synthesis of rRNA and in transcriptional repression and activation of genes regulated by the Target of Rapamycin (TOR) pathway
0.164 1.32E-21 YJL195C_d YJL195C_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YJL194W/CDC6
0.164 1.47E-21 YLR033W RSC58 het Component of the RSC chromatin remodeling complex; RSC functions in transcriptional regulation and elongation, chromosome stability, and establishing sister chromatid cohesion; involved in telomere maintenance
0.164 1.58E-21 YPR034W ARP7 het Component of both the SWI/SNF and RSC chromatin remodeling complexes; actin-related protein involved in transcriptional regulation