YHR060W / VMA22

Peripheral membrane protein that is required for vacuolar H+-ATPase (V-ATPase) function, although not an actual component of the V-ATPase complex; functions in the assembly of the V-ATPase; localized to the yeast endoplasmic reticulum (ER)

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR060W / VMA22

Click on Significant Values for Screen Details ID:SGTC_180|Compound:1486-1293|FD-Score:3.94|P-value:3.30E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:3.07|P-value:9.71E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.43|P-value:2.67E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.13|P-value:6.44E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.60|P-value:1.35E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.50|P-value:1.97E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.75|P-value:5.68E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.19|P-value:5.19E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.10|P-value:8.82E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:-8.24|P-value:1.60E-17 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.29|P-value:3.55E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-5.18|P-value:5.28E-8 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.21|P-value:5.99E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:-3.64|P-value:9.05E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.55|P-value:1.68E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.01|P-value:9.71E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.75|P-value:5.69E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.90|P-value:3.08E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.09|P-value:9.03E-4 ID:SGTC_1684|Compound:st024538|FD-Score:3.51|P-value:1.90E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.75|P-value:7.44E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.56|P-value:1.56E-4 ID:SGTC_1970|Compound:st070122|FD-Score:4.08|P-value:1.82E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.13|P-value:7.79E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.92|P-value:3.62E-5 ID:SGTC_2144|Compound:5628481|FD-Score:3.33|P-value:3.87E-4 ID:SGTC_2378|Compound:9074213|FD-Score:3.54|P-value:1.71E-4 ID:SGTC_2397|Compound:5161689|FD-Score:4.11|P-value:1.61E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.80|P-value:6.07E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.13|P-value:7.73E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.76|P-value:7.17E-5 ID:SGTC_2705|Compound:st078069|FD-Score:3.94|P-value:3.27E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-4.22|P-value:7.19E-6 ID:SGTC_2897|Compound:9048598|FD-Score:3.23|P-value:5.47E-4 ID:SGTC_2931|Compound:9014674|FD-Score:3.30|P-value:4.19E-4 ID:SGTC_2988|Compound:9073640|FD-Score:-3.01|P-value:9.68E-4 ID:SGTC_3074|Compound:9116018|FD-Score:3.19|P-value:6.30E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.45|P-value:2.44E-4 ID:SGTC_3181|Compound:9108277|FD-Score:4.14|P-value:1.36E-5 ID:SGTC_3322|Compound:9139058|FD-Score:-4.97|P-value:1.74E-7 ID:SGTC_180|Compound:1486-1293|FD-Score:3.94|P-value:3.30E-5 ID:SGTC_288|Compound:1315-0019|FD-Score:3.07|P-value:9.71E-4 ID:SGTC_290|Compound:2043-6722|FD-Score:3.43|P-value:2.67E-4 ID:SGTC_335|Compound:4077-0256|FD-Score:-3.13|P-value:6.44E-4 ID:SGTC_620|Compound:0109-0052|FD-Score:3.60|P-value:1.35E-4 ID:SGTC_622|Compound:1348-1566|FD-Score:3.50|P-value:1.97E-4 ID:SGTC_627|Compound:1391-0720|FD-Score:-3.75|P-value:5.68E-5 ID:SGTC_644|Compound:1498-1197|FD-Score:-3.19|P-value:5.19E-4 ID:SGTC_833|Compound:3852-0053|FD-Score:3.10|P-value:8.82E-4 ID:SGTC_883|Compound:0850-0245|FD-Score:-8.24|P-value:1.60E-17 ID:SGTC_893|Compound:0958-0369|FD-Score:-3.29|P-value:3.55E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:-5.18|P-value:5.28E-8 ID:SGTC_1096|Compound:rimcazole|FD-Score:3.21|P-value:5.99E-4 ID:SGTC_1212|Compound:0520-0165|FD-Score:-3.64|P-value:9.05E-5 ID:SGTC_1239|Compound:0337-0584|FD-Score:3.55|P-value:1.68E-4 ID:SGTC_1264|Compound:0783-0040|FD-Score:-3.01|P-value:9.71E-4 ID:SGTC_1388|Compound:3346-4487|FD-Score:-3.75|P-value:5.69E-5 ID:SGTC_1568|Compound:digitonin|FD-Score:-3.90|P-value:3.08E-5 ID:SGTC_1569|Compound:carminic acid|FD-Score:3.09|P-value:9.03E-4 ID:SGTC_1684|Compound:st024538|FD-Score:3.51|P-value:1.90E-4 ID:SGTC_1746|Compound:st038117|FD-Score:3.75|P-value:7.44E-5 ID:SGTC_1748|Compound:st037455|FD-Score:3.56|P-value:1.56E-4 ID:SGTC_1970|Compound:st070122|FD-Score:4.08|P-value:1.82E-5 ID:SGTC_1981|Compound:st071826|FD-Score:3.13|P-value:7.79E-4 ID:SGTC_2083|Compound:5306046|FD-Score:3.92|P-value:3.62E-5 ID:SGTC_2144|Compound:5628481|FD-Score:3.33|P-value:3.87E-4 ID:SGTC_2378|Compound:9074213|FD-Score:3.54|P-value:1.71E-4 ID:SGTC_2397|Compound:5161689|FD-Score:4.11|P-value:1.61E-5 ID:SGTC_2406|Compound:ethylparaben|FD-Score:3.80|P-value:6.07E-5 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:3.13|P-value:7.73E-4 ID:SGTC_2615|Compound:purpurogallin-4-carboxylic acid|FD-Score:3.76|P-value:7.17E-5 ID:SGTC_2705|Compound:st078069|FD-Score:3.94|P-value:3.27E-5 ID:SGTC_2887|Compound:9044784|FD-Score:-4.22|P-value:7.19E-6 ID:SGTC_2897|Compound:9048598|FD-Score:3.23|P-value:5.47E-4 ID:SGTC_2931|Compound:9014674|FD-Score:3.30|P-value:4.19E-4 ID:SGTC_2988|Compound:9073640|FD-Score:-3.01|P-value:9.68E-4 ID:SGTC_3074|Compound:9116018|FD-Score:3.19|P-value:6.30E-4 ID:SGTC_3126|Compound:9127922|FD-Score:3.45|P-value:2.44E-4 ID:SGTC_3181|Compound:9108277|FD-Score:4.14|P-value:1.36E-5 ID:SGTC_3322|Compound:9139058|FD-Score:-4.97|P-value:1.74E-7

Top fitness defect scores for YHR060W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3181 9108277 4.14 49.5 µM 1.36E-5 iron homeostasis 11 1.70E-5
2 SGTC_2397 5161689 4.11 200.0 µM 1.61E-5 29 2.02E-5
3 SGTC_1970 st070122 4.08 35.8 µM 1.82E-5 TSC3-RPN4 24 2.26E-5
4 SGTC_2705 st078069 3.94 42.8 µM 3.27E-5 6 4.01E-5
5 SGTC_180 1486-1293 3.94 102.8 µM 3.30E-5 42 4.03E-5
6 SGTC_2083 5306046 3.92 65.9 µM 3.62E-5 28 4.42E-5
7 SGTC_2406 ethylparaben 3.80 405.7 µM 6.07E-5 46 7.30E-5
8 SGTC_2615 purpurogallin-4-carboxylic acid 3.76 100.0 µM 7.17E-5 redox potentiating 70 8.57E-5
9 SGTC_1746 st038117 3.75 42.0 µM 7.44E-5 38 8.88E-5
10 SGTC_620 0109-0052 3.60 432.0 µM 1.35E-4 29 1.58E-4
11 SGTC_1748 st037455 3.56 53.6 µM 1.56E-4 43 1.83E-4
12 SGTC_1239 0337-0584 3.55 35.4 µM 1.68E-4 unfolded protein response 72 1.95E-4
13 SGTC_2378 9074213 3.54 121.4 µM 1.71E-4 RSC complex & mRNA processing 31 1.99E-4
14 SGTC_1684 st024538 3.51 14.6 µM 1.90E-4 11 2.21E-4
15 SGTC_622 1348-1566 3.50 26.3 µM 1.97E-4 32 2.29E-4
16 SGTC_3126 9127922 3.45 49.5 µM 2.44E-4 fatty acid desaturase (OLE1) 55 2.81E-4
17 SGTC_290 2043-6722 3.43 48.1 µM 2.67E-4 22 3.07E-4
18 SGTC_2144 5628481 3.33 200.0 µM 3.87E-4 ERG2 46 4.40E-4
19 SGTC_2931 9014674 3.30 55.7 µM 4.19E-4 47 4.76E-4
20 SGTC_2897 9048598 3.23 13.0 µM 5.47E-4 40 6.16E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.108 2.93E-10 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.095 4.02E-8 YGR197C SNG1 hom Protein involved in resistance to nitrosoguanidine (MNNG) and 6-azauracil (6-AU); expression is regulated by transcription factors involved in multidrug resistance
0.090 1.88E-7 YFL032W_d YFL032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene HAC1/YFL031W; YFL032W is not an essential gene
0.087 4.86E-7 YLR312W-A MRPL15 hom Mitochondrial ribosomal protein of the large subunit
0.083 1.55E-6 YJR141W_p YJR141W_p het Essential protein of unknown function
0.081 2.81E-6 YPR138C MEP3 hom Ammonium permease of high capacity and low affinity; belongs to a ubiquitous family of cytoplasmic membrane proteins that transport only ammonium (NH4+); expression is under the nitrogen catabolite repression regulation ammonia permease
0.077 8.88E-6 YMR090W_p YMR090W_p hom Putative protein of unknown function with similarity to DTDP-glucose 4,6-dehydratases; GFP-fusion protein localizes to the cytoplasm; up-regulated in response to the fungicide mancozeb; not essential for viability
0.076 9.91E-6 YGL038C OCH1 hom Mannosyltransferase of the cis-Golgi apparatus, initiates the polymannose outer chain elongation of N-linked oligosaccharides of glycoproteins
0.074 2.24E-5 YKR099W BAS1 hom Myb-related transcription factor involved in regulating basal and induced expression of genes of the purine and histidine biosynthesis pathways; also involved in regulation of meiotic recombination at specific genes
0.074 1.67E-5 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.074 1.76E-5 YEL045C_d YEL045C_d hom Dubious open reading frame unlikely to encode a protein based on available experimental and comparative sequence data; deletion gives MMS sensitivity, growth defect under alkaline conditions, less than optimal growth upon citric acid stress
0.074 2.06E-5 YDR119W_p VBA4_p hom Protein of unknown function with proposed role as a basic amino acid permease based on phylogeny; GFP-fusion protein localizes to vacuolar membrane; physical interaction with Atg27p suggests a possible role in autophagy; non-essential gene
0.070 4.52E-5 YJL176C SWI3 hom Subunit of the SWI/SNF chromatin remodeling complex; SWI/SNF regulates transcription by remodeling chromosomes; required for transcription of many genes, including ADH1, ADH2, GAL1, HO, INO1 and SUC2; relocates to the cytosol under hypoxic conditions
0.070 5.14E-5 YML025C YML6 het Mitochondrial ribosomal protein of the large subunit, has similarity to E. coli L4 ribosomal protein and human mitoribosomal MRP-L4 protein; essential for viability, unlike most other mitoribosomal proteins
0.069 6.88E-5 YER096W SHC1 hom Sporulation-specific activator of Chs3p (chitin synthase III); required for the synthesis of the chitosan layer of ascospores; transcriptionally induced at alkaline pH; SHC1 has a paralog, SKT5, that arose from the whole genome duplication