YHR065C / RRP3

Protein involved in rRNA processing; required for maturation of the 35S primary transcript of pre-rRNA and for cleavage leading to mature 18S rRNA; homologous to eIF-4a, which is a DEAD box RNA-dependent ATPase with helicase activity

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR065C / RRP3

Click on Significant Values for Screen Details ID:SGTC_141|Compound:0443-0274|FD-Score:-4.46|P-value:2.25E-8 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.69|P-value:2.18E-12 ID:SGTC_335|Compound:4077-0256|FD-Score:2.57|P-value:9.05E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.72|P-value:3.15E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:2.76|P-value:4.04E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-2.52|P-value:9.45E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.82|P-value:1.66E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.77|P-value:3.26E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.72|P-value:3.09E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:2.70|P-value:5.37E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.60|P-value:7.99E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:-2.85|P-value:2.24E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-3.16|P-value:5.13E-5 ID:SGTC_1719|Compound:st032227|FD-Score:-2.96|P-value:1.37E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-2.58|P-value:7.40E-4 ID:SGTC_1804|Compound:st050076|FD-Score:-2.65|P-value:5.47E-4 ID:SGTC_2002|Compound:4023503|FD-Score:2.73|P-value:4.58E-4 ID:SGTC_2159|Compound:5658611|FD-Score:3.75|P-value:2.58E-6 ID:SGTC_2163|Compound:5671233|FD-Score:-2.53|P-value:9.07E-4 ID:SGTC_2315|Compound:7733312|FD-Score:3.81|P-value:1.80E-6 ID:SGTC_2397|Compound:5161689|FD-Score:-3.25|P-value:3.18E-5 ID:SGTC_2413|Compound:inermin|FD-Score:3.55|P-value:7.87E-6 ID:SGTC_2415|Compound:st077333|FD-Score:5.97|P-value:1.74E-13 ID:SGTC_2418|Compound:pentamidine|FD-Score:-2.75|P-value:3.60E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:4.32|P-value:7.30E-8 ID:SGTC_2533|Compound:agelasine|FD-Score:4.36|P-value:5.67E-8 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.34|P-value:2.50E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.14|P-value:2.36E-7 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-2.60|P-value:6.79E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.12|P-value:6.16E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-2.87|P-value:2.05E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.17|P-value:5.85E-5 ID:SGTC_2994|Compound:9053520|FD-Score:2.62|P-value:7.40E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.32|P-value:2.18E-5 ID:SGTC_3129|Compound:9127257|FD-Score:2.85|P-value:2.73E-4 ID:SGTC_3188|Compound:9110789|FD-Score:2.93|P-value:1.92E-4 ID:SGTC_3285|Compound:9112818|FD-Score:-3.24|P-value:3.37E-5 ID:SGTC_141|Compound:0443-0274|FD-Score:-4.46|P-value:2.25E-8 ID:SGTC_275|Compound:trichlorophene|FD-Score:5.69|P-value:2.18E-12 ID:SGTC_335|Compound:4077-0256|FD-Score:2.57|P-value:9.05E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:3.72|P-value:3.15E-6 ID:SGTC_430|Compound:0958-0115|FD-Score:2.76|P-value:4.04E-4 ID:SGTC_867|Compound:0518-0118|FD-Score:-2.52|P-value:9.45E-4 ID:SGTC_995|Compound:3931-2021|FD-Score:3.82|P-value:1.66E-6 ID:SGTC_1049|Compound:3226-0134|FD-Score:-2.77|P-value:3.26E-4 ID:SGTC_1061|Compound:3448-9325|FD-Score:3.72|P-value:3.09E-6 ID:SGTC_1302|Compound:1159-0335|FD-Score:2.70|P-value:5.37E-4 ID:SGTC_1390|Compound:3413-2614|FD-Score:2.60|P-value:7.99E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:-2.85|P-value:2.24E-4 ID:SGTC_1637|Compound:st007924|FD-Score:-3.16|P-value:5.13E-5 ID:SGTC_1719|Compound:st032227|FD-Score:-2.96|P-value:1.37E-4 ID:SGTC_1726|Compound:st035556|FD-Score:-2.58|P-value:7.40E-4 ID:SGTC_1804|Compound:st050076|FD-Score:-2.65|P-value:5.47E-4 ID:SGTC_2002|Compound:4023503|FD-Score:2.73|P-value:4.58E-4 ID:SGTC_2159|Compound:5658611|FD-Score:3.75|P-value:2.58E-6 ID:SGTC_2163|Compound:5671233|FD-Score:-2.53|P-value:9.07E-4 ID:SGTC_2315|Compound:7733312|FD-Score:3.81|P-value:1.80E-6 ID:SGTC_2397|Compound:5161689|FD-Score:-3.25|P-value:3.18E-5 ID:SGTC_2413|Compound:inermin|FD-Score:3.55|P-value:7.87E-6 ID:SGTC_2415|Compound:st077333|FD-Score:5.97|P-value:1.74E-13 ID:SGTC_2418|Compound:pentamidine|FD-Score:-2.75|P-value:3.60E-4 ID:SGTC_2527|Compound:2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone|FD-Score:4.32|P-value:7.30E-8 ID:SGTC_2533|Compound:agelasine|FD-Score:4.36|P-value:5.67E-8 ID:SGTC_2568|Compound:diosmetin|FD-Score:3.34|P-value:2.50E-5 ID:SGTC_2571|Compound:chrysanthemic acid|FD-Score:4.14|P-value:2.36E-7 ID:SGTC_2573|Compound:2',4'-dihydroxychalcone|FD-Score:-2.60|P-value:6.79E-4 ID:SGTC_2575|Compound:plumbagin|FD-Score:-3.12|P-value:6.16E-5 ID:SGTC_2775|Compound:cyproheptadine|FD-Score:-2.87|P-value:2.05E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.17|P-value:5.85E-5 ID:SGTC_2994|Compound:9053520|FD-Score:2.62|P-value:7.40E-4 ID:SGTC_3028|Compound:9087778|FD-Score:-3.32|P-value:2.18E-5 ID:SGTC_3129|Compound:9127257|FD-Score:2.85|P-value:2.73E-4 ID:SGTC_3188|Compound:9110789|FD-Score:2.93|P-value:1.92E-4 ID:SGTC_3285|Compound:9112818|FD-Score:-3.24|P-value:3.37E-5

Top fitness defect scores for YHR065C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2415 st077333 5.97 57.7 µM 1.74E-13 3 1.17E-9 0.36
2 SGTC_275 trichlorophene 5.69 5.7 µM 2.18E-12 mitochondrial stress 1 6.49E-9 0.66
3 SGTC_2533 agelasine 4.36 13.7 µM 5.67E-8 ERG2 9 6.51E-6 0.05
4 SGTC_2527 2',3'-dihydroxy-4-methoxy-4'-ethoxybenzophenone 4.32 63.6 µM 7.30E-8 7 7.74E-6 0.15
5 SGTC_2571 chrysanthemic acid 4.14 100.0 µM 2.36E-7 4 1.72E-5 0.04
6 SGTC_995 3931-2021 3.82 34.6 µM 1.66E-6 RPP1 & pyrimidine depletion 6 6.56E-5 0.02
7 SGTC_2315 7733312 3.81 200.0 µM 1.80E-6 9 6.91E-5 0.16
8 SGTC_2159 5658611 3.75 73.8 µM 2.58E-6 9 8.86E-5 0.17
9 SGTC_1061 3448-9325 3.72 110.0 µM 3.09E-6 7 1.00E-4 0.10
10 SGTC_361 000s-0510 3.72 1.4 µM 3.15E-6 7 1.01E-4 0.34
11 SGTC_2413 inermin 3.55 26.1 µM 7.87E-6 24 1.90E-4 0.09
12 SGTC_2568 diosmetin 3.34 100.0 µM 2.50E-5 18 4.21E-4 0.04
13 SGTC_2837 9008476 3.17 45.5 µM 5.85E-5 13 7.57E-4 0.01
14 SGTC_3188 9110789 2.93 49.5 µM 1.92E-4 iron homeostasis 23 0.00172 0.03
15 SGTC_3129 9127257 2.85 49.5 µM 2.73E-4 19 0.00219 0.18
16 SGTC_430 0958-0115 2.76 16.4 µM 4.04E-4 32 0.00288 0.01
17 SGTC_2002 4023503 2.73 131.0 µM 4.58E-4 mitochondrial processes 32 0.00314 0.03
18 SGTC_1302 1159-0335 2.70 255.0 µM 5.37E-4 17 0.00351 0.13
19 SGTC_2994 9053520 2.62 71.4 µM 7.40E-4 RPP1 & pyrimidine depletion 23 0.00439 0.00
20 SGTC_1390 3413-2614 2.60 145.0 µM 7.99E-4 fatty acid desaturase (OLE1) 34 0.00463 0.10

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.170 4.23E-23 YER028C MIG3 hom Transcriptional regulator; partially nonfunctional in S288C strains but has a major role in catabolite repression and ethanol response in some other strains; involved in response to toxic agents; phosphorylation by Snf1p or the Mec1p pathway inactivates Mig3p, allowing induction of damage response genes; MIG3 has a paralog, MIG2, that arose from the whole genome duplication
0.169 7.21E-23 YER013W PRP22 het DEAH-box RNA-dependent ATPase/ATP-dependent RNA helicase, associates with lariat intermediates before the second catalytic step of splicing; mediates ATP-dependent mRNA release from the spliceosome and unwinds RNA duplexes
0.155 1.41E-19 YFL038C YPT1 het Rab family GTPase, involved in the ER-to-Golgi step of the secretory pathway; complex formation with the Rab escort protein Mrs6p is required for prenylation of Ypt1p by protein geranylgeranyltransferase type II (Bet2p-Bet4p)
0.148 5.31E-18 YBR207W FTH1 hom Putative high affinity iron transporter; involved in transport of intravacuolar stores of iron; forms complex with Fet5p; expression is regulated by iron; proposed to play indirect role in endocytosis; protein abundance increases in response to DNA replication stress
0.144 4.21E-17 YFR019W FAB1 hom 1-phosphatidylinositol-3-phosphate 5-kinase; vacuolar membrane kinase that generates phosphatidylinositol (3,5)P2, which is involved in vacuolar sorting and homeostasis
0.137 1.47E-15 YDL174C DLD1 hom D-lactate dehydrogenase, oxidizes D-lactate to pyruvate, transcription is heme-dependent, repressed by glucose, and derepressed in ethanol or lactate; located in the mitochondrial inner membrane
0.134 5.35E-15 YJR116W_p TDA4_p hom Putative protein of unknown function; null mutant is sensitive to expression of the top1-T722A allele
0.134 6.05E-15 YFL036W RPO41 hom Mitochondrial RNA polymerase; single subunit enzyme similar to those of T3 and T7 bacteriophages; requires a specificity subunit encoded by MTF1 for promoter recognition; Mtf1p interacts with and stabilizes the Rpo41p-promoter complex, enhancing DNA bending and melting to facilitate pre-initiation open complex formation
0.131 2.29E-14 YKL044W_p YKL044W_p hom Protein of unknown function; mRNA identified as translated by ribosome profiling data
0.129 7.22E-14 YGR189C CRH1 hom Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress
0.126 2.91E-13 YOR062C_p YOR062C_p hom Protein of unknown function; similar to YKR075Cp and Reg1p; expression regulated by glucose and Rgt1p; GFP-fusion protein is induced in response to the DNA-damaging agent MMS
0.122 1.30E-12 YKR032W_d YKR032W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.121 2.06E-12 YMR112C MED11 het Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential protein
0.120 3.40E-12 YIL078W THS1 het Threonyl-tRNA synthetase, essential cytoplasmic protein
0.118 6.17E-12 YLR117C CLF1 het Member of the NineTeen Complex (NTC) that contains Prp19p and stabilizes U6 snRNA in catalytic forms of the spliceosome containing U2, U5, and U6 snRNAs; homolog of Drosophila crooked neck protein; interacts with U1 snRNP proteins