YHR070W / TRM5

tRNA(m(1)G37)methyltransferase, methylates a tRNA base adjacent to the anticodon that has a role in prevention of frameshifting; highly conserved across Archaea, Bacteria, and Eukarya

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR070W / TRM5

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:-3.57|P-value:2.24E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.36|P-value:1.64E-10 ID:SGTC_71|Compound:0417-1750|FD-Score:3.25|P-value:6.45E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.95|P-value:4.71E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:3.30|P-value:5.48E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.19|P-value:7.78E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.68|P-value:1.79E-6 ID:SGTC_519|Compound:k048-0011|FD-Score:3.30|P-value:5.41E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.29|P-value:1.10E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:3.45|P-value:3.17E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:7.78|P-value:7.58E-15 ID:SGTC_800|Compound:3448-7362|FD-Score:3.45|P-value:3.21E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.71|P-value:1.20E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.50|P-value:2.85E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.21|P-value:1.52E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.82|P-value:8.51E-5 ID:SGTC_1038|Compound:k292-0204|FD-Score:4.30|P-value:1.04E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.51|P-value:2.56E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.43|P-value:3.43E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.40|P-value:3.86E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.43|P-value:3.43E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.41|P-value:3.70E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:-3.78|P-value:9.77E-5 ID:SGTC_1814|Compound:st052110|FD-Score:3.15|P-value:8.99E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.80|P-value:4.87E-9 ID:SGTC_1942|Compound:st076330|FD-Score:-4.12|P-value:2.46E-5 ID:SGTC_1961|Compound:st076550|FD-Score:3.23|P-value:6.97E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.41|P-value:3.66E-4 ID:SGTC_2243|Compound:6634756|FD-Score:6.39|P-value:1.33E-10 ID:SGTC_2292|Compound:7671323|FD-Score:4.79|P-value:1.10E-6 ID:SGTC_2344|Compound:7990960|FD-Score:-4.17|P-value:1.98E-5 ID:SGTC_2349|Compound:9023730|FD-Score:-3.69|P-value:1.42E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:4.44|P-value:5.58E-6 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.35|P-value:4.57E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.24|P-value:6.76E-4 ID:SGTC_2623|Compound:humulene|FD-Score:5.43|P-value:3.95E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.59|P-value:3.67E-11 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.84|P-value:7.81E-5 ID:SGTC_2699|Compound:st077704|FD-Score:3.42|P-value:3.59E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.61|P-value:1.77E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.57|P-value:2.05E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.73|P-value:1.13E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.63|P-value:1.61E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.21|P-value:7.38E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.01|P-value:3.65E-5 ID:SGTC_2810|Compound:7959871|FD-Score:4.42|P-value:6.09E-6 ID:SGTC_3062|Compound:9077494|FD-Score:-4.72|P-value:1.68E-6 ID:SGTC_3082|Compound:9117008|FD-Score:-3.61|P-value:1.92E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.35|P-value:4.48E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.39|P-value:4.24E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.17|P-value:8.46E-4 ID:SGTC_49|Compound:0717-0965|FD-Score:-3.57|P-value:2.24E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.36|P-value:1.64E-10 ID:SGTC_71|Compound:0417-1750|FD-Score:3.25|P-value:6.45E-4 ID:SGTC_145|Compound:3737-0113|FD-Score:3.95|P-value:4.71E-5 ID:SGTC_181|Compound:1447-1644|FD-Score:3.30|P-value:5.48E-4 ID:SGTC_237|Compound:nikkomycin|FD-Score:3.46|P-value:3.03E-4 ID:SGTC_309|Compound:k408-0051|FD-Score:3.19|P-value:7.78E-4 ID:SGTC_354|Compound:2783-3500|FD-Score:4.68|P-value:1.79E-6 ID:SGTC_519|Compound:k048-0011|FD-Score:3.30|P-value:5.41E-4 ID:SGTC_539|Compound:4859-0462|FD-Score:4.29|P-value:1.10E-5 ID:SGTC_586|Compound:0957-0265|FD-Score:3.45|P-value:3.17E-4 ID:SGTC_682|Compound:0kpi-0099|FD-Score:7.78|P-value:7.58E-15 ID:SGTC_800|Compound:3448-7362|FD-Score:3.45|P-value:3.21E-4 ID:SGTC_933|Compound:2914-0560|FD-Score:3.71|P-value:1.20E-4 ID:SGTC_1003|Compound:4049-0218|FD-Score:-3.50|P-value:2.85E-4 ID:SGTC_1034|Compound:k072-0165|FD-Score:4.21|P-value:1.52E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-3.82|P-value:8.51E-5 ID:SGTC_1038|Compound:k292-0204|FD-Score:4.30|P-value:1.04E-5 ID:SGTC_1047|Compound:2043-3044|FD-Score:3.51|P-value:2.56E-4 ID:SGTC_1186|Compound:1319-0216|FD-Score:3.43|P-value:3.43E-4 ID:SGTC_1274|Compound:0865-0114|FD-Score:3.40|P-value:3.86E-4 ID:SGTC_1451|Compound:4152-0210|FD-Score:3.43|P-value:3.43E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:3.41|P-value:3.70E-4 ID:SGTC_1490|Compound:4089-0339|FD-Score:-3.78|P-value:9.77E-5 ID:SGTC_1814|Compound:st052110|FD-Score:3.15|P-value:8.99E-4 ID:SGTC_1844|Compound:rapamycin|FD-Score:5.80|P-value:4.87E-9 ID:SGTC_1942|Compound:st076330|FD-Score:-4.12|P-value:2.46E-5 ID:SGTC_1961|Compound:st076550|FD-Score:3.23|P-value:6.97E-4 ID:SGTC_2156|Compound:5649887|FD-Score:3.41|P-value:3.66E-4 ID:SGTC_2243|Compound:6634756|FD-Score:6.39|P-value:1.33E-10 ID:SGTC_2292|Compound:7671323|FD-Score:4.79|P-value:1.10E-6 ID:SGTC_2344|Compound:7990960|FD-Score:-4.17|P-value:1.98E-5 ID:SGTC_2349|Compound:9023730|FD-Score:-3.69|P-value:1.42E-4 ID:SGTC_2509|Compound:methyl 7-deshydroxypyrogallin-4-carboxylate|FD-Score:4.44|P-value:5.58E-6 ID:SGTC_2589|Compound:lobaric acid|FD-Score:3.35|P-value:4.57E-4 ID:SGTC_2616|Compound:abietic acid|FD-Score:3.24|P-value:6.76E-4 ID:SGTC_2623|Compound:humulene|FD-Score:5.43|P-value:3.95E-8 ID:SGTC_2679|Compound:rapamycin|FD-Score:6.59|P-value:3.67E-11 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:-3.84|P-value:7.81E-5 ID:SGTC_2699|Compound:st077704|FD-Score:3.42|P-value:3.59E-4 ID:SGTC_2711|Compound:st079103|FD-Score:3.61|P-value:1.77E-4 ID:SGTC_2713|Compound:st079149|FD-Score:3.57|P-value:2.05E-4 ID:SGTC_2749|Compound:bexarotene|FD-Score:3.73|P-value:1.13E-4 ID:SGTC_2756|Compound:carbadox|FD-Score:3.63|P-value:1.61E-4 ID:SGTC_2778|Compound:tioconazole|FD-Score:3.21|P-value:7.38E-4 ID:SGTC_2788|Compound:5282734|FD-Score:4.01|P-value:3.65E-5 ID:SGTC_2810|Compound:7959871|FD-Score:4.42|P-value:6.09E-6 ID:SGTC_3062|Compound:9077494|FD-Score:-4.72|P-value:1.68E-6 ID:SGTC_3082|Compound:9117008|FD-Score:-3.61|P-value:1.92E-4 ID:SGTC_3152|Compound:9099253|FD-Score:3.35|P-value:4.48E-4 ID:SGTC_3301|Compound:9125354|FD-Score:-3.39|P-value:4.24E-4 ID:SGTC_3310|Compound:9130229|FD-Score:3.17|P-value:8.46E-4

Top fitness defect scores for YHR070W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_682 0kpi-0099 7.78 17.0 µM 7.58E-15 9 3.76E-15 0.13
2 SGTC_2679 rapamycin 6.59 1.0 nM 3.67E-11 24 2.23E-11 0.12
3 SGTC_2243 6634756 6.39 200.0 µM 1.33E-10 2 8.33E-11 2.10
4 SGTC_58 0957-0393 6.36 9.0 µM 1.64E-10 cell wall 26 1.04E-10 0.16
5 SGTC_1844 rapamycin 5.80 4.0 nM 4.87E-9 23 3.33E-9 0.01
6 SGTC_2623 humulene 5.43 100.0 µM 3.95E-8 1 2.84E-8 1.75
7 SGTC_2292 7671323 4.79 168.1 µM 1.10E-6 RNA processing & uracil transport 7 8.54E-7 0.09
8 SGTC_354 2783-3500 4.68 4.9 µM 1.79E-6 cell wall 22 1.41E-6 0.29
9 SGTC_2509 methyl 7-deshydroxypyrogallin-4-carboxylate 4.44 54.6 µM 5.58E-6 RPP1 & pyrimidine depletion 7 4.49E-6 0.04
10 SGTC_2810 7959871 4.42 45.5 µM 6.09E-6 copper-dependent oxidative stress 13 4.92E-6 0.06
11 SGTC_1038 k292-0204 4.30 154.0 µM 1.04E-5 4 8.46E-6 0.35
12 SGTC_539 4859-0462 4.29 2.5 µM 1.10E-5 RNA pol III & RNase P/MRP 18 9.00E-6 0.12
13 SGTC_1034 k072-0165 4.21 12.7 µM 1.52E-5 Golgi 5 1.25E-5 0.34
14 SGTC_2788 5282734 4.01 71.4 µM 3.65E-5 7 3.07E-5 0.03
15 SGTC_145 3737-0113 3.95 38.4 µM 4.71E-5 DNA intercalators 50 3.99E-5 0.03
16 SGTC_2749 bexarotene 3.73 57.4 µM 1.13E-4 4 9.73E-5 0.01
17 SGTC_933 2914-0560 3.71 114.0 µM 1.20E-4 8 1.03E-4 0.13
18 SGTC_2756 carbadox 3.63 75.1 µM 1.61E-4 26 1.40E-4 0.02
19 SGTC_2711 st079103 3.61 59.1 µM 1.77E-4 3 1.54E-4 0.39
20 SGTC_2713 st079149 3.57 53.4 µM 2.05E-4 6 1.79E-4 0.01

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.237 5.94E-44 YKL203C TOR2 het PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that regulates growth in response to nutrients and TORC2, a complex that regulates cell-cycle dependent polarization of the actin cytoskeleton; involved in meiosis; TOR2 has a paralog, TOR1, that arose from the whole genome duplication
0.219 1.32E-37 YML121W GTR1 hom Cytoplasmic GTP binding protein and negative regulator of the Ran/Tc4 GTPase cycle; component of GSE complex, which is required for sorting of Gap1p; involved in phosphate transport and telomeric silencing; similar to human RagA and RagB
0.212 1.75E-35 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.212 2.03E-35 YML126C ERG13 het 3-hydroxy-3-methylglutaryl-CoA (HMG-CoA) synthase, catalyzes the formation of HMG-CoA from acetyl-CoA and acetoacetyl-CoA; involved in the second step in mevalonate biosynthesis
0.200 2.06E-31 YHR186C KOG1 het Subunit of TORC1, a rapamycin-sensitive complex involved in growth control that contains Tor1p or Tor2p, Lst8p and Tco89p; contains four HEAT repeats and seven WD-40 repeats; may act as a scaffold protein to couple TOR and its effectors
0.194 1.23E-29 YLR114C AVL9 hom Conserved protein involved in exocytic transport from the Golgi; mutation is synthetically lethal with apl2 vps1 double mutation; member of a protein superfamily with orthologs in diverse organisms; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.186 1.78E-27 YJR066W TOR1 hom PIK-related protein kinase and rapamycin target; subunit of TORC1, a complex that controls growth in response to nutrients by regulating translation, transcription, ribosome biogenesis, nutrient transport and autophagy; involved in meiosis; TOR1 has a paralog, TOR2, that arose from the whole genome duplication
0.178 2.89E-25 YBR077C SLM4 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; gene exhibits synthetic genetic interaction with MSS4
0.169 6.00E-23 YDR023W SES1 het Cytosolic seryl-tRNA synthetase, class II aminoacyl-tRNA synthetase that aminoacylates tRNA(Ser), displays tRNA-dependent amino acid recognition which enhances discrimination of the serine substrate, interacts with peroxin Pex21p
0.167 1.82E-22 YGL211W NCS6 hom Protein required for thiolation of the uridine at the wobble position of Gln, Lys, and Glu tRNAs; has a role in urmylation and in invasive and pseudohyphal growth; inhibits replication of Brome mosaic virus in S. cerevisiae
0.167 2.53E-22 YKL110C KTI12 hom Protein that plays a role, with Elongator complex, in modification of wobble nucleosides in tRNA; involved in sensitivity to G1 arrest induced by zymocin; interacts with chromatin throughout the genome; also interacts with Cdc19p
0.164 1.32E-21 YJL069C UTP18 het Possible U3 snoRNP protein involved in maturation of pre-18S rRNA, based on computational analysis of large-scale protein-protein interaction data
0.163 1.88E-21 YLR384C IKI3 hom Subunit of Elongator complex, which is required for modification of wobble nucleosides in tRNA; maintains structural integrity of Elongator; homolog of human IKAP, mutations in which cause familial dysautonomia (FD)
0.162 4.02E-21 YKR007W MEH1 hom Component of the EGO complex, which is involved in the regulation of microautophagy, and of the GSE complex, which is required for proper sorting of amino acid permease Gap1p; loss results in a defect in vacuolar acidification
0.155 1.59E-19 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2