YHR074W / QNS1

Glutamine-dependent NAD(+) synthetase, essential for the formation of NAD(+) from nicotinic acid adenine dinucleotide

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR074W / QNS1

Click on Significant Values for Screen Details ID:SGTC_142|Compound:0335-0849|FD-Score:3.46|P-value:2.89E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:3.99|P-value:3.61E-5 ID:SGTC_229|Compound:benomyl|FD-Score:5.85|P-value:2.94E-9 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.16|P-value:8.32E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.17|P-value:8.07E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:-3.32|P-value:4.65E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:-3.13|P-value:9.15E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.37|P-value:3.96E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.47|P-value:2.71E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:3.38|P-value:3.80E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.56|P-value:1.96E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.30|P-value:5.05E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.14|P-value:8.89E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.72|P-value:1.06E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:4.10|P-value:2.22E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:5.60|P-value:1.21E-8 ID:SGTC_812|Compound:4251-0442|FD-Score:3.86|P-value:6.20E-5 ID:SGTC_887|Compound:0885-0007|FD-Score:3.23|P-value:6.61E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:3.33|P-value:4.55E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.30|P-value:5.06E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.35|P-value:4.29E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.77|P-value:8.77E-5 ID:SGTC_1070|Compound:indatraline|FD-Score:3.20|P-value:7.28E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.69|P-value:1.48E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.85|P-value:6.33E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:5.76|P-value:4.92E-9 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.47|P-value:2.71E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:-3.38|P-value:3.85E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:8.05|P-value:5.38E-16 ID:SGTC_1274|Compound:0865-0114|FD-Score:-3.48|P-value:2.62E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:-3.33|P-value:4.52E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:-4.25|P-value:1.17E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:3.75|P-value:9.30E-5 ID:SGTC_1697|Compound:st025554|FD-Score:3.28|P-value:5.55E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.30|P-value:5.03E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.15|P-value:8.65E-4 ID:SGTC_1815|Compound:st051655|FD-Score:4.68|P-value:1.60E-6 ID:SGTC_1825|Compound:st052143|FD-Score:3.76|P-value:9.25E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.13|P-value:9.09E-4 ID:SGTC_2431|Compound:5490079|FD-Score:4.16|P-value:1.76E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-3.36|P-value:4.04E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.45|P-value:2.97E-4 ID:SGTC_2910|Compound:7948405|FD-Score:4.99|P-value:3.33E-7 ID:SGTC_2985|Compound:9022375|FD-Score:3.19|P-value:7.49E-4 ID:SGTC_3024|Compound:9090631|FD-Score:4.04|P-value:2.90E-5 ID:SGTC_3190|Compound:9110366|FD-Score:5.62|P-value:1.13E-8 ID:SGTC_3199|Compound:9111318|FD-Score:3.32|P-value:4.72E-4 ID:SGTC_3289|Compound:9119912|FD-Score:4.75|P-value:1.12E-6 ID:SGTC_142|Compound:0335-0849|FD-Score:3.46|P-value:2.89E-4 ID:SGTC_225|Compound:nsc-122657|FD-Score:3.99|P-value:3.61E-5 ID:SGTC_229|Compound:benomyl|FD-Score:5.85|P-value:2.94E-9 ID:SGTC_233|Compound:rapamycin|FD-Score:-3.16|P-value:8.32E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.17|P-value:8.07E-4 ID:SGTC_300|Compound:r073-0011|FD-Score:-3.32|P-value:4.65E-4 ID:SGTC_375|Compound:3970-0961|FD-Score:-3.13|P-value:9.15E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-3.37|P-value:3.96E-4 ID:SGTC_425|Compound:3825-7017|FD-Score:3.47|P-value:2.71E-4 ID:SGTC_427|Compound:1354-0053|FD-Score:3.38|P-value:3.80E-4 ID:SGTC_430|Compound:0958-0115|FD-Score:-3.56|P-value:1.96E-4 ID:SGTC_538|Compound:2922-0838|FD-Score:3.30|P-value:5.05E-4 ID:SGTC_635|Compound:k038-0028|FD-Score:3.14|P-value:8.89E-4 ID:SGTC_641|Compound:4092-0595|FD-Score:3.72|P-value:1.06E-4 ID:SGTC_734|Compound:2023-0417|FD-Score:4.10|P-value:2.22E-5 ID:SGTC_794|Compound:k038-0031|FD-Score:5.60|P-value:1.21E-8 ID:SGTC_812|Compound:4251-0442|FD-Score:3.86|P-value:6.20E-5 ID:SGTC_887|Compound:0885-0007|FD-Score:3.23|P-value:6.61E-4 ID:SGTC_937|Compound:3122-0005|FD-Score:3.33|P-value:4.55E-4 ID:SGTC_958|Compound:1222-0007|FD-Score:3.30|P-value:5.06E-4 ID:SGTC_1053|Compound:3366-3204|FD-Score:3.35|P-value:4.29E-4 ID:SGTC_1062|Compound:mesoridazine|FD-Score:3.77|P-value:8.77E-5 ID:SGTC_1070|Compound:indatraline|FD-Score:3.20|P-value:7.28E-4 ID:SGTC_1087|Compound:pterostilbene|FD-Score:4.69|P-value:1.48E-6 ID:SGTC_1197|Compound:1601-0112|FD-Score:-3.85|P-value:6.33E-5 ID:SGTC_1205|Compound:4161-2785|FD-Score:5.76|P-value:4.92E-9 ID:SGTC_1252|Compound:0493-0875|FD-Score:-3.47|P-value:2.71E-4 ID:SGTC_1267|Compound:0828-0272|FD-Score:-3.38|P-value:3.85E-4 ID:SGTC_1271|Compound:0832-2808|FD-Score:8.05|P-value:5.38E-16 ID:SGTC_1274|Compound:0865-0114|FD-Score:-3.48|P-value:2.62E-4 ID:SGTC_1276|Compound:0871-0068|FD-Score:-3.33|P-value:4.52E-4 ID:SGTC_1287|Compound:0971-0001|FD-Score:-4.25|P-value:1.17E-5 ID:SGTC_1390|Compound:3413-2614|FD-Score:3.75|P-value:9.30E-5 ID:SGTC_1697|Compound:st025554|FD-Score:3.28|P-value:5.55E-4 ID:SGTC_1733|Compound:st037357|FD-Score:3.30|P-value:5.03E-4 ID:SGTC_1737|Compound:st035557|FD-Score:-3.15|P-value:8.65E-4 ID:SGTC_1815|Compound:st051655|FD-Score:4.68|P-value:1.60E-6 ID:SGTC_1825|Compound:st052143|FD-Score:3.76|P-value:9.25E-5 ID:SGTC_2135|Compound:5326629|FD-Score:-3.13|P-value:9.09E-4 ID:SGTC_2431|Compound:5490079|FD-Score:4.16|P-value:1.76E-5 ID:SGTC_2482|Compound:5981709|FD-Score:-3.36|P-value:4.04E-4 ID:SGTC_2682|Compound:pyrogallin|FD-Score:-3.45|P-value:2.97E-4 ID:SGTC_2910|Compound:7948405|FD-Score:4.99|P-value:3.33E-7 ID:SGTC_2985|Compound:9022375|FD-Score:3.19|P-value:7.49E-4 ID:SGTC_3024|Compound:9090631|FD-Score:4.04|P-value:2.90E-5 ID:SGTC_3190|Compound:9110366|FD-Score:5.62|P-value:1.13E-8 ID:SGTC_3199|Compound:9111318|FD-Score:3.32|P-value:4.72E-4 ID:SGTC_3289|Compound:9119912|FD-Score:4.75|P-value:1.12E-6

Top fitness defect scores for YHR074W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_1271 0832-2808 8.05 169.0 µM 5.38E-16 1 4.01E-16 1.70
2 SGTC_229 benomyl 5.85 22.9 µM 2.94E-9 tubulin folding & SWR complex 10 2.51E-9 0.33
3 SGTC_1205 4161-2785 5.76 76.0 µM 4.92E-9 1 4.22E-9 1.06
4 SGTC_3190 9110366 5.62 49.5 µM 1.13E-8 1 9.76E-9 1.57
5 SGTC_794 k038-0031 5.60 316.0 µM 1.21E-8 ubiquinone biosynthesis & proteasome 7 1.04E-8 0.25
6 SGTC_2910 7948405 4.99 26.0 µM 3.33E-7 plasma membrane duress 2 2.96E-7 0.88
7 SGTC_3289 9119912 4.75 71.4 µM 1.12E-6 1 1.01E-6 0.38
8 SGTC_1087 pterostilbene 4.69 9.1 µM 1.48E-6 2 1.34E-6 0.37
9 SGTC_1815 st051655 4.68 58.2 µM 1.60E-6 TSC3-RPN4 7 1.44E-6 0.08
10 SGTC_2431 5490079 4.16 200.0 µM 1.76E-5 5 1.62E-5 0.06
11 SGTC_734 2023-0417 4.10 80.0 µM 2.22E-5 ergosterol biosynthesis 6 2.05E-5 0.07
12 SGTC_3024 9090631 4.04 49.5 µM 2.90E-5 3 2.68E-5 0.56
13 SGTC_225 nsc-122657 3.99 1.0 µM 3.61E-5 2 3.35E-5 0.17
14 SGTC_812 4251-0442 3.86 148.0 µM 6.20E-5 4 5.77E-5 0.24
15 SGTC_1062 mesoridazine 3.77 14.2 µM 8.77E-5 5 8.18E-5 0.00
16 SGTC_1825 st052143 3.76 62.6 µM 9.25E-5 8 8.64E-5 0.33
17 SGTC_1390 3413-2614 3.75 145.0 µM 9.30E-5 fatty acid desaturase (OLE1) 12 8.69E-5 0.16
18 SGTC_641 4092-0595 3.72 30.0 µM 1.06E-4 11 9.88E-5 0.03
19 SGTC_425 3825-7017 3.47 13.1 µM 2.71E-4 15 2.56E-4 0.05
20 SGTC_142 0335-0849 3.46 62.7 µM 2.89E-4 ubiquinone biosynthesis & proteasome 27 2.73E-4 0.06

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.114 4.20E-11 YBR275C RIF1 hom Protein that binds to the Rap1p C-terminus and acts synergistically with Rif2p to help control telomere length and establish telomeric silencing; deletion results in telomere elongation
0.103 2.37E-9 YPR065W ROX1 hom Heme-dependent repressor of hypoxic genes; mediates aerobic transcriptional repression of hypoxia induced genes such as COX5b and CYC7; repressor function regulated through decreased promoter occupancy in response to oxidative stress; contains an HMG domain that is responsible for DNA bending activity; involved in the hyperosmotic stress resistance
0.100 6.34E-9 YNL090W RHO2 hom Non-essential small GTPase of the Rho/Rac subfamily of Ras-like proteins, involved in the establishment of cell polarity and in microtubule assembly
0.098 1.22E-8 YOR254C SEC63 het Essential subunit of Sec63 complex (Sec63p, Sec62p, Sec66p and Sec72p); with Sec61 complex, Kar2p/BiP and Lhs1p forms a channel competent for SRP-dependent and post-translational SRP-independent protein targeting and import into the ER
0.095 3.86E-8 YIL142W CCT2 het Subunit beta of the cytosolic chaperonin Cct ring complex, related to Tcp1p, required for the assembly of actin and tubulins in vivo
0.090 1.63E-7 YOL008W COQ10 hom Coenzyme Q (ubiquinone) binding protein, functions in the delivery of Q6 to its proper location for electron transport during respiration; START domain protein with homologs in bacteria and eukaryotes
0.088 3.77E-7 YBR231C SWC5 hom Component of the SWR1 complex; complex exchanges histone variant H2AZ (Htz1p) for chromatin-bound histone H2A; protein abundance increases in response to DNA replication stress
0.087 4.45E-7 YDR534C FIT1 hom Mannoprotein that is incorporated into the cell wall via a glycosylphosphatidylinositol (GPI) anchor, involved in the retention of siderophore-iron in the cell wall
0.086 5.98E-7 YOR046C DBP5 het Cytoplasmic ATP-dependent RNA helicase of the DEAD-box family; involved in mRNA export from the nucleus; involved in translation termination; ATP/ADP cycling is regulated by Gle1p and Nup159p
0.083 1.51E-6 YML034W SRC1 hom Inner nuclear membrane protein; functions in regulation of subtelomeric genes and is linked to TREX (transcription export) factors; SRC1 produces 2 splice variant proteins with different functions; alternative splicing of SRC1 pre-mRNA is promoted by Hub1p; mutant has aneuploidy tolerance
0.082 1.86E-6 YJL133W MRS3 hom Iron transporter, mediates Fe2+ transport across inner mito membrane; mitochondrial carrier family member; active under low-iron conditions; may transport other cations; MRS3 has a paralog, MRS4, that arose from the whole genome duplication
0.081 2.32E-6 YER118C SHO1 hom Transmembrane osmosensor involved in activation of the Cdc42p- and MAP kinase-dependent filamentous growth pathway and the high-osmolarity glycerol response pathway; phosphorylated by Hog1p; interacts with Pbs2p, Msb2p, Hkr1p, and Ste11p
0.080 3.39E-6 YGL119W COQ8 hom Protein required for ubiquinone biosynthesis and respiratory growth; exhibits genetic interaction with COQ9, suggesting a common function; similar to prokaryotic proteins involved in early steps of ubiquinone biosynthesis; COQ8 has a paralog, YBR230W-A, that arose from the whole genome duplication
0.080 3.72E-6 YJR129C_p YJR129C_p hom Putative protein of unknown function; predicted S-adenosylmethionine-dependent methyltransferase of the seven beta-strand family; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.079 4.57E-6 YIL090W ICE2 hom Integral ER membrane protein with type-III transmembrane domains; required for maintenance of ER zinc homeostasis; necessary for efficient targeting of Trm1p tRNA methyltransferase to inner nuclear membrane; mutations cause defects in cortical ER morphology in both the mother and daughter cells