YHR083W / SAM35

Essential component of the sorting and assembly machinery (SAM complex or TOB complex) of the mitochondrial outer membrane, which binds precursors of beta-barrel proteins and facilitates their insertion into the outer membrane

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR083W / SAM35

Click on Significant Values for Screen Details ID:SGTC_43|Compound:0570-0105|FD-Score:4.09|P-value:6.64E-5 ID:SGTC_80|Compound:0159-0065|FD-Score:-3.41|P-value:6.04E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:5.13|P-value:7.94E-7 ID:SGTC_125|Compound:0133-0007|FD-Score:4.38|P-value:2.12E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.79|P-value:1.59E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.55|P-value:3.76E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.34|P-value:9.17E-4 ID:SGTC_336|Compound:4111-0029|FD-Score:4.53|P-value:1.15E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.37|P-value:6.87E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:3.78|P-value:2.12E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.73|P-value:2.03E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.16|P-value:4.99E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:3.50|P-value:5.44E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.45|P-value:6.45E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.83|P-value:3.12E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.82|P-value:1.77E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.30|P-value:2.96E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-3.53|P-value:3.99E-4 ID:SGTC_2327|Compound:6189656|FD-Score:-4.50|P-value:1.01E-5 ID:SGTC_2342|Compound:7990637|FD-Score:-3.39|P-value:6.46E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.35|P-value:8.81E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-4.11|P-value:4.87E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.57|P-value:3.56E-4 ID:SGTC_2487|Compound:5918373|FD-Score:-3.61|P-value:3.04E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.51|P-value:5.19E-4 ID:SGTC_2706|Compound:st078239|FD-Score:4.29|P-value:3.07E-5 ID:SGTC_2767|Compound:sibutramine|FD-Score:-4.23|P-value:3.09E-5 ID:SGTC_2806|Compound:7952793|FD-Score:3.47|P-value:6.12E-4 ID:SGTC_2978|Compound:9011784|FD-Score:4.14|P-value:5.45E-5 ID:SGTC_2981|Compound:9013952|FD-Score:5.00|P-value:1.47E-6 ID:SGTC_2994|Compound:9053520|FD-Score:3.56|P-value:4.43E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-5.63|P-value:5.04E-8 ID:SGTC_3315|Compound:9136395|FD-Score:-4.72|P-value:3.93E-6 ID:SGTC_43|Compound:0570-0105|FD-Score:4.09|P-value:6.64E-5 ID:SGTC_80|Compound:0159-0065|FD-Score:-3.41|P-value:6.04E-4 ID:SGTC_103|Compound:0844-0104|FD-Score:5.13|P-value:7.94E-7 ID:SGTC_125|Compound:0133-0007|FD-Score:4.38|P-value:2.12E-5 ID:SGTC_160|Compound:3453-0671|FD-Score:-3.79|P-value:1.59E-4 ID:SGTC_310|Compound:1273-0058|FD-Score:-3.55|P-value:3.76E-4 ID:SGTC_334|Compound:1502-1024|FD-Score:3.34|P-value:9.17E-4 ID:SGTC_336|Compound:4111-0029|FD-Score:4.53|P-value:1.15E-5 ID:SGTC_611|Compound:3453-1652|FD-Score:-3.37|P-value:6.87E-4 ID:SGTC_684|Compound:1140-0066|FD-Score:3.78|P-value:2.12E-4 ID:SGTC_1134|Compound:3477-0009|FD-Score:-3.73|P-value:2.03E-4 ID:SGTC_1161|Compound:k007-0610|FD-Score:4.16|P-value:4.99E-5 ID:SGTC_1372|Compound:2752-0194|FD-Score:3.50|P-value:5.44E-4 ID:SGTC_1515|Compound:3460-0031|FD-Score:3.45|P-value:6.45E-4 ID:SGTC_1629|Compound:st005085|FD-Score:4.83|P-value:3.12E-6 ID:SGTC_1631|Compound:benzylvanillin|FD-Score:3.82|P-value:1.77E-4 ID:SGTC_1979|Compound:st071809|FD-Score:4.30|P-value:2.96E-5 ID:SGTC_2073|Compound:5212005|FD-Score:-3.53|P-value:3.99E-4 ID:SGTC_2327|Compound:6189656|FD-Score:-4.50|P-value:1.01E-5 ID:SGTC_2342|Compound:7990637|FD-Score:-3.39|P-value:6.46E-4 ID:SGTC_2385|Compound:9074692|FD-Score:3.35|P-value:8.81E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:-4.11|P-value:4.87E-5 ID:SGTC_2436|Compound:5633522|FD-Score:-3.57|P-value:3.56E-4 ID:SGTC_2487|Compound:5918373|FD-Score:-3.61|P-value:3.04E-4 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.51|P-value:5.19E-4 ID:SGTC_2706|Compound:st078239|FD-Score:4.29|P-value:3.07E-5 ID:SGTC_2767|Compound:sibutramine|FD-Score:-4.23|P-value:3.09E-5 ID:SGTC_2806|Compound:7952793|FD-Score:3.47|P-value:6.12E-4 ID:SGTC_2978|Compound:9011784|FD-Score:4.14|P-value:5.45E-5 ID:SGTC_2981|Compound:9013952|FD-Score:5.00|P-value:1.47E-6 ID:SGTC_2994|Compound:9053520|FD-Score:3.56|P-value:4.43E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-5.63|P-value:5.04E-8 ID:SGTC_3315|Compound:9136395|FD-Score:-4.72|P-value:3.93E-6

Top fitness defect scores for YHR083W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_103 0844-0104 5.13 51.8 µM 7.94E-7 ERAD & cell cycle 35 1.45E-7 0.07
2 SGTC_2981 9013952 5.00 39.0 µM 1.47E-6 2 2.92E-7 0.19
3 SGTC_1629 st005085 4.83 44.9 µM 3.12E-6 RPP1 & pyrimidine depletion 11 6.83E-7 0.64
4 SGTC_336 4111-0029 4.53 111.0 µM 1.15E-5 1 2.97E-6 0.80
5 SGTC_125 0133-0007 4.38 11.8 µM 2.12E-5 DNA intercalators 5 5.95E-6 0.63
6 SGTC_1979 st071809 4.30 45.3 µM 2.96E-5 RPP1 & pyrimidine depletion 6 8.65E-6 0.20
7 SGTC_2706 st078239 4.29 82.5 µM 3.07E-5 2 9.03E-6 0.45
8 SGTC_1161 k007-0610 4.16 110.0 µM 4.99E-5 ERAD & cell cycle 40 1.56E-5 0.11
9 SGTC_2978 9011784 4.14 43.0 µM 5.45E-5 4 1.73E-5 0.19
10 SGTC_43 0570-0105 4.09 72.5 µM 6.64E-5 ERG2 8 2.16E-5 0.03
11 SGTC_1631 benzylvanillin 3.82 81.9 µM 1.77E-4 Golgi 7 6.55E-5 0.12
12 SGTC_684 1140-0066 3.78 328.0 µM 2.12E-4 Golgi 5 7.99E-5 0.07
13 SGTC_2994 9053520 3.56 71.4 µM 4.43E-4 RPP1 & pyrimidine depletion 13 1.84E-4 0.06
14 SGTC_2501 sodium tetradecyl sulfate 3.51 13.4 µM 5.19E-4 7 2.20E-4 0.19
15 SGTC_1372 2752-0194 3.50 270.0 µM 5.44E-4 3 2.32E-4 0.08
16 SGTC_2806 7952793 3.47 58.4 µM 6.12E-4 10 2.65E-4 0.21
17 SGTC_1515 3460-0031 3.45 42.1 µM 6.45E-4 DNA intercalators 11 2.81E-4 0.09
18 SGTC_2385 9074692 3.35 200.0 µM 8.81E-4 10 4.00E-4 0.04
19 SGTC_334 1502-1024 3.34 68.5 µM 9.17E-4 4 4.18E-4 0.02
20 SGTC_1150 3909-7983 3.30 10.9 µM 0.00103 14 4.78E-4 9.11E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.196 1.58E-30 YDL148C NOP14 het Nucleolar protein, forms a complex with Noc4p that mediates maturation and nuclear export of 40S ribosomal subunits; also present in the small subunit processome complex, which is required for processing of pre-18S rRNA
0.142 1.34E-16 YOL065C INP54 hom Phosphatidylinositol 4,5-bisphosphate 5-phosphatase with a role in secretion, localizes to the endoplasmic reticulum via the C-terminal tail; lacks the Sac1 domain and proline-rich region found in the other 3 INP proteins
0.124 4.81E-13 YOR363C PIP2 hom Autoregulatory oleate-specific transcriptional activator of peroxisome proliferation, contains Zn(2)-Cys(6) cluster domain, forms heterodimer with Oaf1p, binds oleate response elements (OREs), activates beta-oxidation genes; PIP2 has a paralog, OAF1, that arose from the whole genome duplication
0.123 8.55E-13 YDR186C YDR186C hom Putative protein of unknown function; may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.122 1.26E-12 YDL190C UFD2 hom Ubiquitin chain assembly factor (E4) that cooperates with a ubiquitin-activating enzyme (E1), a ubiquitin-conjugating enzyme (E2), and a ubiquitin protein ligase (E3) to conjugate ubiquitin to substrates; also functions as an E3
0.118 7.79E-12 YDL126C CDC48 het AAA ATPase involved in multiple processes; subunit of polyubiquitin-selective segregase complex involved in ERAD, cell wall integrity during heat stress, mitotic spindle disassembly; subunit of complex involved in mitochondria-associated degradation; role in mobilizing membrane bound transcription factors by regulated ubiquitin/proteasome-dependent processing; roles in macroautophagy, PMN, RAD, ribophagy, and homotypic ER membrane fusion; functional ortholog of human p97/VCP
0.115 2.21E-11 YOR246C ENV9 hom Protein proposed to be involved in vacuolar functions; mutant shows defect in CPY processing and defects in vacuolar morphology; has similarity to oxidoreductases, found in lipid particles; required for replication of Brome mosaic virus in S. cerevisiae, a model system for studying replication of positive-strand RNA viruses in their natural hosts
0.112 9.07E-11 YPL010W RET3 het Zeta subunit of the coatomer complex (COPI), which coats Golgi-derived transport vesicles; involved in retrograde transport between Golgi and ER
0.110 2.05E-10 YER041W YEN1 hom Holliday junction resolvase; localization is cell-cycle dependent and regulated by Cdc28p phosphorylation; homolog of human GEN1 and has similarity to S. cerevisiae endonuclease Rth1p
0.108 3.65E-10 YML105C SEC65 het Subunit of the signal recognition particle (SRP), involved in protein targeting to the ER; interacts with Srp54p; homolog of mammalian SRP19
0.107 4.98E-10 YDL122W UBP1 hom Ubiquitin-specific protease that removes ubiquitin from ubiquitinated proteins; cleaves at the C terminus of ubiquitin fusions irrespective of their size; capable of cleaving polyubiquitin chains
0.107 4.88E-10 YER084W_p YER084W_p hom Protein of unknown function; expressed at both mRNA and protein levels
0.104 1.43E-9 YFR008W FAR7 hom Protein involved in recovery from pheromone-induced cell cycle arrest; acts in a Far1p-independent pathway; interacts with Far3p, Far8p, Far9p, Far10p, and Far11p; protein abundance increases in response to DNA replication stress
0.104 1.56E-9 YMR029C FAR8 hom Protein involved in recovery from cell cycle arrest in response to pheromone, in a Far1p-independent pathway; interacts with Far3p, Far7p, Far9p, Far10p, and Far11p
0.101 4.05E-9 YMR022W UBC7 hom Ubiquitin conjugating enzyme, involved in the ER-associated protein degradation pathway; requires Cue1p for recruitment to the ER membrane; proposed to be involved in chromatin assembly