YHR087W / RTC3

Protein of unknown function involved in RNA metabolism; has structural similarity to SBDS, the human protein mutated in Shwachman-Diamond Syndrome (the yeast SBDS ortholog = SDO1); null mutation suppresses cdc13-1 temperature sensitivity; protein abundance increases in response to DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR087W / RTC3

Click on Significant Values for Screen Details ID:SGTC_58|Compound:0957-0393|FD-Score:5.08|P-value:2.82E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:-5.58|P-value:2.87E-7 ID:SGTC_165|Compound:k038-0023|FD-Score:5.12|P-value:2.38E-6 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:5.61|P-value:2.60E-7 ID:SGTC_577|Compound:r071-0003|FD-Score:3.70|P-value:4.70E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.58|P-value:6.80E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:3.91|P-value:2.39E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.55|P-value:7.67E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:6.10|P-value:2.42E-8 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.66|P-value:5.29E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.67|P-value:1.47E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.67|P-value:5.23E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:4.17|P-value:9.65E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:-4.38|P-value:4.34E-5 ID:SGTC_1474|Compound:0330-0125|FD-Score:3.52|P-value:8.18E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.89|P-value:2.57E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.78|P-value:3.68E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-4.02|P-value:1.56E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.64|P-value:5.69E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.60|P-value:6.57E-4 ID:SGTC_1776|Compound:st049249|FD-Score:3.57|P-value:7.03E-4 ID:SGTC_1835|Compound:st008666|FD-Score:3.55|P-value:7.50E-4 ID:SGTC_1899|Compound:st063254|FD-Score:4.04|P-value:1.51E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.94|P-value:2.14E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.54|P-value:7.46E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.65|P-value:5.56E-4 ID:SGTC_2318|Compound:6029776|FD-Score:6.18|P-value:1.61E-8 ID:SGTC_2323|Compound:6148992|FD-Score:3.97|P-value:1.94E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.49|P-value:8.98E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.55|P-value:7.63E-4 ID:SGTC_2368|Compound:9071271|FD-Score:5.17|P-value:1.88E-6 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-3.52|P-value:8.03E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:5.02|P-value:3.65E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:10.30|P-value:1.56E-20 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.87|P-value:2.62E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.84|P-value:2.82E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.09|P-value:1.27E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.59|P-value:6.69E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-5.59|P-value:2.73E-7 ID:SGTC_2907|Compound:7721985|FD-Score:-3.98|P-value:1.76E-4 ID:SGTC_2908|Compound:7930301|FD-Score:4.58|P-value:2.10E-5 ID:SGTC_3050|Compound:9093513|FD-Score:3.54|P-value:7.90E-4 ID:SGTC_3065|Compound:9113888|FD-Score:3.76|P-value:3.86E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-3.70|P-value:4.56E-4 ID:SGTC_3149|Compound:9097855|FD-Score:4.77|P-value:1.01E-5 ID:SGTC_58|Compound:0957-0393|FD-Score:5.08|P-value:2.82E-6 ID:SGTC_103|Compound:0844-0104|FD-Score:-5.58|P-value:2.87E-7 ID:SGTC_165|Compound:k038-0023|FD-Score:5.12|P-value:2.38E-6 ID:SGTC_388|Compound:3',4'-dichloroisocoumarin|FD-Score:5.61|P-value:2.60E-7 ID:SGTC_577|Compound:r071-0003|FD-Score:3.70|P-value:4.70E-4 ID:SGTC_673|Compound:1082-0474|FD-Score:3.58|P-value:6.80E-4 ID:SGTC_888|Compound:0916-0002|FD-Score:3.91|P-value:2.39E-4 ID:SGTC_1072|Compound:oxiconazole|FD-Score:3.55|P-value:7.67E-4 ID:SGTC_1083|Compound:didemethylchlorpromazine sulfoxide|FD-Score:6.10|P-value:2.42E-8 ID:SGTC_1112|Compound:tnp00069|FD-Score:3.66|P-value:5.29E-4 ID:SGTC_1196|Compound:1588-0294|FD-Score:4.67|P-value:1.47E-5 ID:SGTC_1282|Compound:0886-0010|FD-Score:3.67|P-value:5.23E-4 ID:SGTC_1306|Compound:1185-0270|FD-Score:4.17|P-value:9.65E-5 ID:SGTC_1465|Compound:k213-0056|FD-Score:-4.38|P-value:4.34E-5 ID:SGTC_1474|Compound:0330-0125|FD-Score:3.52|P-value:8.18E-4 ID:SGTC_1513|Compound:0337-0559|FD-Score:3.89|P-value:2.57E-4 ID:SGTC_1635|Compound:st007035|FD-Score:3.78|P-value:3.68E-4 ID:SGTC_1717|Compound:st033143|FD-Score:-4.02|P-value:1.56E-4 ID:SGTC_1719|Compound:st032227|FD-Score:3.64|P-value:5.69E-4 ID:SGTC_1773|Compound:st048542|FD-Score:3.60|P-value:6.57E-4 ID:SGTC_1776|Compound:st049249|FD-Score:3.57|P-value:7.03E-4 ID:SGTC_1835|Compound:st008666|FD-Score:3.55|P-value:7.50E-4 ID:SGTC_1899|Compound:st063254|FD-Score:4.04|P-value:1.51E-4 ID:SGTC_1952|Compound:st077036|FD-Score:3.94|P-value:2.14E-4 ID:SGTC_2010|Compound:4012422|FD-Score:-3.54|P-value:7.46E-4 ID:SGTC_2099|Compound:5377090|FD-Score:3.65|P-value:5.56E-4 ID:SGTC_2318|Compound:6029776|FD-Score:6.18|P-value:1.61E-8 ID:SGTC_2323|Compound:6148992|FD-Score:3.97|P-value:1.94E-4 ID:SGTC_2329|Compound:6138490|FD-Score:3.49|P-value:8.98E-4 ID:SGTC_2366|Compound:9070441|FD-Score:3.55|P-value:7.63E-4 ID:SGTC_2368|Compound:9071271|FD-Score:5.17|P-value:1.88E-6 ID:SGTC_2503|Compound:beta-caryophyllene alcohol|FD-Score:-3.52|P-value:8.03E-4 ID:SGTC_2538|Compound:3',7'-epoxycaryophyllan-6-one|FD-Score:5.02|P-value:3.65E-6 ID:SGTC_2582|Compound:agaric acid|FD-Score:10.30|P-value:1.56E-20 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:-3.87|P-value:2.62E-4 ID:SGTC_2621|Compound:lithochol-11-enic acid|FD-Score:-3.84|P-value:2.82E-4 ID:SGTC_2651|Compound:1327-0036|FD-Score:4.09|P-value:1.27E-4 ID:SGTC_2679|Compound:rapamycin|FD-Score:3.59|P-value:6.69E-4 ID:SGTC_2688|Compound:kanamycin b|FD-Score:-5.59|P-value:2.73E-7 ID:SGTC_2907|Compound:7721985|FD-Score:-3.98|P-value:1.76E-4 ID:SGTC_2908|Compound:7930301|FD-Score:4.58|P-value:2.10E-5 ID:SGTC_3050|Compound:9093513|FD-Score:3.54|P-value:7.90E-4 ID:SGTC_3065|Compound:9113888|FD-Score:3.76|P-value:3.86E-4 ID:SGTC_3108|Compound:9121785|FD-Score:-3.70|P-value:4.56E-4 ID:SGTC_3149|Compound:9097855|FD-Score:4.77|P-value:1.01E-5

Top fitness defect scores for YHR087W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2582 agaric acid 10.30 100.0 µM 1.56E-20 redox potentiating 58 3.62E-25
2 SGTC_2318 6029776 6.18 164.0 µM 1.61E-8 5 3.18E-10
3 SGTC_1083 didemethylchlorpromazine sulfoxide 6.10 707.4 nM 2.42E-8 redox potentiating 26 5.27E-10
4 SGTC_388 3',4'-dichloroisocoumarin 5.61 16.7 µM 2.60E-7 4 1.00E-8
5 SGTC_2368 9071271 5.17 200.0 µM 1.88E-6 9 1.16E-7
6 SGTC_165 k038-0023 5.12 64.4 µM 2.38E-6 ubiquinone biosynthesis & proteasome 39 1.56E-7
7 SGTC_58 0957-0393 5.08 9.0 µM 2.82E-6 cell wall 278 1.92E-7
8 SGTC_2538 3',7'-epoxycaryophyllan-6-one 5.02 100.0 µM 3.65E-6 9 2.65E-7
9 SGTC_3149 9097855 4.77 49.5 µM 1.01E-5 Golgi 9 9.28E-7
10 SGTC_1196 1588-0294 4.67 184.0 µM 1.47E-5 7 1.49E-6
11 SGTC_2908 7930301 4.58 100.0 µM 2.10E-5 14 2.31E-6
12 SGTC_1306 1185-0270 4.17 9.0 µM 9.65E-5 Golgi 49 1.52E-5
13 SGTC_2651 1327-0036 4.09 5.0 µM 1.27E-4 149 2.14E-5
14 SGTC_1899 st063254 4.04 59.6 µM 1.51E-4 12 2.65E-5
15 SGTC_2323 6148992 3.97 148.3 µM 1.94E-4 32 3.59E-5
16 SGTC_1952 st077036 3.94 64.1 µM 2.14E-4 35 4.07E-5
17 SGTC_888 0916-0002 3.91 7.0 µM 2.39E-4 NEO1 43 4.65E-5
18 SGTC_1513 0337-0559 3.89 19.0 µM 2.57E-4 20 5.10E-5
19 SGTC_1635 st007035 3.78 42.4 µM 3.68E-4 224 7.93E-5
20 SGTC_3065 9113888 3.76 49.5 µM 3.86E-4 28 8.42E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.245 3.14E-47 YML103C NUP188 hom Subunit of the inner ring of the nuclear pore complex (NPC); contributes to NPC organization and nucleocytoplasmic transport; homologous to human NUP188
0.212 2.39E-35 YMR109W MYO5 hom One of two type I myosins; contains proline-rich tail homology 2 (TH2) and SH3 domains; MYO5 deletion has little effect on growth, but myo3 myo5 double deletion causes severe defects in growth and actin cytoskeleton organization
0.206 2.49E-33 YNL106C INP52 hom Polyphosphatidylinositol phosphatase, dephosphorylates a number of phosphatidylinositols (PIs) to PI; involved in endocytosis; hyperosmotic stress causes translocation to actin patches; synaptojanin-like protein with a Sac1 domain
0.202 3.38E-32 YHR036W BRL1 het Essential nuclear envelope integral membrane protein identified as a suppressor of a conditional mutation in the major karyopherin, CRM1; homologous to and interacts with Brr6p, a nuclear envelope protein involved in nuclear export
0.192 2.69E-29 YOR049C RSB1 hom Suppressor of sphingoid long chain base (LCB) sensitivity of an LCB-lyase mutation; putative integral membrane transporter or flippase that may transport LCBs from the cytoplasmic side toward the extracytoplasmic side of the membrane
0.181 4.32E-26 YKL028W TFA1 het TFIIE large subunit, involved in recruitment of RNA polymerase II to the promoter, activation of TFIIH, and promoter opening
0.179 1.95E-25 YEL007W MIT1 hom Transcriptional regulator of pseudohyphal growth; protein with sequence similarity to S. pombe gti1+ (gluconate transport inducer 1) and C. albicans Wor1
0.178 3.66E-25 YNL215W IES2 hom Protein that associates with the INO80 chromatin remodeling complex; associates with the INO80 complex under low-salt conditions; essential for growth under anaerobic conditions; protein abundance increases in response to DNA replication stress
0.176 1.16E-24 YNL027W CRZ1 hom Transcription factor, activates transcription of stress response genes; nuclear localization is positively regulated by calcineurin-mediated dephosphorylation; rapidly localizes to the nucleus under blue light stress
0.173 4.94E-24 YJL054W TIM54 het Component of the mitochondrial TIM22 complex involved in insertion of polytopic proteins into the inner membrane
0.172 1.26E-23 YPL232W SSO1 hom Plasma membrane t-SNARE involved in fusion of secretory vesicles at the plasma membrane and in vesicle fusion during sporulation; forms a complex with Sec9p that binds v-SNARE Snc2p; syntaxin homolog; functionally redundant with Sso2p
0.170 3.58E-23 YIL160C POT1 hom 3-ketoacyl-CoA thiolase with broad chain length specificity, cleaves 3-ketoacyl-CoA into acyl-CoA and acetyl-CoA during beta-oxidation of fatty acids
0.168 1.13E-22 YGL178W MPT5 hom mRNA-binding protein of the PUF family; binds to the 3' UTR of specific mRNAs, including those involved in mating type switching, cell wall integrity, chronological lifespan, chromatin modification, and spindle pole body architecture; recruits the CCR4-NOT deadenylase complex to mRNAs along with Dhh1p and Dcp1p to promote deadenylation, decapping, and decay; also interacts with the Caf20p translational initiation repressor, affecting its mRNA target specificity
0.168 1.53E-22 YBR082C UBC4 hom Ubiquitin-conjugating enzyme (E2); mediates degradation of abnormal or excess proteins, including calmodulin and histone H3; interacts with many SCF ubiquitin protein ligases; component of the cellular stress response; UBC4 has a paralog, UBC5, that arose from the whole genome duplication
0.165 5.64E-22 YPL251W_d YPL251W_d het Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data; partially overlaps 5' end of the verified, essential gene YAH1/YPL252C