YHR088W / RPF1

Nucleolar protein involved in the assembly and export of the large ribosomal subunit; constituent of 66S pre-ribosomal particles; contains a sigma(70)-like motif, which is thought to bind RNA

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR088W / RPF1

Click on Significant Values for Screen Details ID:SGTC_119|Compound:0121-0043|FD-Score:4.02|P-value:6.96E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:5.60|P-value:5.63E-8 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.35|P-value:7.92E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.42|P-value:5.87E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:3.36|P-value:7.36E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.38|P-value:6.70E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.90|P-value:1.17E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.45|P-value:5.69E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.60|P-value:3.21E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.45|P-value:5.62E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.36|P-value:7.19E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:7.39|P-value:1.33E-12 ID:SGTC_1649|Compound:st011737|FD-Score:3.77|P-value:1.74E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.61|P-value:3.10E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.84|P-value:1.60E-8 ID:SGTC_1716|Compound:st033144|FD-Score:5.75|P-value:2.59E-8 ID:SGTC_1799|Compound:st045822|FD-Score:3.33|P-value:7.90E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:-3.53|P-value:4.26E-4 ID:SGTC_1868|Compound:st057644|FD-Score:5.30|P-value:2.57E-7 ID:SGTC_1966|Compound:st070145|FD-Score:5.49|P-value:1.01E-7 ID:SGTC_1972|Compound:st070201|FD-Score:-3.37|P-value:7.31E-4 ID:SGTC_2144|Compound:5628481|FD-Score:4.24|P-value:2.99E-5 ID:SGTC_2168|Compound:5727979|FD-Score:-3.50|P-value:4.72E-4 ID:SGTC_2215|Compound:7188065|FD-Score:4.39|P-value:1.58E-5 ID:SGTC_2258|Compound:7960471|FD-Score:-3.51|P-value:4.62E-4 ID:SGTC_2334|Compound:9017922|FD-Score:-3.69|P-value:2.43E-4 ID:SGTC_2335|Compound:9018011|FD-Score:8.04|P-value:1.31E-14 ID:SGTC_2338|Compound:7999974|FD-Score:-8.62|P-value:1.83E-16 ID:SGTC_2339|Compound:9000433|FD-Score:-3.90|P-value:1.15E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.05|P-value:6.22E-5 ID:SGTC_2443|Compound:5493264|FD-Score:4.04|P-value:6.42E-5 ID:SGTC_2703|Compound:st077852|FD-Score:4.41|P-value:1.47E-5 ID:SGTC_2715|Compound:st079500|FD-Score:-3.54|P-value:4.21E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.47|P-value:5.33E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.19|P-value:3.53E-5 ID:SGTC_2821|Compound:7996764|FD-Score:-5.28|P-value:3.03E-7 ID:SGTC_2825|Compound:7998094|FD-Score:3.62|P-value:3.02E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.68|P-value:2.44E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.57|P-value:3.73E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.84|P-value:1.39E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.74|P-value:1.97E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.53|P-value:4.16E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.46|P-value:5.14E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.53|P-value:4.08E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.50|P-value:4.53E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.51|P-value:4.62E-4 ID:SGTC_119|Compound:0121-0043|FD-Score:4.02|P-value:6.96E-5 ID:SGTC_124|Compound:0136-0217|FD-Score:5.60|P-value:5.63E-8 ID:SGTC_181|Compound:1447-1644|FD-Score:-3.35|P-value:7.92E-4 ID:SGTC_264|Compound:promethazine|FD-Score:3.42|P-value:5.87E-4 ID:SGTC_788|Compound:1182-0090|FD-Score:3.36|P-value:7.36E-4 ID:SGTC_1032|Compound:k061-1018|FD-Score:3.38|P-value:6.70E-4 ID:SGTC_1100|Compound:amiodarone|FD-Score:-3.90|P-value:1.17E-4 ID:SGTC_1136|Compound:pergolide|FD-Score:-3.45|P-value:5.69E-4 ID:SGTC_1213|Compound:1315-0166|FD-Score:3.60|P-value:3.21E-4 ID:SGTC_1386|Compound:3228-0095|FD-Score:-3.45|P-value:5.62E-4 ID:SGTC_1449|Compound:4112-4002|FD-Score:3.36|P-value:7.19E-4 ID:SGTC_1457|Compound:k015-0032|FD-Score:7.39|P-value:1.33E-12 ID:SGTC_1649|Compound:st011737|FD-Score:3.77|P-value:1.74E-4 ID:SGTC_1681|Compound:tripelennamine|FD-Score:3.61|P-value:3.10E-4 ID:SGTC_1713|Compound:st033146|FD-Score:5.84|P-value:1.60E-8 ID:SGTC_1716|Compound:st033144|FD-Score:5.75|P-value:2.59E-8 ID:SGTC_1799|Compound:st045822|FD-Score:3.33|P-value:7.90E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:-3.53|P-value:4.26E-4 ID:SGTC_1868|Compound:st057644|FD-Score:5.30|P-value:2.57E-7 ID:SGTC_1966|Compound:st070145|FD-Score:5.49|P-value:1.01E-7 ID:SGTC_1972|Compound:st070201|FD-Score:-3.37|P-value:7.31E-4 ID:SGTC_2144|Compound:5628481|FD-Score:4.24|P-value:2.99E-5 ID:SGTC_2168|Compound:5727979|FD-Score:-3.50|P-value:4.72E-4 ID:SGTC_2215|Compound:7188065|FD-Score:4.39|P-value:1.58E-5 ID:SGTC_2258|Compound:7960471|FD-Score:-3.51|P-value:4.62E-4 ID:SGTC_2334|Compound:9017922|FD-Score:-3.69|P-value:2.43E-4 ID:SGTC_2335|Compound:9018011|FD-Score:8.04|P-value:1.31E-14 ID:SGTC_2338|Compound:7999974|FD-Score:-8.62|P-value:1.83E-16 ID:SGTC_2339|Compound:9000433|FD-Score:-3.90|P-value:1.15E-4 ID:SGTC_2407|Compound:paclitaxel|FD-Score:4.05|P-value:6.22E-5 ID:SGTC_2443|Compound:5493264|FD-Score:4.04|P-value:6.42E-5 ID:SGTC_2703|Compound:st077852|FD-Score:4.41|P-value:1.47E-5 ID:SGTC_2715|Compound:st079500|FD-Score:-3.54|P-value:4.21E-4 ID:SGTC_2805|Compound:7950783|FD-Score:-3.47|P-value:5.33E-4 ID:SGTC_2806|Compound:7952793|FD-Score:4.19|P-value:3.53E-5 ID:SGTC_2821|Compound:7996764|FD-Score:-5.28|P-value:3.03E-7 ID:SGTC_2825|Compound:7998094|FD-Score:3.62|P-value:3.02E-4 ID:SGTC_2937|Compound:9044753|FD-Score:3.68|P-value:2.44E-4 ID:SGTC_2991|Compound:9074669|FD-Score:-3.57|P-value:3.73E-4 ID:SGTC_3016|Compound:9080866|FD-Score:3.84|P-value:1.39E-4 ID:SGTC_3018|Compound:9082602|FD-Score:3.74|P-value:1.97E-4 ID:SGTC_3093|Compound:9117344|FD-Score:3.53|P-value:4.16E-4 ID:SGTC_3136|Compound:9095672|FD-Score:3.46|P-value:5.14E-4 ID:SGTC_3187|Compound:9109394|FD-Score:3.53|P-value:4.08E-4 ID:SGTC_3188|Compound:9110789|FD-Score:3.50|P-value:4.53E-4 ID:SGTC_3300|Compound:9124615|FD-Score:-3.51|P-value:4.62E-4

Top fitness defect scores for YHR088W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2335 9018011 8.04 154.2 µM 1.31E-14 1 4.34E-16 2.00
2 SGTC_1457 k015-0032 7.39 57.3 µM 1.33E-12 1 7.42E-14 1.56
3 SGTC_1713 st033146 5.84 12.8 µM 1.60E-8 1 2.62E-9 0.37
4 SGTC_1716 st033144 5.75 30.6 µM 2.59E-8 2 4.46E-9 0.70
5 SGTC_124 0136-0217 5.60 7.1 µM 5.63E-8 mitochondrial stress 11 1.06E-8 0.38
6 SGTC_1966 st070145 5.49 16.1 µM 1.01E-7 2 2.04E-8 0.26
7 SGTC_1868 st057644 5.30 42.7 µM 2.57E-7 3 5.73E-8 0.91
8 SGTC_2703 st077852 4.41 53.4 µM 1.47E-5 4 5.16E-6 0.17
9 SGTC_2215 7188065 4.39 200.0 µM 1.58E-5 2 5.60E-6 0.71
10 SGTC_2144 5628481 4.24 200.0 µM 2.99E-5 ERG2 1 1.14E-5 0.63
11 SGTC_2806 7952793 4.19 58.4 µM 3.53E-5 4 1.37E-5 0.22
12 SGTC_2407 paclitaxel 4.05 400.0 µM 6.22E-5 5 2.56E-5 0.13
13 SGTC_2443 5493264 4.04 126.7 µM 6.42E-5 6 2.65E-5 0.27
14 SGTC_119 0121-0043 4.02 24.8 µM 6.96E-5 mitochondrial stress 21 2.90E-5 0.06
15 SGTC_3016 9080866 3.84 49.5 µM 1.39E-4 4 6.24E-5 0.26
16 SGTC_1649 st011737 3.77 51.3 µM 1.74E-4 2 8.03E-5 0.13
17 SGTC_3018 9082602 3.74 49.5 µM 1.97E-4 8 9.19E-5 0.02
18 SGTC_2937 9044753 3.68 11.3 µM 2.44E-4 60S ribosome export 11 1.17E-4 0.19
19 SGTC_2825 7998094 3.62 58.4 µM 3.02E-4 fatty acid desaturase (OLE1) 15 1.48E-4 0.16
20 SGTC_1681 tripelennamine 3.61 100.0 µM 3.10E-4 14 1.52E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.138 1.02E-15 YOR109W INP53 hom Polyphosphatidylinositol phosphatase, dephosphorylates multiple phosphatidylinositols; involved in trans Golgi network-to-early endosome pathway; hyperosmotic stress causes translocation to actin patches; contains Sac1 and 5-ptase domains
0.135 3.86E-15 YPL182C_d YPL182C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene CTI6/YPL181W
0.097 2.07E-8 YPL038W-A_p YPL038W-A_p hom Putative protein of unknown function; identified by fungal homology and RT-PCR
0.090 1.62E-7 YOR232W MGE1 het Mitochondrial matrix cochaperone, acts as a nucleotide release factor for Ssc1p in protein translocation and folding; also acts as cochaperone for Ssq1p in folding of Fe-S cluster proteins; homolog of E. coli GrpE
0.086 6.30E-7 YMR271C URA10 hom Minor orotate phosphoribosyltransferase (OPRTase) isozyme; catalyzes the fifth enzymatic step in the de novo biosynthesis of pyrimidines, converting orotate into orotidine-5'-phosphate; URA10 has a paralog, URA5, that arose from the whole genome duplication
0.084 1.02E-6 YLL040C VPS13 hom Protein of unknown function; heterooligomeric or homooligomeric complex; peripherally associated with membranes; involved in sporulation, vacuolar protein sorting, prospore membrane formation and protein-Golgi retention; homologous to human CHAC and COH1 which are involved in chorea acanthocytosis and Cohen syndrome, respectively
0.084 1.14E-6 YPR051W MAK3 hom Catalytic subunit of N-terminal acetyltransferase of the NatC type; required for replication of dsRNA virus
0.082 2.22E-6 YLR390W ECM19 hom Putative protein of unknown function; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.079 4.08E-6 YDL237W AIM6 hom Putative protein of unknown function, required for respiratory growth; YDL237W is not an essential gene
0.079 4.81E-6 YBR225W_p YBR225W_p hom Putative protein of unknown function; non-essential gene identified in a screen for mutants affected in mannosylphophorylation of cell wall components
0.078 5.60E-6 YLR448W RPL6B hom Ribosomal 60S subunit protein L6B; binds 5.8S rRNA; homologous to mammalian ribosomal protein L6, no bacterial homolog; RPL6B has a paralog, RPL6A, that arose from the whole genome duplication
0.078 6.37E-6 YPL091W GLR1 hom Cytosolic and mitochondrial glutathione oxidoreductase; converts oxidized glutathione to reduced glutathione; mitochondrial but not cytosolic form has a role in resistance to hyperoxia; protein abundance increases in response to DNA replication stress
0.076 1.04E-5 YPR050C_d YPR050C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene MAK3/YPR051W
0.075 1.39E-5 YPL118W MRP51 hom Mitochondrial ribosomal protein of the small subunit; MRP51 exhibits genetic interactions with mutations in the COX2 and COX3 mRNA 5'-untranslated leader sequences
0.073 2.48E-5 YLR191W PEX13 hom Integral peroxisomal membrane protein required for translocation of peroxisomal matrix proteins, interacts with the PTS1 signal recognition factor Pex5p and the PTS2 signal recognition factor Pex7p, forms a complex with Pex14p and Pex17p