YHR101C / BIG1

Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR101C / BIG1

Click on Significant Values for Screen Details ID:SGTC_16|Compound:erodoxin|FD-Score:4.98|P-value:1.68E-8 ID:SGTC_44|Compound:0929-0055|FD-Score:3.01|P-value:4.25E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:3.43|P-value:7.10E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.69|P-value:2.12E-5 ID:SGTC_246|Compound:cycloheximide|FD-Score:2.87|P-value:7.30E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:-2.96|P-value:5.10E-4 ID:SGTC_276|Compound:colchicine|FD-Score:2.94|P-value:5.65E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.38|P-value:8.99E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.20|P-value:1.96E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.22|P-value:1.77E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:3.34|P-value:1.04E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.99|P-value:4.95E-6 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.59|P-value:3.46E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-6.36|P-value:8.71E-13 ID:SGTC_1085|Compound:doxorubicin|FD-Score:2.93|P-value:5.70E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:2.92|P-value:5.97E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:2.80|P-value:9.67E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.80|P-value:1.23E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:2.84|P-value:8.20E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.60|P-value:3.23E-5 ID:SGTC_1388|Compound:3346-4487|FD-Score:2.81|P-value:9.00E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.02|P-value:4.02E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:3.18|P-value:2.08E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:5.89|P-value:3.32E-11 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.10|P-value:2.87E-4 ID:SGTC_1702|Compound:st027038|FD-Score:3.13|P-value:2.55E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.26|P-value:1.49E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.82|P-value:1.16E-5 ID:SGTC_1723|Compound:st034307|FD-Score:3.38|P-value:8.95E-5 ID:SGTC_1809|Compound:st050081|FD-Score:4.49|P-value:3.23E-7 ID:SGTC_1813|Compound:st051246|FD-Score:2.89|P-value:6.76E-4 ID:SGTC_1815|Compound:st051655|FD-Score:2.79|P-value:9.94E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.11|P-value:2.78E-4 ID:SGTC_1968|Compound:st069590|FD-Score:5.55|P-value:3.77E-10 ID:SGTC_1985|Compound:st071382|FD-Score:2.87|P-value:7.22E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.37|P-value:9.33E-5 ID:SGTC_2146|Compound:5545642|FD-Score:-3.93|P-value:6.41E-6 ID:SGTC_2150|Compound:5556613|FD-Score:-2.94|P-value:5.63E-4 ID:SGTC_2191|Compound:5911152|FD-Score:2.79|P-value:9.81E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-3.77|P-value:1.43E-5 ID:SGTC_2343|Compound:7990716|FD-Score:-2.95|P-value:5.43E-4 ID:SGTC_2398|Compound:5228213|FD-Score:4.99|P-value:1.59E-8 ID:SGTC_2399|Compound:5884133|FD-Score:6.48|P-value:3.22E-13 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.26|P-value:1.47E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:2.82|P-value:8.70E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:-3.13|P-value:2.55E-4 ID:SGTC_2806|Compound:7952793|FD-Score:-3.07|P-value:3.36E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-4.39|P-value:5.62E-7 ID:SGTC_3233|Compound:9132340|FD-Score:4.53|P-value:2.53E-7 ID:SGTC_3254|Compound:9136651|FD-Score:3.07|P-value:3.35E-4 ID:SGTC_3333|Compound:9141820|FD-Score:2.85|P-value:7.96E-4 ID:SGTC_16|Compound:erodoxin|FD-Score:4.98|P-value:1.68E-8 ID:SGTC_44|Compound:0929-0055|FD-Score:3.01|P-value:4.25E-4 ID:SGTC_95|Compound:0471-0001|FD-Score:3.43|P-value:7.10E-5 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.69|P-value:2.12E-5 ID:SGTC_246|Compound:cycloheximide|FD-Score:2.87|P-value:7.30E-4 ID:SGTC_265|Compound:mycophenolic acid|FD-Score:-2.96|P-value:5.10E-4 ID:SGTC_276|Compound:colchicine|FD-Score:2.94|P-value:5.65E-4 ID:SGTC_513|Compound:4245-1575|FD-Score:3.38|P-value:8.99E-5 ID:SGTC_577|Compound:r071-0003|FD-Score:3.20|P-value:1.96E-4 ID:SGTC_785|Compound:1270-0111|FD-Score:-3.22|P-value:1.77E-4 ID:SGTC_809|Compound:3998-0021|FD-Score:3.34|P-value:1.04E-4 ID:SGTC_817|Compound:1391-0715|FD-Score:3.99|P-value:4.95E-6 ID:SGTC_1000|Compound:4048-0884|FD-Score:3.59|P-value:3.46E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:-6.36|P-value:8.71E-13 ID:SGTC_1085|Compound:doxorubicin|FD-Score:2.93|P-value:5.70E-4 ID:SGTC_1243|Compound:0416-0003|FD-Score:2.92|P-value:5.97E-4 ID:SGTC_1251|Compound:0490-5699|FD-Score:2.80|P-value:9.67E-4 ID:SGTC_1307|Compound:1187-1599|FD-Score:3.80|P-value:1.23E-5 ID:SGTC_1346|Compound:1488-2224|FD-Score:2.84|P-value:8.20E-4 ID:SGTC_1360|Compound:1598-0022|FD-Score:3.60|P-value:3.23E-5 ID:SGTC_1388|Compound:3346-4487|FD-Score:2.81|P-value:9.00E-4 ID:SGTC_1462|Compound:k081-0014|FD-Score:-3.02|P-value:4.02E-4 ID:SGTC_1491|Compound:4138-0063|FD-Score:3.18|P-value:2.08E-4 ID:SGTC_1527|Compound:coumarin|FD-Score:5.89|P-value:3.32E-11 ID:SGTC_1580|Compound:hydrocortisone 21-hemisuccinate|FD-Score:3.10|P-value:2.87E-4 ID:SGTC_1702|Compound:st027038|FD-Score:3.13|P-value:2.55E-4 ID:SGTC_1708|Compound:st033234|FD-Score:3.26|P-value:1.49E-4 ID:SGTC_1713|Compound:st033146|FD-Score:-3.82|P-value:1.16E-5 ID:SGTC_1723|Compound:st034307|FD-Score:3.38|P-value:8.95E-5 ID:SGTC_1809|Compound:st050081|FD-Score:4.49|P-value:3.23E-7 ID:SGTC_1813|Compound:st051246|FD-Score:2.89|P-value:6.76E-4 ID:SGTC_1815|Compound:st051655|FD-Score:2.79|P-value:9.94E-4 ID:SGTC_1817|Compound:st052116|FD-Score:3.11|P-value:2.78E-4 ID:SGTC_1968|Compound:st069590|FD-Score:5.55|P-value:3.77E-10 ID:SGTC_1985|Compound:st071382|FD-Score:2.87|P-value:7.22E-4 ID:SGTC_2063|Compound:5246145|FD-Score:3.37|P-value:9.33E-5 ID:SGTC_2146|Compound:5545642|FD-Score:-3.93|P-value:6.41E-6 ID:SGTC_2150|Compound:5556613|FD-Score:-2.94|P-value:5.63E-4 ID:SGTC_2191|Compound:5911152|FD-Score:2.79|P-value:9.81E-4 ID:SGTC_2333|Compound:9017922|FD-Score:-3.77|P-value:1.43E-5 ID:SGTC_2343|Compound:7990716|FD-Score:-2.95|P-value:5.43E-4 ID:SGTC_2398|Compound:5228213|FD-Score:4.99|P-value:1.59E-8 ID:SGTC_2399|Compound:5884133|FD-Score:6.48|P-value:3.22E-13 ID:SGTC_2501|Compound:sodium tetradecyl sulfate|FD-Score:3.26|P-value:1.47E-4 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:2.82|P-value:8.70E-4 ID:SGTC_2727|Compound:riluzole|FD-Score:-3.13|P-value:2.55E-4 ID:SGTC_2806|Compound:7952793|FD-Score:-3.07|P-value:3.36E-4 ID:SGTC_2972|Compound:9093619|FD-Score:-4.39|P-value:5.62E-7 ID:SGTC_3233|Compound:9132340|FD-Score:4.53|P-value:2.53E-7 ID:SGTC_3254|Compound:9136651|FD-Score:3.07|P-value:3.35E-4 ID:SGTC_3333|Compound:9141820|FD-Score:2.85|P-value:7.96E-4

Top fitness defect scores for YHR101C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2399 5884133 6.48 200.0 µM 3.22E-13 cell wall signaling 4 4.47E-11 0.20
2 SGTC_1527 coumarin 5.89 137.0 µM 3.32E-11 3 1.97E-9 1.06
3 SGTC_1968 st069590 5.55 43.6 µM 3.77E-10 9 1.43E-8 0.54
4 SGTC_2398 5228213 4.99 48.8 µM 1.59E-8 iron homeostasis 9 3.05E-7 0.18
5 SGTC_16 erodoxin 4.98 22.8 µM 1.68E-8 unfolded protein response 19 3.20E-7 0.11
6 SGTC_3233 9132340 4.53 49.5 µM 2.53E-7 5 2.94E-6 0.43
7 SGTC_1809 st050081 4.49 57.6 µM 3.23E-7 3 3.60E-6 0.08
8 SGTC_817 1391-0715 3.99 46.0 µM 4.95E-6 7 3.37E-5 0.05
9 SGTC_1307 1187-1599 3.80 12.2 µM 1.23E-5 PDR1 4 7.11E-5 0.16
10 SGTC_243 5hr heat shock (37°C) + splitomicin 3.69 1.0 µM 2.12E-5 heat shock/prefoldin 5 1.11E-4 0.10
11 SGTC_1360 1598-0022 3.60 39.8 µM 3.23E-5 ERG2 13 1.57E-4 0.06
12 SGTC_1000 4048-0884 3.59 8.7 µM 3.46E-5 copper-dependent oxidative stress 9 1.67E-4 0.02
13 SGTC_95 0471-0001 3.43 561.1 µM 7.10E-5 8 3.01E-4 0.13
14 SGTC_1723 st034307 3.38 2.5 µM 8.95E-5 mitochondrial processes 23 3.64E-4 0.02
15 SGTC_513 4245-1575 3.38 41.7 µM 8.99E-5 unfolded protein response 42 3.65E-4 0.02
16 SGTC_2063 5246145 3.37 200.0 µM 9.33E-5 11 3.77E-4 0.21
17 SGTC_809 3998-0021 3.34 90.0 µM 1.04E-4 RNA processing & uracil transport 15 4.14E-4 0.04
18 SGTC_2501 sodium tetradecyl sulfate 3.26 13.4 µM 1.47E-4 9 5.49E-4 0.01
19 SGTC_1708 st033234 3.26 31.8 µM 1.49E-4 9 5.54E-4 0.12
20 SGTC_577 r071-0003 3.20 38.6 µM 1.96E-4 fatty acid desaturase (OLE1) 12 6.96E-4 0.05

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.172 9.15E-24 YBR260C RGD1 hom GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.158 3.26E-20 YHR086W NAM8 hom RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
0.147 1.42E-17 YJR117W STE24 hom Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.146 1.79E-17 YML130C ERO1 het Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.145 2.68E-17 YPL244C HUT1 hom Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.138 7.93E-16 YDL045C FAD1 het Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.129 5.94E-14 YAL053W FLC2 hom Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.127 1.88E-13 YAL056C-A_d YAL056C-A_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.122 1.48E-12 YMR161W HLJ1 hom Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.112 8.21E-11 YGR185C TYS1 het Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.111 1.28E-10 YNL322C KRE1 hom Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.109 2.16E-10 YAL058W CNE1 hom Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.105 1.03E-9 YDR340W_d YDR340W_d hom Putative protein of unknown function
0.102 3.23E-9 YBR254C TRS20 het One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
0.102 3.48E-9 YBR079C RPG1 het eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation