HIPHOP chemogenomics database

YHR101C / BIG1

Integral membrane protein of the endoplasmic reticulum, required for normal content of cell wall beta-1,6-glucan

Zygosity: Heterozygous strain

fixedexpanded
Profile for YHR101C / BIG1

Click on Significant Values for Screen Details

Top fitness defect scores for YHR101C deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_2399	5884133	6.48	200.0 µM	3.22E-13	cell wall signaling	4	4.47E-11	0.20
2	SGTC_1527	coumarin	5.89	137.0 µM	3.32E-11		3	1.97E-9	1.06
3	SGTC_1968	st069590	5.55	43.6 µM	3.77E-10		9	1.43E-8	0.54
4	SGTC_2398	5228213	4.99	48.8 µM	1.59E-8	iron homeostasis	9	3.05E-7	0.18
5	SGTC_16	erodoxin	4.98	22.8 µM	1.68E-8	unfolded protein response	19	3.20E-7	0.11
6	SGTC_3233	9132340	4.53	49.5 µM	2.53E-7		5	2.94E-6	0.43
7	SGTC_1809	st050081	4.49	57.6 µM	3.23E-7		3	3.60E-6	0.08
8	SGTC_817	1391-0715	3.99	46.0 µM	4.95E-6		7	3.37E-5	0.05
9	SGTC_1307	1187-1599	3.80	12.2 µM	1.23E-5	PDR1	4	7.11E-5	0.16
10	SGTC_243	5hr heat shock (37°C) + splitomicin	3.69	1.0 µM	2.12E-5	heat shock/prefoldin	5	1.11E-4	0.10
11	SGTC_1360	1598-0022	3.60	39.8 µM	3.23E-5	ERG2	13	1.57E-4	0.06
12	SGTC_1000	4048-0884	3.59	8.7 µM	3.46E-5	copper-dependent oxidative stress	9	1.67E-4	0.02
13	SGTC_95	0471-0001	3.43	561.1 µM	7.10E-5		8	3.01E-4	0.13
14	SGTC_1723	st034307	3.38	2.5 µM	8.95E-5	mitochondrial processes	23	3.64E-4	0.02
15	SGTC_513	4245-1575	3.38	41.7 µM	8.99E-5	unfolded protein response	42	3.65E-4	0.02
16	SGTC_2063	5246145	3.37	200.0 µM	9.33E-5		11	3.77E-4	0.21
17	SGTC_809	3998-0021	3.34	90.0 µM	1.04E-4	RNA processing & uracil transport	15	4.14E-4	0.04
18	SGTC_2501	sodium tetradecyl sulfate	3.26	13.4 µM	1.47E-4		9	5.49E-4	0.01
19	SGTC_1708	st033234	3.26	31.8 µM	1.49E-4		9	5.54E-4	0.12
20	SGTC_577	r071-0003	3.20	38.6 µM	1.96E-4	fatty acid desaturase (OLE1)	12	6.96E-4	0.05

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.172	9.15E-24	YBR260C	RGD1	hom	GTPase-activating protein (RhoGAP) for Rho3p and Rho4p, possibly involved in control of actin cytoskeleton organization
0.158	3.26E-20	YHR086W	NAM8	hom	RNA binding protein, component of the U1 snRNP protein; mutants are defective in meiotic recombination and in formation of viable spores, involved in the formation of DSBs through meiosis-specific splicing of REC107 pre-mRNA; Nam8p regulon embraces the meiotic pre-mRNAs of REC107, HFM1, AMA1 SPO22 and PCH2; the putative RNA binding domains RRM2 and RRM3 are required for Nam8p meiotic function
0.147	1.42E-17	YJR117W	STE24	hom	Highly conserved zinc metalloprotease that functions in two steps of a-factor maturation, C-terminal CAAX proteolysis and the first step of N-terminal proteolytic processing; contains multiple transmembrane spans
0.146	1.79E-17	YML130C	ERO1	het	Thiol oxidase required for oxidative protein folding in the ER; essential for maintaining proper redox balance in ER; feedback regulation of Ero1p occurs via reduction and oxidation of Ero1p regulatory bonds; reduced Pdi1p activates Ero1p by direct reduction of Ero1p regulatory bonds; depletion of thiol substrates and accumulation of oxidized Pdi1p results in inactivation of Ero1p by both Pdi1p-mediated oxidation and autonomous oxidation of Ero1p regulatory bonds
0.145	2.68E-17	YPL244C	HUT1	hom	Protein with a role in UDP-galactose transport to the Golgi lumen, has similarity to human UDP-galactose transporter UGTrel1, exhibits a genetic interaction with S. cerevisiae ERO1
0.138	7.93E-16	YDL045C	FAD1	het	Flavin adenine dinucleotide (FAD) synthetase, performs the second step in synthesis of FAD from riboflavin
0.129	5.94E-14	YAL053W	FLC2	hom	Putative FAD transporter; required for uptake of FAD into endoplasmic reticulum; involved in cell wall maintenance; FLC2 has a paralog, YOR365C, that arose from the whole genome duplication
0.127	1.88E-13	YAL056C-A_d	YAL056C-A_d	hom	Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.122	1.48E-12	YMR161W	HLJ1	hom	Co-chaperone for Hsp40p, anchored in the ER membrane; with its homolog Ydj1p promotes ER-associated protein degradation (ERAD) of integral membrane substrates; similar to E. coli DnaJ
0.112	8.21E-11	YGR185C	TYS1	het	Cytoplasmic tyrosyl-tRNA synthetase; required for cytoplasmic protein synthesis; interacts with positions 34 and 35 of the tRNATyr anticodon; mutations in human ortholog YARS are associated with Charcot-Marie-Tooth (CMT) neuropathies; protein abundance increases in response to DNA replication stress
0.111	1.28E-10	YNL322C	KRE1	hom	Cell wall glycoprotein involved in beta-glucan assembly; serves as a K1 killer toxin membrane receptor
0.109	2.16E-10	YAL058W	CNE1	hom	Calnexin; integral membrane ER chaperone involved in folding and quality control of glycoproteins; chaperone activity is inhibited by Mpd1p, with which Cne1p interacts; 24% identical to mammalian calnexin; Ca+ binding not yet shown in yeast
0.105	1.03E-9	YDR340W_d	YDR340W_d	hom	Putative protein of unknown function
0.102	3.23E-9	YBR254C	TRS20	het	One of 10 subunits of the transport protein particle (TRAPP) complex of the cis-Golgi which mediates vesicle docking and fusion; mutations in the human homolog cause the spondyloepiphyseal dysplasia tarda (SEDL) disorder
0.102	3.48E-9	YBR079C	RPG1	het	eIF3a subunit of the core complex of translation initiation factor 3 (eIF3), essential for translation; part of a Prt1p-Rpg1p-Nip1p subcomplex that stimulates binding of mRNA and tRNA(i)Met to ribosomes; involved in translation reinitiation