YHR108W / GGA2

Protein that interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner to facilitate traffic through the late Golgi; binds phosphatidylinositol 4-phosphate, which plays a role in TGN localization; has homology to gamma-adaptin

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR108W / GGA2

Click on Significant Values for Screen Details ID:SGTC_49|Compound:0717-0965|FD-Score:4.68|P-value:7.30E-7 ID:SGTC_206|Compound:4546-0025|FD-Score:3.70|P-value:6.98E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:3.06|P-value:8.00E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:16.30|P-value:1.53E-63 ID:SGTC_301|Compound:4119-0100|FD-Score:6.12|P-value:1.48E-10 ID:SGTC_313|Compound:3474-0040|FD-Score:6.64|P-value:4.14E-12 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.35|P-value:2.82E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.71|P-value:6.77E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:5.18|P-value:4.69E-8 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.21|P-value:7.77E-11 ID:SGTC_449|Compound:xestospongin c|FD-Score:5.67|P-value:2.58E-9 ID:SGTC_535|Compound:2293-4129|FD-Score:4.83|P-value:3.29E-7 ID:SGTC_807|Compound:1315-0151|FD-Score:3.60|P-value:1.03E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:-4.24|P-value:6.23E-6 ID:SGTC_822|Compound:1487-0950|FD-Score:3.03|P-value:9.05E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.26|P-value:3.88E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.25|P-value:4.16E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:-3.73|P-value:6.12E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:3.53|P-value:1.40E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:3.89|P-value:3.07E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.01|P-value:9.54E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:5.01|P-value:1.24E-7 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.23|P-value:4.40E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.77|P-value:5.23E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.17|P-value:5.42E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.27|P-value:3.84E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:5.00|P-value:1.33E-7 ID:SGTC_1635|Compound:st007035|FD-Score:4.43|P-value:2.57E-6 ID:SGTC_1672|Compound:st016598|FD-Score:5.04|P-value:1.08E-7 ID:SGTC_1673|Compound:st016611|FD-Score:-3.61|P-value:9.81E-5 ID:SGTC_1754|Compound:st042065|FD-Score:16.60|P-value:1.12E-65 ID:SGTC_1757|Compound:st041620|FD-Score:4.23|P-value:6.66E-6 ID:SGTC_1758|Compound:st042942|FD-Score:3.01|P-value:9.76E-4 ID:SGTC_1760|Compound:st045453|FD-Score:31.20|P-value:5.65E-227 ID:SGTC_1762|Compound:st045503|FD-Score:5.61|P-value:3.72E-9 ID:SGTC_1766|Compound:st045414|FD-Score:6.94|P-value:4.30E-13 ID:SGTC_1781|Compound:st038725|FD-Score:4.88|P-value:2.54E-7 ID:SGTC_1801|Compound:st050089|FD-Score:4.59|P-value:1.15E-6 ID:SGTC_1803|Compound:st050068|FD-Score:4.86|P-value:2.81E-7 ID:SGTC_1923|Compound:st058458|FD-Score:3.92|P-value:2.75E-5 ID:SGTC_1986|Compound:st071812|FD-Score:3.22|P-value:4.59E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.51|P-value:1.52E-4 ID:SGTC_2036|Compound:5160441|FD-Score:4.05|P-value:1.49E-5 ID:SGTC_2060|Compound:5238662|FD-Score:3.50|P-value:1.56E-4 ID:SGTC_2221|Compound:6807166|FD-Score:3.02|P-value:9.37E-4 ID:SGTC_2257|Compound:7960413|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_2441|Compound:5730946|FD-Score:4.05|P-value:1.49E-5 ID:SGTC_2451|Compound:5856223|FD-Score:4.96|P-value:1.61E-7 ID:SGTC_2505|Compound:galangin|FD-Score:3.45|P-value:1.88E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.63|P-value:3.39E-9 ID:SGTC_2655|Compound:perillic acid|FD-Score:3.88|P-value:3.16E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.06|P-value:1.84E-13 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.78|P-value:5.05E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.69|P-value:7.11E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.14|P-value:6.08E-4 ID:SGTC_2807|Compound:7945238|FD-Score:7.17|P-value:7.80E-14 ID:SGTC_2808|Compound:7946468|FD-Score:7.56|P-value:3.43E-15 ID:SGTC_2896|Compound:9052656|FD-Score:4.57|P-value:1.28E-6 ID:SGTC_3037|Compound:9091026|FD-Score:3.22|P-value:4.65E-4 ID:SGTC_3152|Compound:9099253|FD-Score:5.30|P-value:2.43E-8 ID:SGTC_49|Compound:0717-0965|FD-Score:4.68|P-value:7.30E-7 ID:SGTC_206|Compound:4546-0025|FD-Score:3.70|P-value:6.98E-5 ID:SGTC_207|Compound:4185-0010|FD-Score:3.06|P-value:8.00E-4 ID:SGTC_221|Compound:trifluoperazine|FD-Score:16.30|P-value:1.53E-63 ID:SGTC_301|Compound:4119-0100|FD-Score:6.12|P-value:1.48E-10 ID:SGTC_313|Compound:3474-0040|FD-Score:6.64|P-value:4.14E-12 ID:SGTC_355|Compound:1311-2252|FD-Score:-3.35|P-value:2.82E-4 ID:SGTC_397|Compound:arbidol|FD-Score:3.71|P-value:6.77E-5 ID:SGTC_439|Compound:amiodarone|FD-Score:5.18|P-value:4.69E-8 ID:SGTC_445|Compound:e6 berbamine|FD-Score:6.21|P-value:7.77E-11 ID:SGTC_449|Compound:xestospongin c|FD-Score:5.67|P-value:2.58E-9 ID:SGTC_535|Compound:2293-4129|FD-Score:4.83|P-value:3.29E-7 ID:SGTC_807|Compound:1315-0151|FD-Score:3.60|P-value:1.03E-4 ID:SGTC_821|Compound:1114-0266|FD-Score:-4.24|P-value:6.23E-6 ID:SGTC_822|Compound:1487-0950|FD-Score:3.03|P-value:9.05E-4 ID:SGTC_829|Compound:1635-0120|FD-Score:3.26|P-value:3.88E-4 ID:SGTC_837|Compound:0091-0265|FD-Score:3.25|P-value:4.16E-4 ID:SGTC_848|Compound:0368-0089|FD-Score:-3.73|P-value:6.12E-5 ID:SGTC_900|Compound:1112-0045|FD-Score:3.53|P-value:1.40E-4 ID:SGTC_945|Compound:3381-0768|FD-Score:3.89|P-value:3.07E-5 ID:SGTC_1036|Compound:k216-0215|FD-Score:3.01|P-value:9.54E-4 ID:SGTC_1043|Compound:0916-0009|FD-Score:5.01|P-value:1.24E-7 ID:SGTC_1065|Compound:prochlorperazine maleate|FD-Score:-3.23|P-value:4.40E-4 ID:SGTC_1067|Compound:clotrimazole|FD-Score:3.77|P-value:5.23E-5 ID:SGTC_1133|Compound:3473-0247|FD-Score:3.17|P-value:5.42E-4 ID:SGTC_1374|Compound:2889-5571|FD-Score:3.27|P-value:3.84E-4 ID:SGTC_1545|Compound:lovastatin|FD-Score:5.00|P-value:1.33E-7 ID:SGTC_1635|Compound:st007035|FD-Score:4.43|P-value:2.57E-6 ID:SGTC_1672|Compound:st016598|FD-Score:5.04|P-value:1.08E-7 ID:SGTC_1673|Compound:st016611|FD-Score:-3.61|P-value:9.81E-5 ID:SGTC_1754|Compound:st042065|FD-Score:16.60|P-value:1.12E-65 ID:SGTC_1757|Compound:st041620|FD-Score:4.23|P-value:6.66E-6 ID:SGTC_1758|Compound:st042942|FD-Score:3.01|P-value:9.76E-4 ID:SGTC_1760|Compound:st045453|FD-Score:31.20|P-value:5.65E-227 ID:SGTC_1762|Compound:st045503|FD-Score:5.61|P-value:3.72E-9 ID:SGTC_1766|Compound:st045414|FD-Score:6.94|P-value:4.30E-13 ID:SGTC_1781|Compound:st038725|FD-Score:4.88|P-value:2.54E-7 ID:SGTC_1801|Compound:st050089|FD-Score:4.59|P-value:1.15E-6 ID:SGTC_1803|Compound:st050068|FD-Score:4.86|P-value:2.81E-7 ID:SGTC_1923|Compound:st058458|FD-Score:3.92|P-value:2.75E-5 ID:SGTC_1986|Compound:st071812|FD-Score:3.22|P-value:4.59E-4 ID:SGTC_2006|Compound:4011487|FD-Score:3.51|P-value:1.52E-4 ID:SGTC_2036|Compound:5160441|FD-Score:4.05|P-value:1.49E-5 ID:SGTC_2060|Compound:5238662|FD-Score:3.50|P-value:1.56E-4 ID:SGTC_2221|Compound:6807166|FD-Score:3.02|P-value:9.37E-4 ID:SGTC_2257|Compound:7960413|FD-Score:3.32|P-value:3.12E-4 ID:SGTC_2441|Compound:5730946|FD-Score:4.05|P-value:1.49E-5 ID:SGTC_2451|Compound:5856223|FD-Score:4.96|P-value:1.61E-7 ID:SGTC_2505|Compound:galangin|FD-Score:3.45|P-value:1.88E-4 ID:SGTC_2567|Compound:aureobasidin a|FD-Score:5.63|P-value:3.39E-9 ID:SGTC_2655|Compound:perillic acid|FD-Score:3.88|P-value:3.16E-5 ID:SGTC_2679|Compound:rapamycin|FD-Score:7.06|P-value:1.84E-13 ID:SGTC_2697|Compound:mebhydrolin|FD-Score:3.78|P-value:5.05E-5 ID:SGTC_2737|Compound:bleomycin|FD-Score:3.69|P-value:7.11E-5 ID:SGTC_2804|Compound:7954240|FD-Score:3.14|P-value:6.08E-4 ID:SGTC_2807|Compound:7945238|FD-Score:7.17|P-value:7.80E-14 ID:SGTC_2808|Compound:7946468|FD-Score:7.56|P-value:3.43E-15 ID:SGTC_2896|Compound:9052656|FD-Score:4.57|P-value:1.28E-6 ID:SGTC_3037|Compound:9091026|FD-Score:3.22|P-value:4.65E-4 ID:SGTC_3152|Compound:9099253|FD-Score:5.30|P-value:2.43E-8

Top fitness defect scores for YHR108W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1760 st045453 31.20 10.4 µM 5.65E-227 NEO1-PIK1 3 4.13E-214
2 SGTC_1754 st042065 16.60 16.1 µM 1.12E-65 NEO1-PIK1 6 4.88E-62
3 SGTC_221 trifluoperazine 16.30 10.3 µM 1.53E-63 NEO1-PIK1 20 5.03E-60
4 SGTC_2808 7946468 7.56 45.5 µM 3.43E-15 2 2.00E-14
5 SGTC_2807 7945238 7.17 52.0 µM 7.80E-14 2 3.81E-13
6 SGTC_2679 rapamycin 7.06 1.0 nM 1.84E-13 56 8.56E-13
7 SGTC_1766 st045414 6.94 44.8 µM 4.30E-13 RPP1 & pyrimidine depletion 4 1.91E-12
8 SGTC_313 3474-0040 6.64 5.0 µM 4.14E-12 NEO1-PIK1 18 1.62E-11
9 SGTC_445 e6 berbamine 6.21 66.0 µM 7.77E-11 NEO1-PIK1 24 2.58E-10
10 SGTC_301 4119-0100 6.12 24.5 µM 1.48E-10 NEO1 38 4.73E-10
11 SGTC_449 xestospongin c 5.67 11.2 µM 2.58E-9 NEO1-PIK1 22 7.03E-9
12 SGTC_2567 aureobasidin a 5.63 60.0 nM 3.39E-9 47 9.09E-9
13 SGTC_1762 st045503 5.61 25.5 µM 3.72E-9 6 9.94E-9
14 SGTC_3152 9099253 5.30 49.5 µM 2.43E-8 NEO1-PIK1 75 5.84E-8
15 SGTC_439 amiodarone 5.18 34.4 µM 4.69E-8 NEO1-PIK1 60 1.09E-7
16 SGTC_1672 st016598 5.04 6.5 µM 1.08E-7 endomembrane recycling 40 2.38E-7
17 SGTC_1043 0916-0009 5.01 19.3 µM 1.24E-7 5 2.72E-7
18 SGTC_1545 lovastatin 5.00 49.4 µM 1.33E-7 8 2.90E-7
19 SGTC_2451 5856223 4.96 45.7 µM 1.61E-7 8 3.48E-7
20 SGTC_1781 st038725 4.88 66.3 µM 2.54E-7 NEO1 76 5.36E-7

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.583 3.73E-305 YML097C VPS9 hom A guanine nucleotide exchange factor involved in vesicle-mediated vacuolar protein transport; specifically stimulates the intrinsic guanine nucleotide exchange activity of Vps21p/Rab5: similar to mammalian ras inhibitors; binds ubiquitin
0.583 2.43E-304 YJL154C VPS35 hom Endosomal subunit of membrane-associated retromer complex required for retrograde transport; receptor that recognizes retrieval signals on cargo proteins, forms subcomplex with Vps26p and Vps29p that selects cargo proteins for retrieval; interacts with Ypt7p
0.518 6.26E-229 YNL267W PIK1 het Phosphatidylinositol 4-kinase; catalyzes first step in the biosynthesis of phosphatidylinositol-4,5-biphosphate; may control cytokinesis through the actin cytoskeleton
0.471 2.02E-185 YLR020C YEH2 hom Steryl ester hydrolase, catalyzes steryl ester hydrolysis at the plasma membrane; involved in sterol metabolism
0.462 2.85E-177 YPR091C NVJ2 hom Lipid-binding ER protein, enriched at nucleus-vacuolar junctions (NVJ); may be involved in sterol metabolism or signaling at the NVJ; contains a synaptotagmin-like-mitochondrial- lipid binding protein (SMP) domain; binds phosphatidylinositols and other lipids in a large-scale study; may interact with ribosomes, based on co-purification experiments
0.443 2.59E-161 YHR012W VPS29 hom Endosomal protein that is a subunit of the membrane-associated retromer complex essential for endosome-to-Golgi retrograde transport; forms a subcomplex with Vps35p and Vps26p that selects cargo proteins for endosome-to-Golgi retrieval
0.442 1.61E-160 YDR084C TVP23 hom Integral membrane protein localized to late Golgi vesicles along with the v-SNARE Tlg2p; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.434 6.25E-154 YBR246W RRT2 hom Protein required for last step of diphthamide biosynthesis; deletion leads to resistance to sordarin and accumulation of diphthine; WD40 domain-containing protein involved in endosomal recycling; forms a complex with Rtt10p that functions in the retromer-mediated pathway for recycling internalized cell-surface proteins; non-essential gene identified in a screen for mutants with increased levels of rDNA transcription
0.426 5.37E-148 YNL183C NPR1 hom Protein kinase that stabilizes several plasma membrane amino acid transporters by antagonizing their ubiquitin-mediated degradation; phosphorylates Aly2p; negatively regulates Ldb19p-mediated endocytosis through phosphorylation of Ldb19p, which prevents its association with the plasma membrane; Npr1p activity is negatively regulated via phosphorylation by the TOR complex
0.425 3.30E-147 YIL048W NEO1 het Putative aminophospholipid translocase (flippase) involved in endocytosis and vacuolar biogenesis; localizes to endosomes and the Golgi aparatus
0.406 4.66E-133 YOR039W CKB2 hom Beta' regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerase
0.401 4.78E-130 YJL053W PEP8 hom Vacuolar protein component of the retromer; forms part of the multimeric membrane-associated retromer complex involved in vacuolar protein sorting along with Vps35p, Vps29p, Vps17p, and Vps5p; essential for endosome-to-Golgi retrograde protein transport; interacts with Ypt7p; protein abundance increases in response to DNA replication stress
0.400 8.98E-129 YGL019W CKB1 hom Beta regulatory subunit of casein kinase 2 (CK2), a Ser/Thr protein kinase with roles in cell growth and proliferation; CK2, comprised of CKA1, CKA2, CKB1 and CKB2, has many substrates including transcription factors and all RNA polymerases
0.395 8.41E-126 YGL199C_d YGL199C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified ORF YIP4/YGL198W
0.394 6.76E-125 YOR357C SNX3 hom Sorting nexin required to maintain late-Golgi resident enzymes in their proper location by recycling molecules from the prevacuolar compartment; contains a PX domain and sequence similarity to human Snx3p