YHR127W

Protein of unknown function; localizes to the nucleus; required for asymmetric localization of Kar9p during mitosis

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR127W / YHR127W

Click on Significant Values for Screen Details ID:SGTC_406|Compound:estrone|FD-Score:3.35|P-value:2.08E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:3.94|P-value:1.63E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.24|P-value:2.96E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:3.30|P-value:2.56E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.36|P-value:1.97E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:5.15|P-value:2.79E-8 ID:SGTC_749|Compound:1000-0129|FD-Score:3.23|P-value:3.30E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:3.06|P-value:6.21E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:3.02|P-value:7.37E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.00|P-value:7.75E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.40|P-value:1.68E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:2.96|P-value:8.99E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.60|P-value:7.44E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.36|P-value:2.08E-6 ID:SGTC_1712|Compound:st032288|FD-Score:4.18|P-value:5.12E-6 ID:SGTC_1715|Compound:st032160|FD-Score:2.97|P-value:8.58E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:4.06|P-value:9.23E-6 ID:SGTC_1724|Compound:st037281|FD-Score:-4.06|P-value:8.45E-6 ID:SGTC_1749|Compound:st037932|FD-Score:3.87|P-value:2.26E-5 ID:SGTC_1896|Compound:st058441|FD-Score:3.61|P-value:7.06E-5 ID:SGTC_1941|Compound:st076221|FD-Score:3.56|P-value:8.79E-5 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.10|P-value:4.93E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.16|P-value:4.29E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.03|P-value:7.11E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-3.22|P-value:3.20E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.42|P-value:1.53E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-2.93|P-value:9.21E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.48|P-value:1.12E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-4.29|P-value:2.74E-6 ID:SGTC_2799|Compound:7812892|FD-Score:5.47|P-value:3.74E-9 ID:SGTC_2835|Compound:9006588|FD-Score:-5.15|P-value:2.41E-8 ID:SGTC_2925|Compound:9010536|FD-Score:-2.98|P-value:7.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.11|P-value:5.30E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.91|P-value:1.87E-5 ID:SGTC_3289|Compound:9119912|FD-Score:-3.26|P-value:2.66E-4 ID:SGTC_3293|Compound:9120939|FD-Score:-3.85|P-value:2.21E-5 ID:SGTC_3298|Compound:9116858|FD-Score:-4.41|P-value:1.49E-6 ID:SGTC_406|Compound:estrone|FD-Score:3.35|P-value:2.08E-4 ID:SGTC_438|Compound:sphingosine|FD-Score:3.94|P-value:1.63E-5 ID:SGTC_473|Compound:2-arachidonoylglycerol|FD-Score:-3.24|P-value:2.96E-4 ID:SGTC_597|Compound:k018-0003|FD-Score:3.30|P-value:2.56E-4 ID:SGTC_699|Compound:0375-0592|FD-Score:3.36|P-value:1.97E-4 ID:SGTC_743|Compound:1275-0778|FD-Score:5.15|P-value:2.79E-8 ID:SGTC_749|Compound:1000-0129|FD-Score:3.23|P-value:3.30E-4 ID:SGTC_844|Compound:0330-0132|FD-Score:3.06|P-value:6.21E-4 ID:SGTC_910|Compound:3454-3003|FD-Score:3.02|P-value:7.37E-4 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.00|P-value:7.75E-4 ID:SGTC_1091|Compound:telmisartan|FD-Score:3.40|P-value:1.68E-4 ID:SGTC_1140|Compound:3229-1848|FD-Score:2.96|P-value:8.99E-4 ID:SGTC_1142|Compound:3253-1408|FD-Score:3.60|P-value:7.44E-5 ID:SGTC_1230|Compound:0170-0365|FD-Score:4.36|P-value:2.08E-6 ID:SGTC_1712|Compound:st032288|FD-Score:4.18|P-value:5.12E-6 ID:SGTC_1715|Compound:st032160|FD-Score:2.97|P-value:8.58E-4 ID:SGTC_1718|Compound:roemerine|FD-Score:4.06|P-value:9.23E-6 ID:SGTC_1724|Compound:st037281|FD-Score:-4.06|P-value:8.45E-6 ID:SGTC_1749|Compound:st037932|FD-Score:3.87|P-value:2.26E-5 ID:SGTC_1896|Compound:st058441|FD-Score:3.61|P-value:7.06E-5 ID:SGTC_1941|Compound:st076221|FD-Score:3.56|P-value:8.79E-5 ID:SGTC_1951|Compound:sophoraflavanone G|FD-Score:-3.10|P-value:4.93E-4 ID:SGTC_1979|Compound:st071809|FD-Score:3.16|P-value:4.29E-4 ID:SGTC_1995|Compound:st072814|FD-Score:3.03|P-value:7.11E-4 ID:SGTC_2351|Compound:9026858|FD-Score:-3.22|P-value:3.20E-4 ID:SGTC_2605|Compound:digitonin|FD-Score:3.42|P-value:1.53E-4 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:-2.93|P-value:9.21E-4 ID:SGTC_2637|Compound:3-alpha-acetoxydihydrodeoxygedunin|FD-Score:-3.48|P-value:1.12E-4 ID:SGTC_2710|Compound:st078562|FD-Score:-4.29|P-value:2.74E-6 ID:SGTC_2799|Compound:7812892|FD-Score:5.47|P-value:3.74E-9 ID:SGTC_2835|Compound:9006588|FD-Score:-5.15|P-value:2.41E-8 ID:SGTC_2925|Compound:9010536|FD-Score:-2.98|P-value:7.71E-4 ID:SGTC_3131|Compound:9127911|FD-Score:3.11|P-value:5.30E-4 ID:SGTC_3283|Compound:9109332|FD-Score:3.91|P-value:1.87E-5 ID:SGTC_3289|Compound:9119912|FD-Score:-3.26|P-value:2.66E-4 ID:SGTC_3293|Compound:9120939|FD-Score:-3.85|P-value:2.21E-5 ID:SGTC_3298|Compound:9116858|FD-Score:-4.41|P-value:1.49E-6

Top fitness defect scores for YHR127W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2799 7812892 5.47 81.8 µM 3.74E-9 superoxide 12 2.19E-8
2 SGTC_743 1275-0778 5.15 158.0 µM 2.79E-8 excess fatty acid 17 1.33E-7
3 SGTC_1230 0170-0365 4.36 3.8 µM 2.08E-6 endomembrane recycling 52 6.44E-6
4 SGTC_1712 st032288 4.18 53.4 µM 5.12E-6 calcium & mitochondrial duress 172 1.45E-5
5 SGTC_1718 roemerine 4.06 11.9 µM 9.23E-6 redox potentiating 51 2.46E-5
6 SGTC_438 sphingosine 3.94 6.7 µM 1.63E-5 22 4.12E-5
7 SGTC_3283 9109332 3.91 35.8 µM 1.87E-5 DNA intercalators 18 4.65E-5
8 SGTC_1749 st037932 3.87 54.2 µM 2.26E-5 30 5.51E-5
9 SGTC_1896 st058441 3.61 89.0 µM 7.06E-5 22 1.54E-4
10 SGTC_1142 3253-1408 3.60 9.8 µM 7.44E-5 20 1.61E-4
11 SGTC_1941 st076221 3.56 61.8 µM 8.79E-5 sphingolipid biosynthesis & PDR1 98 1.88E-4
12 SGTC_2605 digitonin 3.42 300.0 nM 1.53E-4 48 3.10E-4
13 SGTC_1091 telmisartan 3.40 47.6 µM 1.68E-4 35 3.37E-4
14 SGTC_699 0375-0592 3.36 111.0 µM 1.97E-4 iron homeostasis 43 3.89E-4
15 SGTC_406 estrone 3.35 100.0 µM 2.08E-4 22 4.07E-4
16 SGTC_597 k018-0003 3.30 82.8 µM 2.56E-4 RNA pol III & RNase P/MRP 26 4.92E-4
17 SGTC_749 1000-0129 3.23 128.0 µM 3.30E-4 amide catabolism 47 6.19E-4
18 SGTC_1979 st071809 3.16 45.3 µM 4.29E-4 RPP1 & pyrimidine depletion 44 7.83E-4
19 SGTC_3131 9127911 3.11 49.5 µM 5.30E-4 61 9.49E-4
20 SGTC_844 0330-0132 3.06 15.3 µM 6.21E-4 41 0.00109

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 1.90E-19 YDR294C DPL1 hom Dihydrosphingosine phosphate lyase, regulates intracellular levels of sphingolipid long-chain base phosphates (LCBPs), degrades phosphorylated long chain bases, prefers C16 dihydrosphingosine-l-phosphate as a substrate
0.147 1.16E-17 YDR479C PEX29 hom Peroxisomal integral membrane peroxin; involved in the regulation of peroxisomal size, number and distribution; genetic interactions suggest that Pex28p and Pex29p act at steps upstream of those mediated by Pex30p, Pex31p, and Pex32p; forms ER foci upon DNA replication stress
0.137 1.66E-15 YDR282C_p YDR282C_p hom Putative protein of unknown function; localizes to the inner mitochondrial membrane with the C terminus facing the intermembrane space; ortholog of human RMND1, mutation in which is implicated in infantile encephaloneuromyopathy and defective mitochondrial translation
0.128 1.22E-13 YKL051W SFK1 hom Plasma membrane protein that may act together with or upstream of Stt4p to generate normal levels of the essential phospholipid PI4P, at least partially mediates proper localization of Stt4p to the plasma membrane
0.117 8.75E-12 YDR358W GGA1 hom Golgi-localized protein with homology to gamma-adaptin, interacts with and regulates Arf1p and Arf2p in a GTP-dependent manner in order to facilitate traffic through the late Golgi
0.106 8.32E-10 YNL157W IGO1 hom Protein required for initiation of G0 program; prevents degradation of nutrient-regulated mRNAs via the 5'-3' mRNA decay pathway; phosphorylated by Rim15p; GFP protein localizes to the cytoplasm and nucleus; similar to Igo2p
0.105 1.00E-9 YKL063C_p YKL063C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the Golgi
0.104 1.77E-9 YDR376W ARH1 het Oxidoreductase of the mitochondrial inner membrane, involved in cytoplasmic and mitochondrial iron homeostasis and required for activity of Fe-S cluster-containing enzymes; one of the few mitochondrial proteins essential for viability
0.103 2.55E-9 YNR007C ATG3 hom E2-like enzyme involved in autophagy and the cytoplasm-to-vacuole targeting (Cvt) pathway; plays a role in formation of Atg8p-phosphatidylethanolamine conjugates, which are involved in membrane dynamics during autophagy and Cvt; interaction with Atg8p is regulated by its acetylation by Esa1p (catalytic subunit of NuA4 histone acetyltransferase complex) while attenuation of Atg3 acetylation is mediated by histone deacetylase Rpd3p
0.102 2.74E-9 YMR134W ERG29 het Protein of unknown function that may be involved in iron metabolism; mutant bm-8 has a growth defect on iron-limited medium that is complemented by overexpression of Yfh1p; shows localization to the ER; highly conserved in ascomycetes
0.102 2.89E-9 YLR356W ATG33 hom Mitochondrial mitophagy-specific protein; required primarily for mitophagy induced at the post-log phase; not required for other types of selective autophagy or macroautophagy; conserved within fungi, but not in higher eukaryotes
0.102 3.72E-9 YDL224C WHI4 hom Putative RNA binding protein; regulates the cell size requirement for passage through Start and commitment to cell division; WHI4 has a paralog, WHI3, that arose from the whole genome duplication
0.100 7.46E-9 YNL112W DBP2 het ATP-dependent RNA helicase of the DEAD-box protein family; has a strong preference for dsRNA; involved mRNA decay and rRNA processing; may be involved in suppression of transcription from cryptic initiation sites
0.097 1.76E-8 YNL052W COX5A hom Subunit Va of cytochrome c oxidase; cytochrome c oxidase is the terminal member of the mitochondrial inner membrane electron transport chain; Cox5Ap is predominantly expressed during aerobic growth while its isoform Vb (Cox5Bp) is expressed during anaerobic growth; COX5A has a paralog, COX5B, that arose from the whole genome duplication
0.093 6.63E-8 YDR481C PHO8 hom Repressible vacuolar alkaline phosphatase; regulated by levels of Pi and by Pho4p, Pho9p, Pho80p, Pho81p and Pho85p; dephosphorylates phosphotyrosyl peptides; contributes to NAD+ metabolism by producing nicotinamide riboside from NMN