YHR133C / NSG1

Protein involved in regulation of sterol biosynthesis; specifically stabilizes Hmg2p, one of two HMG-CoA isoenzymes that catalyze the rate-limiting step in sterol biosynthesis; homolog of mammalian INSIG proteins; forms foci at the nuclear periphery upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR133C / NSG1

Click on Significant Values for Screen Details ID:SGTC_21|Compound:0849-0113|FD-Score:3.05|P-value:7.16E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:-3.44|P-value:1.68E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.64|P-value:6.91E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:3.07|P-value:6.62E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.89|P-value:2.34E-5 ID:SGTC_277|Compound:nystatin|FD-Score:2.99|P-value:8.82E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:2.97|P-value:9.40E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-2.97|P-value:9.71E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.01|P-value:1.37E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:3.11|P-value:5.77E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.39|P-value:2.01E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.36|P-value:2.29E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.69|P-value:5.72E-5 ID:SGTC_812|Compound:4251-0442|FD-Score:-3.55|P-value:1.07E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:3.90|P-value:2.26E-5 ID:SGTC_873|Compound:0591-4978|FD-Score:3.26|P-value:3.19E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.62|P-value:7.52E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.52|P-value:1.16E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.60|P-value:8.35E-5 ID:SGTC_1519|Compound:esterone|FD-Score:3.21|P-value:3.94E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.64|P-value:6.96E-5 ID:SGTC_1676|Compound:st018489|FD-Score:3.53|P-value:1.08E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.05|P-value:7.41E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-3.92|P-value:2.21E-5 ID:SGTC_1734|Compound:st037168|FD-Score:3.07|P-value:6.56E-4 ID:SGTC_1768|Compound:st044301|FD-Score:-4.93|P-value:1.34E-7 ID:SGTC_1774|Compound:st048371|FD-Score:-3.22|P-value:3.96E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.67|P-value:6.26E-5 ID:SGTC_2024|Compound:5144227|FD-Score:4.06|P-value:1.08E-5 ID:SGTC_2060|Compound:5238662|FD-Score:-3.02|P-value:8.30E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.23|P-value:3.62E-4 ID:SGTC_2244|Compound:6635614|FD-Score:-3.22|P-value:3.88E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.38|P-value:2.05E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-3.69|P-value:5.97E-5 ID:SGTC_2402|Compound:5553605|FD-Score:4.07|P-value:1.02E-5 ID:SGTC_2431|Compound:5490079|FD-Score:-3.00|P-value:8.71E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.70|P-value:5.36E-5 ID:SGTC_2748|Compound:diazoxide|FD-Score:4.51|P-value:1.18E-6 ID:SGTC_2785|Compound:7756463|FD-Score:-3.87|P-value:2.67E-5 ID:SGTC_2871|Compound:9038510|FD-Score:3.59|P-value:8.63E-5 ID:SGTC_3038|Compound:9091290|FD-Score:3.54|P-value:1.04E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.14|P-value:5.10E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.15|P-value:4.84E-4 ID:SGTC_3144|Compound:9087057|FD-Score:5.18|P-value:2.99E-8 ID:SGTC_3204|Compound:9114183|FD-Score:3.42|P-value:1.69E-4 ID:SGTC_3222|Compound:9128301|FD-Score:6.09|P-value:9.26E-11 ID:SGTC_3323|Compound:9138564|FD-Score:3.12|P-value:5.42E-4 ID:SGTC_21|Compound:0849-0113|FD-Score:3.05|P-value:7.16E-4 ID:SGTC_32|Compound:0928-0142|FD-Score:-3.44|P-value:1.68E-4 ID:SGTC_57|Compound:miconazole|FD-Score:3.64|P-value:6.91E-5 ID:SGTC_137|Compound:0438-0332|FD-Score:3.07|P-value:6.62E-4 ID:SGTC_185|Compound:k048-0037|FD-Score:3.89|P-value:2.34E-5 ID:SGTC_277|Compound:nystatin|FD-Score:2.99|P-value:8.82E-4 ID:SGTC_287|Compound:k060-0039|FD-Score:2.97|P-value:9.40E-4 ID:SGTC_392|Compound:N-ethylmaleimide|FD-Score:-2.97|P-value:9.71E-4 ID:SGTC_452|Compound:diindolylmethane|FD-Score:4.01|P-value:1.37E-5 ID:SGTC_533|Compound:1222-0222|FD-Score:3.11|P-value:5.77E-4 ID:SGTC_567|Compound:1080-0542|FD-Score:-3.39|P-value:2.01E-4 ID:SGTC_660|Compound:4031-1742|FD-Score:-3.36|P-value:2.29E-4 ID:SGTC_796|Compound:1234-0020|FD-Score:3.69|P-value:5.72E-5 ID:SGTC_812|Compound:4251-0442|FD-Score:-3.55|P-value:1.07E-4 ID:SGTC_851|Compound:0394-0702|FD-Score:3.90|P-value:2.26E-5 ID:SGTC_873|Compound:0591-4978|FD-Score:3.26|P-value:3.19E-4 ID:SGTC_953|Compound:1188-0045|FD-Score:3.62|P-value:7.52E-5 ID:SGTC_1128|Compound:3448-9350|FD-Score:3.52|P-value:1.16E-4 ID:SGTC_1247|Compound:0448-0043|FD-Score:3.60|P-value:8.35E-5 ID:SGTC_1519|Compound:esterone|FD-Score:3.21|P-value:3.94E-4 ID:SGTC_1607|Compound:st000305|FD-Score:3.64|P-value:6.96E-5 ID:SGTC_1676|Compound:st018489|FD-Score:3.53|P-value:1.08E-4 ID:SGTC_1731|Compound:st035964|FD-Score:-3.05|P-value:7.41E-4 ID:SGTC_1733|Compound:st037357|FD-Score:-3.92|P-value:2.21E-5 ID:SGTC_1734|Compound:st037168|FD-Score:3.07|P-value:6.56E-4 ID:SGTC_1768|Compound:st044301|FD-Score:-4.93|P-value:1.34E-7 ID:SGTC_1774|Compound:st048371|FD-Score:-3.22|P-value:3.96E-4 ID:SGTC_1927|Compound:st060202|FD-Score:3.67|P-value:6.26E-5 ID:SGTC_2024|Compound:5144227|FD-Score:4.06|P-value:1.08E-5 ID:SGTC_2060|Compound:5238662|FD-Score:-3.02|P-value:8.30E-4 ID:SGTC_2166|Compound:5933061|FD-Score:3.23|P-value:3.62E-4 ID:SGTC_2244|Compound:6635614|FD-Score:-3.22|P-value:3.88E-4 ID:SGTC_2331|Compound:7980721|FD-Score:3.38|P-value:2.05E-4 ID:SGTC_2343|Compound:7990716|FD-Score:-3.69|P-value:5.97E-5 ID:SGTC_2402|Compound:5553605|FD-Score:4.07|P-value:1.02E-5 ID:SGTC_2431|Compound:5490079|FD-Score:-3.00|P-value:8.71E-4 ID:SGTC_2441|Compound:5730946|FD-Score:3.70|P-value:5.36E-5 ID:SGTC_2748|Compound:diazoxide|FD-Score:4.51|P-value:1.18E-6 ID:SGTC_2785|Compound:7756463|FD-Score:-3.87|P-value:2.67E-5 ID:SGTC_2871|Compound:9038510|FD-Score:3.59|P-value:8.63E-5 ID:SGTC_3038|Compound:9091290|FD-Score:3.54|P-value:1.04E-4 ID:SGTC_3114|Compound:9122766|FD-Score:3.14|P-value:5.10E-4 ID:SGTC_3139|Compound:9095020|FD-Score:3.15|P-value:4.84E-4 ID:SGTC_3144|Compound:9087057|FD-Score:5.18|P-value:2.99E-8 ID:SGTC_3204|Compound:9114183|FD-Score:3.42|P-value:1.69E-4 ID:SGTC_3222|Compound:9128301|FD-Score:6.09|P-value:9.26E-11 ID:SGTC_3323|Compound:9138564|FD-Score:3.12|P-value:5.42E-4

Top fitness defect scores for YHR133C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature		Screen
Rank
Screen
P-value
1 SGTC_3222 9128301 6.09 49.5 µM 9.26E-11 60S ribosome export 5 5.49E-10
2 SGTC_3144 9087057 5.18 49.5 µM 2.99E-8 4 1.10E-7
3 SGTC_2748 diazoxide 4.51 86.7 µM 1.18E-6 4 3.20E-6
4 SGTC_2402 5553605 4.07 200.0 µM 1.02E-5 22 2.33E-5
5 SGTC_2024 5144227 4.06 134.0 µM 1.08E-5 33 2.45E-5
6 SGTC_452 diindolylmethane 4.01 8.0 µM 1.37E-5 RNA processing & uracil transport 10 3.04E-5
7 SGTC_851 0394-0702 3.90 453.0 µM 2.26E-5 mitochondrial processes 16 4.83E-5
8 SGTC_185 k048-0037 3.89 25.5 µM 2.34E-5 RSC & ERG11 12 5.00E-5
9 SGTC_2441 5730946 3.70 39.1 µM 5.36E-5 27 1.07E-4
10 SGTC_796 1234-0020 3.69 70.7 µM 5.72E-5 SWF1 & branched chain AA biosynthesis 32 1.13E-4
11 SGTC_1927 st060202 3.67 13.8 µM 6.26E-5 27 1.23E-4
12 SGTC_57 miconazole 3.64 162.5 nM 6.91E-5 ergosterol depletion effects on membrane 31 1.35E-4
13 SGTC_1607 st000305 3.64 50.6 µM 6.96E-5 18 1.36E-4
14 SGTC_953 1188-0045 3.62 9.7 µM 7.52E-5 60S ribosome export 35 1.46E-4
15 SGTC_1247 0448-0043 3.60 5.8 µM 8.35E-5 ERG2 64 1.61E-4
16 SGTC_2871 9038510 3.59 13.0 µM 8.63E-5 29 1.66E-4
17 SGTC_3038 9091290 3.54 49.5 µM 1.04E-4 25 1.97E-4
18 SGTC_1676 st018489 3.53 23.4 µM 1.08E-4 60S ribosome export 51 2.04E-4
19 SGTC_1128 3448-9350 3.52 235.0 µM 1.16E-4 35 2.17E-4
20 SGTC_3204 9114183 3.42 49.5 µM 1.69E-4 54 3.08E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.085 9.26E-7 YDR309C GIC2 hom Redundant rho-like GTPase Cdc42p effector; homolog of Gic1p; involved in initiation of budding and cellular polarization; interacts with Cdc42p via the Cdc42/Rac-interactive binding (CRIB) domain and with PI(4,5)P2 via a polybasic region
0.083 1.29E-6 YOR045W TOM6 hom Component of the TOM (translocase of outer membrane) complex responsible for recognition and initial import steps for all mitochondrially directed proteins; promotes assembly and stability of the TOM complex
0.083 1.39E-6 YBR138C_p YBR138C_p hom Cytoplasmic protein of unknown function, potentially phosphorylated by Cdc28p; YBR138C is not an essential gene
0.083 1.46E-6 YDL160C-A MHF2 hom Component of the heterotetrameric MHF histone-fold complex; in humans the MMF complex interacts with both DNA and Mph1p ortholog FANCM to stabilize and remodel blocked replication forks and repair damaged DNA; mhf2 srs2 double mutants are MMS hypersensitive; orthologous to human centromere constitutive-associated network (CCAN) subunit CENP-X, also known as MHF2
0.083 1.67E-6 YKL187C FAT3 hom Protein required for fatty acid uptake; Fat3p abundance increases in cortical patches in response to oleate exposure; the authentic, non-tagged protein is detected in a phosphorylated state in highly purified mitochondria in high-throughput studies
0.078 5.41E-6 YKL162C_p YKL162C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the mitochondrion
0.078 6.26E-6 YKL061W_p BLI1_p hom Putative protein of unknown function; likely member of BLOC complex involved in endosomal cargo sorting; green fluorescent protein (GFP)-fusion protein localizes to the endosome
0.075 1.29E-5 YPR055W SEC8 het Essential 121 kDa subunit of the exocyst complex; the exocyst mediates polarized targeting and tethering of post-Golgi secretory vesicles to active sites of exocytosis at the plasma membrane prior to SNARE-mediated fusion; involved in ER and Golgi inheritance in small buds; relocalizes away from bud neck upon DNA replication stress
0.074 1.59E-5 YOR385W_p YOR385W_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YOR385W is not an essential gene
0.073 2.34E-5 YPL273W SAM4 hom S-adenosylmethionine-homocysteine methyltransferase, functions along with Mht1p in the conversion of S-adenosylmethionine (AdoMet) to methionine to control the methionine/AdoMet ratio
0.072 2.89E-5 YPL156C PRM4 hom Pheromone-regulated protein proposed to be involved in mating; predicted to have 1 transmembrane segment; transcriptionally regulated by Ste12p during mating and by Cat8p during the diauxic shift
0.068 7.57E-5 YPL073C_d YPL073C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps with verified gene UBP16/YPL072W; may interact with ribosome based on co-purification experiments
0.068 8.19E-5 YOR034C AKR2 hom Ankyrin repeat-containing protein; member of a family of putative palmitoyltransferases containing an Asp-His-His-Cys-cysteine rich (DHHC-CRD) domain; possibly involved in constitutive endocytosis of Ste3p; AKR2 has a paralog, AKR1, that arose from the whole genome duplication
0.067 1.12E-4 YCR081W SRB8 hom Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; involved in glucose repression
0.066 1.30E-4 YPR076W_d YPR076W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data