YHR136C / SPL2

Protein with similarity to cyclin-dependent kinase inhibitors; downregulates low-affinity phosphate transport during phosphate limitation; overproduction suppresses a plc1 null mutation; GFP-fusion protein localizes to the cytoplasm

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR136C / SPL2

Click on Significant Values for Screen Details ID:SGTC_89|Compound:0302-0250|FD-Score:4.38|P-value:7.22E-6 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.30|P-value:4.05E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.44|P-value:3.49E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:4.12|P-value:2.32E-5 ID:SGTC_105|Compound:0111-0055|FD-Score:3.90|P-value:5.70E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.10|P-value:8.16E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.31|P-value:5.47E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.30|P-value:5.71E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:5.25|P-value:9.88E-8 ID:SGTC_372|Compound:0155-0182|FD-Score:5.98|P-value:1.45E-9 ID:SGTC_802|Compound:4112-3352|FD-Score:3.76|P-value:1.02E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.38|P-value:3.12E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:-3.30|P-value:4.08E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.22|P-value:7.51E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.37|P-value:4.36E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.65|P-value:1.54E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:5.06|P-value:2.73E-7 ID:SGTC_1976|Compound:st070609|FD-Score:-3.37|P-value:3.20E-4 ID:SGTC_2017|Compound:5132927|FD-Score:3.20|P-value:7.95E-4 ID:SGTC_2133|Compound:5320837|FD-Score:-4.57|P-value:1.97E-6 ID:SGTC_2135|Compound:5326629|FD-Score:4.09|P-value:2.69E-5 ID:SGTC_2136|Compound:5328288|FD-Score:6.43|P-value:8.68E-11 ID:SGTC_2379|Compound:9035213|FD-Score:-3.25|P-value:4.96E-4 ID:SGTC_2457|Compound:5731572|FD-Score:4.32|P-value:9.55E-6 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.96|P-value:4.53E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:6.30|P-value:1.97E-10 ID:SGTC_2591|Compound:skatole|FD-Score:5.21|P-value:1.20E-7 ID:SGTC_2630|Compound:antimycin a|FD-Score:4.71|P-value:1.58E-6 ID:SGTC_2781|Compound:5567276|FD-Score:-3.05|P-value:9.71E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.48|P-value:2.12E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.15|P-value:2.02E-5 ID:SGTC_2806|Compound:7952793|FD-Score:-4.17|P-value:1.24E-5 ID:SGTC_2833|Compound:9001315|FD-Score:4.30|P-value:1.03E-5 ID:SGTC_2873|Compound:9040596|FD-Score:4.49|P-value:4.44E-6 ID:SGTC_2923|Compound:7992654|FD-Score:3.99|P-value:4.08E-5 ID:SGTC_2937|Compound:9044753|FD-Score:7.38|P-value:1.10E-13 ID:SGTC_3065|Compound:9113888|FD-Score:-3.32|P-value:3.84E-4 ID:SGTC_3103|Compound:9120822|FD-Score:3.66|P-value:1.52E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.34|P-value:4.90E-4 ID:SGTC_3156|Compound:9099931|FD-Score:-3.33|P-value:3.67E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.12|P-value:2.32E-5 ID:SGTC_89|Compound:0302-0250|FD-Score:4.38|P-value:7.22E-6 ID:SGTC_95|Compound:0471-0001|FD-Score:-3.30|P-value:4.05E-4 ID:SGTC_97|Compound:0535-0701|FD-Score:3.44|P-value:3.49E-4 ID:SGTC_101|Compound:0490-5701|FD-Score:4.12|P-value:2.32E-5 ID:SGTC_105|Compound:0111-0055|FD-Score:3.90|P-value:5.70E-5 ID:SGTC_128|Compound:0906-2981|FD-Score:-3.10|P-value:8.16E-4 ID:SGTC_141|Compound:0443-0274|FD-Score:3.31|P-value:5.47E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:3.30|P-value:5.71E-4 ID:SGTC_361|Compound:000s-0510|FD-Score:5.25|P-value:9.88E-8 ID:SGTC_372|Compound:0155-0182|FD-Score:5.98|P-value:1.45E-9 ID:SGTC_802|Compound:4112-3352|FD-Score:3.76|P-value:1.02E-4 ID:SGTC_960|Compound:1222-0186|FD-Score:-3.38|P-value:3.12E-4 ID:SGTC_1152|Compound:3909-8331|FD-Score:-3.30|P-value:4.08E-4 ID:SGTC_1265|Compound:0819-1001|FD-Score:3.22|P-value:7.51E-4 ID:SGTC_1430|Compound:4049-0248|FD-Score:3.37|P-value:4.36E-4 ID:SGTC_1568|Compound:digitonin|FD-Score:3.65|P-value:1.54E-4 ID:SGTC_1869|Compound:3',7'-dimethoxyflavone|FD-Score:5.06|P-value:2.73E-7 ID:SGTC_1976|Compound:st070609|FD-Score:-3.37|P-value:3.20E-4 ID:SGTC_2017|Compound:5132927|FD-Score:3.20|P-value:7.95E-4 ID:SGTC_2133|Compound:5320837|FD-Score:-4.57|P-value:1.97E-6 ID:SGTC_2135|Compound:5326629|FD-Score:4.09|P-value:2.69E-5 ID:SGTC_2136|Compound:5328288|FD-Score:6.43|P-value:8.68E-11 ID:SGTC_2379|Compound:9035213|FD-Score:-3.25|P-value:4.96E-4 ID:SGTC_2457|Compound:5731572|FD-Score:4.32|P-value:9.55E-6 ID:SGTC_2552|Compound:juarezic acid|FD-Score:3.96|P-value:4.53E-5 ID:SGTC_2589|Compound:lobaric acid|FD-Score:6.30|P-value:1.97E-10 ID:SGTC_2591|Compound:skatole|FD-Score:5.21|P-value:1.20E-7 ID:SGTC_2630|Compound:antimycin a|FD-Score:4.71|P-value:1.58E-6 ID:SGTC_2781|Compound:5567276|FD-Score:-3.05|P-value:9.71E-4 ID:SGTC_2786|Compound:7743617|FD-Score:-3.48|P-value:2.12E-4 ID:SGTC_2789|Compound:5281112|FD-Score:4.15|P-value:2.02E-5 ID:SGTC_2806|Compound:7952793|FD-Score:-4.17|P-value:1.24E-5 ID:SGTC_2833|Compound:9001315|FD-Score:4.30|P-value:1.03E-5 ID:SGTC_2873|Compound:9040596|FD-Score:4.49|P-value:4.44E-6 ID:SGTC_2923|Compound:7992654|FD-Score:3.99|P-value:4.08E-5 ID:SGTC_2937|Compound:9044753|FD-Score:7.38|P-value:1.10E-13 ID:SGTC_3065|Compound:9113888|FD-Score:-3.32|P-value:3.84E-4 ID:SGTC_3103|Compound:9120822|FD-Score:3.66|P-value:1.52E-4 ID:SGTC_3106|Compound:9121286|FD-Score:3.34|P-value:4.90E-4 ID:SGTC_3156|Compound:9099931|FD-Score:-3.33|P-value:3.67E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.12|P-value:2.32E-5

Top fitness defect scores for YHR136C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2937 9044753 7.38 11.3 µM 1.10E-13 60S ribosome export 2 7.79E-14
2 SGTC_2136 5328288 6.43 199.3 µM 8.68E-11 2 6.40E-11
3 SGTC_2589 lobaric acid 6.30 100.0 µM 1.97E-10 5 1.46E-10
4 SGTC_372 0155-0182 5.98 15.9 µM 1.45E-9 mitochondrial response to ROS 5 1.09E-9
5 SGTC_361 000s-0510 5.25 1.4 µM 9.88E-8 13 7.69E-8
6 SGTC_2591 skatole 5.21 100.0 µM 1.20E-7 9 9.37E-8
7 SGTC_1869 3',7'-dimethoxyflavone 5.06 40.3 µM 2.73E-7 8 2.15E-7
8 SGTC_2630 antimycin a 4.71 100.0 µM 1.58E-6 mitochondrial processes 21 1.26E-6
9 SGTC_2873 9040596 4.49 71.4 µM 4.44E-6 34 3.58E-6
10 SGTC_89 0302-0250 4.38 142.1 µM 7.22E-6 11 5.85E-6
11 SGTC_2457 5731572 4.32 11.7 µM 9.55E-6 14 7.75E-6
12 SGTC_2833 9001315 4.30 45.5 µM 1.03E-5 amide catabolism 43 8.36E-6
13 SGTC_2789 5281112 4.15 71.4 µM 2.02E-5 32 1.65E-5
14 SGTC_101 0490-5701 4.12 117.5 µM 2.32E-5 10 1.90E-5
15 SGTC_3173 9105693 4.12 49.5 µM 2.32E-5 16 1.90E-5
16 SGTC_2135 5326629 4.09 150.4 µM 2.69E-5 RPP1 & pyrimidine depletion 22 2.20E-5
17 SGTC_2923 7992654 3.99 58.1 µM 4.08E-5 Golgi 25 3.36E-5
18 SGTC_2552 juarezic acid 3.96 87.0 µM 4.53E-5 TSC3-RPN4 38 3.74E-5
19 SGTC_105 0111-0055 3.90 734.8 µM 5.70E-5 5 4.72E-5
20 SGTC_802 4112-3352 3.76 742.0 µM 1.02E-4 28 8.50E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.099 9.92E-9 YDR116C MRPL1 hom Mitochondrial ribosomal protein of the large subunit
0.096 2.93E-8 YLR406C RPL31B hom Ribosomal 60S subunit protein L31B; associates with karyopherin Sxm1p; loss of both Rpl31p and Rpl39p confers lethality; homologous to mammalian ribosomal protein L31, no bacterial homolog; RPL31B has a paralog, RPL31A, that arose from the whole genome duplication
0.095 3.11E-8 YNL226W_d YNL226W_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; overlaps the verified gene JJJ1/YNL227C
0.092 9.39E-8 YER085C_p YER085C_p hom Putative protein of unknown function
0.087 4.56E-7 YOR042W CUE5 hom Protein containing a CUE domain that binds ubiquitin, which may facilitate intramolecular monoubiquitination; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm in a punctate pattern
0.080 3.12E-6 YDL212W SHR3 het Endoplasmic reticulum packaging chaperone, required for incorporation of amino acid permeases into COPII coated vesicles for transport to the cell surface
0.078 5.56E-6 YNL247W YNL247W het Cysteinyl-tRNA synthetase; may interact with ribosomes, based on co-purification experiments
0.077 8.95E-6 YBR203W COS111 hom Protein required for resistance to the antifungal drug ciclopirox olamine; not related to the subtelomerically-encoded COS family; the authentic, non-tagged protein is detected in highly purified mitochondria in high-throughput studies
0.076 1.06E-5 YDR221W GTB1 hom Glucosidase II beta subunit, forms a complex with alpha subunit Rot2p; involved in removal of two glucose residues from N-linked glycans during glycoprotein biogenesis in the ER; relocalizes from ER to cytoplasm upon DNA replication stress
0.074 1.66E-5 YJR008W MHO1 hom Protein of unknown function; inhibits haploid invasive growth when overexpressed; synthetically lethal with phospholipase C (PLC1); expression induced by mild heat-stress on a non-fermentable carbon source, upon entry into stationary phase and upon nitrogen deprivation; repressed by inosine and choline in an Opi1p-dependent manner; highly conserved from bacteria to human; Memo, the human homolog, is an ErbB2 interacting protein with an essential function in cell motility
0.074 1.82E-5 YPL250C ICY2 hom Protein of unknown function; mobilized into polysomes upon a shift from a fermentable to nonfermentable carbon source; potential Cdc28p substrate
0.073 2.31E-5 YNR028W CPR8 hom Peptidyl-prolyl cis-trans isomerase (cyclophilin), catalyzes the cis-trans isomerization of peptide bonds N-terminal to proline residues; similarity to Cpr4p suggests a potential role in the secretory pathway
0.073 2.32E-5 YHR073W OSH3 hom Member of an oxysterol-binding protein family with seven members in S. cerevisiae; family members have overlapping, redundant functions in sterol metabolism and collectively perform a function essential for viability
0.073 2.41E-5 YMR117C SPC24 het Component of the evolutionarily conserved kinetochore-associated Ndc80 complex (Ndc80p-Nuf2p-Spc24p-Spc25p); involved in chromosome segregation, spindle checkpoint activity and kinetochore clustering
0.072 3.20E-5 YAL022C FUN26 hom Vacuolar membrane transporter with broad nucleoside selectivity; may regulate balance of nicotinamide riboside (NmR) levels between cytosol and vacuole, contributing to salvage of NmR for use in cytosolic NAD+ synthesis