YHR141C / RPL42B

Ribosomal 60S subunit protein L42B; required for propagation of the killer toxin-encoding M1 double-stranded RNA satellite of the L-A double-stranded RNA virus; homologous to mammalian ribosomal protein L36A, no bacterial homolog; RPL42B has a paralog, RPL42A, that arose from the whole genome duplication

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR141C / RPL42B

Click on Significant Values for Screen Details ID:SGTC_108|Compound:0509-0257|FD-Score:4.25|P-value:4.83E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:4.21|P-value:5.66E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:3.47|P-value:7.33E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.46|P-value:2.11E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-5.14|P-value:9.65E-7 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.54|P-value:5.93E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.71|P-value:3.39E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.47|P-value:7.30E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:7.39|P-value:5.40E-12 ID:SGTC_354|Compound:2783-3500|FD-Score:3.50|P-value:6.79E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.33|P-value:4.87E-7 ID:SGTC_529|Compound:4076-0302|FD-Score:-4.34|P-value:2.86E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:4.17|P-value:6.40E-5 ID:SGTC_590|Compound:k048-0076|FD-Score:3.45|P-value:7.84E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:3.46|P-value:7.58E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:5.35|P-value:4.39E-7 ID:SGTC_916|Compound:4466-0038|FD-Score:4.17|P-value:6.57E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.45|P-value:7.94E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.68|P-value:3.69E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.51|P-value:1.75E-5 ID:SGTC_1726|Compound:st035556|FD-Score:5.19|P-value:9.32E-7 ID:SGTC_1933|Compound:st074711|FD-Score:3.79|P-value:2.59E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.63|P-value:4.31E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.56|P-value:5.51E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.43|P-value:8.26E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-4.88|P-value:3.04E-6 ID:SGTC_2507|Compound:purpurin|FD-Score:4.93|P-value:2.95E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:5.97|P-value:2.07E-8 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.71|P-value:2.86E-4 ID:SGTC_2851|Compound:9015922|FD-Score:-3.72|P-value:2.73E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.84|P-value:1.82E-4 ID:SGTC_2893|Compound:9048488|FD-Score:4.73|P-value:7.16E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-5.77|P-value:4.44E-8 ID:SGTC_3124|Compound:9127567|FD-Score:4.68|P-value:8.65E-6 ID:SGTC_3148|Compound:9097893|FD-Score:-6.52|P-value:7.61E-10 ID:SGTC_3154|Compound:9100113|FD-Score:-7.22|P-value:1.15E-11 ID:SGTC_3158|Compound:9100007|FD-Score:-4.08|P-value:7.60E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-7.31|P-value:6.37E-12 ID:SGTC_3219|Compound:9131112|FD-Score:-4.99|P-value:1.83E-6 ID:SGTC_3228|Compound:9131799|FD-Score:-3.78|P-value:2.27E-4 ID:SGTC_3350|Compound:9150499|FD-Score:4.16|P-value:6.86E-5 ID:SGTC_3356|Compound:9151811|FD-Score:3.39|P-value:9.37E-4 ID:SGTC_108|Compound:0509-0257|FD-Score:4.25|P-value:4.83E-5 ID:SGTC_116|Compound:0155-0182|FD-Score:4.21|P-value:5.66E-5 ID:SGTC_138|Compound:0850-0259|FD-Score:3.47|P-value:7.33E-4 ID:SGTC_149|Compound:3970-0822|FD-Score:4.46|P-value:2.11E-5 ID:SGTC_217|Compound:ketoconazole|FD-Score:-5.14|P-value:9.65E-7 ID:SGTC_240|Compound:5hr heat shock (37°C) + Streptovitacin|FD-Score:3.54|P-value:5.93E-4 ID:SGTC_242|Compound:5hr heat shock (37°C) + 5-fluorouracil|FD-Score:3.71|P-value:3.39E-4 ID:SGTC_243|Compound:5hr heat shock (37°C) + splitomicin|FD-Score:3.47|P-value:7.30E-4 ID:SGTC_351|Compound:caspofungin|FD-Score:7.39|P-value:5.40E-12 ID:SGTC_354|Compound:2783-3500|FD-Score:3.50|P-value:6.79E-4 ID:SGTC_514|Compound:4466-0038|FD-Score:5.33|P-value:4.87E-7 ID:SGTC_529|Compound:4076-0302|FD-Score:-4.34|P-value:2.86E-5 ID:SGTC_539|Compound:4859-0462|FD-Score:4.17|P-value:6.40E-5 ID:SGTC_590|Compound:k048-0076|FD-Score:3.45|P-value:7.84E-4 ID:SGTC_600|Compound:1495-0418|FD-Score:3.46|P-value:7.58E-4 ID:SGTC_902|Compound:1120-0002|FD-Score:5.35|P-value:4.39E-7 ID:SGTC_916|Compound:4466-0038|FD-Score:4.17|P-value:6.57E-5 ID:SGTC_1090|Compound:efavirenz|FD-Score:3.45|P-value:7.94E-4 ID:SGTC_1101|Compound:ebselen|FD-Score:3.68|P-value:3.69E-4 ID:SGTC_1105|Compound:epirubicin|FD-Score:4.51|P-value:1.75E-5 ID:SGTC_1726|Compound:st035556|FD-Score:5.19|P-value:9.32E-7 ID:SGTC_1933|Compound:st074711|FD-Score:3.79|P-value:2.59E-4 ID:SGTC_1964|Compound:st076914|FD-Score:3.63|P-value:4.31E-4 ID:SGTC_2066|Compound:5222451|FD-Score:3.56|P-value:5.51E-4 ID:SGTC_2090|Compound:5301565|FD-Score:3.43|P-value:8.26E-4 ID:SGTC_2286|Compound:7945697|FD-Score:-4.88|P-value:3.04E-6 ID:SGTC_2507|Compound:purpurin|FD-Score:4.93|P-value:2.95E-6 ID:SGTC_2619|Compound:obliquin|FD-Score:5.97|P-value:2.07E-8 ID:SGTC_2761|Compound:levamisole|FD-Score:-3.71|P-value:2.86E-4 ID:SGTC_2851|Compound:9015922|FD-Score:-3.72|P-value:2.73E-4 ID:SGTC_2874|Compound:9043904|FD-Score:-3.84|P-value:1.82E-4 ID:SGTC_2893|Compound:9048488|FD-Score:4.73|P-value:7.16E-6 ID:SGTC_2896|Compound:9052656|FD-Score:-5.77|P-value:4.44E-8 ID:SGTC_3124|Compound:9127567|FD-Score:4.68|P-value:8.65E-6 ID:SGTC_3148|Compound:9097893|FD-Score:-6.52|P-value:7.61E-10 ID:SGTC_3154|Compound:9100113|FD-Score:-7.22|P-value:1.15E-11 ID:SGTC_3158|Compound:9100007|FD-Score:-4.08|P-value:7.60E-5 ID:SGTC_3211|Compound:9128781|FD-Score:-7.31|P-value:6.37E-12 ID:SGTC_3219|Compound:9131112|FD-Score:-4.99|P-value:1.83E-6 ID:SGTC_3228|Compound:9131799|FD-Score:-3.78|P-value:2.27E-4 ID:SGTC_3350|Compound:9150499|FD-Score:4.16|P-value:6.86E-5 ID:SGTC_3356|Compound:9151811|FD-Score:3.39|P-value:9.37E-4

Top fitness defect scores for YHR141C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_351 caspofungin 7.39 25.0 nM 5.40E-12 cell wall 21 7.46E-14
2 SGTC_2619 obliquin 5.97 87.9 µM 2.07E-8 cell wall 77 1.20E-9
3 SGTC_902 1120-0002 5.35 2.1 µM 4.39E-7 azole & statin 23 4.34E-8
4 SGTC_514 4466-0038 5.33 2.6 nM 4.87E-7 cell wall 96 4.91E-8
5 SGTC_1726 st035556 5.19 14.6 µM 9.32E-7 17 1.05E-7
6 SGTC_2507 purpurin 4.93 79.7 µM 2.95E-6 copper-dependent oxidative stress 20 4.05E-7
7 SGTC_2893 9048488 4.73 26.0 µM 7.16E-6 fatty acid desaturase (OLE1) 12 1.15E-6
8 SGTC_3124 9127567 4.68 49.5 µM 8.65E-6 DNA damage response 10 1.43E-6
9 SGTC_1105 epirubicin 4.51 18.1 µM 1.75E-5 anthracycline transcription coupled DNA repair 11 3.27E-6
10 SGTC_149 3970-0822 4.46 46.1 µM 2.11E-5 tubulin folding & SWR complex 68 4.06E-6
11 SGTC_108 0509-0257 4.25 621.1 µM 4.83E-5 40 1.07E-5
12 SGTC_116 0155-0182 4.21 31.8 µM 5.66E-5 mitochondrial response to ROS 63 1.29E-5
13 SGTC_539 4859-0462 4.17 2.5 µM 6.40E-5 RNA pol III & RNase P/MRP 20 1.49E-5
14 SGTC_916 4466-0038 4.17 42.0 nM 6.57E-5 cell wall 60 1.54E-5
15 SGTC_3350 9150499 4.16 3.6 µM 6.86E-5 cell wall 49 1.62E-5
16 SGTC_1933 st074711 3.79 15.9 µM 2.59E-4 48 7.66E-5
17 SGTC_242 5hr heat shock (37°C) + 5-fluorouracil 3.71 4.6 µM 3.39E-4 heat shock/prefoldin 44 1.05E-4
18 SGTC_1101 ebselen 3.68 5.3 µM 3.69E-4 32 1.16E-4
19 SGTC_1964 st076914 3.63 61.0 µM 4.31E-4 RPP1 & pyrimidine depletion 27 1.39E-4
20 SGTC_2066 5222451 3.56 176.0 µM 5.51E-4 heme biosynthesis & mitochondrial translocase 46 1.85E-4

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.256 1.77E-51 YGL033W HOP2 hom Meiosis-specific protein that localizes to chromosomes, preventing synapsis between nonhomologous chromosomes and ensuring synapsis between homologs; complexes with Mnd1p to promote homolog pairing and meiotic double-strand break repair
0.233 1.08E-42 YJL159W HSP150 hom O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication
0.218 2.98E-37 YOR091W TMA46 hom Protein of unknown function that associates with translating ribosomes; interacts with GTPase Rbg1p
0.208 3.74E-34 YBR020W GAL1 hom Galactokinase; phosphorylates alpha-D-galactose to alpha-D-galactose-1-phosphate in the first step of galactose catabolism; expression regulated by Gal4p; GAL1 has a paralog, GAL3, that arose from the whole genome duplication
0.199 2.02E-31 YDR540C IRC4 hom Putative protein of unknown function; null mutant displays increased levels of spontaneous Rad52p foci; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm and nucleus
0.197 9.37E-31 YOR051C ETT1 hom Nuclear protein that inhibits replication of Brome mosaic virus in S. cerevisiae, which is a model system for studying replication of positive-strand RNA viruses in their natural hosts; deletion increases stop codon readthrough
0.190 1.01E-28 YMR124W_p YMR124W_p hom Protein of unknown function; GFP-fusion protein localizes to the cell periphery, cytoplasm, bud, and bud neck; interacts with Crm1p in two-hybrid assay; YMR124W is not an essential gene; predicted to have a role in organelle organization
0.189 2.03E-28 YMR121C RPL15B hom Ribosomal 60S subunit protein L15B; binds to 5.8 S rRNA; homologous to mammalian ribosomal protein L15, no bacterial homolog; RPL15B has a paralog, RPL15A, that arose from the whole genome duplication; relocalizes from nucleus to nucleolus upon DNA replication stress
0.186 1.96E-27 YPR061C JID1 hom Probable Hsp40p co-chaperone, has a DnaJ-like domain and appears to be involved in ER-associated degradation of misfolded proteins containing a tightly folded cytoplasmic domain; inhibits replication of Brome mosaic virus in S. cerevisiae
0.181 4.72E-26 YHR207C SET5 hom Methyltransferase involved in methylation of histone H4 Lys5, -8, -12; S-adenosylmethionine-dependent; zinc-finger protein, contains one canonical and two unusual fingers in unusual arrangements; deletion enhances replication of positive-strand RNA virus
0.173 6.35E-24 YLL032C YLL032C hom Protein of unknown function that may interact with ribosomes, based on co-purification experiments; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm; YLL032C is not an essential gene
0.171 2.14E-23 YOL003C PFA4 hom Palmitoyltransferase with autoacylation activity, required for palmitoylation of amino acid permeases containing a C-terminal Phe-Trp-Cys site; required for modification of Chs3p; member of the DHHC family of putative palmitoyltransferases
0.171 2.34E-23 YDR260C SWM1 hom Subunit of the anaphase-promoting complex, which is an E3 ubiquitin ligase that regulates the metaphase-anaphase transition and exit from mitosis; required for activation of the daughter-specific gene expression and spore wall maturation
0.170 3.67E-23 YPL066W RGL1 hom Regulator of Rho1p signaling, cofactor of Tus1p; required for the localization of Tus1p during all phases of cytokinesis; green fluorescent protein (GFP)-fusion protein localizes to the bud neck and cytoplasm; null mutant is viable and exhibits growth defect on a non-fermentable (respiratory) carbon source
0.160 1.38E-20 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan