YHR142W / CHS7

Protein of unknown function; may be involved in chitin biosynthesis by regulation of Chs3p export from the ER; relocalizes from bud neck to ER upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR142W / CHS7

Click on Significant Values for Screen Details ID:SGTC_78|Compound:0336-0123|FD-Score:4.30|P-value:4.43E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-3.44|P-value:5.10E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.41|P-value:3.13E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:4.98|P-value:4.60E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:-3.69|P-value:2.34E-4 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:4.24|P-value:5.30E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.92|P-value:5.79E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.72|P-value:2.15E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:4.70|P-value:1.22E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:5.54|P-value:5.84E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:4.71|P-value:1.19E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.19|P-value:6.19E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:4.34|P-value:3.82E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:4.87|P-value:6.94E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:5.40|P-value:9.96E-6 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.54|P-value:5.65E-6 ID:SGTC_1419|Compound:3993-2473|FD-Score:4.30|P-value:4.40E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:-3.58|P-value:3.29E-4 ID:SGTC_1531|Compound:st059297|FD-Score:4.12|P-value:7.56E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.57|P-value:1.86E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:5.05|P-value:3.57E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:4.05|P-value:9.37E-4 ID:SGTC_1623|Compound:st003709|FD-Score:4.62|P-value:1.60E-4 ID:SGTC_1644|Compound:st010134|FD-Score:4.72|P-value:1.15E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-3.22|P-value:9.79E-4 ID:SGTC_1704|Compound:st028805|FD-Score:5.51|P-value:6.51E-6 ID:SGTC_1729|Compound:st035615|FD-Score:5.34|P-value:1.25E-5 ID:SGTC_1831|Compound:st055328|FD-Score:4.05|P-value:9.19E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-3.41|P-value:5.55E-4 ID:SGTC_1856|Compound:st057054|FD-Score:4.12|P-value:7.55E-4 ID:SGTC_2030|Compound:5175110|FD-Score:4.61|P-value:1.64E-4 ID:SGTC_2034|Compound:5197015|FD-Score:5.56|P-value:5.36E-6 ID:SGTC_2081|Compound:5214970|FD-Score:5.25|P-value:1.75E-5 ID:SGTC_2137|Compound:5261020|FD-Score:-3.42|P-value:5.45E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.09|P-value:8.25E-4 ID:SGTC_2202|Compound:7226349|FD-Score:4.24|P-value:5.32E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.60|P-value:1.67E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.36|P-value:6.48E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.04|P-value:9.38E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-5.64|P-value:1.20E-7 ID:SGTC_2322|Compound:7413319|FD-Score:5.34|P-value:1.23E-5 ID:SGTC_2327|Compound:6189656|FD-Score:5.09|P-value:3.20E-5 ID:SGTC_2399|Compound:5884133|FD-Score:5.54|P-value:5.85E-6 ID:SGTC_2400|Compound:5203494|FD-Score:4.58|P-value:1.84E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.40|P-value:3.17E-4 ID:SGTC_2481|Compound:5478948|FD-Score:4.28|P-value:4.65E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:-3.49|P-value:4.43E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.79|P-value:1.73E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:5.47|P-value:7.45E-6 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.18|P-value:6.34E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.04|P-value:3.70E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-5.77|P-value:6.85E-8 ID:SGTC_2852|Compound:9023854|FD-Score:5.60|P-value:4.63E-6 ID:SGTC_2881|Compound:9058953|FD-Score:4.13|P-value:7.22E-4 ID:SGTC_2912|Compound:7964298|FD-Score:4.41|P-value:3.11E-4 ID:SGTC_2933|Compound:9026794|FD-Score:4.48|P-value:2.51E-4 ID:SGTC_2945|Compound:9067681|FD-Score:4.44|P-value:2.82E-4 ID:SGTC_2951|Compound:9072950|FD-Score:4.55|P-value:2.01E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.69|P-value:2.38E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.65|P-value:1.43E-4 ID:SGTC_3035|Compound:9091303|FD-Score:4.47|P-value:2.58E-4 ID:SGTC_3148|Compound:9097893|FD-Score:4.43|P-value:2.91E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.60|P-value:1.70E-4 ID:SGTC_3236|Compound:9132701|FD-Score:5.84|P-value:1.71E-6 ID:SGTC_3238|Compound:9133470|FD-Score:-3.75|P-value:1.95E-4 ID:SGTC_78|Compound:0336-0123|FD-Score:4.30|P-value:4.43E-4 ID:SGTC_109|Compound:0132-0036|FD-Score:-3.44|P-value:5.10E-4 ID:SGTC_238|Compound:5hr heat shock (37°C) + nsc-638432|FD-Score:4.41|P-value:3.13E-4 ID:SGTC_360|Compound:0957-0399|FD-Score:4.98|P-value:4.60E-5 ID:SGTC_363|Compound:0455-0021|FD-Score:-3.69|P-value:2.34E-4 ID:SGTC_478|Compound:N-arachidonylglycine|FD-Score:4.24|P-value:5.30E-4 ID:SGTC_511|Compound:blebbistatin|FD-Score:4.92|P-value:5.79E-5 ID:SGTC_669|Compound:0108-0021|FD-Score:-3.72|P-value:2.15E-4 ID:SGTC_758|Compound:0868-0029|FD-Score:4.70|P-value:1.22E-4 ID:SGTC_759|Compound:3486-0358|FD-Score:5.54|P-value:5.84E-6 ID:SGTC_768|Compound:0384-0033|FD-Score:4.71|P-value:1.19E-4 ID:SGTC_819|Compound:1013-0258|FD-Score:4.19|P-value:6.19E-4 ID:SGTC_863|Compound:0466-0191|FD-Score:4.34|P-value:3.82E-4 ID:SGTC_954|Compound:1189-0853|FD-Score:4.87|P-value:6.94E-5 ID:SGTC_964|Compound:1287-0107|FD-Score:5.40|P-value:9.96E-6 ID:SGTC_1133|Compound:3473-0247|FD-Score:5.54|P-value:5.65E-6 ID:SGTC_1419|Compound:3993-2473|FD-Score:4.30|P-value:4.40E-4 ID:SGTC_1475|Compound:4092-1048|FD-Score:-3.58|P-value:3.29E-4 ID:SGTC_1531|Compound:st059297|FD-Score:4.12|P-value:7.56E-4 ID:SGTC_1547|Compound:harmine|FD-Score:4.57|P-value:1.86E-4 ID:SGTC_1565|Compound:sinapyl alcohol|FD-Score:5.05|P-value:3.57E-5 ID:SGTC_1578|Compound:josamycin|FD-Score:4.05|P-value:9.37E-4 ID:SGTC_1623|Compound:st003709|FD-Score:4.62|P-value:1.60E-4 ID:SGTC_1644|Compound:st010134|FD-Score:4.72|P-value:1.15E-4 ID:SGTC_1673|Compound:st016611|FD-Score:-3.22|P-value:9.79E-4 ID:SGTC_1704|Compound:st028805|FD-Score:5.51|P-value:6.51E-6 ID:SGTC_1729|Compound:st035615|FD-Score:5.34|P-value:1.25E-5 ID:SGTC_1831|Compound:st055328|FD-Score:4.05|P-value:9.19E-4 ID:SGTC_1837|Compound:st055472|FD-Score:-3.41|P-value:5.55E-4 ID:SGTC_1856|Compound:st057054|FD-Score:4.12|P-value:7.55E-4 ID:SGTC_2030|Compound:5175110|FD-Score:4.61|P-value:1.64E-4 ID:SGTC_2034|Compound:5197015|FD-Score:5.56|P-value:5.36E-6 ID:SGTC_2081|Compound:5214970|FD-Score:5.25|P-value:1.75E-5 ID:SGTC_2137|Compound:5261020|FD-Score:-3.42|P-value:5.45E-4 ID:SGTC_2200|Compound:6600604|FD-Score:4.09|P-value:8.25E-4 ID:SGTC_2202|Compound:7226349|FD-Score:4.24|P-value:5.32E-4 ID:SGTC_2268|Compound:7933382|FD-Score:4.60|P-value:1.67E-4 ID:SGTC_2276|Compound:7947513|FD-Score:-3.36|P-value:6.48E-4 ID:SGTC_2316|Compound:5844804|FD-Score:4.04|P-value:9.38E-4 ID:SGTC_2319|Compound:6033012|FD-Score:-5.64|P-value:1.20E-7 ID:SGTC_2322|Compound:7413319|FD-Score:5.34|P-value:1.23E-5 ID:SGTC_2327|Compound:6189656|FD-Score:5.09|P-value:3.20E-5 ID:SGTC_2399|Compound:5884133|FD-Score:5.54|P-value:5.85E-6 ID:SGTC_2400|Compound:5203494|FD-Score:4.58|P-value:1.84E-4 ID:SGTC_2426|Compound:5582046|FD-Score:4.40|P-value:3.17E-4 ID:SGTC_2481|Compound:5478948|FD-Score:4.28|P-value:4.65E-4 ID:SGTC_2533|Compound:agelasine|FD-Score:-3.49|P-value:4.43E-4 ID:SGTC_2539|Compound:epi(13)torulosol|FD-Score:-3.79|P-value:1.73E-4 ID:SGTC_2569|Compound:tetrandrine|FD-Score:5.47|P-value:7.45E-6 ID:SGTC_2617|Compound:dihydrotanshinone i|FD-Score:4.18|P-value:6.34E-4 ID:SGTC_2629|Compound:bisabolol|FD-Score:5.04|P-value:3.70E-5 ID:SGTC_2640|Compound:pentamidine|FD-Score:-5.77|P-value:6.85E-8 ID:SGTC_2852|Compound:9023854|FD-Score:5.60|P-value:4.63E-6 ID:SGTC_2881|Compound:9058953|FD-Score:4.13|P-value:7.22E-4 ID:SGTC_2912|Compound:7964298|FD-Score:4.41|P-value:3.11E-4 ID:SGTC_2933|Compound:9026794|FD-Score:4.48|P-value:2.51E-4 ID:SGTC_2945|Compound:9067681|FD-Score:4.44|P-value:2.82E-4 ID:SGTC_2951|Compound:9072950|FD-Score:4.55|P-value:2.01E-4 ID:SGTC_2989|Compound:9073649|FD-Score:-3.69|P-value:2.38E-4 ID:SGTC_2993|Compound:9054257|FD-Score:4.65|P-value:1.43E-4 ID:SGTC_3035|Compound:9091303|FD-Score:4.47|P-value:2.58E-4 ID:SGTC_3148|Compound:9097893|FD-Score:4.43|P-value:2.91E-4 ID:SGTC_3173|Compound:9105693|FD-Score:4.60|P-value:1.70E-4 ID:SGTC_3236|Compound:9132701|FD-Score:5.84|P-value:1.71E-6 ID:SGTC_3238|Compound:9133470|FD-Score:-3.75|P-value:1.95E-4

Top fitness defect scores for YHR142W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_3236 9132701 5.84 49.5 µM 1.71E-6 TSC3-RPN4 5 2.58E-9
2 SGTC_2852 9023854 5.60 52.0 µM 4.63E-6 13 1.10E-8
3 SGTC_2034 5197015 5.56 53.1 µM 5.36E-6 SWF1 & branched chain AA biosynthesis 10 1.36E-8
4 SGTC_1133 3473-0247 5.54 50.0 µM 5.65E-6 RSC complex & mRNA processing 11 1.47E-8
5 SGTC_759 3486-0358 5.54 89.3 µM 5.84E-6 5 1.54E-8
6 SGTC_2399 5884133 5.54 200.0 µM 5.85E-6 cell wall signaling 34 1.54E-8
7 SGTC_1704 st028805 5.51 70.8 µM 6.51E-6 1 1.80E-8
8 SGTC_2569 tetrandrine 5.47 100.0 µM 7.45E-6 6 2.20E-8
9 SGTC_964 1287-0107 5.40 19.4 µM 9.96E-6 cell wall 17 3.35E-8
10 SGTC_2322 7413319 5.34 38.2 µM 1.23E-5 ERAD & cell cycle 80 4.57E-8
11 SGTC_1729 st035615 5.34 46.0 µM 1.25E-5 12 4.65E-8
12 SGTC_2081 5214970 5.25 32.3 µM 1.75E-5 6 7.65E-8
13 SGTC_2327 6189656 5.09 200.0 µM 3.20E-5 7 1.84E-7
14 SGTC_1565 sinapyl alcohol 5.05 95.1 µM 3.57E-5 6 2.16E-7
15 SGTC_2629 bisabolol 5.04 100.0 µM 3.70E-5 8 2.28E-7
16 SGTC_360 0957-0399 4.98 1.0 µM 4.60E-5 cell wall 23 3.12E-7
17 SGTC_511 blebbistatin 4.92 171.0 µM 5.79E-5 DNA intercalators 9 4.38E-7
18 SGTC_954 1189-0853 4.87 2.9 µM 6.94E-5 endomembrane recycling 21 5.71E-7
19 SGTC_1644 st010134 4.72 20.9 µM 1.15E-4 mitochondrial processes 5 1.20E-6
20 SGTC_768 0384-0033 4.71 77.3 µM 1.19E-4 fatty acid desaturase (OLE1) 58 1.25E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.155 1.85E-19 YBR023C CHS3 hom Chitin synthase III, catalyzes the transfer of N-acetylglucosamine (GlcNAc) to chitin; required for synthesis of the majority of cell wall chitin, the chitin ring during bud emergence, and spore wall chitosan
0.119 4.28E-12 YDR348C PAL1 hom Protein of unknown function thought to be involved in endocytosis; Pal1p physically interacts with Ede1p and is found at endocytic sites at the cell periphery during the early stages of endocytosis; green fluorescent protein (GFP)-fusion protein also localizes to the bud neck; potential Cdc28p substrate; similar to the S. pombe Pal1 protein; relocalizes from bud neck to cytoplasm upon DNA replication stress
0.119 5.15E-12 YDR162C NBP2 hom Protein involved in the HOG (high osmolarity glycerol) pathway; negatively regulates Hog1p by recruitment of phosphatase Ptc1p the Pbs2p-Hog1p complex; interacts with Bck1p and down regulates the cell wall integrity pathway; found in the nucleus and cytoplasm, contains an SH3 domain and a Ptc1p binding domain (PBM)
0.111 1.45E-10 YJL099W CHS6 hom Member of the ChAPs (Chs5p-Arf1p-binding proteins) family; part of the exomer complex that mediates export of specific cargo proteins, including Chs3p, from the Golgi to the plasma membrane; CHS6 has a paralog, BCH2, that arose from the whole genome duplication
0.104 1.84E-9 YBL061C SKT5 hom Activator of Chs3p (chitin synthase III) during vegetative growth; recruits Chs3p to the bud neck via interaction with Bni4p; SKT5 has a paralog, SHC1, that arose from the whole genome duplication
0.098 1.16E-8 YLL043W FPS1 hom Plasma membrane channel, member of major intrinsic protein (MIP) family; involved in efflux of glycerol and in uptake of acetic acid and the trivalent metalloids arsenite and antimonite; phosphorylated by Hog1p MAPK under acetate stress
0.091 1.44E-7 YHR030C SLT2 hom Serine/threonine MAP kinase; involved in regulating maintenance of cell wall integrity, progression through the cell cycle, and nuclear mRNA retention in heat shock; required for mitophagy and pexophagy; affects recruitment of mitochondria to the phagophore assembly site (PAS); regulated by the PKC1-mediated signaling pathway
0.091 1.42E-7 YPR165W RHO1 het GTP-binding protein of the rho subfamily of Ras-like proteins, involved in establishment of cell polarity; regulates protein kinase C (Pkc1p) and the cell wall synthesizing enzyme 1,3-beta-glucan synthase (Fks1p and Gsc2p)
0.089 2.22E-7 YOR008C SLG1 hom Sensor-transducer of the stress-activated PKC1-MPK1 kinase pathway; involved in maintenance of cell wall integrity; required for mitophagy; involved in organization of the actin cytoskeleton; secretory pathway Wsc1p is required for the arrest of secretion response
0.089 2.81E-7 YFL025C BST1 hom GPI inositol deacylase of the ER that negatively regulates COPII vesicle formation, prevents production of vesicles with defective subunits, required for proper discrimination between resident ER proteins and Golgi-bound cargo molecules
0.087 4.94E-7 YEL044W IES6 hom Protein that associates with the INO80 chromatin remodeling complex under low-salt conditions; human ortholog INO80C is a member of the human INO80 complex; implicated in DNA repair based on genetic interactions with RAD52 epistasis genes
0.084 1.14E-6 YPL065W VPS28 hom Component of the ESCRT-I complex (Stp22p, Srn2p, Vps28p, and Mvb12p), which is involved in ubiquitin-dependent sorting of proteins into the endosome; conserved C-terminal domain interacts with ESCRT-III subunit Vps20p
0.083 1.52E-6 YLR331C_d JIP3_d hom Dubious open reading frame, unlikely to encode a protein; not conserved in closely related Saccharomyces species; 98% of ORF overlaps the verified gene MID2
0.083 1.62E-6 YDR389W SAC7 hom GTPase activating protein (GAP) for Rho1p, involved in signaling to the actin cytoskeleton, null mutations suppress tor2 mutations and temperature sensitive mutations in actin; potential Cdc28p substrate
0.082 1.86E-6 YLR330W CHS5 hom Component of the exomer complex, which also contains Csh6p, Bch1p, Bch2p, and Bud7p and is involved in export of selected proteins, such as chitin synthase Chs3p, from the Golgi to the plasma membrane