YHR154W / RTT107

Protein implicated in Mms22-dependent DNA repair during S phase; involved in recruiting the SMC5/6 complex to double-strand breaks; DNA damage induces phosphorylation by Mec1p at one or more SQ/TQ motifs; interacts with Mms22p and Slx4p; has four BRCT domains; has a role in regulation of Ty1 transposition; relative distribution to nuclear foci increases upon DNA replication stress

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR154W / RTT107

Click on Significant Values for Screen Details ID:SGTC_53|Compound:0973-0023|FD-Score:3.47|P-value:9.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.24|P-value:1.00E-8 ID:SGTC_92|Compound:0063-0024|FD-Score:4.85|P-value:6.56E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:3.84|P-value:2.88E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.23|P-value:1.33E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:3.68|P-value:4.85E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.83|P-value:7.33E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.64|P-value:5.57E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:3.93|P-value:2.15E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:-4.41|P-value:3.20E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:3.48|P-value:9.04E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:4.47|P-value:3.06E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:3.58|P-value:6.58E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.51|P-value:8.28E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:4.11|P-value:1.15E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.49|P-value:8.84E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.50|P-value:8.58E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:4.21|P-value:7.94E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.67|P-value:1.40E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.52|P-value:8.12E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.71|P-value:4.36E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:5.46|P-value:4.65E-7 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.49|P-value:8.90E-4 ID:SGTC_1636|Compound:st007707|FD-Score:4.62|P-value:1.67E-5 ID:SGTC_1673|Compound:st016611|FD-Score:-3.71|P-value:3.89E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-4.29|P-value:5.20E-5 ID:SGTC_1707|Compound:st032323|FD-Score:5.92|P-value:5.32E-8 ID:SGTC_1743|Compound:st037606|FD-Score:3.59|P-value:6.51E-4 ID:SGTC_1755|Compound:st041960|FD-Score:5.79|P-value:9.96E-8 ID:SGTC_1757|Compound:st041620|FD-Score:-4.20|P-value:7.12E-5 ID:SGTC_1784|Compound:st039334|FD-Score:-3.96|P-value:1.66E-4 ID:SGTC_1786|Compound:berberine|FD-Score:5.44|P-value:5.03E-7 ID:SGTC_1836|Compound:st055642|FD-Score:-3.49|P-value:7.73E-4 ID:SGTC_1845|Compound:st056753|FD-Score:8.58|P-value:6.01E-15 ID:SGTC_1856|Compound:st057054|FD-Score:-3.71|P-value:3.81E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.55|P-value:7.41E-4 ID:SGTC_1961|Compound:st076550|FD-Score:6.44|P-value:3.59E-9 ID:SGTC_1963|Compound:st076565|FD-Score:3.67|P-value:5.03E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.00|P-value:1.64E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.96|P-value:1.92E-4 ID:SGTC_2064|Compound:5220758|FD-Score:4.55|P-value:2.24E-5 ID:SGTC_2066|Compound:5222451|FD-Score:-3.87|P-value:2.26E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.47|P-value:9.33E-4 ID:SGTC_2231|Compound:micatex|FD-Score:-3.41|P-value:9.99E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.93|P-value:2.11E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.78|P-value:3.48E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:4.16|P-value:9.43E-5 ID:SGTC_2445|Compound:5742597|FD-Score:-3.49|P-value:7.88E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:4.21|P-value:8.02E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.58|P-value:1.98E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:4.68|P-value:1.33E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.35|P-value:4.66E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.88|P-value:2.55E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-3.59|P-value:5.63E-4 ID:SGTC_2832|Compound:7992762|FD-Score:3.90|P-value:2.33E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.68|P-value:1.33E-5 ID:SGTC_2884|Compound:9058502|FD-Score:4.15|P-value:9.93E-5 ID:SGTC_2952|Compound:9073731|FD-Score:6.88|P-value:3.18E-10 ID:SGTC_2967|Compound:9089538|FD-Score:5.34|P-value:8.15E-7 ID:SGTC_2968|Compound:9090663|FD-Score:3.48|P-value:8.99E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.66|P-value:5.21E-4 ID:SGTC_3089|Compound:9117425|FD-Score:-3.85|P-value:2.41E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.54|P-value:7.43E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-3.41|P-value:9.89E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-3.81|P-value:2.74E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.96|P-value:1.94E-4 ID:SGTC_3255|Compound:9139072|FD-Score:3.80|P-value:3.31E-4 ID:SGTC_3300|Compound:9124615|FD-Score:3.52|P-value:7.96E-4 ID:SGTC_53|Compound:0973-0023|FD-Score:3.47|P-value:9.42E-4 ID:SGTC_58|Compound:0957-0393|FD-Score:6.24|P-value:1.00E-8 ID:SGTC_92|Compound:0063-0024|FD-Score:4.85|P-value:6.56E-6 ID:SGTC_150|Compound:3970-0969|FD-Score:3.84|P-value:2.88E-4 ID:SGTC_168|Compound:3970-0862|FD-Score:5.23|P-value:1.33E-6 ID:SGTC_429|Compound:1112-0049|FD-Score:3.68|P-value:4.85E-4 ID:SGTC_451|Compound:chloral hydrate|FD-Score:4.83|P-value:7.33E-6 ID:SGTC_457|Compound:grayanotoxin iii|FD-Score:3.64|P-value:5.57E-4 ID:SGTC_580|Compound:1123-0028|FD-Score:3.93|P-value:2.15E-4 ID:SGTC_764|Compound:1951-2361|FD-Score:-4.41|P-value:3.20E-5 ID:SGTC_766|Compound:1391-0721|FD-Score:3.48|P-value:9.04E-4 ID:SGTC_854|Compound:0422-0053|FD-Score:4.47|P-value:3.06E-5 ID:SGTC_885|Compound:0865-0106|FD-Score:3.58|P-value:6.58E-4 ID:SGTC_894|Compound:0960-0238|FD-Score:3.51|P-value:8.28E-4 ID:SGTC_1078|Compound:idebenone|FD-Score:4.11|P-value:1.15E-4 ID:SGTC_1194|Compound:1488-0194|FD-Score:3.49|P-value:8.84E-4 ID:SGTC_1231|Compound:0242-0792|FD-Score:3.50|P-value:8.58E-4 ID:SGTC_1254|Compound:0550-0035|FD-Score:4.21|P-value:7.94E-5 ID:SGTC_1276|Compound:0871-0068|FD-Score:4.67|P-value:1.40E-5 ID:SGTC_1375|Compound:2910-0797|FD-Score:3.52|P-value:8.12E-4 ID:SGTC_1497|Compound:4476-2978|FD-Score:3.71|P-value:4.36E-4 ID:SGTC_1538|Compound:2',3',6-trimethoxyflavanone|FD-Score:5.46|P-value:4.65E-7 ID:SGTC_1562|Compound:morin hydrate|FD-Score:3.49|P-value:8.90E-4 ID:SGTC_1636|Compound:st007707|FD-Score:4.62|P-value:1.67E-5 ID:SGTC_1673|Compound:st016611|FD-Score:-3.71|P-value:3.89E-4 ID:SGTC_1677|Compound:st019204|FD-Score:-4.29|P-value:5.20E-5 ID:SGTC_1707|Compound:st032323|FD-Score:5.92|P-value:5.32E-8 ID:SGTC_1743|Compound:st037606|FD-Score:3.59|P-value:6.51E-4 ID:SGTC_1755|Compound:st041960|FD-Score:5.79|P-value:9.96E-8 ID:SGTC_1757|Compound:st041620|FD-Score:-4.20|P-value:7.12E-5 ID:SGTC_1784|Compound:st039334|FD-Score:-3.96|P-value:1.66E-4 ID:SGTC_1786|Compound:berberine|FD-Score:5.44|P-value:5.03E-7 ID:SGTC_1836|Compound:st055642|FD-Score:-3.49|P-value:7.73E-4 ID:SGTC_1845|Compound:st056753|FD-Score:8.58|P-value:6.01E-15 ID:SGTC_1856|Compound:st057054|FD-Score:-3.71|P-value:3.81E-4 ID:SGTC_1858|Compound:4-chloro-8-(diethylamino)pyrano[3,2-c]chromene-2,5-dione|FD-Score:3.55|P-value:7.41E-4 ID:SGTC_1961|Compound:st076550|FD-Score:6.44|P-value:3.59E-9 ID:SGTC_1963|Compound:st076565|FD-Score:3.67|P-value:5.03E-4 ID:SGTC_2006|Compound:4011487|FD-Score:4.00|P-value:1.64E-4 ID:SGTC_2007|Compound:4011539|FD-Score:3.96|P-value:1.92E-4 ID:SGTC_2064|Compound:5220758|FD-Score:4.55|P-value:2.24E-5 ID:SGTC_2066|Compound:5222451|FD-Score:-3.87|P-value:2.26E-4 ID:SGTC_2188|Compound:6126565|FD-Score:3.47|P-value:9.33E-4 ID:SGTC_2231|Compound:micatex|FD-Score:-3.41|P-value:9.99E-4 ID:SGTC_2306|Compound:7482397|FD-Score:3.93|P-value:2.11E-4 ID:SGTC_2310|Compound:7668677|FD-Score:3.78|P-value:3.48E-4 ID:SGTC_2422|Compound:chlorphenesin carbamate|FD-Score:4.16|P-value:9.43E-5 ID:SGTC_2445|Compound:5742597|FD-Score:-3.49|P-value:7.88E-4 ID:SGTC_2515|Compound:chrysin|FD-Score:4.21|P-value:8.02E-5 ID:SGTC_2575|Compound:plumbagin|FD-Score:4.58|P-value:1.98E-5 ID:SGTC_2576|Compound:guaiol|FD-Score:4.68|P-value:1.33E-5 ID:SGTC_2598|Compound:perillyl alcohol|FD-Score:4.35|P-value:4.66E-5 ID:SGTC_2612|Compound:dictamnine|FD-Score:3.88|P-value:2.55E-4 ID:SGTC_2625|Compound:euphol|FD-Score:-3.59|P-value:5.63E-4 ID:SGTC_2832|Compound:7992762|FD-Score:3.90|P-value:2.33E-4 ID:SGTC_2881|Compound:9058953|FD-Score:4.68|P-value:1.33E-5 ID:SGTC_2884|Compound:9058502|FD-Score:4.15|P-value:9.93E-5 ID:SGTC_2952|Compound:9073731|FD-Score:6.88|P-value:3.18E-10 ID:SGTC_2967|Compound:9089538|FD-Score:5.34|P-value:8.15E-7 ID:SGTC_2968|Compound:9090663|FD-Score:3.48|P-value:8.99E-4 ID:SGTC_2980|Compound:9014342|FD-Score:3.66|P-value:5.21E-4 ID:SGTC_3089|Compound:9117425|FD-Score:-3.85|P-value:2.41E-4 ID:SGTC_3108|Compound:9121785|FD-Score:3.54|P-value:7.43E-4 ID:SGTC_3148|Compound:9097893|FD-Score:-3.41|P-value:9.89E-4 ID:SGTC_3196|Compound:9111891|FD-Score:-3.81|P-value:2.74E-4 ID:SGTC_3213|Compound:9129884|FD-Score:3.96|P-value:1.94E-4 ID:SGTC_3255|Compound:9139072|FD-Score:3.80|P-value:3.31E-4 ID:SGTC_3300|Compound:9124615|FD-Score:3.52|P-value:7.96E-4

Top fitness defect scores for YHR154W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_1845 st056753 8.58 3.0 µM 6.01E-15 TSC3-RPN4 4 4.79E-18
2 SGTC_2952 9073731 6.88 13.8 µM 3.18E-10 tubulin folding & SWR complex 5 3.06E-12
3 SGTC_1961 st076550 6.44 68.7 µM 3.59E-9 3 6.01E-11
4 SGTC_58 0957-0393 6.24 9.0 µM 1.00E-8 cell wall 201 2.13E-10
5 SGTC_1707 st032323 5.92 6.3 µM 5.32E-8 DNA damage response 22 1.65E-9
6 SGTC_1755 st041960 5.79 45.2 µM 9.96E-8 6 3.56E-9
7 SGTC_1538 2',3',6-trimethoxyflavanone 5.46 63.6 µM 4.65E-7 3 2.35E-8
8 SGTC_1786 berberine 5.44 21.6 µM 5.03E-7 60S ribosome export 10 2.59E-8
9 SGTC_2967 9089538 5.34 49.9 µM 8.15E-7 8 4.69E-8
10 SGTC_168 3970-0862 5.23 530.5 µM 1.33E-6 65 8.54E-8
11 SGTC_92 0063-0024 4.85 660.9 µM 6.56E-6 3 6.05E-7
12 SGTC_451 chloral hydrate 4.83 100.0 µM 7.33E-6 28 6.93E-7
13 SGTC_2576 guaiol 4.68 23.1 µM 1.33E-5 9 1.43E-6
14 SGTC_2881 9058953 4.68 52.0 µM 1.33E-5 10 1.44E-6
15 SGTC_1276 0871-0068 4.67 94.2 µM 1.40E-5 11 1.53E-6
16 SGTC_1636 st007707 4.62 68.9 µM 1.67E-5 36 1.90E-6
17 SGTC_2575 plumbagin 4.58 510.0 nM 1.98E-5 superoxide 26 2.33E-6
18 SGTC_2064 5220758 4.55 200.0 µM 2.24E-5 20 2.72E-6
19 SGTC_854 0422-0053 4.47 192.0 µM 3.06E-5 PDR1 10 3.98E-6
20 SGTC_2598 perillyl alcohol 4.35 100.0 µM 4.66E-5 21 6.67E-6

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.156 1.26E-19 YJL047C RTT101 hom Cullin subunit of a Roc1p-dependent E3 ubiquitin ligase complex with a role in anaphase progression; implicated in Mms22-dependent DNA repair; involved with Mms1p in nonfunctional rRNA decay; modified by the ubiquitin-like protein, Rub1p
0.147 1.05E-17 YBR098W MMS4 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in recombination, DNA repair, and joint molecule formation/resolution during meiotic recombination; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.134 6.61E-15 YDL059C RAD59 hom Protein involved DNA double-strand break repair; repairs breaks in DNA during vegetative growth via recombination and single-strand annealing; anneals complementary single-stranded DNA; forms nuclear foci upon DNA replication stress; required for loading of Rad52p to DSBs; paralog of Rad52p
0.123 8.57E-13 YBR292C_d YBR292C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; YBR292C is not an essential gene
0.118 6.43E-12 YDR386W MUS81 hom Subunit of the structure-specific Mms4p-Mus81p endonuclease that cleaves branched DNA; involved in DNA repair, replication fork stability, and joint molecule formation/resolution during meiotic recombination; helix-hairpin-helix protein; phosphorylation of the non-catalytic subunit Mms4p by Cdc28p and Cdcp during mitotic cell cycle activates the function of Mms4p-Mus81p
0.118 7.24E-12 YNL211C_p YNL211C_p hom Putative protein of unknown function; green fluorescent protein (GFP)-fusion protein localizes to mitochondria; YNL211C is not an essential gene
0.110 1.65E-10 YBR099C_d YBR099C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; completely overlaps the verified gene MMS4
0.107 5.14E-10 YDR004W RAD57 hom Protein that stimulates strand exchange by stabilizing the binding of Rad51p to single-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; forms heterodimer with Rad55p
0.105 9.52E-10 YOR231W MKK1 hom MAPKK involved in the protein kinase C signaling pathway; involved in control of cell integrity; upon activation by Bck1p phosphorylates downstream target, Slt2p; functionally redundant with Mkk2p; MKK1 has a paralog, MKK2, that arose from the whole genome duplication
0.104 1.76E-9 YDR205W MSC2 hom Endoplasmic reticulum zinc transporter; part of a heterodimeric transporter with Zrg17p that transfers zinc from the cytosol to the ER lumen; member of the cation diffusion facilitator family of efflux pumps; localizes to ER and nucleus; mutations affect the cellular distribution of zinc and also confer defects in meiotic recombination between homologous chromatids
0.100 5.55E-9 YGR223C HSV2 hom Phosphatidylinositol 3,5-bisphosphate-binding protein, plays a role in micronucleophagy; belongs to the PROPPIN family of proteins; predicted to fold as a seven-bladed beta-propeller; displays punctate cytoplasmic localization
0.097 1.74E-8 YNL314W DAL82 hom Positive regulator of allophanate inducible genes; binds a dodecanucleotide sequence upstream of all genes that are induced by allophanate; contains an UISALL DNA-binding, a transcriptional activation, and a coiled-coil domain
0.095 3.30E-8 YGR058W PEF1 hom Penta-EF-hand protein required for polar bud growth and cell wall abscission; binds calcium and zinc with different affinity; localizes to bud site in G1, bud neck in G2; binds to Sec31p and modulates COPII coat assembly
0.094 4.71E-8 YGL163C RAD54 hom DNA-dependent ATPase that stimulates strand exchange; modifies the topology of double-stranded DNA; involved in the recombinational repair of double-strand breaks in DNA during vegetative growth and meiosis; member of the SWI/SNF family; forms nuclear foci upon DNA replication stress
0.092 8.38E-8 YGR217W CCH1 hom Voltage-gated high-affinity calcium channel involved in calcium influx in response to some environmental stresses as well as exposure to mating pheromones; interacts and co-localizes with Mid1p, suggesting Cch1p and Mid1p function together