YHR164C / DNA2

Tripartite DNA replication factor; has single-stranded DNA-dependent ATPase, ATP-dependent nuclease, and helicase activities; required for Okazaki fragment processing; involved in DNA repair; cell-cycle dependent localization; forms nuclear foci upon DNA replication stress

Zygosity: Heterozygous strain
fixedexpanded
Profile for YHR164C / DNA2

Click on Significant Values for Screen Details ID:SGTC_275|Compound:trichlorophene|FD-Score:2.91|P-value:5.93E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.65|P-value:7.87E-14 ID:SGTC_373|Compound:0844-0104|FD-Score:3.63|P-value:2.64E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:3.10|P-value:2.76E-4 ID:SGTC_441|Compound:propafenone|FD-Score:-4.46|P-value:3.89E-7 ID:SGTC_442|Compound:cape|FD-Score:-3.62|P-value:3.08E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-3.18|P-value:2.18E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:2.97|P-value:4.66E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.52|P-value:4.35E-5 ID:SGTC_622|Compound:1348-1566|FD-Score:2.95|P-value:5.00E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:2.78|P-value:9.86E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.44|P-value:7.81E-10 ID:SGTC_790|Compound:1575-0012|FD-Score:2.87|P-value:7.02E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:2.94|P-value:5.32E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.79|P-value:1.23E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.83|P-value:8.03E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:-3.02|P-value:4.17E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-2.93|P-value:5.78E-4 ID:SGTC_1736|Compound:st036774|FD-Score:3.78|P-value:1.30E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-3.87|P-value:8.89E-6 ID:SGTC_2402|Compound:5553605|FD-Score:3.62|P-value:2.85E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.90|P-value:7.07E-6 ID:SGTC_2625|Compound:euphol|FD-Score:4.02|P-value:3.81E-6 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.91|P-value:6.66E-6 ID:SGTC_2792|Compound:7535235|FD-Score:6.41|P-value:5.24E-13 ID:SGTC_2793|Compound:7325131|FD-Score:4.02|P-value:3.84E-6 ID:SGTC_2801|Compound:7907800|FD-Score:3.51|P-value:4.62E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.19|P-value:1.90E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-3.53|P-value:4.59E-5 ID:SGTC_3203|Compound:9113543|FD-Score:-3.47|P-value:6.02E-5 ID:SGTC_3206|Compound:9114350|FD-Score:-2.96|P-value:5.32E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-2.88|P-value:7.23E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-3.60|P-value:3.34E-5 ID:SGTC_275|Compound:trichlorophene|FD-Score:2.91|P-value:5.93E-4 ID:SGTC_372|Compound:0155-0182|FD-Score:-6.65|P-value:7.87E-14 ID:SGTC_373|Compound:0844-0104|FD-Score:3.63|P-value:2.64E-5 ID:SGTC_376|Compound:3970-0862|FD-Score:3.10|P-value:2.76E-4 ID:SGTC_441|Compound:propafenone|FD-Score:-4.46|P-value:3.89E-7 ID:SGTC_442|Compound:cape|FD-Score:-3.62|P-value:3.08E-5 ID:SGTC_454|Compound:lyso-paf C-16|FD-Score:-3.18|P-value:2.18E-4 ID:SGTC_540|Compound:1187-0821|FD-Score:2.97|P-value:4.66E-4 ID:SGTC_571|Compound:k831-0106|FD-Score:3.52|P-value:4.35E-5 ID:SGTC_622|Compound:1348-1566|FD-Score:2.95|P-value:5.00E-4 ID:SGTC_623|Compound:k072-0108|FD-Score:2.78|P-value:9.86E-4 ID:SGTC_778|Compound:3456-2434|FD-Score:-5.44|P-value:7.81E-10 ID:SGTC_790|Compound:1575-0012|FD-Score:2.87|P-value:7.02E-4 ID:SGTC_827|Compound:3253-0474|FD-Score:2.94|P-value:5.32E-4 ID:SGTC_969|Compound:1313-0238|FD-Score:3.79|P-value:1.23E-5 ID:SGTC_1106|Compound:tegaserod maleate|FD-Score:2.83|P-value:8.03E-4 ID:SGTC_1175|Compound:0741-0056|FD-Score:-3.02|P-value:4.17E-4 ID:SGTC_1353|Compound:1498-1206|FD-Score:-2.93|P-value:5.78E-4 ID:SGTC_1736|Compound:st036774|FD-Score:3.78|P-value:1.30E-5 ID:SGTC_1860|Compound:st056228|FD-Score:-3.87|P-value:8.89E-6 ID:SGTC_2402|Compound:5553605|FD-Score:3.62|P-value:2.85E-5 ID:SGTC_2574|Compound:2',4'-dihydroxychalcone|FD-Score:3.90|P-value:7.07E-6 ID:SGTC_2625|Compound:euphol|FD-Score:4.02|P-value:3.81E-6 ID:SGTC_2628|Compound:lathosterol|FD-Score:3.91|P-value:6.66E-6 ID:SGTC_2792|Compound:7535235|FD-Score:6.41|P-value:5.24E-13 ID:SGTC_2793|Compound:7325131|FD-Score:4.02|P-value:3.84E-6 ID:SGTC_2801|Compound:7907800|FD-Score:3.51|P-value:4.62E-5 ID:SGTC_2831|Compound:7999036|FD-Score:3.19|P-value:1.90E-4 ID:SGTC_3006|Compound:9075424|FD-Score:-3.53|P-value:4.59E-5 ID:SGTC_3203|Compound:9113543|FD-Score:-3.47|P-value:6.02E-5 ID:SGTC_3206|Compound:9114350|FD-Score:-2.96|P-value:5.32E-4 ID:SGTC_3210|Compound:9128157|FD-Score:-2.88|P-value:7.23E-4 ID:SGTC_3352|Compound:9151559|FD-Score:-3.60|P-value:3.34E-5

Top fitness defect scores for YHR164C deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
Screen
Clearance
1 SGTC_2792 7535235 6.41 81.8 µM 5.24E-13 fatty acid desaturase (OLE1) 1 7.51E-11 0.77
2 SGTC_2625 euphol 4.02 100.0 µM 3.81E-6 9 2.90E-5 0.26
3 SGTC_2793 7325131 4.02 81.8 µM 3.84E-6 6 2.92E-5 0.10
4 SGTC_2628 lathosterol 3.91 100.0 µM 6.66E-6 7 4.58E-5 0.20
5 SGTC_2574 2',4'-dihydroxychalcone 3.90 14.6 µM 7.07E-6 10 4.81E-5 0.07
6 SGTC_969 1313-0238 3.79 134.0 µM 1.23E-5 mitochondrial processes 21 7.55E-5 0.08
7 SGTC_1736 st036774 3.78 51.0 µM 1.30E-5 4 7.89E-5 0.27
8 SGTC_373 0844-0104 3.63 25.9 µM 2.64E-5 ERAD & cell cycle 15 1.41E-4 0.00
9 SGTC_2402 5553605 3.62 200.0 µM 2.85E-5 9 1.50E-4 0.04
10 SGTC_571 k831-0106 3.52 44.2 µM 4.35E-5 10 2.12E-4 0.01
11 SGTC_2801 7907800 3.51 81.8 µM 4.62E-5 9 2.23E-4 0.16
12 SGTC_2831 7999036 3.19 19.5 µM 1.90E-4 12 7.11E-4 0.01
13 SGTC_376 3970-0862 3.10 59.1 µM 2.76E-4 21 9.63E-4 0.01
14 SGTC_540 1187-0821 2.97 17.3 µM 4.66E-4 18 0.00148 0.06
15 SGTC_622 1348-1566 2.95 26.3 µM 5.00E-4 16 0.00157 0.02
16 SGTC_827 3253-0474 2.94 693.0 µM 5.32E-4 15 0.00165 6.37E-4
17 SGTC_275 trichlorophene 2.91 5.7 µM 5.93E-4 mitochondrial stress 8 0.00180 0.01
18 SGTC_790 1575-0012 2.87 79.1 µM 7.02E-4 TSC3-RPN4 18 0.00207 0.12
19 SGTC_1106 tegaserod maleate 2.83 11.7 µM 8.03E-4 13 0.00232 0.11
20 SGTC_623 k072-0108 2.78 16.9 µM 9.86E-4 heme biosynthesis & mitochondrial translocase 36 0.00274 0.02

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.181 3.10E-26 YEL019C MMS21 het SUMO ligase and component of the SMC5-SMC6 complex; this complex plays a key role in the removal of X-shaped DNA structures that arise between sister chromatids during DNA replication and repair; mutants are sensitive to methyl methanesulfonate and show increased spontaneous mutation and mitotic recombination
0.162 3.12E-21 YLL011W SOF1 het Essential protein required for biogenesis of 40S (small) ribosomal subunit; has similarity to the beta subunit of trimeric G-proteins and the splicing factor Prp4p
0.156 1.16E-19 YNL043C_d YNL043C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; partially overlaps the verified gene YIP3/YNL044W
0.152 7.37E-19 YGR245C SDA1 het Highly conserved nuclear protein required for actin cytoskeleton organization and passage through Start, plays a critical role in G1 events, binds Nap1p, also involved in 60S ribosome biogenesis
0.140 3.92E-16 YML046W PRP39 het U1 snRNP protein involved in splicing, contains multiple tetriatricopeptide repeats
0.132 1.95E-14 YLR129W DIP2 het Nucleolar protein, specifically associated with the U3 snoRNA, part of the large ribonucleoprotein complex known as the small subunit (SSU) processome, required for 18S rRNA biogenesis, part of the active pre-rRNA processing complex
0.124 5.23E-13 YKL153W_d YKL153W_d het Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data; transcription of both YLK153W and the overlapping essential gene GPM1 is reduced in the gcr1 null mutant
0.123 7.16E-13 YGL074C_d YGL074C_d het Dubious open reading frame unlikely to encode a functional protein; overlaps 5' end of essential HSF1 gene encoding heat shock transcription factor
0.121 2.21E-12 YOR029W_d YOR029W_d hom Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.119 4.49E-12 YLR167W RPS31 het Fusion protein cleaved to yield ribosomal protein S31 and ubiquitin; ubiquitin may facilitate assembly of the ribosomal protein into ribosomes; interacts genetically with translation factor eIF2B; homologous to mammalian ribosomal protein S27A, no bacterial homolog
0.118 5.73E-12 YGL044C RNA15 het Component of the cleavage and polyadenylation factor I (CF I); CF 1, composed of the CF 1A complex (Rna14p, Rna15p, Clp1p, Pcf11p) and Hrp1, is involved in cleavage and polyadenylation of mRNA 3' ends; interacts with the A-rich polyadenylation signal in complex with Rna14p and Hrp1p; mutant displays reduced transcription elongation in the G-less-based run-on (GLRO) assay; required for gene looping
0.116 1.47E-11 YPL231W FAS2 het Alpha subunit of fatty acid synthetase, which catalyzes the synthesis of long-chain saturated fatty acids; contains the acyl-carrier protein domain and beta-ketoacyl reductase, beta-ketoacyl synthase and self-pantetheinylation activities
0.114 3.09E-11 YGR074W SMD1 het Core Sm protein Sm D1; part of heteroheptameric complex (with Smb1p, Smd2p, Smd3p, Sme1p, Smx3p, and Smx2p) that is part of the spliceosomal U1, U2, U4, and U5 snRNPs; homolog of human Sm D1; protein abundance increases in response to DNA replication stress
0.111 1.01E-10 YER127W LCP5 het Essential protein involved in maturation of 18S rRNA; depletion leads to inhibited pre-rRNA processing and reduced polysome levels; localizes primarily to the nucleolus
0.111 1.11E-10 YOL026C MIM1 het Mitochondrial protein required for outer membrane protein import; cooperates with Tom70p to import the subset of proteins with multiple alpha-helical transmembrane segments, including Ugo1p, Tom20p, and others; present in a complex with Mim2p in the outer membrane that may create a local environment to facilitate membrane insertion of substrate proteins; also has a role in assembly of Tom20p into the TOM complex