YHR171W / ATG7

Autophagy-related protein and dual specificity member of the E1 family of ubiquitin-activating enzymes; mediates the conjugation of Atg12p with Atg5p and Atg8p with phosphatidylethanolamine, required steps in autophagosome formation

Zygosity: Homozygous strain
fixedexpanded
Profile for YHR171W / ATG7

Click on Significant Values for Screen Details ID:SGTC_93|Compound:0109-0045|FD-Score:4.57|P-value:3.89E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:4.33|P-value:9.33E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:4.89|P-value:1.17E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:5.79|P-value:2.63E-7 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.57|P-value:6.91E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.72|P-value:4.37E-4 ID:SGTC_456|Compound:gingerol|FD-Score:3.87|P-value:4.31E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.98|P-value:8.38E-6 ID:SGTC_693|Compound:4161-2696|FD-Score:-3.56|P-value:7.22E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:3.78|P-value:5.74E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.86|P-value:4.41E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:4.56|P-value:4.06E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:3.80|P-value:5.34E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.69|P-value:7.38E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.99|P-value:2.92E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:5.11|P-value:4.95E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.08|P-value:2.17E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.77|P-value:1.86E-5 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.07|P-value:2.24E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.88|P-value:4.15E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-4.42|P-value:4.02E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-4.71|P-value:1.34E-5 ID:SGTC_1713|Compound:st033146|FD-Score:-4.57|P-value:2.30E-5 ID:SGTC_1746|Compound:st038117|FD-Score:-5.09|P-value:2.87E-6 ID:SGTC_1897|Compound:st060201|FD-Score:5.23|P-value:2.93E-6 ID:SGTC_1910|Compound:st062352|FD-Score:4.11|P-value:1.97E-4 ID:SGTC_2092|Compound:5303694|FD-Score:3.88|P-value:4.18E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.84|P-value:4.64E-4 ID:SGTC_2113|Compound:5491891|FD-Score:6.56|P-value:6.13E-9 ID:SGTC_2380|Compound:9035439|FD-Score:3.85|P-value:4.58E-4 ID:SGTC_2481|Compound:5478948|FD-Score:8.13|P-value:7.72E-13 ID:SGTC_2488|Compound:5268135|FD-Score:3.76|P-value:6.07E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:5.94|P-value:1.28E-7 ID:SGTC_2686|Compound:alizarin|FD-Score:4.24|P-value:1.27E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-3.72|P-value:4.35E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.48|P-value:8.96E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.89|P-value:4.00E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.92|P-value:2.24E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.55|P-value:7.25E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.79|P-value:5.48E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.88|P-value:4.20E-4 ID:SGTC_2887|Compound:9044784|FD-Score:4.49|P-value:5.18E-5 ID:SGTC_3032|Compound:9089427|FD-Score:3.74|P-value:6.36E-4 ID:SGTC_3094|Compound:9117742|FD-Score:4.03|P-value:2.58E-4 ID:SGTC_3198|Compound:9112019|FD-Score:4.61|P-value:3.42E-5 ID:SGTC_3200|Compound:9114385|FD-Score:5.22|P-value:3.07E-6 ID:SGTC_3233|Compound:9132340|FD-Score:4.38|P-value:7.73E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-5.25|P-value:1.49E-6 ID:SGTC_93|Compound:0109-0045|FD-Score:4.57|P-value:3.89E-5 ID:SGTC_100|Compound:0086-0128|FD-Score:4.33|P-value:9.33E-5 ID:SGTC_245|Compound:methotrexate|FD-Score:4.89|P-value:1.17E-5 ID:SGTC_249|Compound:wiskostatin|FD-Score:5.79|P-value:2.63E-7 ID:SGTC_254|Compound:nsc-64875|FD-Score:-3.57|P-value:6.91E-4 ID:SGTC_271|Compound:rotenone|FD-Score:-3.72|P-value:4.37E-4 ID:SGTC_456|Compound:gingerol|FD-Score:3.87|P-value:4.31E-4 ID:SGTC_670|Compound:4204-0025|FD-Score:4.98|P-value:8.38E-6 ID:SGTC_693|Compound:4161-2696|FD-Score:-3.56|P-value:7.22E-4 ID:SGTC_701|Compound:4424-0219|FD-Score:3.78|P-value:5.74E-4 ID:SGTC_704|Compound:1310-0270|FD-Score:3.86|P-value:4.41E-4 ID:SGTC_725|Compound:1497-0963|FD-Score:4.56|P-value:4.06E-5 ID:SGTC_788|Compound:1182-0090|FD-Score:3.80|P-value:5.34E-4 ID:SGTC_964|Compound:1287-0107|FD-Score:3.69|P-value:7.38E-4 ID:SGTC_974|Compound:1326-0144|FD-Score:3.99|P-value:2.92E-4 ID:SGTC_1203|Compound:3992-3522|FD-Score:5.11|P-value:4.95E-6 ID:SGTC_1242|Compound:0402-0103|FD-Score:4.08|P-value:2.17E-4 ID:SGTC_1248|Compound:0469-0687|FD-Score:4.77|P-value:1.86E-5 ID:SGTC_1258|Compound:0671-0043|FD-Score:4.07|P-value:2.24E-4 ID:SGTC_1510|Compound:1123-0200|FD-Score:3.88|P-value:4.15E-4 ID:SGTC_1629|Compound:st005085|FD-Score:-4.42|P-value:4.02E-5 ID:SGTC_1634|Compound:st006576|FD-Score:-4.71|P-value:1.34E-5 ID:SGTC_1713|Compound:st033146|FD-Score:-4.57|P-value:2.30E-5 ID:SGTC_1746|Compound:st038117|FD-Score:-5.09|P-value:2.87E-6 ID:SGTC_1897|Compound:st060201|FD-Score:5.23|P-value:2.93E-6 ID:SGTC_1910|Compound:st062352|FD-Score:4.11|P-value:1.97E-4 ID:SGTC_2092|Compound:5303694|FD-Score:3.88|P-value:4.18E-4 ID:SGTC_2108|Compound:5365811|FD-Score:3.84|P-value:4.64E-4 ID:SGTC_2113|Compound:5491891|FD-Score:6.56|P-value:6.13E-9 ID:SGTC_2380|Compound:9035439|FD-Score:3.85|P-value:4.58E-4 ID:SGTC_2481|Compound:5478948|FD-Score:8.13|P-value:7.72E-13 ID:SGTC_2488|Compound:5268135|FD-Score:3.76|P-value:6.07E-4 ID:SGTC_2614|Compound:arthonioic acid|FD-Score:5.94|P-value:1.28E-7 ID:SGTC_2686|Compound:alizarin|FD-Score:4.24|P-value:1.27E-4 ID:SGTC_2690|Compound:usnic acid|FD-Score:-3.72|P-value:4.35E-4 ID:SGTC_2698|Compound:st077697|FD-Score:-3.48|P-value:8.96E-4 ID:SGTC_2715|Compound:st079500|FD-Score:3.89|P-value:4.00E-4 ID:SGTC_2763|Compound:lisinopril|FD-Score:-3.92|P-value:2.24E-4 ID:SGTC_2765|Compound:myclobutanil|FD-Score:-3.55|P-value:7.25E-4 ID:SGTC_2837|Compound:9008476|FD-Score:3.79|P-value:5.48E-4 ID:SGTC_2855|Compound:9016525|FD-Score:3.88|P-value:4.20E-4 ID:SGTC_2887|Compound:9044784|FD-Score:4.49|P-value:5.18E-5 ID:SGTC_3032|Compound:9089427|FD-Score:3.74|P-value:6.36E-4 ID:SGTC_3094|Compound:9117742|FD-Score:4.03|P-value:2.58E-4 ID:SGTC_3198|Compound:9112019|FD-Score:4.61|P-value:3.42E-5 ID:SGTC_3200|Compound:9114385|FD-Score:5.22|P-value:3.07E-6 ID:SGTC_3233|Compound:9132340|FD-Score:4.38|P-value:7.73E-5 ID:SGTC_3309|Compound:9129340|FD-Score:-5.25|P-value:1.49E-6

Top fitness defect scores for YHR171W deletion by condition

RankScreen IDConditionFD ScoreConc
P-value
Response
Signature
Screen
Rank
Screen
P-value
1 SGTC_2481 5478948 8.13 45.2 µM 7.72E-13 PDR1 3 2.21E-16
2 SGTC_2113 5491891 6.56 107.0 µM 6.13E-9 TSC3-RPN4 3 2.68E-11
3 SGTC_2614 arthonioic acid 5.94 13.4 µM 1.28E-7 4 1.40E-9
4 SGTC_249 wiskostatin 5.79 11.2 µM 2.63E-7 2 3.60E-9
5 SGTC_1897 st060201 5.23 15.0 µM 2.93E-6 mitochondrial processes 8 8.28E-8
6 SGTC_3200 9114385 5.22 49.5 µM 3.07E-6 10 8.80E-8
7 SGTC_1203 3992-3522 5.11 103.0 µM 4.95E-6 11 1.64E-7
8 SGTC_670 4204-0025 4.98 119.0 µM 8.38E-6 copper-dependent oxidative stress 13 3.26E-7
9 SGTC_245 methotrexate 4.89 289.6 µM 1.17E-5 22 5.04E-7
10 SGTC_1248 0469-0687 4.77 58.6 µM 1.86E-5 12 9.17E-7
11 SGTC_3198 9112019 4.61 49.5 µM 3.42E-5 mitochondrial processes 7 2.03E-6
12 SGTC_93 0109-0045 4.57 59.2 µM 3.89E-5 DNA damage response 23 2.41E-6
13 SGTC_725 1497-0963 4.56 19.0 µM 4.06E-5 copper-dependent oxidative stress 12 2.54E-6
14 SGTC_2887 9044784 4.49 58.4 µM 5.18E-5 fatty acid desaturase (OLE1) 18 3.49E-6
15 SGTC_3233 9132340 4.38 49.5 µM 7.73E-5 14 5.87E-6
16 SGTC_100 0086-0128 4.33 49.6 µM 9.33E-5 copper-dependent oxidative stress 35 7.50E-6
17 SGTC_2686 alizarin 4.24 95.4 µM 1.27E-4 RPP1 & pyrimidine depletion 25 1.12E-5
18 SGTC_1910 st062352 4.11 60.4 µM 1.97E-4 31 1.98E-5
19 SGTC_1242 0402-0103 4.08 60.1 µM 2.17E-4 24 2.25E-5
20 SGTC_1258 0671-0043 4.07 57.1 µM 2.24E-4 23 2.35E-5

Download Fitness data (tab-delimited text)  (excel)
Cofit Genes
Download Cofitness data (tab-delimited text)  (excel)

Correlation pval ORF Gene Zygosity Description
0.101 5.54E-9 YER091C MET6 hom Cobalamin-independent methionine synthase, involved in methionine biosynthesis and regeneration; requires a minimum of two glutamates on the methyltetrahydrofolate substrate, similar to bacterial metE homologs
0.083 1.68E-6 YGL124C MON1 hom Protein required for fusion of cvt-vesicles and autophagosomes with the vacuole; associates, as a complex with Ccz1p, with a perivacuolar compartment; potential Cdc28p substrate
0.081 2.42E-6 YGL147C RPL9A hom Ribosomal 60S subunit protein L9A; nearly identical to paralog Rpl9Bp; homologous to mammalian ribosomal protein L9 and bacterial L6
0.080 3.99E-6 YBR152W SPP381 het mRNA splicing factor, component of U4/U6.U5 tri-snRNP; interacts genetically and physically with Prp38p
0.079 4.90E-6 YPR001W CIT3 hom Dual specificity mitochondrial citrate and methylcitrate synthase; catalyzes the condensation of acetyl-CoA and oxaloacetate to form citrate and that of propionyl-CoA and oxaloacetate to form 2-methylcitrate
0.078 5.91E-6 YKR103W NFT1 hom Putative transporter of the multidrug resistance-associated protein (MRP) subfamily; adjacent ORFs YKR103W and YKR104W are merged in different strain backgrounds.
0.078 6.65E-6 YMR016C SOK2 hom Nuclear protein that negatively regulates pseudohyphal differentiation; plays a regulatory role in the cyclic AMP (cAMP)-dependent protein kinase (PKA) signal transduction pathway; SOK2 has a paralog, PHD1, that arose from the whole genome duplication
0.077 8.42E-6 YBR131W CCZ1 hom Protein involved in vacuolar assembly, essential for autophagy and the cytoplasm-to-vacuole pathway
0.075 1.26E-5 YNR053C NOG2 het Putative GTPase that associates with pre-60S ribosomal subunits in the nucleolus and is required for their nuclear export and maturation
0.074 1.97E-5 YBR232C_d YBR232C_d hom Dubious open reading frame unlikely to encode a protein, based on available experimental and comparative sequence data
0.073 2.03E-5 YGR148C RPL24B hom Ribosomal 60S subunit protein L24B; not essential for translation but may be required for normal translation rate; homologous to mammalian ribosomal protein L24, no bacterial homolog; RPL24B has a paralog, RPL24A, that arose from the whole genome duplication
0.073 2.54E-5 YDR210W_p YDR210W_p hom Predicted tail-anchored plasma membrane protein containing a conserved CYSTM module; related proteins in other organisms may be involved in response to stress; green fluorescent protein (GFP)-fusion protein localizes to the cell periphery
0.071 3.73E-5 YLL048C YBT1 hom Transporter of the ATP-binding cassette (ABC) family; involved in bile acid transport; negative regulator of vacuole fusion; regulates the release of lumenal Ca2+ stores; similar to mammalian bile transporters
0.070 5.01E-5 YIL136W OM45 hom Mitochondrial outer membrane protein of unknown function; major constituent of the outer membrane, located on the outer (cytosolic) face; protein abundance increases in response to DNA replication stress
0.068 7.39E-5 YBR264C YPT10 hom Rab family GTP-binding protein that contains the PEST signal sequence specific for proteolytic enzymes; may be involved in vesicular transport; overexpression leads to accumulation of Golgi-like cisternae with budding vesicles