HIPHOP chemogenomics database

YHR172W / SPC97

Component of the microtubule-nucleating Tub4p (gamma-tubulin) complex; interacts with Spc110p at the spindle pole body (SPB) inner plaque and with Spc72p at the SPB outer plaque

Zygosity: Heterozygous strain

fixedexpanded
Profile for YHR172W / SPC97

Click on Significant Values for Screen Details

Top fitness defect scores for YHR172W deletion by condition

Rank	Screen ID	Condition	FD Score	Conc	P-value	Response Signature	Screen Rank	Screen P-value	Screen Clearance
1	SGTC_163	1327-0036	12.20	7.5 µM	4.36E-31		8	1.31E-34	0.22
2	SGTC_264	promethazine	9.78	12.5 mM	1.27E-20	DNA intercalators	5	6.86E-23	0.47
3	SGTC_3199	9111318	8.23	49.5 µM	3.79E-15		2	9.17E-17	2.29
4	SGTC_2282	7944268	7.70	200.0 µM	1.77E-13	RPP1 & pyrimidine depletion	2	6.77E-15	0.05
5	SGTC_43	0570-0105	7.69	72.5 µM	1.90E-13	ERG2	2	7.33E-15	0.97
6	SGTC_370	0180-0345	7.65	38.7 µM	2.58E-13	DNA intercalators	1	1.03E-14	2.07
7	SGTC_2619	obliquin	7.28	87.9 µM	3.06E-12	cell wall	10	1.64E-13	0.28
8	SGTC_166	k072-0058	7.28	166.4 µM	3.14E-12	DNA intercalators	1	1.69E-13	1.98
9	SGTC_471	1-acyl-paf	7.26	4.0 µM	3.61E-12	DNA intercalators	5	1.97E-13	0.69
10	SGTC_2651	1327-0036	6.86	5.0 µM	4.74E-11		26	3.51E-12	0.01
11	SGTC_512	prima-1	6.75	270.0 µM	9.00E-11	DNA intercalators	1	7.19E-12	0.78
12	SGTC_2290	7904846	6.33	200.0 µM	1.15E-9		5	1.25E-10	1.21
13	SGTC_470	aa-861	6.07	153.0 µM	4.97E-9	sphingolipid biosynthesis & PDR1	3	6.38E-10	0.04
14	SGTC_2906	7926292	5.66	26.4 µM	4.51E-8		2	7.50E-9	1.41
15	SGTC_867	0518-0118	5.55	155.0 µM	8.18E-8	DNA intercalators	8	1.46E-8	1.00
16	SGTC_145	3737-0113	5.46	38.4 µM	1.24E-7	DNA intercalators	24	2.32E-8	0.09
17	SGTC_514	4466-0038	5.40	2.6 nM	1.74E-7	cell wall	23	3.38E-8	0.17
18	SGTC_1807	st050092	5.08	58.9 µM	7.98E-7	60S ribosome export	1	1.86E-7	0.59
19	SGTC_1926	methyl fluorone black	5.01	53.6 µM	1.12E-6	DNA intercalators	16	2.72E-7	0.03
20	SGTC_171	k064-0041	5.00	9.7 µM	1.17E-6	heme requiring	10	2.86E-7	0.42

Download Fitness data (tab-delimited text) (excel)

Cofit Genes

Download Cofitness data (tab-delimited text) (excel)

Correlation	pval	ORF	Gene	Zygosity	Description
0.365	2.06E-106	YHL039W	EFM1	hom	Lysine methyltransferase involved in the monomethylation of eEF1A (Tef1p/Tef2p); SET-domain family member; predicted involvement in ribosome biogenesis; green fluorescent protein (GFP)-fusion protein localizes to the cytoplasm
0.343	4.80E-93	YDL223C	HBT1	hom	Substrate of the Hub1p ubiquitin-like protein that localizes to the shmoo tip (mating projection); mutants are defective for mating projection formation, thereby implicating Hbt1p in polarized cell morphogenesis
0.325	4.03E-83	YMR166C_p	YMR166C_p	hom	Predicted transporter of the mitochondrial inner membrane; has similarity to human mitochondrial ATP-Mg/Pi carriers; YMR166C is not an essential gene
0.312	1.43E-76	YMR289W	ABZ2	hom	Aminodeoxychorismate lyase (4-amino-4-deoxychorismate lyase), catalyzes the third step in para-aminobenzoic acid biosynthesis; involved in folic acid biosynthesis
0.304	7.27E-73	YMR168C	CEP3	het	Essential kinetochore protein, component of the CBF3 complex that binds the CDEIII region of the centromere; contains an N-terminal Zn2Cys6 type zinc finger domain, a C-terminal acidic domain, and a putative coiled coil dimerization domain
0.297	2.34E-69	YGL200C	EMP24	hom	Component of the p24 complex; binds to GPI anchor proteins and mediates their efficient transport from the ER to the Golgi; integral membrane protein that associates with endoplasmic reticulum-derived COPII-coated vesicles
0.270	4.37E-57	YGR065C	VHT1	het	High-affinity plasma membrane H+-biotin (vitamin H) symporter; mutation results in fatty acid auxotrophy; 12 transmembrane domain containing major facilitator subfamily member; mRNA levels negatively regulated by iron deprivation and biotin
0.267	1.08E-55	YOR133W	EFT1	hom	Elongation factor 2 (EF-2), also encoded by EFT2; catalyzes ribosomal translocation during protein synthesis; contains diphthamide, the unique posttranslationally modified histidine residue specifically ADP-ribosylated by diphtheria toxin; EFT1 has a paralog, EFT2, that arose from the whole genome duplication
0.261	4.05E-53	YJL025W	RRN7	het	Component of the core factor (CF) rDNA transcription factor complex; CF is required for transcription of 35S rRNA genes by RNA polymerase I and is composed of Rrn6p, Rrn7p, and Rrn11p
0.228	1.11E-40	YGL118C_d	YGL118C_d	hom	Dubious open reading frame unlikely to encode a functional protein, based on available experimental and comparative sequence data
0.219	1.44E-37	YDR381W	YRA1	het	RNA binding protein required for export of poly(A)+ mRNA from the nucleus; proposed to couple mRNA export with 3' end processing via its interactions with Mex67p and Pcf11p; functionally redundant with Yra2p, another REF family member
0.209	1.85E-34	YFL037W	TUB2	het	Beta-tubulin; associates with alpha-tubulin (Tub1p and Tub3p) to form tubulin dimer, which polymerizes to form microtubules; mutation in human ortholog is associated with congenital fibrosis of the extraocular muscles (CFEOM) with polymicrogyria
0.208	4.42E-34	YDL058W	USO1	het	Essential protein involved in the vesicle-mediated ER to Golgi transport step of secretion; binds membranes and functions during vesicle docking to the Golgi; required for assembly of the ER-to-Golgi SNARE complex
0.198	4.36E-31	YIR008C	PRI1	het	Subunit of DNA primase, which is required for DNA synthesis and double-strand break repair
0.197	8.61E-31	YPL157W	TGS1	hom	Trimethyl guanosine synthase, conserved nucleolar methyl transferase that converts the m(7)G cap structure of snRNAs, snoRNAs, and telomerase TLC1 RNA to m(2,2,7)G; also required for nucleolar assembly and splicing of meiotic pre-mRNAs